Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,360,535	A→G	V125A (GTC→GCC)	puuA ←	glutamate‑putrescine ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,360,535	0	A	G	100.0%	122.0 / NA	34	V125A (GTC→GCC)	puuA	glutamate‑putrescine ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (8/26);  total (8/26)

CGTCGCGCTGGCGATCCAGTAAATAGAACTCCAGCTCTACCGCTACGACCGGGAACAATCCGCGCTGGCGCAGCTGCTGCCACAGGCGGTTAAGAACGTTCCGCGGCTCAACGTCAAAGGGAGCGCCATCTTCATCGACCATGGTCAGGAGCATCTGACCGATAAACTCTGGATCGGCGGCAGAAGGAGTTAAGGAACCGAGAACAGGAACACAGGTACGATCCGGCTCGCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGC  >  NC_000913/1360398‑1360667                                                                                                                                          |                                                                                                                                     cGTCGCGCTGGCGATCCAGTAAATAGAACTCCAGCTCTACCGCTACGACCGGGAACAATCCGCGCTGGCGCAGCTGCTGCCACAGGCGGATAAGAACGTTCCCCGGCTCCACGTCAAATGGCGCGCCATCTTCATCGGcc                                                                                                                                    >  1:369655/1‑140 (MQ=255)  gTCGCGCTGGCGATCCAGTAAATAGAACTCCAGCTCTACCGCTACGACCGGGAACAATCCGCGCTGGCGCAGCTGCTGCCACAGGCGGTTAAGAACGTTCCGCGGCTCAACGTCAAAGGGAGCGCCATCTTCATCGGCCa                                                                                                                                   <  2:310451/140‑1 (MQ=255)    cgcgCTGGCGATCCAGTAAATAGAACTCCAGCTCTACCGCTACGACCGGGAACAATCCGCGCTGGCGCAGCTGCTGCCACAGGCGGTTAAGAACGTTCCGCGGCTCAACGTCCAAAGGAGCGCCATCTTCATCGGCCATg                                                                                                                                 >  2:1302210/1‑140 (MQ=255)     gcgcTGGCGATCCAGTAAATAGAACTCCAGCTCTACCGCTACGACCGGGAACAATCCGCGCTGGCGCAGCTGCTGCCACAGGCGGTTAAGAACGTTCCGCGGCTCAACGTCAAAGGGAGCGCCATCTTCATCGGCCATgg                                                                                                                                <  1:1158890/140‑1 (MQ=255)     gcgcTGGCGATCCAGTAAATAGAACTCCAGCTCTACCGCTACGACCGGGAACAATCCGCGCTGGCGCAGCTGCTGCCACAGGCGGTTAAGAACGTTCCGCGGCTCAACGTCAAAGGGAGCGCCATCTTCATCGGCCATgg                                                                                                                                <  1:437963/140‑1 (MQ=255)      cgcTGGCGATCCAGTAAATAGAACTCCAGCTCTACCGCTACGACCGGGAACAATCCGCGCTGGCGCAGCTGCTGCCACAGGCGGTTAAGAACGTTCCGCGGCTCAACGTCAAAGGGAGCGCCATCTTCATCGGcc                                                                                                                                    >  2:22671/1‑135 (MQ=255)       gcTGGCGATCCAGTAAATAGAACTCCAGCTCTACCGCTACGACCGGGAACAATCCGCGCTGGCGCAGCTGCTGCCACAGGCGGTTAAGAACGTTCCGCGGCTCAACGTCAAAGGGAGCGCCATCTTCATCGGCCATGGTc                                                                                                                              <  2:998251/140‑1 (MQ=255)         tGGCGATCCAGTAAATAGAACTCCAGCTCTACCGCTACGACCGGGAACAATCCGCGCTGGCGCAGCTGCTGCCACAGGCGGTTAAGAACGTTCCGCGGCTCAACGTCAAAGGGAGCGCCATCTTCATCGGCCATGGTCAg                                                                                                                            <  1:355566/140‑1 (MQ=255)         gagacagccAGTAAATAGAACTCCAGCTCTACCGCTACGACCGGGAACAATCCGCGCTGGCGCAGCTGCTGCCACAGGCGGTTAAGAACGTTCCGCGGCTCAACGTCAAAGGGAGCGCCATCTTCATCGGCCATGGTCAg                                                                                                                            <  1:799206/133‑1 (MQ=255)          ggCGATCCAGTAAATAGAACTCCAGCTCTACCGCTACGACCGGGAACAATCCGCGCTGGCGCAGCTGCTGCCACAGGCGGTTAAGAACGTTCCGCGGCTCAACGTCAAAGGGAGCGCCATCTTCATCGGCCATGGTCAgg                                                                                                                           <  2:589592/140‑1 (MQ=255)            cGATCCAGTAAATAGAACTCCAGCTCTACCGCTACGACCGGGAACAATCCGCGCTGGCGCAGCTGCTGCCACAGGCGGTTAAGAACGTTCCGCGGCTCAACGTCAAAGGGAGCGCCATCTTCATCGGCCATggcctgagc                                                                                                                         <  1:794243/140‑8 (MQ=255)                     aaaTAGAACTCCAGCTCTACCGCTACGACCGGGAACAATCCGCGCTGGCGCAGCTGCTGCCACAGGCGGTTAAGAACGTTCCGCGGCTCAACGTCAAAGGGAGCGCCATCTTCATCGGCCATGGTCAGGAGCATCTGAcc                                                                                                                >  2:1262997/1‑140 (MQ=255)                        tAGAACTCCAGCTCTACCGCTACGACCGGGAACAATCCGCGCTGGCGCAGCTGCTGCCACAGGCGGTTAAGAACGTTCCGCGGCTCAACGTCAAAGGGAGCGCCATCTTCATCGGCCATGGTCAGGAGCATCTGACCGAt                                                                                                             <  1:1116310/140‑1 (MQ=255)                        tAGAACTCCAGCTCTACCGCTACGACCGGGAACAATCCGCGCTGGCGCAGCTGCTGCCACAGGCGGTTAAGAACGTTCCGCGGCTCAACGTCAAAGGGAGCGCCATCTTCATCGGCCATGGTCAGGAGCATCTGACCGAt                                                                                                             <  1:195614/140‑1 (MQ=255)                        tAGAACTCCAGCTCTACCGCTACGACCGGGAACAATCCGCGCTGGCGCAGCTGCTGCCACAGGCGGTTAAGAACGTTCCGCGGCTCAACGTCAAAGGGAGCGCCATCTTCATCGGCCATGGTCAGGAGCATCTGACCGAt                                                                                                             <  1:1262997/140‑1 (MQ=255)                        tAGAACTCCAGCTCTACCGCTACGACCGGGAACAATCCGCGCTGGCGCAGCTGCTGCCACAGGCGGTTAAGAACGTTCCGCGGCTCAACGTCAAAGGGAGCGCCATCTTCATCGGCCATGGTCAGGAGCATCTGACCGAt                                                                                                             <  1:756868/140‑1 (MQ=255)                           aaCTCCAGCTCTACCGCTACGACCGGGAACAATCCGCGCTGGCGCAGCTGCTGCCACAGGCGGTTAAAAACGTTCCGCGGCTCAACCTCAAAGGGAGCGCCCTCTTCATCCGCCATGGTCAGGAGCAACTGAcctgtctc                                                                                                          >  2:799206/1‑134 (MQ=255)                           aaCTCCAGCTCTACCGCTACGACCGGGAACAATCCGCGCTGGCGCAGCTGCTGCCACAGGCGGCTAAGAACGTTTCCCGGGGCCACGTTAAAAGGGGCGCCATCTTCCTTGGCCCCGGGCAGGGGCAACCCACCGGTaaa                                                                                                          >  2:711510/1‑140 (MQ=255)                             cTCCAGCTCTACCGCTACGACCGGGAACAATCCGCGCTGGCGCAGCTGCTGCCACAGGCGGGTAAGAAAGTTCCCCGGCTCAACGTCAAAGGGAGCGCCATCTTCATCTGCCATGGTCAGGGGCAACTTACCGCTaaaac                                                                                                        >  2:1141697/1‑138 (MQ=255)                                         cGCTACGACCGGGAACAATCCGCGCTGGCGCAGCTGCTGCCACAGGCGGTTAAGAACGTTCCGCGGCTCAACGTCAAAGGGAGCGCCATCTTCATCGGCCATGGTCAGGAGCATCTGACCGATAAACTCTGGATcggcgg                                                                                            <  2:1209354/140‑1 (MQ=255)                                             aCGACCGGGAACAATCCGCGCTGGCGCAGCTGCTGCCACAGGCGGTTAAGAACGTTCCGCGGCTCAACGTCAAAGGGAGCGCCATCTTCATCGGCCATGGTCAGGAGCATCTGACCGATAAACTCTGGATCGGCGGCAGa                                                                                        <  2:1079506/140‑1 (MQ=255)                                              cGACCGGGAACAATCCGCGCTGGAGCAGCTGCTGCCACAGGCGGTTAAGAACGTACCGCGGCTCAACGTCAAAGGGCGCGACATCTTCATCGGCCATGGTCAGGAGCATCTGACCGCTGAACTCTGGATCGGCGGCAGaa                                                                                       <  2:943818/140‑1 (MQ=255)                                                  cGGGAACAATCCGCGCTGGCGCAGCTGCTGCCACAGGCGGTTAAGAACGTTCCGCGGCTCAACGTCAAAGGGAGCGCCATCTTCATCGGCCATGGTCAGGAGCATCTGACCGATAAACTCTGGATCGGCGGCAGAAGGAg                                                                                   <  2:584352/140‑1 (MQ=255)                                                   gggAACAATCCGCGCTGGCGCAGCTGCTGCCACAGGCGGTTAAGAACGTTCCGCGGCTCAACGTCAAAGGGAGCGCCATCTTCATCGGCCATGGTCAGGAGCATCTGACCGATAAACTCTGGATCGGCGGCAGAAGGAgg                                                                                  >  2:483571/1‑139 (MQ=255)                                                     gAACAATCCGCGCTGGCGCAGCTGCTGCCACAGGCGGTTAAGAACGTTCCGCGGCTCAACGTCAAAGGGAGCGCCATCTTCATCGGCCATGGTCAGGAGCATCTGACCGATAAACTCTGGATCGGCGGCAGAAGGAGTTa                                                                                <  2:1126980/140‑1 (MQ=255)                                                                   ggCGCAGCTGCTGCCACAGGCGGTTAAGAACGTTCCGCGGCTCAACGTCAAAGGGAGCGCCCTCTTCATCGGCCCTGGTCAGGAGCATCTGACCGATAAAATCTGGATCGGCGGGAGAAGGGGGTAAAGAAACCAGaaaa                                                                  >  2:56072/1‑138 (MQ=255)                                                                       cAGCTGCTGCCACAGGCGGTTAAGAACGTTCCGCGGCTCAACGTCAAAGGGAGCGCCATCTTCATCGGCCATGGTCAGGAGCATCTGACCGATAAACTCTGGATCGGCGGCAGAAGGAGTTAAGGAACCGAGAACAGGaa                                                              <  2:594376/140‑1 (MQ=255)                                                                                    aGGCGGTTAAGAAAGTTCCGCGGCTCAACGTCAAAGGGGGCGCCATCTTCATCGGCCATGGTCAGGGGCATCTGACCGATAAAATCTGGGTCGGCGGCAGAAGGGGGTAAAGAAACGAGAACAGGAACACAAGGACGaac                                                 >  2:1261001/1‑138 (MQ=255)                                                                                       cGGTTAAGAACGTTCCGCGGCTCAACGTCAAAGGGAGCGCCATCTTCATCGGCCATGGTCAGGAGCATCTGACCGATAAACTCTGGATCGGCGGCAGAAGGAGTTAAGGAACCGAGAACAGGAACACAGGTACGATCCgg                                              <  1:247036/140‑1 (MQ=255)                                                                                       cGGTTAAGAAAGTTCCGCGGCTCCACGTCAAAGGGAGCGCCATCTTCATCGGCCATGGTCAGGGGCATCTGACCGATAAAATCTGGATCGGCGGGAGGAAGAGGTTAGGAAACCAGAAAAGGAAAACAGGGACGATcccg                                              >  2:11786/1‑138 (MQ=255)                                                                                                      cgcgGCTCAACGTCAAAGGGAGCGCCATCTTCATCGGCCATGGTCAGGAGCATCTGACCGATAAACTCTGGATCGGCGGCAGAAGGAGTTAAGGAACCGAGAACAGGAACACAGGTACGATCCGGCTCGCCCATTTCCTg                               >  1:1163024/1‑140 (MQ=255)                                                                                                      cgcgGCTCAACGTCAAAGGGAGCGCCATCTTCATCGGCCATGGTCAGGAGCATCTGACCGATAAACTCTGGATCGGCGGCAGAAGGAGTTAAGGAACCGAGAACAGGAACACAGGTACGATCCGGCTCGCCCATTTCCTg                               >  1:796815/1‑140 (MQ=255)                                                                                                         ggCTCAACGTCAAAGGGAGCGCCATCTTCATCGGCCATGGTCAGGAGCATCTGACCGATAAACTCTGGATCGGCGGCAGAAGGAGTTAAGGAACCGAGAACAGGAACACAGGTACGATCCGGCTCGCCCATTTCCTGAcc                            <  2:292791/140‑1 (MQ=255)                                                                                                              aaCGTCAAAGGGAGCGCCATCTTCATCGGCCATGGTCAGGAGCATCTGACCGATAAACTCTGGATCGGCGGCAGAAGGAGTTAAGGAACCGAGAACAGGAACACAGGTACGATCCGGCTCGCCCATTTCCTGACCCAGAc                       >  2:1260414/1‑140 (MQ=255)                                                                                                                cGTCAAAGGGAGCGCCATCTTCATCGGCCATGGTCAGGAGCATCTGACCGATAAACTCTGGATCGGCGGCAGAAGGAGTTAAGGAACCGAGAACAGGAACACAGGTACGATCCGGCTCGCCCATTTCCTGACCCAGACCt                     <  2:265363/140‑1 (MQ=255)                                                                                                                   cAAAGGGAGCGCCATCTTCATCGGCCATGGTCAGGAGCATCTGACCGATAAACTCTGGATCGGCGGCAGAAGGAGTTAAGGAACCGAGAACAGGAACACAGGTACGATCCGGCTCGCCCATTTCCTGACCCAGACCTGct                  <  1:11786/140‑1 (MQ=255)                                                                                                                     aaGGGAGCGCCATCTTCATCGGCCATGGTCAGGAGCATCTGACCGATAAACTCTGGATCGGCGGCAGAAGGAGTTAAGGAACCGAGAACAGGAACACAGGTACGATCCGGCTCGCCCATTTCCTGACCCAGACCTGcttc                <  1:1260414/140‑1 (MQ=255)                                                                                                                          aGCGCCATCTTCATCGGCCATGGTCAGGAGCATCTGACCGATAAACTCTGGATCGGCGGCAGAAGGAGTTAAGGAACCGAGAACAGGAACACAGGTACGATCCGGCTCGCCCATTTCCTGACCCAGAcc                      <  2:1194179/129‑1 (MQ=255)                                                                                                                          aGCGCCATCTTCATCGGCCATGGTCAGGAGCATCTGACCGATAAACTCTGGATCGGCGGCAGAAGGAGTTAAGGAACCGAGAACAGGAACACAGGTACGATCCGGCTCGCCCATTTCCTGACCCAGAcc                      >  1:1194179/1‑129 (MQ=255)                                                                                                                          aGCGCCATCTTCATCGGCCATGGTCAGGAGCATCTGACCGATAAACTCTGGATCGGCGGCAGAAGGAGTTAAGGAACCGAGAACAGGAACACAGGTACGATCCGGCTCGCCCATTTCCTGACCCAGACCTGCTTCTTcta           >  2:1025299/1‑140 (MQ=255)                                                                                                                          aGCGCCATCTTCATCGGCCATGGTCAGGAGCATCTGACCGATAAACTCTGGATCGGCGGCAGAAGGAGTTAAGGAACCGAGAACAGGAACACAGGTACGATCCGGCTCGCCCATTTCCTGACCCAGACCTGCTTCTTcta           <  1:711510/140‑1 (MQ=255)                                                                                                                          aGCGCCATCTTCATCGGCCATGGTCAGGAGCATCTGACCGATAAACTCTGGATCGGCGGCAGAAGGAGTTAAGGAACCGAGAACAGGAACACAGGTACGATCCGGCTCGCCCATTTCCTGACCCAGACCTGCTTCTTcta           >  1:326109/1‑140 (MQ=255)                                                                                                                           gcgcCATCTTCATCGGCCATGGTCAGGAGCATCTGACCGATAAACTCTGGATCGGCGGCAGAAGGAGTTAAGGAACCGAGAACAGGAACACAGGTACGATCCGGCTCGCCCATTTCCTGACCCAGACCTGCTTCTTctac          >  1:1348971/1‑140 (MQ=255)                                                                                                                                   ttCATCGGCCATGGTCAGGAGCATCTGACCGATAAACTCTGGATCGGCGGCAGAAGGAGTTAAGGAACCGAGAACAGGAACACAGGTACGATCCGGCTCGCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGc  <  1:1302210/140‑1 (MQ=255)                                                                                                                                   ttCATCGGCCATGGTCAGGAGCATCTGACCGATAAACTCTGGATCGGCGGCAGAAGGAGTTAAGGAACCGAGAACAGGAACACAGGTACGATCCGGCTCGCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGc  <  1:56072/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                     CGTCGCGCTGGCGATCCAGTAAATAGAACTCCAGCTCTACCGCTACGACCGGGAACAATCCGCGCTGGCGCAGCTGCTGCCACAGGCGGTTAAGAACGTTCCGCGGCTCAACGTCAAAGGGAGCGCCATCTTCATCGACCATGGTCAGGAGCATCTGACCGATAAACTCTGGATCGGCGGCAGAAGGAGTTAAGGAACCGAGAACAGGAACACAGGTACGATCCGGCTCGCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGC  >  NC_000913/1360398‑1360667

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑