Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,358,793	A→G	V132A (GTG→GCG)	puuP ←	putrescine:H(+) symporter PuuP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,358,793	0	A	G	100.0%	127.5 / NA	39	V132A (GTG→GCG)	puuP	putrescine:H(+) symporter PuuP
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/21);  total (18/21)

GGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGG  >  NC_000913/1358659‑1358925                                                                                                                                       |                                                                                                                                     ggAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCGCTTcc                                                                                                                                 <  1:681536/140‑1 (MQ=255) ggAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAAAGGCGACGAAGGTTACCACCCCCACCCACGGCGGCGCTTcc                                                                                                                                 >  2:601250/1‑140 (MQ=255)      aTAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCGCTTCCGGGaa                                                                                                                            >  1:1329099/1‑140 (MQ=255)      aTAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCGCTTCCGGGaa                                                                                                                            <  2:1352819/140‑1 (MQ=255)             ccaccaTGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCGCTTCCGGGAAGAGGGCg                                                                                                                     <  2:984705/140‑1 (MQ=255)             ccaccaTGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCGCTTCCGGGAAGAGGGCg                                                                                                                     >  1:730932/1‑140 (MQ=255)              caccaTGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCGCTTCCGGGAAGAGGGCgg                                                                                                                    <  2:1329099/140‑1 (MQ=255)                ccaTGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCGCTTCCGGGAAGAGGGCgggg                                                                                                                  >  1:894405/1‑138 (MQ=255)                   tgatgGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCGCTTCCGGGAAGAGGGCGgagaga                                                                                                               >  2:719161/1‑140 (MQ=255)                   tgatgGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGGTACCACCCACACCCACGGCGGCGCTTCCGGGAAGAGGGCGgagaga                                                                                                               >  1:218285/1‑140 (MQ=255)                     atgGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAATGTTACCACCCACACCCACGGCGGCGCTTCCGGGAAGAGGGCGGAGagata                                                                                                             >  1:959834/1‑140 (MQ=255)                        gagaTTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCGCTTCCGGGAAGAGGGCGGAGagatagat                                                                                                          >  1:39593/1‑140 (MQ=255)                           aTTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTCAAAAGGACTCGAAGGATTACACCCACACCCCCGGCGGGGTTTTTTGGAAGATGGCGGAGAGAAAGAtttt                                                                                                       >  2:1111208/1‑140 (MQ=255)                             ttGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCGCTTCCGGGAAGAGGGCGGAGAGATAGATTTTCg                                                                                                     <  2:1163516/140‑1 (MQ=255)                                 aCCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCGCTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCcaa                                                                                                 <  2:39593/140‑1 (MQ=255)                                   cAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCGCTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCcaaca                                                                                               <  1:719161/140‑1 (MQ=255)                                   cAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCGCTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCcaaca                                                                                               <  1:1023023/140‑1 (MQ=255)                                                gTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCGCTTCCGGGAAGAGGGCGGGGAGATAGATTTTCGCCAACAAGACGTTAatcat                                                                                  >  1:3968/1‑140 (MQ=255)                                                gTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAAATGCGACGAAGGGTACCACCCACACCCACGGCGGCGCTTTCGGGGAGAGGGGGGGGGGATAAGTTTTTGCCCACAAGACGGTAatcaa                                                                                  >  2:318627/1‑139 (MQ=255)                                                   ttGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCGCTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCgg                                                                               >  1:227621/1‑140 (MQ=255)                                                         ttAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCGCTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCaaaaa                                                                         >  1:946633/1‑140 (MQ=255)                                                         ttAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCGCTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCaaaaa                                                                         >  1:897776/1‑140 (MQ=255)                                                           aGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCGCTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAaga                                                                       <  2:730932/140‑1 (MQ=255)                                                             cGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCGCTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAgaga                                                                     <  1:1105697/140‑1 (MQ=255)                                                                ccAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCGCTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATaa                                                                  <  1:1111208/140‑1 (MQ=255)                                                                              ttCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCGCTTCCGGGGAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGa                                                    >  1:252981/1‑140 (MQ=255)                                                                              ttCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCGCTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGa                                                    >  2:1025931/1‑140 (MQ=255)                                                                              ttCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCGCTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGa                                                    >  1:50099/1‑140 (MQ=255)                                                                              ttCAGATTCGCGGCGGTAAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCGCTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGa                                                    >  2:920167/1‑140 (MQ=255)                                                                                    ttCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCGCTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGcc                                              <  1:517089/140‑1 (MQ=255)                                                                                      cgcgGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCGCTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGa                                            >  1:384781/1‑140 (MQ=255)                                                                                        cggcggTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCGCTTCCGGGGAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGAACAGCCGAcc                                          >  2:517911/1‑140 (MQ=255)                                                                                                         acgaAGGTTACCACCCACACCCACGGCGGCGCTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCgg                         <  2:218285/140‑1 (MQ=255)                                                                                                                       ccacacccacGGCGGCGCTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACtttt           <  1:601250/140‑1 (MQ=255)                                                                                                                         acacccacGGCGGCGCTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTgc         >  2:300418/1‑140 (MQ=255)                                                                                                                         acacccacGGCGGCGCTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTgc         >  1:248657/1‑140 (MQ=255)                                                                                                                          cacccacGGCGGCGCTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTgcg        >  1:195148/1‑140 (MQ=255)                                                                                                                            cccacGGCGGCGCTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGcgcg      <  1:920167/140‑1 (MQ=255)                                                                                                                            cccacGGCGGCGCTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGcgcg      <  1:590278/140‑1 (MQ=255)                                                                                                                            cccacGGCGGCGCTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGcgcg      <  1:318627/140‑1 (MQ=255)                                                                                                                            cccacGGCGGCGCTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGcgcg      <  1:299356/140‑1 (MQ=255)                                                                                                                            cccacGGCGGCGCTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGcgcg      <  2:897776/140‑1 (MQ=255)                                                                                                                            cccacGGCGGCGCTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGcgcg      <  2:959834/140‑1 (MQ=255)                                                                                                                            cccacGGCGGCGCTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGcgcg      <  1:695686/140‑1 (MQ=255)                                                                                                                                cggcggCGCTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAgg  >  2:1081274/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                     GGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGG  >  NC_000913/1358659‑1358925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑