Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,035,876	C→T	P36L (CCC→CTC)	hyaD →	putative hydrogenase 1 maturation protease HyaD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,035,876	0	C	T	100.0%	194.2 / NA	55	P36L (CCC→CTC)	hyaD	putative hydrogenase 1 maturation protease HyaD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/35);  total (20/35)

AATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACC  >  NC_000913/1035739‑1036003                                                                                                                                          |                                                                                                                                aaTTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCTCg                                                                                                                               <  2:1134203/140‑1 (MQ=255)  aTTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCTCGa                                                                                                                              <  1:851946/140‑1 (MQ=255)   tttGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCTCg                                                                                                                               >  2:388939/1‑138 (MQ=255)   tttGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCTCGAg                                                                                                                             <  2:131918/140‑1 (MQ=255)   tttGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCTCGAg                                                                                                                             <  1:873818/140‑1 (MQ=255)   tttGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCTCGAg                                                                                                                             <  2:217815/140‑1 (MQ=255)          aaaTAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCTCGAGTATGTgg                                                                                                                      <  2:1150227/140‑1 (MQ=255)             tAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCTCGAGTATGTGgaga                                                                                                                   <  1:679746/140‑1 (MQ=255)                  aaCAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCTCGAGTATGTGGAGATTGTc                                                                                                              <  2:264518/140‑1 (MQ=255)                    cAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCAGCGTGCGGGTGGCGGAACGGCTGTATGGTAATTGCCACTGGCTCGAGTATGTGGTGATTGTCGa                                                                                                            <  2:901537/140‑1 (MQ=255)                     aaGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCTCGAGTATGTGGAGATTGTCGAt                                                                                                           >  1:967986/1‑140 (MQ=255)                       ggAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCTCGAGTATGTGGAGATTGTCGATgg                                                                                                         <  1:762341/140‑1 (MQ=255)                         aGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCTCGAGTATGTGGAGATTGTCGATGGCg                                                                                                       >  1:1007696/1‑140 (MQ=255)                           cGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCTCGAGTATGTGGAGATTGTCGATGGCGGt                                                                                                     >  2:1364817/1‑140 (MQ=255)                             ttCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCTCGAGTATGTGGAGATTGTCGATGGCGGTAc                                                                                                   >  2:528862/1‑140 (MQ=255)                               cATGAGCGAGCAACGCGTGGGGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACAGCTGTATGGCCATTCCCCCTGGGTCGAGTATGTGGAGATTGTCGATGGCGGTCCTc                                                                                                 <  2:35704/140‑1 (MQ=255)                                aTGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCTCGAGTATGTGGAGATTGTCGATGGCGGTACTCa                                                                                                <  2:1245494/140‑1 (MQ=255)                                aTGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCTCGAGTATGTGGAGATTGTCGATGGCGGTACTCa                                                                                                <  2:474149/140‑1 (MQ=255)                                     cgagcAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCTCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGAc                                                                                           <  1:756763/140‑1 (MQ=255)                                       agcAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCTCGAGTATGTGGAGATTGTCGATgg                                                                                                         <  2:533164/124‑1 (MQ=255)                                       agcAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCTCGAGTATGTGGAGATTGTCGATgg                                                                                                         >  1:533164/1‑124 (MQ=255)                                       agcAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCTCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTg                                                                                         >  1:544381/1‑140 (MQ=255)                                        gcAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGTCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCTCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGa                                                                                        <  2:1027386/140‑1 (MQ=255)                                         cAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCTCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGaa                                                                                       <  2:1108070/140‑1 (MQ=255)                                              cgTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCTCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGc                                                                                  >  2:56022/1‑140 (MQ=255)                                                  gtggtCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCTCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTggg                                                                              <  1:1364817/140‑1 (MQ=255)                                                  gtggtCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCTCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTggg                                                                              >  2:667280/1‑140 (MQ=255)                                                  gtggtCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCTCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTggg                                                                              >  2:1150719/1‑140 (MQ=255)                                                          ggggCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCTCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCg                                                                      <  2:1007696/140‑1 (MQ=255)                                                              cTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCTCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAg                                                                  <  1:43010/140‑1 (MQ=255)                                                                gggCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCTCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAgcg                                                                <  2:1392368/140‑1 (MQ=255)                                                                gggCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCTCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAgcg                                                                >  1:312949/1‑140 (MQ=255)                                                                 ggCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCTCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAgcgc                                                               <  1:478146/140‑1 (MQ=255)                                                                     aCCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCTCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCgccagc                                                           <  2:1097477/140‑1 (MQ=255)                                                                     aCCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCTCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCgccagc                                                           <  2:1004260/140‑1 (MQ=255)                                                                      ccTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCTCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCgccagcc                                                          >  2:690833/1‑140 (MQ=255)                                                                      ccTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCTCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCgccagcc                                                          <  1:1150719/140‑1 (MQ=255)                                                                      ccTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCTCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCgccagcc                                                          <  2:544381/140‑1 (MQ=255)                                                                       ctgctgTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCTCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCgccagcca                                                         <  2:76623/140‑1 (MQ=255)                                                                           tgtgGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCTCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCgccagcca                                                         >  2:188707/1‑136 (MQ=255)                                                                           tgtgGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCTCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCgccagcca                                                         <  1:188707/136‑1 (MQ=255)                                                                            gtgGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCTCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGt                                                    >  2:569707/1‑140 (MQ=255)                                                                              gggCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCTCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTg                                                  >  1:776614/1‑140 (MQ=255)                                                                               ggCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCTCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCgccag                                                            >  2:21021/1‑129 (MQ=255)                                                                               ggCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCTCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCgccag                                                            <  1:21021/129‑1 (MQ=255)                                                                               ggCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCTCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGa                                                 <  2:1367172/140‑1 (MQ=255)                                                                                    aTGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCTCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATtctc                                            >  1:268025/1‑140 (MQ=255)                                                                                     tGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCTCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCg                                           <  1:690833/140‑1 (MQ=255)                                                                                             tCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCTCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCAtt                                   <  1:1120637/140‑1 (MQ=255)                                                                                                     cGGGTGGCGGAACGGCTGTATACCCATTACCACTGGCTCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACgg                           >  1:296652/1‑140 (MQ=255)                                                                                                        gTGGCGGAACGGCTGTATGCCCATTACCACTGGCTCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGct                        <  1:667280/140‑1 (MQ=255)                                                                                                         tGGCGGAACGGCTGTATGCCCATTACCACTGGCTCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGctg                       <  1:180118/140‑1 (MQ=255)                                                                                                                  ggCTGTATGCCCATTACCACTGGCTCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGctggaacctgga              <  2:827084/140‑1 (MQ=255)                                                                                                                         tGCCCATTACCACTGGCTCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGc       <  1:528862/140‑1 (MQ=255)                                                                                                                          gCCCATTACCACTGGCTCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCg      <  2:404775/140‑1 (MQ=255)                                                                                                                             cATTACCACTGGCTCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAAc   >  2:617396/1‑140 (MQ=255)                                                                                                                              aTTATCACTGGCTCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAAcc  >  1:219357/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                AATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACC  >  NC_000913/1035739‑1036003

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑