Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	581,060	A→T	D76V (GAT→GTT)	nohD →	DLP12 prophage; putative DNA‑packaging protein NohD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	581,060	0	A	T	100.0%	163.4 / NA	53	D76V (GAT→GTT)	nohD	DLP12 prophage; putative DNA‑packaging protein NohD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (24/29);  total (24/29)

CGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCTGCAGGCCAGCGAGACAGATCTCCAGCCAGGGACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGATGCACAGGAGCTGAAAAATGCCAGAGACTCCGCTGAAGTGGTGGAAACCGCATTCTGTACTTTCGTGCTGTCGCGGATC  >  NC_000913/580924‑581196                                                                                                                                         |                                                                                                                                         cGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCTGCAGGCCAGCGAGACAGTtct                                                                                                                                       >  2:23026/1‑140 (MQ=255)  gAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCTGCAGGCCAGCGAGACAGTtctc                                                                                                                                      <  2:1045678/140‑1 (MQ=255)  gAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTCAAGAACTGCTGCAGGCCAGCGAGACAGTtctc                                                                                                                                      <  2:951673/140‑1 (MQ=255)      cGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCTGCAGGCCAGCGAGACAGTTCTccagc                                                                                                                                  >  2:358762/1‑140 (MQ=255)       ggTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCTGCAGGCCAGCGAGACAGTTCTccagcc                                                                                                                                 <  2:186085/140‑1 (MQ=255)          ggCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCTGCAGGCCAGCGAGACAGTTCTCCAGCCAgg                                                                                                                              <  1:345388/140‑1 (MQ=255)            cAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCTGCAGGCCAGCGAGACAGTTCTCCAGCCAGGGa                                                                                                                            <  2:1140431/140‑1 (MQ=255)               gggTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCTGCAGGCCAGCGAGACAGTTCTCCAGCCAGGGACTa                                                                                                                         <  2:944370/140‑1 (MQ=255)                     tGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCTGCAGGCCAGCGAGACAGTTCTCCAGCCAGGGACTATTGAGt                                                                                                                   >  1:1010064/1‑140 (MQ=255)                                aTGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCTGCAGGCCAGCGAGACAGTTCTCCAGCCAGGGACTATTGAGTACGAACGCCAt                                                                                                        <  1:1109553/140‑1 (MQ=255)                                aTGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCTGCAGGCCAGCGAGACAGTTCTCCAGCCAGGGACTATTGAGTACGAACGCCAt                                                                                                        <  1:358762/140‑1 (MQ=255)                                aTGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCTGCAGGCCAACGAGACAGTTCTCCAGCCAGGGACTATTGAGTACGAACGCCAt                                                                                                        <  1:1091317/140‑1 (MQ=255)                                        gccgccGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCTGCAGGCCAGCGAGACAGTTCTCCAGCCAGGGACTATTGAGTACGAACGCCATCGACTTAc                                                                                                <  1:882145/140‑1 (MQ=255)                                         ccgccgTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCTGCAGGCCAGCGAGACAGTTCTCCAGCCAGGGACTATTGAGTACGAACGCCATCGACTTAcg                                                                                               >  2:1233417/1‑140 (MQ=255)                                         ccgccgTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCTGCAGGCCAGCGAGACAGTTCTCCAGCCAGGGACTATTGAGTACGAACGCCATCGACTTAcg                                                                                               >  1:39046/1‑140 (MQ=255)                                               tCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCTGCAGGCCAGCGAGACAGTTCTCCAGCCAAGGACTATTGAGTACGAGCGCCATCGACTTACGCGTgcg                                                                                         <  1:491131/140‑1 (MQ=255)                                                   aaaaTGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCTGCAGGCCAGCGAGACAGTTCTCCAGCCAGGGACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAgg                                                                                     >  1:1267737/1‑140 (MQ=37)                                                    aaaTGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCTGCAGGCCAGCGAGACAGTTCTCCAGCCAGGGACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGc                                                                                    <  1:477823/140‑1 (MQ=37)                                                         gTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCTGCAGGCCAGCGAGACAGTTCTCCAGCCAGGGACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCa                                                                                       >  1:307008/1‑132 (MQ=21)                                                         gTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCTGCAGGCCAGCGAGACAGTTCTCCAGCCAGGGACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCa                                                                                       <  2:307008/132‑1 (MQ=21)                                                          tATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCTGCAGGCCAGCGAGACAGTTCTCCAGCCAGGGACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGATGc                                                                              >  1:106558/1‑140 (MQ=37)                                                          tATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCTGCAGGCCAGCGAGACAGTTCTCCAGCCAGGGACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGATGc                                                                              >  2:736299/1‑140 (MQ=37)                                                               cGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCTGCAGGCCAGCGAGACAGTTCTCCAGCCAGGGACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGc                                                                                    <  1:78472/129‑1 (MQ=25)                                                               cGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCTGCAGGCCAGCGAGACAGTTCTCCAGCCAGGGACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGc                                                                                    >  2:78472/1‑129 (MQ=25)                                                               cGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCTGCAGGCCAGCGAGACAGTTCTCCAGCCAGGGACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGATGCACAgg                                                                         >  1:627684/1‑140 (MQ=37)                                                                 aaaGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCTGCAGGCCAGCGAGACAGTTCTCCAGCCAGGGACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGATGCACAGGAg                                                                       >  1:1337137/1‑140 (MQ=37)                                                                   aGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCTGCAGGCCAGCGAGACAGTTCTCCAGCCAGGGACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGc                                                                                    <  1:1152591/125‑1 (MQ=25)                                                                   aGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCTGCAGGCCAGCGAGACAGTTCTCCAGCCAGGGACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGc                                                                                    >  2:1152591/1‑125 (MQ=25)                                                                   aGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCTGCAGGCCAGCGAGACAGTTCTCCAGCCAGGGACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGATGCACAGGAGCt                                                                     >  1:698174/1‑140 (MQ=37)                                                                       aTGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCTGCAGGCCAGCGAGACAGTTCTCCAGCCAGGGACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGATGCACAGGAGCTGaaa                                                                 <  2:379179/140‑1 (MQ=37)                                                                        tGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCTGCAGGCCAGCGCGACAGTTCTCCAGCCAGGGACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGATGCACAGGAGCTGaaaa                                                                >  2:1001440/1‑140 (MQ=255)                                                                        tGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCTGCAGGCCAGCGAGACAGTTCTCCAGCCAGGGACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGATGCACAGGAGCTGaaaa                                                                >  1:434196/1‑140 (MQ=37)                                                                        tGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCTGCAGGCCAGCGAGACAGTTCTCCAGCCAGGGACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGATGCACAGGAGCTGaaaa                                                                <  1:23026/140‑1 (MQ=38)                                                                                  gagaACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCTGCAGGCCAGCGAGACAGTTCTCCAGCCAGGGACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCTGGCCGATGCACAGGAGCTGAAAAATGCCAgaga                                                      <  2:1010064/140‑1 (MQ=255)                                                                                    gaACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCTGCAGGCCAGCGAGACAGTTCTCCAGCCAGGGACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGATGCACAGGAGCTGAAAAATGCCAGAGACt                                                    >  1:1326403/1‑140 (MQ=38)                                                                                          aaaGCTGCGCCGGGAGGTTGAAGAACTGCTGCAGGCCAGCGAGACAGTTCTCCAGCCAGGGACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGATGCACAGGAGCTGAAAAATGCCAGAGACTCCGCTg                                              <  2:698174/140‑1 (MQ=38)                                                                                            aGCTGCGCCGGGAGGTTGAAGAACTGCTGCAGGCCAGCGAGACAGTTCTCCAGCCAGGGACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGATGCACAGGAGCTGAAAAATGCCAGAGACTCCGCTGaa                                            <  1:1233417/140‑1 (MQ=38)                                                                                                  gcCGGGAGGTTGAAGAACTGCTGCAGGCCAGCGAGACAGTTCTCCAGCCAGGGACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGATGCACAGGAGCTGAAAAATGCCAgaga                                                      >  1:863800/1‑124 (MQ=255)                                                                                                  gcCGGGAGGTTGAAGAACTGCTGCAGGCCAGCGAGACAGTTCTCCAGCCAGGGACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGATGCACAGGAGCTGAAAAATGCCAgaga                                                      <  2:863800/124‑1 (MQ=255)                                                                                                         ggTTGAAGAACTGCTGCAGGCCAGCGAGACAGTTCTCCAGCCAGGGACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGATGCACAGGAGCTGAAAAATGCCAGAGACTCCGCTGAAGTGGTGGAAACCg                               >  1:1306615/1‑140 (MQ=37)                                                                                                           ttGAAGAACTGCTGCAGGCCAGCGAGACAGTTCTCCAGCCAGGGACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGATGCACAGGAGCTGAAAAATGCCAGAGACTCCGCTGAAGTGGTGGAAACCGCa                             <  2:1337137/140‑1 (MQ=21)                                                                                                             gaagaaCTGCTGCAGGCCAGCGAGACAGTTCTCCAGCCAGGGACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGATGCACAGGAGCTGAAAAATGCCAGAGACTCCGCTGAAGTGGTGGAAACCGCAtt                           <  1:689959/140‑1 (MQ=21)                                                                                                             gaagaaCTGCTGCAGGCCAGCGAGACAGTTCTCCAGCCAGGGACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGATGCACAGGAGCTGAAAAATGCCAGAGACTCCGCTGAAGTGGTGGAAACCGCAtt                           <  1:1001440/140‑1 (MQ=21)                                                                                                                  aCTGCTGCAGGCCAGCGAGACAGTTCTCCAGCCAGGGACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGATGCACAGGAGCTGAAAAATGCCAGAGACTCCGCTGAAGTGGTGGAAACCGCATTCTGTa                      <  1:1114762/140‑1 (MQ=21)                                                                                                                  aCTGCTGCAGGCCAGCGAGACAGTTCTCCAGCCAGGGACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGATGCACAGGAGCTGAAAAATGCCAGAGACTCCGCTGAAGTGGTGGAAACCGCATTCTGTa                      >  1:1326588/1‑140 (MQ=21)                                                                                                                   ctgctgCAGGCCAGCGAGACAGTTCTCCAGCCAGGGACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGATGCACAGGAGCTGAAAAATGCCAGAGACTCCGCTGAAGTGGTGGAAACCGCATTCTGTAc                     >  1:585774/1‑140 (MQ=25)                                                                                                                      ctgcAGGCCAGCGAGACAGTTCTCCAGCCAGGGACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGATGCACAGGAGCTGAAAAATGCCAGAGACTCCGCTGAAGTGGTGGAAACCGCATTCTGTACttt                  >  1:768520/1‑140 (MQ=18)                                                                                                                          aGGCCAGCGAGACAGTTCTCCAGCCAGGGACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGATGCACAGGAGCTGAAAAATGCCAGAGACTCCGCTGAAGTGGTGGAAACCGCATTCTGTACTTTCGTg              <  1:736299/140‑1 (MQ=17)                                                                                                                           ggCCAGCGAGACAGTTCTCCAGCCAGGGACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGATGCACAGGAGCTGAAAAATGCCAGAGACTCCGCTGAAGTGGTGGAAACCGCATTCTGTACTTTCGTGc             <  1:720309/140‑1 (MQ=17)                                                                                                                                     aCAGTTCTCCAGCCAGGGACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGATGCACAGGAGCTGAAAAATGCCAGAGACTCCGCTGAAGTGGTGGAAACCGCATTCTGTACTTTCGTGCTg           >  2:416915/1‑132 (MQ=17)                                                                                                                                     aCAGTTCTCCAGCCAGGGACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGATGCACAGGAGCTGAAAAATGCCAGAGACTCCGCTGAAGTGGTGGAAACCGCATTCTGTACTTTCGTGCTg           <  1:416915/132‑1 (MQ=17)                                                                                                                                      cAGTTCTCCAGCCAGGGACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGATGCACAGGAGCTGAAAAATGCCAGAGACTCCGCTGAAGTGGTGGAAACCGCATTCTGTACTTTCGTGCTGTCGCGGAtt  >  1:498597/1‑139 (MQ=17)                                                                                                                                      cAGTTCTCCAGCCAGGGACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGATGCACAGGAGCTGAAAAATGCCAGAGACTCCGCTGAAGTGGTGGAAACCGCATTCTGTACTTTCGTGCTGTCGCGGATc  <  2:1326403/140‑1 (MQ=17)                                                                                                                                         |                                                                                                                                         CGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCTGCAGGCCAGCGAGACAGATCTCCAGCCAGGGACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGATGCACAGGAGCTGAAAAATGCCAGAGACTCCGCTGAAGTGGTGGAAACCGCATTCTGTACTTTCGTGCTGTCGCGGATC  >  NC_000913/580924‑581196

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑