Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 531,195 C→T R11H (CGT→CAT)  selU ← tRNA 2‑selenouridine synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913531,1950CT100.0% 142.3 / NA 41R11H (CGT→CAT) selUtRNA 2‑selenouridine synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/21);  total (20/21)

TAGCAGGTGCCAACGGCGGCGCGTTCATCGTTATTCATTAACGGCAGATTGATAGCGGCGGGCATTGCGCCGTGCTCAAACTCGATAGGGGCGCGAACATCAATAATGGGCGTATCAGCAATCAGCAGGGCACGATAGTCCTGTTCCGTGTGTCTCTCTTGCATAGTTAAAAGTGAACCTCAAATCAGCTTGCGCGCTATTTTACGCGCCAACGCGCAAGGAAACTTGATTTTTAACTGCGTGGGTTGCCGAAAATTTCTAAAAATCC  >  NC_000913/531063‑531330
                                                                                                                                    |                                                                                                                                       
taGCAGGTGCCAACGGCGGCGCGTTCATCGTTATTCATTAACGGCAGATTGATAGCGGCGGGCATTGCGCCGTGCTCAAACTCGATAGGGGCGCGAACATCAATAATGGGCGTATCAGCAATCAGCAGGGCATGATAGTc                                                                                                                                  <  1:616775/140‑1 (MQ=255)
     ggTGCCAACGGCGGCGCGTTCATCGTTATTCATTAACGGCAGATTGATAGCGGCGGGCATTGCGCCGTGCTCAAACTCGATAGGGGCGCGAACATCAATAATGGGCGTATCAGCAATCAGCAGGGCATGATAGTCCTGtt                                                                                                                             <  1:21313/140‑1 (MQ=255)
      gTGCCAACGGCGGCGCGTTCATCGTTATTCATTAACGGCAGATTGATAGCGGCGGGGATTGGGCCGGGCTCAAAATCGATAGGGGGGCGAACAACAAAAAAAGGGGGATCCGCAAAAAGCAGGGGCAGATAGTccccgtc                                                                                                                            >  2:591988/1‑135 (MQ=255)
             cggcggCGCGTTCATCGTTATTCATTAACGGCAGATTGATAGCGGCGGGCATTGCGCCGTGCTCAAACTCGATAGGGGCGCGAACATCAATAATGGGCGTATCAGCAATCAGCAGGGCATGATAGTCCTGTTCCgtgtgt                                                                                                                     <  2:905302/140‑1 (MQ=255)
             cggcggCGCGTTCATCGTTATTCATTAACGGCAGATTGATAGCGGCGGGCATTGCGCCGTGCTCAAACTCGATAGGGGCGCGAACATCAATAATGGGCGTATCAGCAATCAGCAGGGCATGATAGTCCTGTTCCgtgtgt                                                                                                                     <  1:419713/140‑1 (MQ=255)
                cggcgCGTTCATCGTTATTCATTAACGGCAGATTGATAGCGGCGGGCATTGCGCCGTGCTCAAACTCGATAGGGGCGCGAACATCAATAATGGGCGTATCAGCAATCAGCAGGGCATGATAGTCCTGTTCCGTGTGtctc                                                                                                                  >  1:498348/1‑140 (MQ=255)
                cggcgCGTTCATCGTTATTCATTAACGGCAGATTGATAGCGGCGGGCATTGCGCCGTGCTCAAACTCCATAGGGGCGCGGACATCAATAAATGGCGTATCAGCAAACAGCAGGGCATGATAGGCCTGTTTCGTGTGtctt                                                                                                                  >  2:214875/1‑139 (MQ=255)
                      gTTCATCGTTATTCATTAACGGCAGATTGATAGCGGCGGGCATTGCGCCGTGCTCAAACTCGATAGGGGCGCGAACATCAATAATGGGCGTATCAGCAATCAGCAGGGCATGATAGTCCTGTTCCGTGTGTCTCTCTTGc                                                                                                            <  1:929917/140‑1 (MQ=255)
                      gTTCATCGTTATTCATTAACGGCAGATTGATAGCGGCGGGCATTGCGCCGTGCTCAAACTCGATAGGGGCGCGAACATCAATAATGGGCGTATCAGCAATCAGCAGGGCATGATAGTCCTGTTCCGTGTGTCTCTCTTGc                                                                                                            <  1:138311/140‑1 (MQ=255)
                       ttCATCGTTATTCATTAACGGCAGATTGATAGCGGCGGGCATTGCGCCGTGCTCAAACTCGATAGGGGCGCGAACATCAATAATGGGCGTATCAGCAATCAGCAGGGCATGATAGTCCTGTTCCGTGTGTCTCTCTTGCa                                                                                                           <  1:937167/140‑1 (MQ=255)
                                   cATTAACGGCAGATTGATAGCGGCGGGCATTGCGCCGTGCTCAAACTCGATAGGGGCGCGAACATCAATAATGGGCGTATCAGCAATCAGCAGGGCATGATAGTCCTGTTCCGTGTGTCTCTCTTGCATAGTTAAAAGTg                                                                                               <  2:1028109/140‑1 (MQ=255)
                                      tAACGGCAGATTGATAGCGGCGGGCATTGCGCCGTGCTCAAACTCGATAGGGGCGCGAACATCAATAATGGGCGTATCAGCAATCAGCAGGGCATGATAGTCCTGTTCCGTGTGTCTCTCTTGCATAGTTAAAAGTGAAc                                                                                            <  1:18942/140‑1 (MQ=255)
                                      tAACGGCAGATTGATAGCGGCGGGCATTGCGCCGTGCTCAAACTCGATAGGGGCGCGAACATCAATAATGGGCGTATCAGCAATCAGCAGGGCATGATAGTCCTGTTCCGTGTGTCTCTCTTGCATAGTTAAAAGTGAAc                                                                                            >  2:255807/1‑140 (MQ=255)
                                       aaCGGCAGATTGATAGCGGCGGGCATTGCGCCGTGCTCAAACTCGATAGGGGCGCGAACATCAATAATGGGCGTATCAGCAATCAGCAGGGCATGATAGTCCTGTTCCGTGTGTCTCTCTTGCATAGTTAAAAGTGAAcc                                                                                           <  2:156369/140‑1 (MQ=255)
                                            cAGATTGATAGCGGCGGGCATTGCGCCGTGCTCAAACTCGATAGGGGCGCGAACATCAATAATGGGCGTATCAGCAATCAGCAGGGCATGATAGTCCTGTTCCGTGTGTCTCTCTTGCATAGTTAAAAGTGAACCTCaaa                                                                                      <  1:113706/140‑1 (MQ=255)
                                               aTTGATAGCGGCGGGCATTGCGCCGTGCTCAAACTCGATAGGGGCGCGAACATCAATAATGGGCGTATCAGCAATCAGCAGGGCATGATAGTCCTGTTCCGTGTGTCTCTCTTGCATAGTTAAAAGTGAACCTCAAATCa                                                                                   >  1:498125/1‑140 (MQ=255)
                                                   aTAGCGGCGGGCATTGCGCCGTGCTCAAACTCGATAGGGGCGCGAACATCAATAATGGGCGTATCAGCAATCAGCAGGGCATGATAGTCCTGTTCCGTGTGTCTCTCTTGCATAGTTAAAAGTGAACCTCAAATCAGCtt                                                                               <  1:255807/140‑1 (MQ=255)
                                                         gcggGCATTGCGCCGTGCTCAAACTCGATAGGGGCGCGAACATCAATAATGGGCGTATCAGCAATCAGCAGGGCATGATAGTCCTGTTCCGTGTGTCTCTCTTGCATAGTTAAAAGTGAACCTCAAATCAGCTTgc                                                                             >  1:294912/1‑136 (MQ=255)
                                                         gcggGCATTGCGCCGTGCTCAAACTCGATAGGGGCGCGAACATCAATAATGGGCGTATCAGCAATCAGCAGGGCATGATAGTCCTGTTCCGTGTGTCTCTCTTGCATAGTTAAAAGTGAACCTCAAATCAGCTTgc                                                                             <  2:294912/136‑1 (MQ=255)
                                                            ggCATTGCGCCGTGCTCAAACTCGACAGGGGCGCGAACATCAATAATGGGCGTATCAGCAATCAGCAGGGCATGATAGTCCTGTTCCGTGTGTCTCTCTTGCATAGTTAAAAGTGAACCTCAAATCAGCTTGCGCGCTAt                                                                      >  1:1270660/1‑140 (MQ=255)
                                                                       gTGCTCAAACTCGATAGGGGCGCGAACATCAATAATGGGCGTATCAGCAATCAGCAGGGCATGATAGTCCTGTTCCGTGTGTCTCTCTTGCATAGTTAAAAGTGAACCTCAAATCAGCTTgcgc                                                                           >  2:1048215/1‑124 (MQ=255)
                                                                       gTGCTCAAACTCGATAGGGGCGCGAACATCAATAATGGGCGTATCAGCAATCAGCAGGGCATGATAGTCCTGTTCCGTGTGTCTCTCTTGCATAGTTAAAAGTGAACCTCAAATCAGCTTgcgc                                                                           <  1:1048215/124‑1 (MQ=255)
                                                                       gTGCTCAAACTCGATAGGGGCGCGAACATCAATAATGGGCGTATCAGCAATCAGCAGGGCATGATAGTCCTGTTCCGTGTGTCTCTCTTGCATAGTTAAAAGTGAACCTCAAATCAGCTTGCGCGCTATTTTACGCGCCa                                                           <  1:435999/140‑1 (MQ=255)
                                                                          cTCAAACTCGATAGGGGCGCGAACATCAATAATGGGCGTATCAGCAATCAGCAGGGCATGATAGTCCTGTTCCGTGTGTCTCTCTTGCATAGTTAAAAGTGAACCTCAAATCAGCTTGCGCGCTATTTTACGCGCCAAcg                                                        <  1:214875/140‑1 (MQ=255)
                                                                                  cGATAGGGGCGCGAACATCAATAATGGGCGTATTAGCAATCAGCAGGGCATGATAGTCCTGTTCCGTGTGTCTCTCTTGCATAGTTAAAAGTGAACCTCAAATCAGCTTGCGCGCTATTTTACGCGCCAACGCGCAAGGa                                                >  1:1072472/1‑140 (MQ=255)
                                                                                  cGATAGGGGCGCGAACATCAATAATGGGCGTATCAGCAATCAGCAGGGCATGATAGTCCTGTTCCGTGTGTCTCTCTTGCATAGTTAAAAGTGAACCTCAAATCAGCTTGCGCGCTATTTTACGCGCCAACGCGCAAGGa                                                >  1:643907/1‑140 (MQ=255)
                                                                                  cGATAGGGGCGCGAACATCAATAATGGGCGTATCAGCAATCAGCAGGGCATGATAGTCCTGTTCCGTGTGTCTCTCTTGCATAGTTAAAAGTGAACCTCAAATCAGCTTGCGCGCTATTTTACGCGCCAACGCGCAAGGa                                                <  2:498348/140‑1 (MQ=255)
                                                                                    aTAGGGGCGCGAACATCAATAATGGGCGTATCAGCAATCAGCAGGGCATGATAGTCCTGTTCCGTGTGTCTCTCTTGCATAGTTAAAAGTGAACCTCAAATCAGCTTGCGCGCTATTTTACGCGCCAACGCGCAAGGaaa                                              <  1:354767/140‑1 (MQ=255)
                                                                                    aTAGGGGCGCGAACATCAATAATGGGCGTATCAGCAATCAGCAGGGCATGATAGTCCTGTTCCGTGTGTCTCTCTTGCATAGTTAAAAGTGAACCTCAAATCAGCTTGCGCGCTATTTTACGCGCCAACGCGCAAGGaaa                                              >  1:1395758/1‑140 (MQ=255)
                                                                                      aGGGGCGCGAACATCAATAATGGGCGTATCAGCAATCAGCAGGGCATGATAGTCCTGTTCCGTGTGTCTCTCTTGCATAGTTAAAAGTGAACCTCAAATCAGCTTGCGCGCTATTTTACGCGCCAACGCGCAAGGAAACt                                            <  2:1395758/140‑1 (MQ=255)
                                                                                      aGGGGCGCGAACATCAATAATGGGCGTATCAGCAATCAGCAGGGCATGATAGTCCTGTTCCGTGTGTCTCTCTTGCATAGTTAAAAGTGAACCTCAAATCAGCTTGCGCGCTATTTTACGCGCCAACGCGCAAGGAAACt                                            <  1:296261/140‑1 (MQ=255)
                                                                                           cgcgAACATCAATAATGGGCGTATCAGCAATCAGCAGGGCATGATAGTCCTGTTCCGTGTGTCTCTCTTGCATAGTTAAAAGTGAACCTCAAATCAGCTTGCGCGCTATTTTACGCGCCAACGCGCAAGGAAACTTGAtt                                       >  1:1161191/1‑140 (MQ=255)
                                                                                                            ggCGTATCAGCAATCAGCAGGGCATGATAGTCCTGTTCCGTGTGTCTCTCTTGCATAGTTAAAAGTGAACCTCAAATCAGCTTGCGCGCTATTTTACGCGCCAACGCGCAAGGAAACTTGATTTTTAACTGCGTGGGTTg                      >  1:1076130/1‑140 (MQ=255)
                                                                                                              cGTATCAGCAATCAGCAGGGCATGATAGTCCTGTTCCGTGTGTCTCTCTTGCATAGTTAAAAGTGAACCTCAAATCAGCTTGCGCGCTATTTTACGCGCCAACGCGCAAGGAAACTTGATTTTTaa                                  >  1:456818/1‑126 (MQ=255)
                                                                                                              cGTATCAGCAATCAGCAGGGCATGATAGTCCTGTTCCGTGTGTCTCTCTTGCATAGTTAAAAGTGAACCTCAAATCAGCTTGCGCGCTATTTTACGCGCCAACGCGCAAGGAAACTTGATTTTTaa                                  <  2:456818/126‑1 (MQ=255)
                                                                                                              cGTATCAGCAATCAGCAGGGCATGATAGTCCTGTTCCGTGTGTCTCTCTTGCATAGTTAAAAGTGAACCTCAAATCAGCTTGCGCGCTATTTTACGCGCCAACGCGCAAGGAAACTTGATTTTTAACTGCGTGGGTTGcc                    >  1:1373446/1‑140 (MQ=255)
                                                                                                                 atcagcaatcagcaGGGCATGATAGTCCTGTTCCGTGTGTCTCTCTTGCATAGTTAAAAGTGAACCTCAAATCAGCTTGCGCGCTATTTTACGCGCCAACGCGCAAGGAAACTTGATTTTTAACTGCGTGGGTTGCCGaa                 >  1:286062/1‑140 (MQ=255)
                                                                                                                  tcagcaatcagcaGGGCATGATAGTCCTGTTCCGTGTGTCTCTCTTGCATAGTTAAAAGTGAACCTCAAATCAGCTTGCGCGCTATTTTACGCGCCAACGCGCAAGGAAACTTGATTTTTAACTGCGTGGGTTGCCGaaa                >  1:1251015/1‑140 (MQ=255)
                                                                                                                      caatcagcaGGGCATGATAGTCCTGTTCCGTGTGTCTCTCTTGCATAGTTAAAAGTGAACCTCAAATCAGCTTGCGCGCTATTTTACGCGCCAACGCGCAAGGAAACTTGATTTTTAACTGCGTGGGTTGCCGAAAAttt            >  2:1310270/1‑140 (MQ=255)
                                                                                                                      caatcagcaGGGCATGATAGTCCTGTTCCGTGTGTCTCTCTTGCATAGTTAAAAGTGAACCTCAAATCAGCTTGCGCGCTATTTTACGCGCCAACGCGCAAGGAAACTTGATTTTTAACTGCGTGGGTTGCCGAAAAttt            >  2:676871/1‑140 (MQ=255)
                                                                                                                        atcagcaGGGCATGATAGTCCTGTTCCGTGTGTCTCTCTTGCATAGTTAAAAGTGAACCTCAAATCAGCTTGCGCGCTATTTTACGCGCCAACGCGCAAGGAAACTTGATTTTTAACTGCGTGGGTTGCCGAAAATTTCt          >  2:220185/1‑140 (MQ=255)
                                                                                                                             cagGGCATGATAGTCCTGTTCCGTGTGTCTCTCTTGCATAGTTAAAAGTGAACCTCAAATCAGCTTGCGCGCTATTTTACGCGCCAACGCGCAAGGAAACTTGATTTTTAACTGCGTGGGTTGCCGAAAATTTCTaaaaa     >  2:440228/1‑140 (MQ=255)
                                                                                                                              agGGCATGATAGTCCTGTTCCGTGTGTCTCTCTTGCATAGTTAAAAGTGAACCTCAAATCAGCTTGCGCGCTATTTTACGCGCCAACGCGCAAGGAAACTTGATTTTTAACTGCGTGGGTTGCCGAAAATTTCTAAAAAt    >  1:575082/1‑140 (MQ=255)
                                                                                                                                ggCATGATAGTCCTGTTCCGTGTGTCTCTCTTGCATAGTTAAAAGTGAACCTCAAATCAGCTTGCGCGCTATTTTACGCGCCAACGCGCAAGGAAACTTGATTTTTAACTGCGTGGGTTGCCGAAAATTTCTAAAAATcc  >  2:689538/1‑140 (MQ=255)
                                                                                                                                    |                                                                                                                                       
TAGCAGGTGCCAACGGCGGCGCGTTCATCGTTATTCATTAACGGCAGATTGATAGCGGCGGGCATTGCGCCGTGCTCAAACTCGATAGGGGCGCGAACATCAATAATGGGCGTATCAGCAATCAGCAGGGCACGATAGTCCTGTTCCGTGTGTCTCTCTTGCATAGTTAAAAGTGAACCTCAAATCAGCTTGCGCGCTATTTTACGCGCCAACGCGCAAGGAAACTTGATTTTTAACTGCGTGGGTTGCCGAAAATTTCTAAAAATCC  >  NC_000913/531063‑531330

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑