Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	951,357	A→G	V733A (GTA→GCA)	pflB ←	pyruvate formate‑lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	951,357	0	A	G	100.0%	340.0 / NA	92	V733A (GTA→GCA)	pflB	pyruvate formate‑lyase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (57/35);  total (57/35)

CGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGA  >  NC_000913/951221‑951491                                                                                                                                         |                                                                                                                                       cGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACACAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATgcac                                                                                                                                     <  1:1195307/140‑1 (MQ=255)   tGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATGCACgg                                                                                                                                   >  1:1017135/1‑140 (MQ=255)      aGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATGCACGGATg                                                                                                                                >  2:1093008/1‑140 (MQ=255)         cTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATGCACGGATGATc                                                                                                                             <  2:686901/140‑1 (MQ=255)          tttATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATGCACGGATGGTCa                                                                                                                            >  1:308221/1‑140 (MQ=255)                  aCGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGa                                                                                                                    >  2:430452/1‑140 (MQ=255)                    gCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGata                                                                                                                  <  1:1065498/140‑1 (MQ=255)                      tttttACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGATAtt                                                                                                                >  1:616512/1‑140 (MQ=255)                        tttACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGATAtttt                                                                                                              >  1:1210132/1‑140 (MQ=255)                        tttACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGATAtttt                                                                                                              >  2:787309/1‑140 (MQ=255)                           aCTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGATATTTTTcc                                                                                                           <  1:786579/140‑1 (MQ=255)                           aCTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGATATTTTTcc                                                                                                           <  1:58806/140‑1 (MQ=255)                               tACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGATATTTTTCCGGGt                                                                                                       >  1:219379/1‑140 (MQ=255)                               tACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGATATTTTTCCGGGt                                                                                                       >  1:1160967/1‑140 (MQ=255)                                aCGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGATATTTTTCCGGGtt                                                                                                      >  1:1255286/1‑140 (MQ=255)                                aCGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGATATTTTTCCGGGtt                                                                                                      >  1:407290/1‑140 (MQ=255)                                         gTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATcgc                                                                                             >  2:1000347/1‑140 (MQ=255)                                         gTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATcgc                                                                                             >  2:795522/1‑140 (MQ=255)                                         gTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATcgc                                                                                             >  1:1341229/1‑140 (MQ=255)                                         gTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATcgc                                                                                             >  1:271500/1‑140 (MQ=255)                                                   aTTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATcgcg                                                                                            >  1:868126/1‑131 (MQ=255)                                                   aTTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATcgcg                                                                                            <  2:868126/131‑1 (MQ=255)                                                       cATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCAttt                                                                               <  1:1341535/140‑1 (MQ=255)                                                        aTAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTc                                                                              >  2:446040/1‑140 (MQ=255)                                                        aTAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTc                                                                              >  1:1367263/1‑140 (MQ=255)                                                        aTAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTc                                                                              >  2:751182/1‑140 (MQ=255)                                                         tAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCa                                                                             <  1:1000347/140‑1 (MQ=255)                                                           gATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACg                                                                           >  1:1351194/1‑140 (MQ=255)                                                            aTTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACgg                                                                          >  1:918739/1‑140 (MQ=255)                                                            aTTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACgg                                                                          >  1:952471/1‑140 (MQ=255)                                                            aTTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACgg                                                                          >  1:1185508/1‑140 (MQ=255)                                                             ttGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGt                                                                         >  1:344143/1‑140 (MQ=255)                                                             ttGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGt                                                                         >  2:129068/1‑140 (MQ=255)                                                              tGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGtt                                                                        >  1:597219/1‑140 (MQ=255)                                                                 gTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTtcat                                                                     <  2:308221/140‑1 (MQ=255)                                                                 gTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTtcat                                                                     >  1:1029868/1‑140 (MQ=255)                                                                  tGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTtca                                                                      >  1:1235299/1‑138 (MQ=255)                                                                  tGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTtca                                                                      <  2:1235299/138‑1 (MQ=255)                                                                      ggTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGt                                                                >  2:812635/1‑140 (MQ=255)                                                                      ggTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGt                                                                >  2:1066141/1‑140 (MQ=255)                                                                      ggTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGt                                                                >  1:742687/1‑140 (MQ=255)                                                                      ggTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGt                                                                <  2:639894/140‑1 (MQ=255)                                                                        tACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCAc                                                                            <  1:999299/126‑1 (MQ=255)                                                                        tACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCAc                                                                            >  2:999299/1‑126 (MQ=255)                                                                         aCGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTaa                                                             <  1:446040/140‑1 (MQ=255)                                                                           gAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACg                                                           >  1:805791/1‑140 (MQ=255)                                                                              taataaCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTaa                                                             <  2:975840/135‑1 (MQ=255)                                                                              taataaCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTaa                                                             >  2:969018/1‑135 (MQ=255)                                                                              taataaCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTaa                                                             <  1:969018/135‑1 (MQ=255)                                                                              taataaCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTaa                                                             >  1:975840/1‑135 (MQ=255)                                                                              taataaCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTc                                                        <  2:168428/140‑1 (MQ=255)                                                                              taataaCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTc                                                        >  1:128261/1‑140 (MQ=255)                                                                              taataaCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCACTTCACGGTTCATCACGTTAACGTTc                                                        >  1:116609/1‑140 (MQ=255)                                                                              taataaCGTCCTGCTGCTGTTCTTTAGTCAGCGAGGTGAAACGTACTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGATATTTTTCCGGGGTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGttt                                                        >  2:947830/1‑139 (MQ=255)                                                                                ataaCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAg                                                      <  2:318152/140‑1 (MQ=255)                                                                                 taaCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTc                                                        >  1:509923/1‑137 (MQ=255)                                                                                 taaCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTc                                                        <  2:509923/137‑1 (MQ=255)                                                                                 taaCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAgg                                                     >  1:727499/1‑140 (MQ=255)                                                                                 taaCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAgg                                                     >  1:232522/1‑140 (MQ=255)                                                                                      tCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTg                                                <  2:232522/140‑1 (MQ=255)                                                                                      tCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTg                                                <  2:727499/140‑1 (MQ=255)                                                                                       ccTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGa                                               <  2:1029868/140‑1 (MQ=255)                                                                                       ccTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGa                                               >  2:775030/1‑140 (MQ=255)                                                                                       ccTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGa                                               >  2:1375007/1‑140 (MQ=255)                                                                                        ctgctgctgTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGac                                              >  1:492406/1‑140 (MQ=255)                                                                                        ctgctgctgTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGac                                              >  1:1178895/1‑140 (MQ=255)                                                                                         tgctgctgTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGacc                                             <  2:840994/140‑1 (MQ=255)                                                                                         tgctgctgTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGacc                                             <  1:1008284/140‑1 (MQ=255)                                                                                          gctgctgTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGacca                                            >  1:799165/1‑140 (MQ=255)                                                                                          gctgctgTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGacca                                            <  1:787309/140‑1 (MQ=255)                                                                                          gctgctgTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGacca                                            >  2:323067/1‑140 (MQ=255)                                                                                           ctgctgTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTg                                                   <  2:1344583/132‑1 (MQ=255)                                                                                           ctgctgTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTg                                                   >  1:1344583/1‑132 (MQ=255)                                                                                           ctgctgTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGaccac                                           >  1:1011841/1‑140 (MQ=255)                                                                                            tgctgTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGaccacc                                          <  1:1375007/140‑1 (MQ=255)                                                                                                 ttCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCga                                     >  1:362258/1‑140 (MQ=255)                                                                                                     ttAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCgatgga                                 <  2:742687/140‑1 (MQ=255)                                                                                                      tAGTCAGAGAGTTGAAACGTACTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCgatggat                                >  2:997681/1‑140 (MQ=255)                                                                                                        gTCAGCGAGTTGAAACGTACTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGaccacc                                          <  1:353146/128‑1 (MQ=255)                                                                                                        gTCAGCGAGTTGAAACGTACTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGaccacc                                          >  2:353146/1‑128 (MQ=255)                                                                                                        gTCAGCGAGTTGAAACGTACTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGc                              >  1:1273679/1‑140 (MQ=255)                                                                                                          cAGCGAGTTGAAACGTACTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCtt                            <  1:751182/140‑1 (MQ=255)                                                                                                           aGCGAGTTGAAACGTACTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTc                           >  2:696465/1‑140 (MQ=255)                                                                                                            gCGAGTTGAAACGTACTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCg                          <  2:362258/140‑1 (MQ=255)                                                                                                            gCGAGTTGAAACGTACTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCg                          <  1:207642/140‑1 (MQ=255)                                                                                                              gAGTTGAAACGTACTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCgtg                        >  2:36321/1‑140 (MQ=255)                                                                                                              gAGTTGAAACGTACTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCgtg                        <  1:67329/140‑1 (MQ=255)                                                                                                                          aCTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGt                >  1:640921/1‑136 (MQ=255)                                                                                                                          aCTGCGTAGCCAGATGCACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGt                <  2:640921/136‑1 (MQ=255)                                                                                                                           cTGCGTAGCCAGATGCTCGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAAcca           <  2:1255286/140‑1 (MQ=255)                                                                                                                              cGTAGCCAGATGCACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAAccatcc        >  2:383911/1‑140 (MQ=255)                                                                                                                                tAGCCAGATGCACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAAccatccat      <  2:1178895/140‑1 (MQ=255)                                                                                                                                   ccAGATGCACGGATGGTCAGCTGCGGATATTTTTCCTGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAg   >  1:991849/1‑140 (MQ=255)                                                                                                                                    cAGATGCACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGa  <  1:430452/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                       CGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGA  >  NC_000913/951221‑951491

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑