Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,425,689	A→G	H57R (CAC→CGC)	priB →	primosomal replication protein N

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,425,689	0	A	G	100.0%	272.6 / NA	77	H57R (CAC→CGC)	priB	primosomal replication protein N
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (38/39);  total (38/39)

TGCAGGGCTCCCCTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGACACGAAAACCAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATT  >  NC_000913/4425553‑4425823                                                                                                                                         |                                                                                                                                       tgCAGGGCTCCCCTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGACGCGa                                                                                                                                     >  1:443182/1‑140 (MQ=255)    aGGGCTCCCCTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGACGCGaaaa                                                                                                                                  >  1:471725/1‑140 (MQ=255)       gCTCCCCTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGACGCGAAAACCa                                                                                                                               >  1:108275/1‑140 (MQ=255)         tCCCCTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGACGCGAAAACCAgg                                                                                                                             <  2:208441/140‑1 (MQ=255)         tCCCCTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGACGCGAAAACCAgg                                                                                                                             <  2:443182/140‑1 (MQ=255)           ctcTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCCCCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGACGCGAAAACCAGGcc                                                                                                                           <  2:193414/138‑1 (MQ=255)           cccTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGACGCGAAAACCAgg                                                                                                                             <  1:490961/138‑1 (MQ=255)           cccTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGACGCGAAAACCAgg                                                                                                                             >  2:490961/1‑138 (MQ=255)           cccTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGACGCGAAAACCAGGcc                                                                                                                           >  1:1120324/1‑140 (MQ=255)           cccTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGACGCGAAAACCAGGcc                                                                                                                           >  2:1386602/1‑140 (MQ=255)           cccTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGACGCGAAAACCAGGcc                                                                                                                           >  1:509386/1‑140 (MQ=255)           cccTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGACGCGAAAACCAGGcc                                                                                                                           >  2:841292/1‑140 (MQ=255)           cccTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGACGCGAAAACCAGGcc                                                                                                                           >  1:206891/1‑140 (MQ=255)           cccTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGACGCGAAAACCAGGcc                                                                                                                           >  1:275246/1‑140 (MQ=255)            ccTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGACGCGAAAACCAGGCCa                                                                                                                          <  1:1063797/140‑1 (MQ=255)              ttCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGACGCGAAAACCAGGCCAtt                                                                                                                        <  2:980402/140‑1 (MQ=255)                cGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGACGCGAAAACCAGGCCATTAc                                                                                                                      >  1:1258091/1‑140 (MQ=255)                 gAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGACGCGAAAACCAGGCCATTACt                                                                                                                     <  1:750691/140‑1 (MQ=255)                 gAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGACGCGAAAACCAGGCCATTACt                                                                                                                     <  1:323883/140‑1 (MQ=255)                 gAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGACGCGAAAACCAGGCCATTACt                                                                                                                     <  2:1298391/140‑1 (MQ=255)                 gAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGACGCGAAAACCAGGCCATTACt                                                                                                                     <  2:1214733/140‑1 (MQ=255)                 gAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGACGCGAAAACCAGGCCATTACt                                                                                                                     <  2:118201/140‑1 (MQ=255)                  aaaGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGACGCGAAAACCAGGCCATTACt                                                                                                                     >  1:142285/1‑139 (MQ=255)                  aaaGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGACGCGAAAACCAGGCCATTACt                                                                                                                     <  2:142285/139‑1 (MQ=255)                   aaGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGACGCGAAAACCAGGCCATTACTca                                                                                                                   <  2:1120324/140‑1 (MQ=255)                   aaGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGACGCGAAAACCAGGCCATTACTca                                                                                                                   <  1:1386602/140‑1 (MQ=255)                     ggTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGACGCGAAAACCAGGCCATTACTcaca                                                                                                                 >  1:999881/1‑140 (MQ=255)                     ggTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTAACCGGCAGTCGTGGTGTCAAATGCCCGTTATTGTTAGCGGACGCGAAAAACAAGCCATTAATcaca                                                                                                                 >  2:1275945/1‑140 (MQ=255)                      gTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGACGCGAAAACCAGGCCATTACTCACAg                                                                                                                >  2:417362/1‑140 (MQ=255)                         aGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGACGCGAAAACCAGGCCATTACTCACAGtat                                                                                                             >  2:450561/1‑140 (MQ=255)                           tCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGACGCGAAAACCAGGCCATTACTCACAGTATaa                                                                                                           <  2:206891/140‑1 (MQ=255)                               tCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGACGCGAAAACCAGGCCATTACTCACAGTATAACGGt                                                                                                       >  2:1152779/1‑140 (MQ=255)                                   gAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGACGCGAAAACCAGGCCATTACTCACAGTATAACGGTCGGc                                                                                                   <  1:1152779/140‑1 (MQ=255)                                     aTTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGACGCGAAAACCAGGCCATTACTCACAGTATAACGGTCGGCAg                                                                                                 <  2:108275/140‑1 (MQ=255)                                         cTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGACGCGAAAACCAGGCCATTACTCACAGTATAACGGTCGGCAGTCGc                                                                                             >  2:906394/1‑140 (MQ=255)                                           cACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGACGCGAAAACCAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCAt                                                                                           <  2:1067755/140‑1 (MQ=255)                                           cACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGACGCGAAAACCAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCAt                                                                                           <  2:509205/140‑1 (MQ=255)                                           cACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGACGCGAAAACCAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCAt                                                                                           <  1:841292/140‑1 (MQ=255)                                             cTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGACGCGAAAACCAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATaa                                                                                         <  1:688873/140‑1 (MQ=255)                                              tGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTTAAATGCCCGTTATTGTTAGCGGACGCGAAAACCAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAAc                                                                                        >  1:1329863/1‑140 (MQ=255)                                                ccAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGACGCGAAAACCAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCg                                                                                      >  2:457006/1‑140 (MQ=255)                                                   gTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGACGCGAAAACCAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTc                                                                                   >  1:703197/1‑140 (MQ=255)                                                   gTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGACGCGAAAACCAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTc                                                                                   >  1:676855/1‑140 (MQ=255)                                                     tCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGACGCGAAAACCAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAg                                                                                 >  2:882740/1‑140 (MQ=255)                                                     tCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGACGCGAAAACCAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAg                                                                                 <  1:1267149/140‑1 (MQ=255)                                                        tGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGACGCGAAAACCAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAgggg                                                                              >  1:1027282/1‑140 (MQ=255)                                                         gCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGACGCGAAAACCAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGt                                                                             <  2:703197/140‑1 (MQ=255)                                                              aGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGACGCGAAAACCAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGttcatt                                                                        >  1:84577/1‑140 (MQ=255)                                                                   cGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGACGCGAAAACCAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATg                                                                   >  1:1107767/1‑140 (MQ=255)                                                                   cGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGACGCGAAAACCAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATg                                                                   >  1:334258/1‑140 (MQ=255)                                                                      tCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGACGCGAAAACCAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCca                                                                <  2:509386/140‑1 (MQ=255)                                                                      tCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGACGCGAAAACCAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCca                                                                <  2:334258/140‑1 (MQ=255)                                                                      tCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGACGCGAAAACCAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCca                                                                <  2:275246/140‑1 (MQ=255)                                                                       cTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGACGCGAAAACCAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCcac                                                               <  2:999881/140‑1 (MQ=255)                                                                          tgCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGACGCGAAAACCAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTACATTTCATGCCACAAg                                                            >  1:176877/1‑140 (MQ=255)                                                                            cAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGACGCGAAAACCAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGc                                                          <  2:1027282/140‑1 (MQ=255)                                                                             aggaggAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGACGCGAAAACCAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCa                                                         >  2:307853/1‑140 (MQ=255)                                                                              ggaggaAGCCGGCTTTCATCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGACGCGAAAACCAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCaa                                                        <  2:623628/140‑1 (MQ=255)                                                                               gaggaAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGACGCGAAAACCAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCaaa                                                       >  2:724881/1‑140 (MQ=255)                                                                                    aGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGACGCGAAAACCAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACg                                                  >  1:725255/1‑140 (MQ=255)                                                                                    aGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGACGCGAAAACCAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACg                                                  >  2:1005070/1‑140 (MQ=255)                                                                                    aGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGACGCGAAAACCAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACg                                                  >  1:321260/1‑140 (MQ=255)                                                                                        ggCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGACGCGAAAACCAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACt                                              <  1:906394/140‑1 (MQ=255)                                                                                         gCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGACGCGAAAACCAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTg                                             <  2:1107767/140‑1 (MQ=255)                                                                                           tttCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGACGCGAAAACCAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAg                                           <  1:943664/140‑1 (MQ=255)                                                                                              cACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGACGCGAAAACCAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCaa                                        <  1:450561/140‑1 (MQ=255)                                                                                                    cAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGACGCGAAAACCAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGt                                  >  1:1079070/1‑140 (MQ=255)                                                                                                        cGTGGTGTCAAATGCCCGTTATTGTTAGCGGACGCGAAAACCAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTTATGCCACTAGGCAAAGAACGGACTGAGCAAAATGTTTTTg                              >  2:1236270/1‑140 (MQ=255)                                                                                                            gtgtCAAATGCCCGTTATTGTTAGCGGACGCGAAAACCAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATg                          <  1:457006/140‑1 (MQ=255)                                                                                                                        cGTTATTGTTAGCGGACGCGAAAACCAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGat                <  1:964875/138‑1 (MQ=255)                                                                                                                        cGTTATTGTTAGCGGACGCGAAAACCAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGat                >  2:964875/1‑138 (MQ=255)                                                                                                                         gTTATTGTTAGCGGACGCGAAAACCAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGattga             <  1:1005173/140‑1 (MQ=255)                                                                                                                          ttATTGTTAGCGGACGCGAAAACCAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGattgaa            <  2:1329863/140‑1 (MQ=255)                                                                                                                               gTTAGCGGACGCGAAAACCAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGAt       <  1:283117/140‑1 (MQ=255)                                                                                                                               gTTAGCGGACGCGAAAACCAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGAt       <  1:307853/140‑1 (MQ=255)                                                                                                                               gTTAGCGGACGCGAAAACCAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGAt       <  2:828947/140‑1 (MQ=255)                                                                                                                               gTTAGCGGACGCGAAAACCAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGAt       >  2:283117/1‑140 (MQ=255)                                                                                                                                   gCGGACGCGAAAACCAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGAt   >  1:734005/1‑140 (MQ=255)                                                                                                                                    cGGACGCGAAAACCAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGAtt  >  1:341847/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       TGCAGGGCTCCCCTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGACACGAAAACCAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATT  >  NC_000913/4425553‑4425823

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑