Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	860,724	A→G	L628P (CTG→CCG)	ybiW ←	putative pyruvate formate lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	860,724	0	A	G	100.0%	267.5 / NA	74	L628P (CTG→CCG)	ybiW	putative pyruvate formate lyase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (38/36);  total (38/36)

CTGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCATCGTCGTTGCCGTACTTCGGCGCACCGTTAATCAGCCGCTGACGCAGCTGCTCGTGAGTCAGGCCGTCGAAGTCATCTGCCAGTGCGGCAGCAAGCTGTTGCTGACCAATCGCACCT  >  NC_000913/860588‑860858                                                                                                                                         |                                                                                                                                       cTGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAgcggcg                                                                                                                                     >  1:381657/1‑140 (MQ=255) cTGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAgcggcg                                                                                                                                     >  1:27028/1‑140 (MQ=255) cTGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAgcggcg                                                                                                                                     >  2:232089/1‑140 (MQ=255)   gcgcgcCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCGGCGTa                                                                                                                                   <  2:1226809/140‑1 (MQ=255)    cgcgcCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCGGCGTAt                                                                                                                                  >  2:142380/1‑140 (MQ=255)       gcCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCGGCGTATCGa                                                                                                                               <  2:569013/140‑1 (MQ=255)       gcCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCGGCGTATCGa                                                                                                                               <  2:859723/140‑1 (MQ=255)       gcCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCGGCGTATCGa                                                                                                                               <  2:829726/140‑1 (MQ=255)         cAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCGGCGTATCGACa                                                                                                                             >  2:529068/1‑140 (MQ=255)         cAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCGGCGTATCGACa                                                                                                                             >  1:676343/1‑140 (MQ=255)                tACGTTAGCGGAGATTGATGACGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCGGCGTATCGACAGTatcat                                                                                                                      >  2:201926/1‑140 (MQ=255)                   gTTAGCGGAGATTGATGACGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCAAGCGGCGTATCGCCAGTATCAtcgt                                                                                                                   >  2:1212196/1‑140 (MQ=255)                     tAGCGGAGATTGATGACGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCGGCGTATCGACAGTATCAtcgtcg                                                                                                                 >  2:914099/1‑140 (MQ=255)                     tAGCGGAGATTGATGACGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCGGCGTATCGACAGTATCAtcgtcg                                                                                                                 >  2:1005935/1‑140 (MQ=255)                     tAGCGGAGATTGATGACGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCGGCGTATCGACAGTATCAtcgtcg                                                                                                                 <  2:216854/140‑1 (MQ=255)                     tAGCGGAGATTGATGACGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCGGCGTATCGACAGTATCAtcgtcg                                                                                                                 <  1:751776/140‑1 (MQ=255)                        cGGAGATTGATGACGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCGGCGTATCGACAGTATCATCGTCGTTg                                                                                                              <  1:914099/140‑1 (MQ=255)                        cGGAGATTGATGACGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCGGCGTATCGACAGTATCATCGTCGTTg                                                                                                              <  1:1005935/140‑1 (MQ=255)                                   gaCGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCGGCGTATCGACAGTATCATCGTCGTTGCCGTACTTCgg                                                                                                   <  1:746208/140‑1 (MQ=255)                                     cGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCGGCGTATCGACAGTATCATCGTCGTTGCCGTACTTCGgcg                                                                                                 <  2:676343/140‑1 (MQ=255)                                     cGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCCTCGATATAGGTCTGATAAGCGCGAGCCAGCGGCGTATCGACAGTATCATCGTCGTTGCCGTACTTCGgcg                                                                                                 <  1:1212196/140‑1 (MQ=255)                                       tACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCGGCGTATCGACAGTATCATCGTCGTTg                                                                                                              <  1:383072/125‑1 (MQ=255)                                       tACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCGGCGTATCGACAGTATCATCGTCGTTGCCGTACTTCGGCGCa                                                                                               <  2:371139/140‑1 (MQ=255)                                       tACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCGGCGTATCGACAGTATCATCGTCGTTGCCGTACTTCGGCGCa                                                                                               <  2:296976/140‑1 (MQ=255)                                       tACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCGGCGTATCGACAGTATCATCGTCGTTGCCGTACTTCGGCGCa                                                                                               >  2:721348/1‑140 (MQ=255)                                       tACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCGGCGTATCGACAGTATCATCGTCGTTGCCGTACTTCGGCGCa                                                                                               >  2:1318788/1‑140 (MQ=255)                                       tACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCGGCGTATCGACAGTATCATCGTCGTTGCCGTACTTCGGCGCa                                                                                               >  1:1116354/1‑140 (MQ=255)                                       tACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGCTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCGGCGTATCGACAGTATCATCGTCGTTg                                                                                                              >  2:383072/1‑125 (MQ=255)                                        aCCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCGGCGTATCGACAGTATCATCGTCGTTGCCGTACTTCGGCGCAc                                                                                              <  2:457593/140‑1 (MQ=255)                                         gccacgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCGGCGTATCGACAGTATCATCGTCGTTGCCGTACTTCGgcg                                                                                                 >  2:831439/5‑136 (MQ=255)                                         cccGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCGGCGTATCGACAGTATCATCGTCGTTGCCGTACTTCGgcg                                                                                                 <  1:831439/136‑1 (MQ=255)                                          ccGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCGGCGTATCGACAGTATCATCGTCGTTGCCGTACTTCGGCGCACCg                                                                                            <  1:529068/140‑1 (MQ=255)                                                aaTAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCGGCGTATCGACAGTATCATCGTCGTTGCCGTACTTCGGCGCACCGTTAATc                                                                                      >  2:186935/1‑140 (MQ=255)                                                 aTAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCGGCGTATCGACAGTATCGTCGTCGTTGCCGTACTTCGGCGCACCGTTAATCa                                                                                     <  1:695512/140‑1 (MQ=255)                                                   aGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCGGCGTATCGACAGTATCATCGTCGTTGCCGTACTTCGGCGCACCGTTAATCAGc                                                                                   >  2:358912/1‑140 (MQ=255)                                                    gTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCGGCGTATCGACAGTATCATCGTCGTTGCCGTACTTCGGCGCACCGTTAATCAGcc                                                                                  >  2:1292439/1‑140 (MQ=255)                                                    gTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCGGCGTATCGACAGTATCATCGTCGTTGCCGTACTTCGGCGCACCGTTAATCAGcc                                                                                  >  2:347740/1‑140 (MQ=255)                                                    gTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCGGCGTATCGACAGTATCATCGTCGTTGCCGTACTTCGGCGCACCGTTAATCAGcc                                                                                  >  2:1086410/1‑140 (MQ=255)                                                     ttGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCGGCGTATCGACAGTATCATCGTCGTTGCCGTACTTCGGCGCACCGTTAATCAGCCg                                                                                 <  1:347740/140‑1 (MQ=255)                                                       gccgccAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCGGCGTATCGACAGTATCATCGTCGTTGCCGTACTTCGGCGCACCGTTAATCAGCCGCt                                                                               >  1:842657/1‑140 (MQ=255)                                                       gccgccAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCGGCGTATCGACAGTATCATCGTCGTTGCCGTACTTCGGCGCACCGTTAATCAGCCGCt                                                                               >  1:552686/1‑140 (MQ=255)                                                         cgccAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCGGCGTATCGACAGTATCATCGTCGTTGCCGTACTTCGGCGCACCGTTAATCAGCCGCTGa                                                                             <  2:27028/140‑1 (MQ=255)                                                            cAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCGGCGTATCGACAGTATCATCGTCGTTGCCGTACTTCGGCGCACCGTTAATCAGCCGCTGACGc                                                                          <  1:201926/140‑1 (MQ=255)                                                              aCCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCGGCGTATCGACAGTATCATCGTCGTTGCCGTACTTCGGCGCACCGTTAATCAGCCGCTGACGCAg                                                                        >  2:864768/1‑140 (MQ=255)                                                                  gACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCGGCGTATCGACAGTATCATCGTCGTTGCCGTACTTCGGCGCACCGTTAATCAGCCGCTGAc                                                                            >  1:1245129/1‑132 (MQ=255)                                                                  gACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCGGCGTATCGACAGTATCATCGTCGTTGCCGTACTTCGGCGCACCGTTAATCAGCCGCTGAc                                                                            <  2:1245129/132‑1 (MQ=255)                                                                   aCCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCGGCGTATCGACAGTATCATCGTCGTTGCCGTACTTCGGCGCACCGTTAATCAGCCGCTGACGCAgctgct                                                                   >  1:1169873/1‑140 (MQ=255)                                                                    ccACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCGGCGTATCGACAGTATCATCGTCGTTGCCGTACTTCGGCGCACCGTTAATCAGCCGCTGACGCAGCTGCTc                                                                  <  1:126657/140‑1 (MQ=255)                                                                    ccACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCGGCGTATCGACAGTATCATCGTCGTTGCCGTACTTCGGCGCACCGTTAATCAGCCGCTGACGCAGCTGCTc                                                                  <  1:721348/140‑1 (MQ=255)                                                                     cACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCGGCGTATCGACAGTATCATCGTCGTTGCCGTACTTCGGCGCACCGTTAATCAGCCGCTGACGCAGCTGCTCg                                                                 <  1:37487/140‑1 (MQ=255)                                                                      aCGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCGGCGTATCGACAGTATCATCGTCGTTGCCGTACTTCGGCGCACCGTTAATCAGCCGCTGACGCAGCTGCTCGt                                                                <  2:381657/140‑1 (MQ=255)                                                                        gACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCGGCGTATCGACAGTATCATCGTCGTTGCCGTACTTCGGCGCACCGTTAATCAGCCGCTGACGCAGCTGCTCGTGa                                                              >  2:7260/1‑140 (MQ=255)                                                                              aGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCGGCGTATCGACAGTATCATCGTCGTTGCCGTACTTCGGCGCACCGTTAATCAGCCGCTGACGCAGCTGCTCGTGAGTCAgg                                                        <  2:1218189/140‑1 (MQ=255)                                                                                  cgGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCGGCGTATCGACAGTATCATCTCGGATGCCGTACTTTGCTTGACCAATAATCAGCCGCTGACGCAGCTGCTCGTGAGTCAGGCCGt                                                    >  1:763589/1‑140 (MQ=255)                                                                                  cgGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCGGCGTATCGACAGTATCATCGTCGTTGCCGTACTTCGGCGCACCGTTAATCAGCCGCTGACGCAGCTGCTCGTGAGTCAGGCCGt                                                    >  2:494853/1‑140 (MQ=255)                                                                                     aTTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCGGCGTATCGACAGTATCATCGTCGTTGCCGTACTTCGGCGCACCGTTAATCAGCCGCTGACGCAGCTGCTCGTGAGTCAGGCCGTCGa                                                 <  2:552686/140‑1 (MQ=255)                                                                                     aTTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCGGCGTATCGACAGTATCATCGTCGTTGCCGTACTTCGGCGCACCGTTAATCAGCCGCTGACGCAGCTGCTCGTGAGTCAGGCCGTCGa                                                 <  1:864768/140‑1 (MQ=255)                                                                                               tGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCGGCGTATCGACAGTATCATCGTCGTTGCCGTACTTCGGCGCACCGTTAATCAGCCGCTGACGCAGCTGCTCGTGAGTCAGGCCGTCGAAGTCATCTGc                                       >  1:1190309/1‑140 (MQ=255)                                                                                               tGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCGGCGTATCGACAGTATCATCGTCGTTGCCGTACTTCGGCGCACCGTTAATCAGCCGCTGACGCAGCTGCTCGTGAGTCAGGCCGTCGAAGTCATCTGc                                       >  2:1350293/1‑140 (MQ=255)                                                                                               tGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCGGCGTATCGACAGTATCATCGTCGTTGCCGTACTTCGGCGCACCGTTAATCAGCCGCTGACGCAGCTGCTCGTGAGTCAGGCCGTCGAAGTCATCTGc                                       <  2:265232/140‑1 (MQ=255)                                                                                               tGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCGGCGTATCGACAGTATCATCGTCGTTGCCGTACTTCGGCGCACCGTTAATCAGCCGCTGACGCAGCTGCTCGTGAGTCAGGCCGTCGAAGTCATCTGc                                       >  1:497290/1‑140 (MQ=255)                                                                                                 tttCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCGGCGTATCGACAGTATCATCGTCGTTGCCGTACTTCGGCGCACCGTTAATCAGCCGCTGACGCAGCTGCTCGTGAGTCAGGCCGTCGAAGTCATCTGCCa                                     >  2:1339619/1‑140 (MQ=255)                                                                                                 tttCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCGGCGTATCGACAGTATCATCGTCGTTGCCGTACTTCGGCGCACCGTTAATCAGCCGCTGACGCAGCTGCTCGTGAGTCAGGCCGTCGAAGTCATCTGCCa                                     >  1:1234005/1‑140 (MQ=255)                                                                                                      gTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCGGCGTATCGACAGTATCATCGTCGTTGCCGTACTTCGGCGCACCGTTAATCAGCCGCTGACGCAGCTGCTCGTGAGTCAGGCCGTCGAAGTCATCTGCCAGTGCg                                >  1:922656/1‑140 (MQ=255)                                                                                                         cgtcgATATAGGTCTGATAAGCGCGAGCCAGCGGCGTATCGACAGTATCATCGTCGTTGCCGTACTTCGGCGCACCGTTAATCAGCCGCTGACGCAGCTGCTCGTGAGTCAGGCCGTCGAAGTCATCTGCCAGTGCGgca                             >  2:526053/1‑140 (MQ=255)                                                                                                          gtcgATATAGGTCTGATAAGCGCGAGCCAGCGGCGTATCGACAGTATCATCGTCGTTGCCGTACTTCGGCGCACCGTTAATCAGCCGCTGACGCAGCTGCTCGTGAGTCAGGCCGTCGAAGTCATCTGCCAGTGCGgcag                            <  1:1350293/140‑1 (MQ=255)                                                                                                           tcgATATAGGTCTGATAAGCGCGAGCCAGCGGCGTATCGACAGTATCATCGTCGTTGCCGTACTTCGGCGCACCGTTAATCAGCCGCTGACGCAGCTGCTCGTGAGTCAGGCCGTCGAAGTCATCTGCCAGTGCGgcagc                           <  1:494853/140‑1 (MQ=255)                                                                                                           tcgATATAGGTCTGATAAGCGCGAGCCAGCGGCGTATCGACAGTATCATCGTCGTTGCCGTACTTCGGCGCACCGTTAATCAGCCGCTGACGCAGCTGCTCGTGAGTCAGGCCGTCGAAGTCATCTGCCAGTGCGgcagc                           >  1:938251/1‑140 (MQ=255)                                                                                                              atatAGGTCTGATAAGCGCGAGCCAGCGGCGTATCGACAGTATCATCGTCGTTGCCGTACTTCGGCGCGCCGTTAATCAGCCGCTGACGCAGCTGCTCGTGAGTCAGGCCGTCGAAGTCATCTGCCAGTGCGGCAGCAAg                        <  2:938251/140‑1 (MQ=255)                                                                                                                     tCTGATAAGCGCGAGCCAGCGGCGTATCGACAGTATCATCGTCGTTGCCGTACTTCGGCGCACCGTTAATCAGCCGCTGACGCAGCTGCTCGTGAGTCAGGCCGTCGAAGTCATCTGCCAGTGCGGCAGCAAGCTGTTGc                 >  2:199799/1‑140 (MQ=255)                                                                                                                        gATAAGCGCGAGCCAGCGGCGTATCGACAGTATCATCGTCGTTGCCGTACTTCGGCGCACCGTTAATCAGCCGCTGACGCAGCTGCTCGTGAGTCAGGCCGTCGAAGTCATCTGCCAGTGCGGCAGCAAGCTGTTGCTGa              >  1:894281/1‑140 (MQ=255)                                                                                                                        gATAAGCGCGAGCCAGCGGCGTATCGACAGTATCATCGTCGTTGCCGTACTTCGGCGCACCGTTAATCAGCCGCTGACGCAGCTGCTCGTGAGTCAGGCCGTCGAAGTCATCTGCCAGTGCGGCAGCAAGCTGTTGCTGa              >  1:1100025/1‑140 (MQ=255)                                                                                                                         aTAAGCGCGAGCCAGCGGCGTATCGACAGTATCATCGTCGTTGCCGTACTTCGGCGCACCGTTAATCAGCCGCTGACGCAGCTGCTCGTGAGTCAGGCCGTCGAAGTCATCTGCCAGTGCGGCAGCAAGCTGTTGCTGAc             >  1:784029/1‑140 (MQ=255)                                                                                                                         aTAAGCGCGAGCCAGCGGCGTATCGACAGTATCATCGTCGTTGCCGTACTTCGGCGCACCGTTAATCAGCCGCTGACGCAGCTGCTCGTGAGTCAGGCCGTCGAAGTCATCTGCCAGTGCGGCAGCAAGCTGTTGCTGAc             <  1:142380/140‑1 (MQ=255)                                                                                                                         aTAAGCGCGAGCCAGCGGCGTATCGACAGTATCATCGTCGTTGCCGTACTTCGGCGCACCGTTAATCAGCCGCTGACGCAGCTGCTCGTGAGTCAGGCCGTCGAAGTCATCTGCCAGTGCGGCAGCAAGCTGTTGCTGAc             <  1:1339619/140‑1 (MQ=255)                                                                                                                                   gCCAGCGGCGTATCGACAGTATCATCGTCGTTGCCGTACTTCGGCGCACCGTTAATCAGCCGCTGACGCAGCTGCTCGTGAGTCAGGCCGTCGAAGTCATCTGCCAGTGCGGCAGCAAGCTGTTGCTGACCAATCGCAcc   >  2:311248/1‑140 (MQ=255)                                                                                                                                   gCCAGCGGCGTATCGACAGTATCATCGTCGTTGCCGTACTTCGGCGCACCGTTAATCAGCCGCTGACGCAGCTGCTCGTGAGTCAGGCCGTCGAAGTCATCTGCCAGCGCGGCAGCAAGCTGTTGCTGACCAATCGCAcc   >  2:326424/1‑140 (MQ=255)                                                                                                                                    ccAGCGGCGTATCGACAGTATCATCGTCGTTGCCGTACTTCGGCGCACCGTTAATCAGCCGCTGACGCAGCTGCTCGTGAGTCAGGCCGTCGAAGTCATCTGCCAGTGCGGCAGCAAGCTGTTGCTGACCAATCGCACCt  <  1:1292439/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                       CTGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCATCGTCGTTGCCGTACTTCGGCGCACCGTTAATCAGCCGCTGACGCAGCTGCTCGTGAGTCAGGCCGTCGAAGTCATCTGCCAGTGCGGCAGCAAGCTGTTGCTGACCAATCGCACCT  >  NC_000913/860588‑860858

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑