Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	715,435	C→T	V8V (GTC→GTT)	ybfP →	lipoprotein YbfP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	715,435	0	C	T	96.7%	192.0 / ‑4.0	60	V8V (GTC→GTT)	ybfP	lipoprotein YbfP
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (34/24);  total (35/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTGCAATATATTGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTAGCTGGCGACTGGTTACTGACCCCTAAAGATAAAACCAGAGGATTAACGGGTAGCATTGCGGTAAAT  >  NC_000913/715299‑715570                                                                                                                                         |                                                                                                                                        ggTGCAATATATTGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGgttgtc                                                                                                                                      >  1:281523/1‑140 (MQ=255)  gTGCAATATATTGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTTGTCa                                                                                                                                     >  1:1271217/1‑140 (MQ=255)   tGCAATATATTGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTTGTCAt                                                                                                                                    >  1:21262/1‑140 (MQ=255)   tGCAATATATTGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTTGTCAt                                                                                                                                    <  2:21262/140‑1 (MQ=255)    gCAATATATTGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTTGTCATa                                                                                                                                   <  2:467606/140‑1 (MQ=255)       atatatTGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTTGTCATAgtg                                                                                                                                <  2:543634/140‑1 (MQ=255)            ttGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTTGTCATAGTGTCACt                                                                                                                           >  1:145622/1‑140 (MQ=255)             tGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTTGTCATAGTGTCACTa                                                                                                                          >  2:1135348/1‑140 (MQ=255)             tGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTTGTCATAGTGTCACTa                                                                                                                          >  1:698092/1‑140 (MQ=255)                  ttAAGCGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTTGTCATAGTGTCACTAATTAc                                                                                                                     >  2:661711/1‑140 (MQ=255)                    aaGCGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTTGTCATAGTGTCACTAATTACCg                                                                                                                   >  2:1394181/1‑140 (MQ=255)                      gCGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATAGTGTCACTAATTACCGcc                                                                                                                 >  2:286756/1‑140 (MQ=255)                           tttGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTTGTCATAGTGTCACTAATTACCGCCACAtt                                                                                                            >  1:1314238/1‑140 (MQ=255)                                    cGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTTGTCATAGTGTCACTAATTACCGCCACATTACTGCTGAc                                                                                                   >  1:1051906/1‑140 (MQ=255)                                    cGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTTGTCATAGTGTCACTAATTACCGCCACATTACTGCTGAc                                                                                                   >  2:210542/1‑140 (MQ=255)                                    cGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTTGTCATAGTGTCACTAATTACCGCCACATTACTGCTGAc                                                                                                   >  1:1273732/1‑140 (MQ=255)                                      gATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTTGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTg                                                                                                 >  2:819248/1‑140 (MQ=255)                                      gATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTTGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTg                                                                                                 >  1:482858/1‑140 (MQ=255)                                       aTAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTTGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGc                                                                                                >  1:773971/1‑140 (MQ=255)                                        tAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTTGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCa                                                                                               <  1:661711/140‑1 (MQ=255)                                             ggtccACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAACCCCAATGAAGACCAACAGGAGTCTGGTTGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATgcgc                                                                                          <  2:935291/136‑1 (MQ=255)                                             cGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTTGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATgcgc                                                                                          >  2:862645/1‑140 (MQ=255)                                                tCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTTGTCATAGTGTCACTAATTACCGCCACATTActg                                                                                                        >  1:47225/1‑123 (MQ=255)                                                tCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTTGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCa                                                                                       >  1:661479/1‑140 (MQ=255)                                                tCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTTGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCa                                                                                       >  2:1191513/1‑140 (MQ=255)                                                tCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTTGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCa                                                                                       >  1:696099/1‑140 (MQ=255)                                                tCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAACCCCAATGAAGACCAACAGGAGTCTGGTTGTCATAGTGTCACTAATTACCGCCACATTActg                                                                                                        <  2:47225/123‑1 (MQ=255)                                                 cACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTTGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGccc                                                                                        <  1:1184682/138‑1 (MQ=255)                                                 cACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTTGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGccc                                                                                        >  2:1184682/1‑138 (MQ=255)                                                 cACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTTGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCaa                                                                                      >  2:50487/1‑140 (MQ=255)                                                 cACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTTGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCaa                                                                                      >  1:824075/1‑140 (MQ=255)                                                          tCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTTGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATgcgc                                                                                          <  2:671701/127‑1 (MQ=255)                                                          tCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTTGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATgcgc                                                                                          >  1:671701/1‑127 (MQ=255)                                                          tCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTTGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACaa                                                                             <  2:773971/140‑1 (MQ=255)                                                           ccGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTTGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAat                                                                            <  1:1394181/140‑1 (MQ=255)                                                           ccGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAACCCCAATGAAGACCAACAGGAGTCTGGTTGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAat                                                                            <  1:199501/140‑1 (MQ=255)                                                                   aacaacGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTTGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTa                                                                    >  1:38633/1‑140 (MQ=255)                                                                       acGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTTGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTAGCTg                                                                >  2:1279512/1‑140 (MQ=255)                                                                        cgaccccTTTTTCAACAACCTGATGAGCTAAAGGAAACCCAAAGAAAGACAAAGGGGGGTCTGTTTTTCATGGTTTCACTATTTCCCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTAGCTgg                                                               <  2:696099/134‑1 (MQ=255)                                                                        cGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTTGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTAGCTgg                                                               >  1:688988/1‑140 (MQ=255)                                                                           acacTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTTGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTAGCTGGCGa                                                            <  2:1051906/140‑1 (MQ=255)                                                                           acacTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTTGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTAGCTGGCGa                                                            <  2:145622/140‑1 (MQ=255)                                                                                    caacaaACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTTGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTAGCTGGCGACTGGTTACt                                                   <  1:1135348/140‑1 (MQ=255)                                                                                    caacaaACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTTGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTAGCTGGCGACTGGTTACt                                                   <  1:1191513/140‑1 (MQ=255)                                                                                       caaACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTTGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTAGCTGGCGACTGGTTACTGAc                                                >  2:1256856/1‑140 (MQ=255)                                                                                           cTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTTGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTAGCTGGCGACTGGTTACTGACCCCt                                            <  1:1256856/140‑1 (MQ=255)                                                                                            tGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTTGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTAGCTGGCGACTGGTTACTGACCCCTa                                           <  1:819248/140‑1 (MQ=255)                                                                                             gAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTTGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTAGCTGGCGACTGGTTACTGACCCCTaa                                          >  1:209844/1‑140 (MQ=255)                                                                                                    tCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTTGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTAGCTGGCGACTGGTTACTGACCCCTAAAGATaaa                                   <  1:862645/140‑1 (MQ=255)                                                                                                        ggAAATCCCAATGAAGACCAACAGGAGTCTGGTTGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTAGCTGGCGACTGGTTACTGACCCCTa                                           <  1:1189868/128‑1 (MQ=255)                                                                                                        ggAAATCCCAATGAAGACCAACAGGAGTCTGGTTGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTAGCTGGCGACTGGTTACTGACCCCTa                                           >  2:1189868/1‑128 (MQ=255)                                                                                                        ggAAATCCCAATGAAGACCAACAGGAGTCTGGTTGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTAGCTGGCGACTGGTTACTGACCCCTAAAGATAAAACCa                               <  2:482858/140‑1 (MQ=255)                                                                                                        ggAAATCCCAATGAAGACAAACAGGAGTCTGGTTGTCATAGTGTCACTAATTACAGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTAGCTGGCGACTGGTTACTGACCCTTAAAGATAAAACCa                               >  2:858310/1‑140 (MQ=255)                                                                                                             tCCCAATGAAGACCAACAGGAGTCTGGTTGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTAGCTGGCGACTGGTTACTGACCCCTAAAGATAAAACCAGAGGa                          >  1:1102008/1‑140 (MQ=255)                                                                                                                cAATGAAGACCAACAGGAGTCTGGTTGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTAGCTGGCGACTGGTTACTGACCCCTAAAGATAAAACCAGAGGAtca                       <  1:736950/140‑3 (MQ=255)                                                                                                                cAATGAAGACCAACAGGAGTCTGGTCGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTAGCTGGCGACTGGTTACTGACCCCTAAAGATAAAACCAGAGGATTa                       <  1:286756/140‑1 (MQ=255)                                                                                                                  aTGAAGACCAACAGGAGTCTGGTTGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTAGCTGGCGACTGGTTACTGACCCCTAAAGATAAAACCAGAGGATTGAc                     >  2:1161593/1‑140 (MQ=255)                                                                                                                          cAACAGGAGTCTGGTTGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTAGCTGGCGACTGGTTACTGACCCCTAAAGATAAAACCAGAGGATTAACGGGTAGCa             <  2:661479/140‑1 (MQ=255)                                                                                                                            aCAGGAGTCTGGTTGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTAGCTGGCGACTGGTTACTGACCCCTAAAGATAAAACCAGAGGATTAACGGGTAGCAtt           <  2:633543/140‑1 (MQ=255)                                                                                                                                 aGTCTGGTTGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTAGCTGGCGACTGGTTACTGACCCCTAAAGATAAAACCAGAGGATTAACGGGTAGCATTGCGGt      <  2:209402/140‑1 (MQ=255)                                                                                                                                  gTCTGGTTGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTAGCTGGCGACTGGTTACTGACCCCTAAAGATAAAACCAGAGGATTAACGGGTAGCATTGCg        <  2:1168111/137‑1 (MQ=255)                                                                                                                                  gTCTGGTTGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTAGCTGGCGACTGGTTACTGACCCCTAAAGATAAAACCAGAGGATTAACGGGTAGCATTGCg        >  1:1168111/1‑137 (MQ=255)                                                                                                                                     tGGTTGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTAGCTGGCGACTGGTTACTGACCCCTAAAGATAAAACCAGAGGATTAACGGGTAGCATTGCGGTAAat  >  1:1309348/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        GGTGCAATATATTGAATTTAAGCGATTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTAGCTGGCGACTGGTTACTGACCCCTAAAGATAAAACCAGAGGATTAACGGGTAGCATTGCGGTAAAT  >  NC_000913/715299‑715570

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑