Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,446,443 C→T R778C (CGT→TGT)  tamB → translocation and assembly module subunit TamB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,446,4430CT98.0% 164.1 / NA 51R778C (CGT→TGT) tamBtranslocation and assembly module subunit TamB
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (29/21);  total (30/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTGGGCTGGACCATCCGCCTGACCAATAACGGCCA  >  NC_000913/4446306‑4446576
                                                                                                                                         |                                                                                                                                     
aCCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGgtgt                                                                                                                                     >  2:75534/1‑140 (MQ=255)
    cTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGTGTAACg                                                                                                                                 >  2:224525/1‑140 (MQ=255)
     ttCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGCATCTTCACGGGTAAAGCGAACGTTCCCTGGGACACCACGAAAGAGGGGCTGCCGCAGCGCAGCATCACCCTTTCGGGGCGTAACga                                                                                                                                >  2:626720/1‑139 (MQ=255)
      tCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGTGTAACGTg                                                                                                                               <  1:682934/140‑1 (MQ=255)
                                 tGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATgcg                                                                                                    >  2:1080975/1‑140 (MQ=255)
                                    cAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCGCGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGTGTAACGTGCAGGTAACGCAAACGGTCAACGATgcggcg                                                                                                 <  1:626720/140‑1 (MQ=255)
                                     aGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGTGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGgcgc                                                                                                >  2:622165/1‑140 (MQ=255)
                                       aaaCCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGTGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTg                                                                                              >  2:1388059/1‑140 (MQ=255)
                                             cTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGTGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTg                                                                                        >  1:1019930/1‑140 (MQ=255)
                                             cTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGTGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTg                                                                                        >  1:560676/1‑140 (MQ=255)
                                                aGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGTGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCg                                                                                     >  1:83618/1‑140 (MQ=255)
                                                  gCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGTGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCg                                                                                          <  2:938916/133‑1 (MQ=255)
                                                  gCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGTGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCg                                                                                          >  1:938916/1‑133 (MQ=255)
                                                  gCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGTGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGtt                                                                                   >  1:27719/1‑140 (MQ=255)
                                                   ccAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGTGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGttt                                                                                  <  1:1147986/140‑1 (MQ=255)
                                                   ccAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGTGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGttt                                                                                  <  1:622165/140‑1 (MQ=255)
                                                     aGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGTGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCa                                                                                <  1:1013811/140‑1 (MQ=255)
                                                        ggTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGTGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGac                                                                             >  2:466502/1‑140 (MQ=255)
                                                         gTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGTGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGaca                                                                            <  2:27719/140‑1 (MQ=255)
                                                          tATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGTGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGacac                                                                           >  1:1105918/1‑140 (MQ=255)
                                                                 aCGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGTGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCt                                                                    >  2:135429/1‑140 (MQ=255)
                                                                    ggTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGTGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGAc                                                                 <  1:1192852/140‑1 (MQ=255)
                                                                      tAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGTGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGAcgg                                                               >  1:89318/1‑140 (MQ=255)
                                                                        aaGCGGACGTTGCCTGGGACACCACGAAAGAGGGGTTGCCGCAGGGCAGTATCACCCTTTCGGGGTGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGAcggcg                                                             >  2:305673/1‑140 (MQ=255)
                                                                         aGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGTGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGAcggcgg                                                            <  1:1093398/140‑1 (MQ=255)
                                                                          gCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGTGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGa                                                           >  2:869426/1‑140 (MQ=255)
                                                                              aCGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGTGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTg                                                       <  2:1019930/140‑1 (MQ=255)
                                                                               cGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGTGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGc                                                      >  2:116688/1‑140 (MQ=255)
                                                                                 ttGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGTGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGt                                                    <  1:624115/140‑1 (MQ=255)
                                                                                   gCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGTGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTaa                                                  >  1:685398/1‑140 (MQ=255)
                                                                                     cTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGTGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTaaca                                                >  1:337974/1‑140 (MQ=255)
                                                                                          acacCACGAAAGGGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGTGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGt                                           >  1:903383/1‑140 (MQ=255)
                                                                                           caccacGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGTGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTg                                          <  1:75534/140‑1 (MQ=255)
                                                                                              cacGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGTGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCg                                       >  2:424125/1‑140 (MQ=255)
                                                                                              cacGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGTGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCg                                       >  2:1050817/1‑140 (MQ=255)
                                                                                                cGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGTGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGaa                                     <  1:517558/140‑1 (MQ=255)
                                                                                                cGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGTGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGaa                                     <  2:685398/140‑1 (MQ=255)
                                                                                                 gAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGTGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAAt                                    <  1:466502/140‑1 (MQ=255)
                                                                                                 gAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGTGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAAt                                    >  2:714268/1‑140 (MQ=255)
                                                                                                   aaGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGTGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTg                                  >  1:1316078/1‑140 (MQ=255)
                                                                                                     gagGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGTGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTggg                                <  1:224525/140‑1 (MQ=255)
                                                                                                        gggCTGCCGCAGGGCAGTATCACCCTTTCGGGGTGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTGGGCTg                             <  1:714268/140‑1 (MQ=255)
                                                                                                        gggCTGCCGCAGGGCAGTATCACCCTTTCGGGGTGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTGGGCTg                             >  1:691618/1‑140 (MQ=255)
                                                                                                        gggCTGCCGCAGGGCAGTATCACCCTTTCGGGGTGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTGGGCTg                             <  2:560676/140‑1 (MQ=255)
                                                                                                                              cccTTTCGGGGTGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGTGGAATTGCGTAACAACCGTGCCGAATTGGGCTGGACCATCCGCCTGACCAATAAc       >  1:1101395/1‑140 (MQ=255)
                                                                                                                              cccTTTCGGGGTGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTGGGCTGGACCATCCGCCTGACCAATAAc       >  1:624717/1‑140 (MQ=255)
                                                                                                                               ccTTTCGGGGTGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTGGGCTGGACCATCCGCCTGACCAATAACg      >  1:805205/1‑140 (MQ=255)
                                                                                                                                 tttCGGGGTGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTGGGCTGGACCATCCGCCTGACCAATAACGGc    >  2:828278/1‑140 (MQ=255)
                                                                                                                                   tCGGGGTGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTGGGCTGGACCATCCGCCt                 >  1:1043587/1‑125 (MQ=255)
                                                                                                                                   tCGGGGTGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTGGGCTGGACCATCCGCCt                 <  2:1043587/125‑1 (MQ=255)
                                                                                                                                   tCGGGGTGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTGGGCTGGACCATCCGCCTGACCAATAACGGc    >  2:1397398/1‑138 (MQ=255)
                                                                                                                                   tCGGGGTGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTGGGCTGGACCATCCGCCTGACCAATAACGGc    <  1:1397398/138‑1 (MQ=255)
                                                                                                                                   tCGGGGTGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTGGGCTGGACCATCCGCCTGACCAATAACGGCCa  <  1:305673/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                     
ACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTGGGCTGGACCATCCGCCTGACCAATAACGGCCA  >  NC_000913/4446306‑4446576

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑