Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,197,774	T→C	V20A (GTC→GCC)	hemE →	uroporphyrinogen decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,197,774	0	T	C	100.0%	299.4 / NA	82	V20A (GTC→GCC)	hemE	uroporphyrinogen decarboxylase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (45/37);  total (45/37)

GATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGTCACTCCAGTATGGATGATGCGCCAGGCGGGTCGCTATCTACCGGAATATAAAGCCACGCGCGCCCAGGCAGGCGATTTTATGTCGCTGTGCAAAAACGCCGAGCTGGCGTGCGAAGTGACTTTGCAACCGCT  >  NC_000913/4197641‑4197906                                                                                                                                      |                                                                                                                                     gaTACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGCCACTcc                                                                                                                                >  1:1094499/1‑140 (MQ=255) gaTACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGCCACTcc                                                                                                                                >  1:477106/1‑140 (MQ=255)   tACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGCCACTc                                                                                                                                 <  2:991788/137‑1 (MQ=255)   tACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGCCACTc                                                                                                                                 >  1:991788/1‑137 (MQ=255)   tACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGCCACTCCAg                                                                                                                              >  1:361558/1‑140 (MQ=255)   tACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCCGCTGCGCCAGCCCGTTGATGCCACTCCAg                                                                                                                              >  1:62165/1‑140 (MQ=255)      ggTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGCCACTCCAGTAt                                                                                                                           >  1:760117/1‑140 (MQ=255)      ggTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGCCACTCCAGTAt                                                                                                                           >  1:1324294/1‑140 (MQ=255)        tGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGCCACTCCAGTATgg                                                                                                                         >  2:458663/1‑140 (MQ=255)         ggCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGCCACTCCAGTATGga                                                                                                                        >  1:705382/1‑140 (MQ=255)         ggCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGCCACTCCAGTATGga                                                                                                                        >  2:779962/1‑140 (MQ=255)           cGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGCCACTc                                                                                                                                 <  1:1319417/129‑1 (MQ=255)           cGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGCCACTc                                                                                                                                 >  2:1319417/1‑129 (MQ=255)              tgtgTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGCCACTc                                                                                                                                 <  2:322034/126‑1 (MQ=255)              tgtgTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGCCACTc                                                                                                                                 >  1:322034/1‑126 (MQ=255)              tgtgTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGCCACTCCAGTATGggtga                                                                                                                     <  2:287950/138‑1 (MQ=255)              tgtgTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGCCACTCCAGTATGggtga                                                                                                                     >  1:287950/1‑138 (MQ=255)              tgtgTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGCCACTCCAGTATGGatgatg                                                                                                                   <  1:1267914/140‑1 (MQ=255)                    gggCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGCCACTCCAGTATGGATGATGCGCCAg                                                                                                             >  2:1264745/1‑140 (MQ=255)                    gggCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGCCACTCCAGTATGGATGATGCGCCAg                                                                                                             >  1:790638/1‑140 (MQ=255)                     ggCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGCCACTCCAGTATGGATGATGCGCCAgg                                                                                                            >  2:677162/1‑140 (MQ=255)                       cAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGCCACTCCAGTATGGATGATGCGCCAGGCg                                                                                                          <  2:705382/140‑1 (MQ=255)                         gagTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGCCACTCCAGTATGGATGATGCGCCAGGCggg                                                                                                        <  1:349567/140‑1 (MQ=255)                          agTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGCCACTCCAGTATGGATGATGCGCCAGGCgggc                                                                                                       >  2:1149108/1‑139 (MQ=255)                            tATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGCCACTCCAGTATGGATGATGCGCCAGGCGGGTCg                                                                                                     >  1:312975/1‑140 (MQ=255)                              tGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGCCACTCCAGTATGGATGATGCGCCAGGCGGGTCGCt                                                                                                   <  2:477106/140‑1 (MQ=255)                                 gTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGCCACTCCAGTATGGATGATGCGCCAGGCGGGTCGCTATc                                                                                                <  2:1306293/140‑1 (MQ=255)                                 gTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGCCACTCCAGTATGGATGATGCGCCAGGCGGGTCGCTATc                                                                                                <  2:790638/140‑1 (MQ=255)                                   gatgatACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGCCACTCCAGTATGGATGATGCGCCAGGCGGGTCGCTATCTa                                                                                              >  1:481519/1‑140 (MQ=255)                                    atgatACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGCCACTCCAGTATGGATGATGCGCCAGGCGGGTCGCTATCTAc                                                                                             >  2:535274/1‑140 (MQ=255)                                    atgatACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGCCACTCCAGTATGGATGATGCGCCAGGCGGGTCGCTATCTAc                                                                                             <  1:1240205/140‑1 (MQ=255)                                    atgatACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGCCACTCCAGTATGGATGATGCGCCAGGCGGGTCGCTATCTAc                                                                                             >  2:228153/1‑140 (MQ=255)                                      gatACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGCCACTCCAGTATGGATGATGCGCCAGGCGGGTCGCTATCTACCg                                                                                           <  2:1324294/140‑1 (MQ=255)                                      gatACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGCCACTCCAGTATGGATGATGCGCCAGGCGGGTCGCTATCTACCg                                                                                           <  2:1146582/140‑1 (MQ=255)                                      gatACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGCCACTCCAGTATGGATGATGCGCCAGGCGGGTCGCTATCTACCg                                                                                           >  2:567602/1‑140 (MQ=255)                                      gatACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGCCACTCCAGTATGGATGATGCGCCAGGCGGGTCGCTATCTACCg                                                                                           >  2:515162/1‑140 (MQ=255)                                      gatACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGCCACTCCAGTATGGATGATGCGCCAGGCGGGTCGCTATCTACCg                                                                                           <  2:536783/140‑1 (MQ=255)                                       atACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGCCACTCCAGTATGGATGATGCGCCAGGCGGGTCGCTATCTACCgg                                                                                          <  1:458663/140‑1 (MQ=255)                                         acacTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGCCACTCCAGTATGGATGATGCGCCAGGCGGGTCGCTATCTACCGGaa                                                                                        >  1:1140191/1‑140 (MQ=255)                                            cTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGCCACTCCAGTATGGATGATGCGCCAGGCGGGTCGCTATCTAc                                                                                             >  2:887949/1‑132 (MQ=255)                                            cTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGCCACTCCAGTATGGATGATGCGCCAGGCGGGTCGCTATCTAc                                                                                             <  1:887949/132‑1 (MQ=255)                                            cTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGCCACTCCAGTATGGATGATGCGCCAGGCGGGTCGCTATCTACCGGAatat                                                                                     >  1:516981/1‑140 (MQ=255)                                               aCCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGCCACTCCAGTATGGATGATGCGCCAGGCGGGTCGCTATCTACCGGAATATaaa                                                                                  >  2:218198/1‑140 (MQ=255)                                               aCCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGCCACTCCAGTATGGATGATGCGCCAGGCGGGTCGCTATCTACCGGAATATaaa                                                                                  >  2:1016875/1‑140 (MQ=255)                                               aCCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGCCACTCCAGTATGGATGATGCGCCAGGCGGGTCGCTATCTACCGGAATATaaa                                                                                  >  2:609666/1‑140 (MQ=255)                                               aCCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGCCACTCCAGTATGGATGATGCGCCAGGCGGGTCGCTATCTACCGGAATATaaa                                                                                  >  2:1141996/1‑140 (MQ=255)                                               aCCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGCCACTCCAGTATGGATGATGCGCCAGGCGGGTCGCTATCTACCGGAATATaaa                                                                                  >  1:1288090/1‑140 (MQ=255)                                                 cGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGCCACTCCAGTATGGATGATGCGCCAGGCGGGTCGCTATCTACCGGAATATAAAGc                                                                                <  2:1094499/140‑1 (MQ=255)                                                     tGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGCCACTCCAGTATGGATGATGCGCCAGGCGGGTCGCTATCTACCGGAATATAAAGCCAcg                                                                            <  2:1116746/140‑1 (MQ=255)                                                     tGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGCCACTCCAGTATGGATGATGCGCCAGGCGGGTCGCTATCTACCGGAATATAAAGCCAcg                                                                            <  2:760117/140‑1 (MQ=255)                                                     tGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGCCACTCCAGTATGGATGATGCGCCAGGCGGGTCGCTATCTACCGGAATATAAAGCCAcg                                                                            <  1:779962/140‑1 (MQ=255)                                                     tGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGCCACTCCAGTATGGATGATGCGCCAGGCGGGTCGCTATCTACCGGAATATAAAGCCAcg                                                                            <  1:228153/140‑1 (MQ=255)                                                     tGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGCCACTCCAGTATGGATGATGCGCCAGGCGGGTCGCTATCTACCGGAATATAAAGCCAcg                                                                            <  1:349317/140‑1 (MQ=255)                                                             tAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGCCACTCCAGTATGGATGATGCGCCAGGCGGGTCGCTATCTACCGGAATATAAAGCCACGCGCGCCca                                                                    >  1:245830/1‑140 (MQ=255)                                                               aGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGCCACTCCAGTATGGATGATGCGCCAGGCGGGTCGCTATCTACCGGAATATAAAGCCACGCGCGCCcagg                                                                  >  1:1056160/1‑140 (MQ=255)                                                                  aaCAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGCCACTCCAGTATGGATGATGCGCCAGGCGGGTCGCTATCTACCGGAATATAAAGCCACGCGCGCCcaggcag                                                               <  2:1140191/140‑1 (MQ=255)                                                                  aaCAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGCCACTCCAGTATGGATGATGCGCCAGCCGGGTCGCTATCTACCGGAATATAAAGCCACGCGCGCCcacgcag                                                               >  1:1223992/1‑140 (MQ=255)                                                                   aCAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGCCACTCCAGTATGGATGATGCGCCAGGCGGGTCGCTATCTACCGGAATATAAAGCCACGCGCGCCcaggcagg                                                              <  2:1056160/140‑1 (MQ=255)                                                                   aCAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGCCACTCCAGTATGGATGATGCGCCAGGCGGGTCGCTATCTACCGGAATATAAAGCCACGCGCGCCcaggcagg                                                              <  1:515162/140‑1 (MQ=255)                                                                    cAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGCCACTCCAGTATGGATGATGCGCCAGGCGGGTCGCTATCTACCGGAATATAAAGCCACGCGCGCCCaggcaggc                                                             <  1:606532/140‑1 (MQ=255)                                                                     aGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGCCACTCCAGTATGGATGATGCGCCAGGCGGGTCGCTATCTACCGGAATATAAAGCCAcg                                                                            <  2:989160/124‑1 (MQ=255)                                                                     aGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGCCACTCCAGTATGGATGATGCGCCAGGCGGGTCGCTATCTACCGGAATATAAAGCCAcg                                                                            >  1:989160/1‑124 (MQ=255)                                                                     aGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGCCACTCCAGTATGGATGATGCGCCAGGCGGGCCGCTATCTACCGGAATATAAAGCCACGCGCGCCCAGGCAGGCg                                                            >  1:434908/1‑140 (MQ=255)                                                                       ccAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGCCACTCCAGTATGGATGATGCGCCAGGCGGGTCGCTATCTACCGGAATATAAAGCCACGCGCGCCCAGGCAGGCGAt                                                          >  2:90918/1‑140 (MQ=255)                                                                           aaTGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGCCACTCCAGTATGGATGATGCGCCAGGCGGGTCGCTATCTACCGGAATATAAAGCCACGCGCGCCCAGGCAGGCGATTTTa                                                      >  1:497597/1‑140 (MQ=255)                                                                           aaTGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCCCCGGCCCGTTGATGCCACTCCAGTATGGATGATGCGCCAGGCGGGTCGCTATCTACCGGAATATAAAGCCACGCGCGCCCAGGCAGGCGATTTTa                                                      <  1:1290698/140‑1 (MQ=255)                                                                           aaTGACCGAACTTAAAAACGATCGTTATCTGCGGGC‑CTGCTGCGCCAGCCCGTTGATGCCACTCCAGTATGGATGATGCGCCAGGCGGGTCGCTATCTACCGGAATATAAAGCCACGCGCGCCCAGGCAGGCGATTTTAt                                                     >  2:819578/1‑140 (MQ=255)                                                                                             cGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGCCACTCCAGTATGGATGATGCGCCAGGCGGGTCGCTATCTACCGGAATATAAAGCCACGCGCGCCCAGGCAGGCGATTTTATGTCGCTGTGCAAAAACg                                    <  1:567602/140‑1 (MQ=255)                                                                                               aTCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGCCACTCCAGTATGGATGATGCGCCAGGCGGGTCGCTATCTACCGGAATATAAAGCCACGCGCGCCCAGGCAGGCGATTTTATGTCGCTGTGCAAAAACGcc                                  >  1:401328/1‑140 (MQ=255)                                                                                                tCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGCCACTCCAGTATGGATGATGCGCCAGGCGGGTCGCTATCTACCGGAATATAAAGCCACGCGCGCCCAGGCAGGCGATTTTATGTCg                                                 <  1:515260/124‑1 (MQ=255)                                                                                                tCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGCCACTCCAGTATGGATGATGCGCCAGGCGGGTCGCTATCTACCGGAATATAAAGCCACGCGCGCCCAGGCAGGCGATTTTATGTCg                                                 >  2:515260/1‑124 (MQ=255)                                                                                                  gTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGCCACTCCAGTATGGATGATGCGCCAGGCGGGTCGCTATCTACCGGAATATAAAGCCACGCGCGCCCAGGCAGGCGATTTTATGTCGCTGTGCAAAAACGCCGAg                               <  2:416697/140‑1 (MQ=255)                                                                                                         gCGGGCGCTGCTGCGCCAGCCCGTTGATGCCACTCCAGTATGGATGATGCGCCAGGCGGGTCGCTATCTACCGGAATATAAAGCCACGCGCGCCCAGGCAGGCGATTTTATGTCGCTGTGCAAAAACGCCGAGCTGGCGt                        >  2:537602/1‑140 (MQ=255)                                                                                                          cGGGCGCTGCTGCGCCAGCCCGTTGATGCCACTCCAGTATGGATGATGCGCCAGGCGGGTCGCTATCTACCGGAATATAAAGCCACGCGCGCCCAGGCAGGCGATTTTATGTCGCTGTGCAAAAACGCCGAGCTGGCGTg                       >  2:839010/1‑140 (MQ=255)                                                                                                                ctgctgCGCCAGCCCGTTGATGCCACTCCAGTATGGATGATGCGCCAGGCGGGTCGCTATCTACCGGAATATAAAGCCACGCGCGCCCAGGCAGGCGATTTTATGTCGCTGTGCAAAAACGCCGAGCTGGCGTGCGAAGt                 >  1:148316/1‑140 (MQ=255)                                                                                                                ctgctgCGCCAGCCCGTTGATGCCACTCCAGTATGGATGATGCGCCAGGCGGGTCGCTATCTACCGGAATATAAAGCCACGCGCGCCCAGGCAGGCGATTTTATGTCGCTGTGCAAAAACGCCGAGCTGGCGTGCGAAGt                 <  1:218198/140‑1 (MQ=255)                                                                                                                ctgctgCGCCAGCCCGTTGATGCCACTCCAGTATGGATGATGCGCCAGGCGGGTCGCTATCTACCGGAATATAAAGCCACGCGCGCCCAGGCAGGCGATTTTATGTCGCTGTGCAAAAACGCCGAGCTGGCGTGCGAAGt                 <  1:281511/140‑1 (MQ=255)                                                                                                                   ctgcGCCAGCCCGTTGATGCCACTCCAGTATGGATGATGCGCCAGGCGGGTCGCTATCTACCGGAATATAAAGCCACGCGCGCCCAGGCAGGCGATTTTATGTCGCTGTGCAAAAACGCCGAGCTGGCGTGCGAAGTGAc              <  2:148316/140‑1 (MQ=255)                                                                                                                        ccAGCCCGTTGATGCCACTCCAGTATGGATGATGCGCCAGGCGGGTCGCTATCTACCGGAATATAAAGCCACGCGCGCCCAGGCAGGCGATTTTATGTCGCTGTGCAAAAACGCCGAGCTGGCGTGCGAAGTGACTTTGc         <  1:135327/140‑1 (MQ=255)                                                                                                                           gCCCGTTGATGCCACTCCAGTATGGATGATGCGCCAGGCGGGTCGCTATCTACCGGAATATAAAGCCACGCGCGCCCAGGCAGGCGATTTTATGTCGCTGTGCAAAAACGCCGAGCTGGCGTGCGAAGTGACTTTGCAAc      >  1:912787/1‑140 (MQ=255)                                                                                                                             ccGTTGATGCCACTCCAGTATGGATGATGCGCCAGGCGGGTCGCTATCTACCGGAATATAAAGCCACGCGCGCCCAGGCAGGCGATTTTATGTCGCTGTGCAAAAACGCCGAGCTGGCGTGCGAAGTGACTTTGCAACCg    <  2:312975/140‑1 (MQ=255)                                                                                                                               gTTGATGCCACTCCAGTATGGATGATGCGCCAGGCGGGTCGCTATCTACCGGAATATAAAGCCACGCGCGCCCAGGCAGGCGATTTTATGTCGCTGTGCAAAAACGCCGAGCTGGCGTGCGAAGTGACTTTGCAACCGCt  <  1:609666/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                     GATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGTCACTCCAGTATGGATGATGCGCCAGGCGGGTCGCTATCTACCGGAATATAAAGCCACGCGCGCCCAGGCAGGCGATTTTATGTCGCTGTGCAAAAACGCCGAGCTGGCGTGCGAAGTGACTTTGCAACCGCT  >  NC_000913/4197641‑4197906

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑