Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,421,194 T→C S405S (AGT→AGC)  ulaA → L‑ascorbate specific PTS enzyme IIC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,421,1940TC100.0% 107.0 / NA 30S405S (AGT→AGC) ulaAL‑ascorbate specific PTS enzyme IIC component
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/4);  total (26/4)

TGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGATCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCT  >  NC_000913/4421058‑4421328
                                                                                                                                        |                                                                                                                                      
tGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGTGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGATCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGCGcc                                                                                                                                     <  2:868828/140‑1 (MQ=255)
tGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGATCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGCGcc                                                                                                                                     <  2:1346033/140‑1 (MQ=255)
    cttctCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGATCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGCGCCTGGa                                                                                                                                 >  2:397557/1‑140 (MQ=255)
          tAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGATCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGCGCCTGGATGGGCa                                                                                                                           >  1:1079990/1‑140 (MQ=255)
            aCGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGATCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGCGCCTGGATGGGCATg                                                                                                                         >  2:919857/1‑140 (MQ=255)
            aCGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGATCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGCGCCTGGATGGGCATg                                                                                                                         >  1:1210271/1‑140 (MQ=255)
                  ccaTCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGATCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGCGCCTGGATGGGCATGGCGGAc                                                                                                                   >  1:966119/1‑140 (MQ=255)
                      cGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGATCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGCGCCTGGATGGGCATGGCGGACTGGt                                                                                                               <  1:1361184/140‑1 (MQ=255)
                       ggCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGATCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGCGCCTGGATGGGCATGGCGGACTGGTc                                                                                                              <  2:1179896/140‑1 (MQ=255)
                          gtgtTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGATCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGCGCCTGGATGGGCATGGCGGACTGGTCGAt                                                                                                           >  1:1102059/1‑140 (MQ=255)
                               cGCTACCCCTTTGGGGGGCTGGCGTGCGGCGCTGAAGTTTTGTCGGGTGAGGGGGATGATCGAAATCTTTGGTTGCGTGGGGGGGGTAAACCTCACGGGTAGGAGCGCGGGGAGGGGCAGGGCGGACTGGTCGTTTCTgg                                                                                                      <  2:810885/140‑1 (MQ=255)
                                       aCTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGATCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGCGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCAccgccg                                                                                              >  2:770962/1‑140 (MQ=255)
                                       aCTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGATCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGCGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCAccgccg                                                                                              >  1:681939/1‑140 (MQ=255)
                                        cTTCGGGGGCTGGCGTGGGGCGGTGAAGTTTTGTCGGGTGAGGGGGATGATCGAAATTTTTGGTTGCGTCGGGGCGGTGAACCTCACCGGTATGAGCGCCGGGATGGGCAGGGCGGACTGGTCGATTCTGGCACCGCCga                                                                                             <  2:966119/140‑1 (MQ=255)
                                              cggcTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGATCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGCGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGc                                                                                       >  2:857765/1‑140 (MQ=255)
                                                   ggCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGATCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGCGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGc                                                                                  >  1:1122948/1‑140 (MQ=255)
                                                   ggCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGATCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGCGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGc                                                                                  >  1:179686/1‑140 (MQ=255)
                                                   ggCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGATCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGCGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGc                                                                                  >  1:1308004/1‑140 (MQ=255)
                                                   ggCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGATCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGCGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGc                                                                                  >  2:1104346/1‑140 (MQ=255)
                                                      ggggggcgCTGAAGATTTGTCTGGTGAGGGGGATGATCGAAATCTTTGGTTGCGTCGGGGCGGTGAAACTCCCCGGTATGAGCGCCTGGATGGGCATGGCGGACTGGTCGTTTCTGGCACCGCCGATGATGCAAGGcttc                                                                               <  2:1181013/136‑1 (MQ=255)
                                                             gcTGAAGTTTTGTCTGGTGGGGGGGATGATCAAAATTTTTGGTTGCGTGGGGGGGGTGAAACTCCCGGGTATGAGCGCGTGGATGGGCATGGCGGCCTGGTCGTTTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCa                                                                        <  2:624555/140‑1 (MQ=255)
                                                                           tGGTGATGGGGATGATCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGCGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCttt                                                          >  2:44918/1‑140 (MQ=255)
                                                                                ggggggATGATCGAATTTTTTGGTTGCGTCGGGGGGGTAAACCCCCCGGGTATGAGCGCGGGGAGGGGCATGGCGGACTGGTCGATTCTGGCCCCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGc                                                     <  2:1122948/138‑1 (MQ=255)
                                                                                ggggggATGATCGAAATTTTTGGTGGCGTGGGGGGGGTAAACCTCCCCGGTATGGGCGCCTGGAGGGGCAGGGCGGCTTGGTCGTTTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGc                                                     <  2:179686/138‑1 (MQ=255)
                                                                                  ggggACGCTCGAAAGGTTTGGTTGCGCATGGGCGGCGAAACCCACCGAGATGATCGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCg                                                   >  2:1220245/1‑140 (MQ=255)
                                                                                   gggATGATCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGCGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGt                                                  >  2:1072939/1‑140 (MQ=255)
                                                                                   gggATGATCAAAATTTTTGGTTGCGTGGGGGCGGGAAACCTCCCCGGTAGGAGCGCTGGGAGGGGCATGGCGGCCTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGt                                                  <  2:1210271/140‑1 (MQ=255)
                                                                                    ggaaggtCGAAGTCTTGGTTTGGGTGGGGGGGTTAAACCTCCCGGGTATGCGCGCCTGGTGGGGCATGGCGGAGTGTTCTTTTCTGGCTCCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGtc                                                 <  2:736311/134‑1 (MQ=255)
                                                                                     gatgatCAAATTCTTTGGTTGCGTCGGGGGGGTGAACCTCCCCGGTATGAGCGCGTGGATGGGCATGGCGGACTGGTCGTTTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGtca                                                <  2:359776/140‑1 (MQ=255)
                                                                                       tgatCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGCGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGtcatc                                              >  1:1050369/1‑140 (MQ=255)
                                                                                       tgatCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGCGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGtcatc                                              >  1:1166454/1‑140 (MQ=255)
                                                                                       tgatCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGCGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGtcatc                                              >  1:1269701/1‑140 (MQ=255)
                                                                                        gatCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGCGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGtcatca                                             >  2:580008/1‑140 (MQ=255)
                                                                                          tCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGCGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCAtt                                           >  2:839713/1‑140 (MQ=255)
                                                                                              attCTTGGGTTGCGTCTGGGCGGTGAAACTCCCCGGTATGAGCGCCTGGATGGGCAGGGCGGACGGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTaa                                       <  2:1166454/138‑1 (MQ=255)
                                                                                               aTCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGCGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAAt                                      >  1:1183559/1‑140 (MQ=255)
                                                                                               aTCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGCGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAAt                                      >  1:736311/1‑140 (MQ=255)
                                                                                                   ttGGTTGCGTCGGGGGGGTGAACCTCCCGGGTATGAGCGCCTGGATGGGCGTGGCGGACTGGTCGTTTCGGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCa                                  <  2:404426/140‑1 (MQ=255)
                                                                                                   ttGGTTCCGTCTGGGGGGTAAACCCCACCGGTATGAGCGCTTGGAGGGGCAGGGCGGTGTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCa                                  <  2:28725/140‑1 (MQ=255)
                                                                                                    tGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGCGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCAc                                 >  2:162598/1‑140 (MQ=255)
                                                                                                    tGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGCGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCAc                                 <  1:839713/140‑1 (MQ=255)
                                                                                                    tGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGCGCCTGGATGGGCAGGGCGGACTGGTCGTTTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCAc                                 <  2:1079990/140‑1 (MQ=255)
                                                                                                          cGTCTGGGCGGTGAAACTCACCGGTATGAGCGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCtt                           >  1:478236/1‑140 (MQ=255)
                                                                                                            tCTGGGCGGTGAAACTCACCGGTATGAGCGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTtat                         >  1:1381261/1‑140 (MQ=255)
                                                                                                                    gTGAAACTCACCGGTATGAGCGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGttctt                 >  2:17516/1‑140 (MQ=255)
                                                                                                                                   aTGAGCGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCt  <  1:1072939/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                      
TGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGATCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCT  >  NC_000913/4421058‑4421328

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑