Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	220,088	A→G	intergenic (‑93/+25)	rcsF ← / ← metQ	outer membrane lipoprotein RcsF/L‑methionine/D‑methionine ABC transporter membrane anchored binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	220,088	0	A	G	100.0%	198.6 / NA	59	intergenic (‑93/+25)	rcsF/metQ	outer membrane lipoprotein RcsF/L‑methionine/D‑methionine ABC transporter membrane anchored binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (38/21);  total (38/21)

CCGCTTAGCATGAGTGCTACTAAACAGATCGGTAAAGCACGCATAGTATTTCCTCAATGTATGATCAAAACGTCAATATTGAATCAGGAGCTTGTAAAAATGACAAGACGGGCAAGCGCCCGTCCTGAATGATATTACAAATTGTGGAAACAGCCTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATC  >  NC_000913/219952‑220223                                                                                                                                         |                                                                                                                                        ccGCTTAGCATGAGTGCTACTAAACAGATCTGTAAAGCACGCATAGTATTTCCTCAATGTATGATCAAACCGTCAATATTGAATCAGGGGCTTGTAAAAATGACAAGACGGGCAAGCGCCCGTCCTGAATGATATTGCaa                                                                                                                                      <  2:1316414/140‑1 (MQ=255) ccGCTTAGCATGAGTGCTACTAAACAGATCGGTAAAGCACGCATAGTATTTCCTCAATGTATGATCAAAACGTCAATATTGAATCAGGAGCTTGTAAAAATGACAAGACGGGCAAGCGCCCGTCCTGAATGATATTGCaa                                                                                                                                      <  2:630356/140‑1 (MQ=255) ccGCTTAGCATGAGTGCTACTAAACAGATCGGTAAAGCACGCATAGTATTTCCTCAATGTATGATCAAAACGTCAATATTGAATCAGGAGCTTGTAAAAATGACAAGACGGGCAAGCGCCCGTCCTGAATGATATTGCaa                                                                                                                                      <  1:263717/140‑1 (MQ=255) ccGCTTAGCATGAGTGCTACTAAACAGATCGGTAAAGCACGCATAGTATTTCCTCAATGTATGATCAAAACGTCAATATTGAATCAGGAGCTTGTAAAAATGACAAGACGGGCAAGCGCCCGTCCTGAATGATATTGCaa                                                                                                                                      >  2:1316720/1‑140 (MQ=255) ccGCTTAGCATGAGTGCTACTAAACAGATCGGTAAAGCACGCATAGTATTTCCTCAATGTATGATCAAAACGTCAATATTGAATCAGGAGCTTGTAAAAATGACAAGACGGGCAAGCGCCCGTCCTGAATGATATTGCaa                                                                                                                                      <  1:1316720/140‑1 (MQ=255) ccGCTTAGCATGAATGCTACTAAACAGATCGGTAAAGCACGCATAGTATTTCCTCAATGTATGATCAAAACGTCAATATTGAATCAGGAGCTTGTAAAAATGACAAGACGGGCAAGCGCCCGTCCTGAATGATATTGCaa                                                                                                                                      <  1:1271417/140‑1 (MQ=255)                gCTACTAAACAGATCGGTAAAGCACGCATAGTATTTCCTCAATGTATGATCAAAACGTCAATATTGAATCAGGAGCTTGTAAAAATGACAAGACGGGCAAGCGCCCGTCCTGAATAATATTGCAAATTGTGGAAACAGcc                                                                                                                       <  2:1265610/140‑1 (MQ=255)                       aaCAGATCGGTAAAGCACGCATAGTATTTCCTCAATGTATGATCAAAACGTCAATATTGAATCAGGAGCTTGTAAAAATGACAAGACGGGCAAGCGCCCGTCCTGAATGATATTGCAAATTGTGGAAACAGCCTAAAAAt                                                                                                                >  2:108328/1‑140 (MQ=255)                         cAGATCGGTAAAGCACGCATAGTATTTCCTCAATGTATGATCAAAACGTCAATATTGAATCAGGAGCTTGTAAAAATGACAAGACGGGCAAGCGCCCGTCCTGAATGATATTGCAAATTGTGGAAACAGCCTAAAAATTa                                                                                                              >  1:1329969/1‑140 (MQ=255)                           gATCGGTAAAGCACGCATAGTATTTCCTCAATGTATGATCAAAACGTCAATATTGAATCAGGAGCTTGTAAAAATGACAAGACGGGCAAGCGCCCGTCCTGAATGATATTGCAAATTGTGGAAACAGCCTAAAAATTAcc                                                                                                            >  2:33857/1‑140 (MQ=255)                            aTCGGTAAAGCACGCATAGTATTTCCTCAATGTATGATCAAAACGTCAATATTGAATCAGGAGCTTGTAAAAATGACAAGACGGGCAAGCGCCCGTCCTGAATGATATTGCAAATTGTGGAAACAGCCTAAAAATTAc                                                                                                             >  2:1064770/1‑138 (MQ=255)                            aTCGGTAAAGCACGCATAGTATTTCCTCAATGTATGATCAAAACGTCAATATTGAATCAGGAGCTTGTAAAAATGACAAGACGGGCAAGCGCCCGTCCTGAATGATATTGCAAATTGTGGAAACAGCCTAAAAATTAc                                                                                                             <  1:1064770/138‑1 (MQ=255)                            aTCGGTAAAGCACGCATAGTATTTCCTCAATGTATGATCAAAACGTCAATATTGAATCAGGAGCTTGTAAAAATGACAAGACGGGCAAGCGCCCGTCCTGAATGATATTGCAAATTGTGGAAACAGCCTAAAAATTACCa                                                                                                           >  2:1153544/1‑140 (MQ=255)                            aTCGGTAAAGCACGCATAGTATTTCCTCAATGTATGATCAAAACGTCAATATTGAATCAGGAGCTTGTAAAAATGACAAGACGGGCAAGCGCCCGTCCTGAATGATATTGCAAATTGTGGAAACAGCCTAAAAATTACCa                                                                                                           >  2:631691/1‑140 (MQ=255)                            aTCGGTAAAGCACGCATAGTATTTCCTCAATGTATGATCAAAACGTCAATATTGAATCAGGAGCTTGTAAAAATGACAAGACGGGCAAGCGCCCGTCCTGAATGATATTGCAAATTGTGGAAACAGCCTAAAAATTACCa                                                                                                           <  2:411292/140‑1 (MQ=255)                              cGGTAAAGCACGCATAGTATTTCCTCAATGTATGATCAAAACGTCAATATTGAATCAGGAGCTTGTAAAAATGACAAGACGGGCAAGCGCCCGTCCTGAATGATATTGCAAATTGTGGAAACAGCCTAAGAATTACCAGc                                                                                                         >  2:860694/1‑140 (MQ=255)                              cGGTAAAGCACGCATAGTATTTCCTCAATGTATGATCAAAACGTCAATATTGAATCAGGAGCTTGTAAAAATGACAAGACGGGCAAGCGCCCGTCCTGAATGATATTGCAAATTGTGGAAACAGCCTAAAAATTACCAGc                                                                                                         >  2:1265437/1‑140 (MQ=255)                                    aGCACGCATAGTATTTCCTCAATGTATGATCAAAACGTCAATATTGAATCAGGAGCTTGTAAAAATGACAAGACGGGCAAGCGCCCGTCCTGAATGATATTGCAAATTGTGGAAACAGCCTAAAAATTACCAGCCTTTaa                                                                                                   <  1:860694/140‑1 (MQ=255)                                      cACGCATAGTATTTCCTCAATGTATGATCAAAACGTCAATATTGAATCAGGAGCTTGTAAAAATGACAAGACGGGCAAGCGCCCGTCCTGAATGATATTGCAAATTGTGGAAACAGCCTAAAAATTACCAGCCTTTAACa                                                                                                 >  1:606098/1‑140 (MQ=255)                                      cACGCATAGTATTTCCTCAATGTATGATCAAAACGTCAATATTGAATCAGGAGCTTGTAAAAATGACAAGACGGGCAAGCGCCCGTCCTGAATGATATTGCAAATTGTGGAAACAGCCTAAAAATTACCAGCCTTTAACa                                                                                                 >  2:118318/1‑140 (MQ=255)                                          cATAGTATTTCCTCAATGTATGATCAAAACGTCAATATTGAATCAGGAGCTTGTAAAAATGACAAGACGGGCAAGCGCCCGTCCTGAATGATATTGCAAATTGTGGAAACAGCCTAAAAATTACCAGCCTTTAACAGCTc                                                                                             <  1:33857/140‑1 (MQ=255)                                                 tttCCTCAATGTATGATCAAAACGTCAATATTGAATCAGGAGCTTGTAAAAATGACAAGACGGGCAAGCGCCCGTCCTGAATGATATTGCAAATTGTGGAAACAGCCTAAAAATTACCACCCTTTAACAGCTCCGCCGtt                                                                                      <  1:1046104/140‑1 (MQ=255)                                                       cAATGTATGATCAAAACGTCAATATTGAATCAGGAGCTTGTAAAAATGACAAGACGGGCAAGCGCCCGTCCTGAATGATATTGCAAATTGTGGAAACAGCCTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAacac                                                                                >  2:85245/1‑140 (MQ=255)                                                         aTGTATGATCAAAACGTCAATATTGAATCAGGAGCTTGTAAAAATGACAAGACGGGCAAGCGCCCGTCCTGAATGATATTGCAAATTGTGGAAACAGCCTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACACtt                                                                              >  1:427953/1‑140 (MQ=255)                                                         aTGTATGATCAAAACGTCAATATTGAATCAGGAGCTTGTAAAAATGACAAGACGGGCAAGCGCCCGTCCTGAATGATATTGCAAATTGTGGAAACAGCCTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACACtt                                                                              >  2:1166030/1‑140 (MQ=255)                                                              tGATCAAAACGTCAATATTGAATCAGGAGCTTGTAAAAATGACAAGACGGGCAAGCGCCCGTCCTGAATGATATTGCAAATTGTGGAAACAGCCTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACACtttgttt                                                                         >  1:1331481/1‑140 (MQ=255)                                                               gATCAAAACGTCAATATTGAATCAGGAGCTTGTAAAAATGACAAGACGGGCAAGCGCCCGTCCTGAATGATATTGCAAATTGTGGAAACAGCCTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACACtttgtttg                                                                        >  1:754306/1‑140 (MQ=255)                                                                 tCAAAACGTCAATATTGAATCAGGAGCTTGTAAAAATGACAAGACGGGCAAGCGCCCGTCCTGAATGATATTGCAAATTGTGGAAACAGCCTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTtgct                                                                      <  1:631691/140‑1 (MQ=255)                                                                 acAAAACGTCAATATTGAATCAGGAGTTTGAAAAAATGACAAGCCGGGCAAGCGCCCGTCCTAAATGATATTGAAAATTGTGAAAACAGCCTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTtgct                                                                      <  2:1331481/139‑1 (MQ=255)                                                                 acAAAACGTCAATATTGAATCAGGAGCTTGTAAAAATGACAAGACGGGCAAGCGCCCGTCCTGAATGATATTGCAAATTGTGGAAACAGCCTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTtgct                                                                      <  2:838044/139‑1 (MQ=255)                                                                   aaaaCGTCAATATTAAATCAGGAGTTTGAAAAAATGACAAGACGGGCAAGCCCCCGTCCTGAATGATATTGCAAATTGTGAAAACAGCCTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTtgctgc                                                                    <  2:890547/140‑1 (MQ=255)                                                                    aaaCGTCAATATTGAATCAGGAGCTTGTAAAAATGACAAGACGGGCAAGCGCCCGTCCTGAATGATATTGCAAATTGTGGAAACAGCCTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTgctgct                                                                   >  1:1251451/1‑140 (MQ=255)                                                                    aaaCGTCAATATTGAATCAGGAGCTTGTAAAAATGACAAGACGGGCAAGCGCCCGTCCTGAATGATATTGCAAATTGTGGAAACAGCCTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTgctgct                                                                   >  2:1013349/1‑140 (MQ=255)                                                                    aaaCGTCAATATTGAATCAGGAGCTTGTAAAAATGACAAGACGGGCAAGCGCCCGTCCTGAATGATATTGCAAATTGTGGAAACAGCCTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTgctgct                                                                   >  2:262290/1‑140 (MQ=255)                                                                    aaaCGTCAATATTGAATCAGGAGCTTGTAAAAATGACAAGACGGGCAAGCGCCCGTCCTGAATGATATTGCAAATTGTGAAAACAGCCTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTgctgct                                                                   >  1:1101293/1‑140 (MQ=255)                                                                      aCGTCAATATTGAATCAGGAGCTTGTAAAAATGACAAGACGGGCAAGCGCCCGTCCTGAATGATATTGCAAATTGTGGAAACAGCCTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTc                                                                 >  2:155675/1‑140 (MQ=255)                                                                       cGTCAATATTGAATCAGGAGCTTGTAAAAATGACAAGACGGGCAAGCGCCCGTCCTGAATGATATTGCAAATTGTGGAAACAGCCTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACGCTTTGTTTGCTGCTTCg                                                                <  1:1013349/140‑1 (MQ=255)                                                                        gTCAATATTGAATCAGGAGCTTGTAAAAATGACAAGACGGGCAAGCGCCCGTCCTGAATGATATTGCAAATTGTGGAAACAGCCTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGt                                                               >  1:988814/1‑140 (MQ=255)                                                                                  aaTCAGGAGCTTGTAAAAATGACAAGACGGGCAAGCGCCCGTCCTGAATGATATTGCAAATTGTGGAAACAGCCTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTc                                                     >  1:764586/1‑140 (MQ=255)                                                                                    tCAGGAGCTTGTAAAAATGACAAGACGGGCAAGCGCCCGTCCTGAATGATATTGCAAATTGTGGAAACAGCCTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAg                                                   <  1:566963/140‑1 (MQ=255)                                                                                    tCAGGAGCTTGTAAAAATGACAAGACGGGCAAGCGCCCGTCCTGAATGATATTGCAAATTGTGGAAACAGCCTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAg                                                   <  1:262290/140‑1 (MQ=255)                                                                                    acAGGAGTTTGTAAAAATGACAAGAGGGACAAGCGCCCGTCCTAAATGATATTGAAAATTGTGGAAACACCCAAAAAATTACCACCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAg                                                   <  2:691690/139‑1 (MQ=255)                                                                                       ggAGCTTGTAAAAATGACAAGACGGGCAAGCGCCCGTCCTGAATGATATTGCAAATTGTGGAAACAGCCTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACt                                                >  1:691690/1‑140 (MQ=255)                                                                                       ggAGCTTGTAAAAATGACAAGACGGGCAAGCGCCCGTCCTGAATGATATTGCAAATTGTGGAAACAGCCTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACt                                                >  2:324406/1‑140 (MQ=255)                                                                                       ggAGCTTGTAAAAATGACAAGACGGGCAAGCGCCCGTCCTGAATGATATTGCAAATTGTGGAAACAGCCTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACt                                                >  1:240737/1‑140 (MQ=255)                                                                                       ggAGCTTGTAAAAATGACAAGACGGGCAAGCGCCCGTCCTGAATGATATTGCAAATTGTGGAAACAGCCTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACt                                                >  1:1284317/1‑140 (MQ=255)                                                                                       ggAGCTTGTAAAAATGACAAGACGGGCAAGCGCCCGTCCTGAATGATATTGCAAATTGTGGAAACAGCCTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACt                                                >  2:960418/1‑140 (MQ=255)                                                                                        gAGCTTGTAAAAATGACAAGACGGGCAAGCGCCCGTCCTGAATGATATTGCAAATTGTGGAAACAGCCTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACt                                                          >  1:1023719/1‑129 (MQ=255)                                                                                        gAGCTTGTAAAAATGACAAGACGGGCAAGCGCCCGTCCTGAATGATATTGCAAATTGTGGAAACAGCCTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACt                                                          <  2:1023719/129‑1 (MQ=255)                                                                                         aGCTTGTAAAAATGACAAGACGGGCAAGCGCCCGTCCTGAATGATATTGCAAATTGTGGAAACAGCCTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCTTAAACTTCGTCAGACTGa                                              >  1:1054959/1‑140 (MQ=255)                                                                                         aGCTTGTAAAAATGACAAGACGGGCAAGCGCCCGTCCTGAATGATATTGCAAATTGTGGAAACAGCCTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGa                                              <  1:108328/140‑1 (MQ=255)                                                                                                   aaTGAAAAGCCGGACAAGCGCCCTTCCTAAATAATATTGCAAATTGTGGAAACAGCCTGAAAATTCCCACCTTTTATCAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGa                                    <  2:1223608/140‑1 (MQ=255)                                                                                                           gACGGGCAAGCGCCCGTCCTGAATGATATTGCAAATTGTGGAAACAGCCTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATttc                            <  2:988814/140‑1 (MQ=255)                                                                                                                   aGCGCCCGTCCTGAATGATATTGCAAATTGTGGAAACAGCCTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGtt                    >  1:95214/1‑140 (MQ=255)                                                                                                                      gcCCGTCCTGAATGATATTGCAAATTGTGGAAACAGCCTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTtctc                 >  2:961263/1‑140 (MQ=255)                                                                                                                          gTCCTGAATGATATTGCAAATTGTGGAAACAGCCTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAAtt                              >  2:167808/1‑123 (MQ=255)                                                                                                                          gTCCTGAATGATATTGCAAATTGTGGAAACAGCCTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAAtt                              <  1:167808/123‑1 (MQ=255)                                                                                                                          gTCCTGAATGATATTGCAAATTGTGGAAACAGCCTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCg             >  1:319054/1‑140 (MQ=255)                                                                                                                          gTCCTGAATGATATTGCAAATTGTGGAAACAGCCTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCg             <  1:961263/140‑1 (MQ=255)                                                                                                                           tCCTGAATGATATTGCAAATTGTGGAAACAGCCTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGt            >  1:30995/1‑140 (MQ=255)                                                                                                                           tCCTGAATGATATTGCAAATTGTGGAAACAGCCTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGt            >  1:389483/1‑140 (MQ=255)                                                                                                                              tGAATGATATTGCAAATTGTGGAAACAGCCTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCtt         >  2:156420/1‑140 (MQ=255)                                                                                                                                aaTGATATTGCAAATTGTGGAAACAGCCTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTg       <  2:754306/140‑1 (MQ=255)                                                                                                                                     tatTGCAAATTGTGGAAACAGCCTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATc  >  1:230204/1‑140 (MQ=255)                                                                                                                                      atTGCAAATTGTGGAAACAGCCTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCg             <  2:1370841/128‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        CCGCTTAGCATGAGTGCTACTAAACAGATCGGTAAAGCACGCATAGTATTTCCTCAATGTATGATCAAAACGTCAATATTGAATCAGGAGCTTGTAAAAATGACAAGACGGGCAAGCGCCCGTCCTGAATGATATTACAAATTGTGGAAACAGCCTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATC  >  NC_000913/219952‑220223

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑