Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,613,652	A→G	V351A (GTT→GCT)	sad ←	NAD(+)‑dependent succinate semialdehyde dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,613,652	0	A	G	100.0%	236.6 / NA	65	V351A (GTT→GCT)	sad	NAD(+)‑dependent succinate semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/38);  total (27/38)

GGTCGCTGAAAGGCCGAACTCACTATCATTAGCCAGTTCCAGTGCATGTTCTGCATCTTTCGCAATGGTGATTGCCGCAACGGGGCCAAACATTTCTTCCCGAAACGCGGTCATTTCTGGGGTAACATTCGCCAGAACCGTTGGCGGATAGTAGTTACCTGCCCCAGCCATCTTTTCCCCGCCCAGTAACAAACGCGCACCCTGCGCCAGGGTTTTCTCCACCTGATGATGCAGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGA  >  NC_000913/1613516‑1613786                                                                                                                                         |                                                                                                                                       ggTCGCTGAAAGGCCGAACTCACTATCATTAGCCAGTTCCAGTGCATGTTCTGCATCTTTCGCAATGGTGATTGCCGCAACGGGGCCAAACATTTCTTCCCGAAACGCGGTCATTTCTGGGGTAACATTCGCCAGAGCCg                                                                                                                                     <  2:107992/140‑1 (MQ=255)  gTCGCTGAAAGGCCGAACTCACTATCATTAGCCAGTTCCAGTGCATGTTCTGCATCTTTCGCAATGGTGATTGCCGCAACGGGGCCAAACATTTCTTCCCGAAACGCGGTCATTTCTGGGGTAACATTCGCCAGAGCCGt                                                                                                                                    >  2:200123/1‑140 (MQ=255)   tCGCTGAAAGGCCGAACTCACTATCATTAGCCAGTTCCAGTGCATGTTCTGCATCTTTCGCAATGGTGATTGCCGCAACGGGGCCAAACATTTCTTCCCGAAACGCGGTCATTTCTGGGGTAACATTCGCCAGAGCCGtt                                                                                                                                   >  1:995650/1‑140 (MQ=255)    cGCTGAAAGGCCGAACTCACTATCATTAGCCAGTTCCAGTGCATGTTCTGCATCTTTCGCAATGGTGATTGCCGCAACGGGGCCAAACATTTCTTCCCGAAACGCGGTCATTTCTGGGGTAACATTCGCCAGAGCCGTTg                                                                                                                                  <  1:1156428/140‑1 (MQ=255)    cGCTGAAAGGCCGAACTCACTATCATTAGCCAGTTCCAGTGCATGTTCTGCATCTTTCGCAATGGTGATTGCCGCAACGGGGCCAAACATTTCTTCCCGAAACGCGGTCATTTCTGGGGTAACATTCGCCAGAGCCGTTg                                                                                                                                  <  1:58316/140‑1 (MQ=255)         aaaGGCCGAACTCACTATCATTAGCCAGTTCCAGTGCATGTTCTGCATCTTTCGCAATGGTGATTGCCGCAACGGGGCCAAACATTTCTTCCCGAAACGCGGTCATTTCTGGGGTAACATTCGCCAGAGCCGTTGGCGGa                                                                                                                             <  1:1012404/140‑1 (MQ=255)         aaaGGCCGAACTCACTATCATTAGCCAGTTCCAGTGCATGTTCTGCATCTTTCGCAATGGTGATTGCCGCAACGGGGCCAAACATTTCTTCCCGAAACGCGGTCATTTCTGGGGTAACATTCGCCAGAGCCGTTGGCGGa                                                                                                                             <  2:343551/140‑1 (MQ=255)         aaaGGCCGAACTCACTATCATTAGCCAGTTCCAGTGCATGTTCTGCATCTTTCGCAATGGTGATTGCCGCAACGGGGCCAAACATTTCTTCCCGAAACGCGGTCATTTCTGGGGTAACATTCGCCAGAGCCGTTGGCGGa                                                                                                                             <  1:235717/140‑1 (MQ=255)         aaaGGCCGAACTCACTATCATTAGCCAGTTCCAGTGCATGTTCTGCATCTTTCGCAATGGTGATTGCCGCAACGGGGCCAAACATTTCTTCCCGAAACGCGGTCATTTCTGGGGTAACATTCGCCAGAGCCGTTGGCGGa                                                                                                                             <  1:1192908/140‑1 (MQ=255)              ccGAACTCACTATCATTAGCCAGTTCCAGTGCATGTTCTGCATCTTTCGCAATGGTGATTGCCGCAACGGGGCCAAACATTTCTTCCCGAAACGCGGTCATTTCTGGGGTAACATTCGCCAGAGCCGTTGGCGGAtagta                                                                                                                        <  1:1149005/140‑1 (MQ=255)                   cTCACTATCATTAGCCAGTTCCAGTGCATGTTCTGCATCTTTCGCAATGGTGATTGCCGCAACGGGGCCAAACATTTCTTCCCGAAACGCGGTCATTTCTGGGGTAACATTCGCCAGAGCCGTTGGCGGATAGTAGTTAc                                                                                                                   <  2:178554/140‑1 (MQ=255)                   cTCACTATCATTAGCCAGTTCCAGTGCATGTTCTGCATCTTTCGCAATGGTGATTGCCGCAACGGGGCCAAACATTTCTTCCCGAAACGCGGTCATTTCTGGGGTAACATTCGCCAGAGCCGTTGGCGGATAGTAGTTAc                                                                                                                   >  1:1247571/1‑140 (MQ=255)                   cTCACTATCATTAGCCAGTTCCAGTGCATGTTCTGCATCTTTCGCAATGGTGATTGCCGCAACGGGGCCAAACATTTCTTCCCGAAACGCGGTCATTTCTGGGGTAACATTCGCCAGAGCCGTTGGCGGATAGTAGTTAc                                                                                                                   <  1:334672/140‑1 (MQ=255)                           cATTAGCCAGTTCCAGTGCATGTTCTGCATCTTTCGCAATGGTGATTGCCGCAACGGGGCCAAACATTTCTTCCCGAAACGCGGTCATTTCTGGGGTAACATTCGCCAGAGCCGTTGGCGGATAGTAGTTACCTGCCCCa                                                                                                           <  1:200123/140‑1 (MQ=255)                           cATTAGCCAGTTCCAGTGCATGTTCTGCATCTTTCGCAATGGTGATTGCCGCAACGGGGCCAAACATTTCTTCCCGAAACGCGGTCATTTCTGGGGTAACATTCGCCAGAGCCGTTGGCGGATAGTAGTTACCTGCCCCa                                                                                                           <  2:445976/140‑1 (MQ=255)                                gCCAGTTCCAGTGCATGTTCTGCATCTTTCGCAATGGTGATTGCCGCAACGGGGCCAAACATTTCTTCCCGAAACGCGGTCATTTCTGGGGTAACATTCGCCAGAGCCGTTGGCGGATAGTAGTTACCTGCCCCAGCCAt                                                                                                      >  2:417293/1‑140 (MQ=255)                                     ttCCAGTGCATGTTCTGCATCTTTCGCAATGGTGATTGCCGCAACGGGGCCAAACATTTCTTCCCGAAACGCGGTCATTTCTGGGGTAACATTCGCCAGAGCCGTTGGCGGATAGTAGTTACCTGc                                                                                                               >  2:404884/1‑126 (MQ=255)                                     ttCCAGTGCATGTTCTGCATCTTTCGCAATGGTGATTGCCGCAACGGGGCCAAACATTTCTTCCCGAAACGCGGTCATTTCTGGGGTAACATTCGCCAGAGCCGTTGGCGGATAGTAGTTACCTGc                                                                                                               <  1:404884/126‑1 (MQ=255)                                     ttCCAGTGCATGTTCTGCATCTTTCGCAATGGTGATTGCCGCAACGGGGCCAAACATTTCTTCCCGAAACGCGGTCATTTCTGGGGTAACATTCGCCAGAGCCGTTGGCGGATAGTAGTTACCTGCCCCa                                                                                                           >  2:1076688/1‑130 (MQ=255)                                     ttCCAGTGCATGTTCTGCATCTTTCGCAATGGTGATTGCCGCAACGGGGCCAAACATTTCTTCCCGAAACGCGGTCATTTCTGGGGTAACATTCGCCAGAGCCGTTGGCGGATAGTAGTTACCTGCCCCa                                                                                                           <  1:1076688/130‑1 (MQ=255)                                     ttCCAGTGCATGTTCTGCATCTTTCGCAATGGTGATTGCCGCAACGGGGCCAAACATTTCTTCCCGAAACGCGGTCATTTCTGGGGTAACATTCGCCAGAGCCGTTGGCGGATAGTAGTTACCTGCCCCAGCCATCtttt                                                                                                 >  2:1254574/1‑140 (MQ=255)                                     ttCCAGTGCATGTTCTGCATCTTTCGCAATGGTGATTGCCGCAACGGGGCCAAACATTTCTTCCCGAAACGCGGTCATTTCTGGGGTAACATTCGCCAGAGCCGTTGGCGGATAGTAGTTACCTGCCCCAGCCATCtttt                                                                                                 >  2:708202/1‑140 (MQ=255)                                          gTGCATGTTCTGCATCTTTCGCAATGGTGATTGCCGCAACGGGGCCAAACATTTCTTCCCGAAACGCGGTCATTTCTGGGGTAACATTCGCCAGAGCCGTTGGCGGATAGTAGTTACCTGCCCCAGCCATCTTTTCCCCg                                                                                            <  1:998687/140‑1 (MQ=255)                                             cATGTTCTGCATCTTTCGCAATGGTGATTGCCGCAACGGGGCCAAACATTTCTTCCCGAAACGCGGTCCTTTCTGGGGTAACATTCGCCAGAGCCGTTGGCGGATAGTAGTTACCTGCCCCAGCCATCTTTTCCCCGccc                                                                                         <  2:974386/140‑1 (MQ=255)                                               tGTTCTGCATCTTTCGCAATGGTGATTGCCGCAACGGGGCCAAACATTTCTTCCCGAAACGCGGTCATTTCTGGGGTAACATTCGCCAGAGCCGTTGGCGGATAGTAGTTACCTGCCCCAGCCATCTTTTCCCCGCCCAg                                                                                       >  2:396705/1‑140 (MQ=255)                                                gTTCTGCATCTTTCGCAATGGTGATTGCCGCAACGGGGCCAAACATTTCTTCCCGAAACGCGGTCATTTCTGGGGTAACATTCGCCAGAGCCGTTGGCGGATAGTAGTTACCTGCCCCAGCCATCTTTTCCCCGCCCAGt                                                                                      >  1:244168/1‑140 (MQ=255)                                                  tCTGCATCTTTCGCAATGGTGATTGCCGCAACGGGGCCAAACATTTCTTCCCGAAACGCGGTCATTTCTGGGGTAACATTCGCCAGAGCCGTTGGCGGATAGTAGTTACCTGCCCCAGCCATCTTTTCCCCGCCCAGTaa                                                                                    <  1:396705/140‑1 (MQ=255)                                                  tCTGCATCTTTCGCAATGGTGATTGCCGCAACGGGGCCAAACATTTCTTCCCGAAACGCGGTCATTTCTGGGGTAACATTCGCCAGAGCCGTTGGCGGATAGTAGTTACCTGCCCCAGCCATCTTTTCCCCGCCCAGTaa                                                                                    <  2:995650/140‑1 (MQ=255)                                                   cTGCATCTTTCGCAATGGTGATTGCCGCAACGGGGCCAAACATTTCTTCCCGAAACGCGGTCATTTCTGGGGTAACATTCGCCAGAGCCGTTGGCGGATAGTAGTTACCTGCCCCAGCCATCTTTTCCCCGCCCAGTAAc                                                                                   >  2:77114/1‑140 (MQ=255)                                                     gCATCTTTCGCAATGGTGATTGCCGCAACGGGGCCAAACATTTCTTCCCGAAACGCGGTCATTTCTGGGGTAACATTCGCCAGAGCCGTTGGCGGATAGTAGTTACCTGCCCCAGCCATCTTTTCCCCGc                                                                                           <  2:167795/130‑1 (MQ=255)                                                     gCATCTTTCGCAATGGTGATTGCCGCAACGGGGCCAAACATTTCTTCCCGAAACGCGGTCATTTCTGGGGTAACATTCGCCAGAGCCGTTGGCGGATAGTAGTTACCTGCCCCAGCCATCTTTTCCCCGc                                                                                           >  1:167795/1‑130 (MQ=255)                                                     gCATCTTTCGCAATGGTGATTGCCGCAACGGGGCCAAACATTTCTTCCCGAAACGCGGTCATTTCTGGGGTAACATTCGCCAGAGCCGTTGGCGGATAGTAGTTACCTGCCCCAGCCATCTTTTCCCCGCCCAGTAACaa                                                                                 >  1:1315611/1‑140 (MQ=255)                                                       aTCTTTCGCAATGGTGATTGCCGCAACGGGGCCAAACATTTCTTCCCGAAACGCGGTCATTTCTGGGGTAACATTCGCCAGAGCCGTTGGCGGATAGTAGTTACCTGCCCCAGCCATCTTTTCCCCGCCCAGTAACAAAc                                                                               >  1:173144/1‑140 (MQ=255)                                                         cTTTCGCAATGGTGATTGCCGCAACGGGGCCAAACATTTCTTCCCGAAACGCGGTCATTTCTGGGGTAACATTCGCCAGAGCCGTTGGCGGATAGTAGTTACCTGCCCCAGCCATCTTTTCCCCGCCCAGTAACAAAcgc                                                                             >  2:575640/1‑140 (MQ=255)                                                          tttCGCAATGGTGATTGCCGCAACGGGGCCAAACATTTCTTCACGAAACGCGGTCATTTCTGGGGTAACATTCGCCAGAGCCGTTGGCGGATAGTAGTTACCTGCCCCAGCCATCTTTTCCCCGCCCAGTAACAAAcgcg                                                                            <  2:1291123/140‑1 (MQ=255)                                                           ttCGCAATGGTGATTGCCGCAACGGGGCCAAACATTTCTTCCCGAAACGCGGTCATTTCTGGGGTAACATTCGCCAGAGCCGTTGGCGGATAGTAGTTACCTGCCCCAGCCATCTTTTCCCCGCCCAGTAACAAACgcgc                                                                           >  2:537378/1‑140 (MQ=255)                                                             cGCAATGGTGATTTCCGCAACGGGGCCAAACATTTCTTCCCGAAACGCGGTCATTTCTGGGGTAACATTCGCCAGAGCCGTTGGCGGATAGTAGTTACCTGCCCCAGCCATCTTTTCCCCGCCCAGTAACAAACGCGCAc                                                                         >  1:1280592/1‑140 (MQ=255)                                                              gCAATGGTGATTGCCGCAACGGGGCCAAACATTTCTTCCCGAAACGCGGTCATTTCTGGGGTAACATTCGCCAGAGCCGTTGGCGGATAGTAGTTACCTGCCCCAGCCATCTTTTCCCCGCCCAGTAACAAACGCGCAcc                                                                        <  2:1280592/140‑1 (MQ=255)                                                                  tGGTGATTGCCGCAACGGGGCCAAACATTTCTTCCCGAAACGCGGTCATTTCTGGGGTAACATTCGCCAGAGCCGTTGGCGGATAGTAGTTACCTGCCCCAGCCATCTTTTCCCCGCCCAGTAACAAACGCGCACCCTgc                                                                    <  2:173144/140‑1 (MQ=255)                                                                     tGATTGCCGCAACGGGGCCAAACATTTCTTCCCGAAACGCGGTCATTTCTGGGGTAACATTCGCCAGAGCCGTTGGCGGATAGTAGTTACCTGCCCCAGCCATCTTTTCCCCGCCCAGTAACAAACGCGCACCCTGCGcc                                                                 <  1:708202/140‑1 (MQ=255)                                                                       aTTGCCGCAACGGGGCCAAACATTTCTTCCCGAAACGCGGTCATTTCTGGGGTAACATTCGCCAGAGCCGTTGGCGGATAGTAGTTACCTGCCCCAGCCATCTTTTCCCCGCCCAGTAACAAACGCGCACCCTGCGCCAg                                                               >  2:212261/1‑140 (MQ=255)                                                                          gCCGCAACGGGGCCAAACATTTCTTCCCGAAACGCGGTCATTTCTGGGGTAACATTCGCCAGAGCCGTTGGCGGATAGTAGTTACCTGCCCCAGCCATCTTTTCCCCGCCCAGTAACAAACGCGCACCCTGCGCCAGGGt                                                            <  2:1247571/140‑1 (MQ=255)                                                                            cGCAACGGGGCCAAACATTTCTTCCCGAAACGCGGTCATTTCTGGGGTAACATTCGCCAGAGCCGTTGGCGGATAGTAGTTACCTGCCCCAGCCATCTTTTCCCCGCCCAGTAACAAACGCGCACCCTGCGCCAGGGttt                                                          >  1:22869/1‑140 (MQ=255)                                                                              cAACGGGGCCAAACATTTCTTCCCGAAACGCGGTCATTTCTGGGGTAACATTCGCCAGAGCCGTTGGCGGATAGTAGTTACCTGCCCCAGCCATCTTTTCCCCGCCCAGTAACAAACGCGCAcc                                                                        <  1:359965/124‑1 (MQ=255)                                                                              cAACGGGGCCAAACATTTCTTCCCGAAACGCGGTCATTTCTGGGGTAACATTCGCCAGAGCCGTTGGCGGATAGTAGTTACCTGCCCCAGCCATCTTTTCCCCGCCCAGTAACAAACGCGCAcc                                                                        >  2:359965/1‑124 (MQ=255)                                                                              cAACGGGGCCAAACATTTCTTCCCGAAACGCGGTCATTTCTGGGGTAACATTCGCCAGAGCCGTTGGCGGATAGTAGTTACCTGCCCCAGCCATCTTTTCCCCGCCCAGTAACAAACGCGCACCCTGCGCCAGGGtttt                                                         >  1:366364/1‑139 (MQ=255)                                                                              cAACGGGGCCAAACATTTCTTCCCGAAACGCGGTCATTTCTGGGGTAACATTCGCCAGAGCCGTTGGCGGATAGTAGTTACCTGCCCCAGCCATCTTTTCCCCGCCCAGTAACAAACGCGCACCCTGCGCCAGGGtttt                                                         <  2:366364/139‑1 (MQ=255)                                                                              cAACGGGGCCAAACATTTCTTCCCGAAACGCGGTCATTTCTGGGGTAACATTCGCCAGAGCCGTTGGCGGATAGTAGTTACCTGCCCCAGCCATCTTTTCCCCGCCCAGTAACAAACGCGCACCCTGCGCCAGGGTTTtc                                                        >  2:798567/1‑140 (MQ=255)                                                                              cAACGGGGCCAAACATTTCTTCCCGAAACGCGGTCATTTCTGGGGTAACATTCGCCAGAGCCGTTGGCGGATAGTAGTTACCTGCCCCAGCCATCTTTTCCCCGCCCAGTAACAAACGCGCACCCTGCGCCAGGGTTTtc                                                        >  1:1029030/1‑140 (MQ=255)                                                                              cAACGGGGCCAAACATTTCTTCCCGAAACGCGGTCATTTCTGGGGTAACATTCGCCAGAGCCGTTGGCGGATAGTAGTTACCTGCCCCAGCCATCTTTTCCCCGCCCAGTAACAAACGCGCACCCTGCGCCAGGGTTTtc                                                        >  2:1395860/1‑140 (MQ=255)                                                                              cAACGGGGCCAAACATTTCTTCCCGAAACGCGGTCATTTCTGGGGTAACATTCGCCAGAGCCGTTGGCGGATAGTAGTTACCTGCCCCAGCCATCTTTTCCCCGCCCAGTAACAAACGCGCACCCTGCGCCAGGGTTTtc                                                        <  1:1395860/140‑1 (MQ=255)                                                                                aCGGGGCCAAACATTTCTTCCCGAAACGCGGTCATTTCTGGGGTAACATTCGCCAGAGCCGTTGGCGGATAGTAGTTACCTGCCCCAGCCATCTTTTCCCCGCCCAGTAACAAACGCGCACCCTGCGCCAGGGTTTtctc                                                      <  1:419964/140‑1 (MQ=255)                                                                                 cGGGGCCAAACATTTCTTCCCGAAACGCGGTCATTTCTGGGGTAACATTCGCCAGAGCCGTTGGCGGATAGTAGTTACCTGCCCCAGCCATCTTTTCCCCGCCCAGTAACAAACGCGCACCCTGCGCCAGGGTTTTCTcc                                                     >  2:1303555/1‑140 (MQ=255)                                                                                  ggggCCAAACATTTCTTCCCGAAACGCGGTCATTTCTGGGGTAACATTCGCCAGAGCCGTTGGCGGATAGTAGTTACCTGCCCCAGCCATCTTTTCCCCGCCCAGTAACAAACGCGCACCCTGCGCCAGGGTTTTCTCCa                                                    <  2:1315611/140‑1 (MQ=255)                                                                                     gCCAAACATTTCTTCCCGAAACGCGGTCATTTCTGGGGTAACATTCGCCAGAGCCGTTGGCGGATAGTAGTTACCTGCCCCAGCCATCTTTTCCCCGCCCAGTAACAAACGCGCACCCTGCGCCAGGGTTTTCTCCACCt                                                 <  1:212261/140‑1 (MQ=255)                                                                                       cAAACATTTCTTCCCGAAACGCGGTCATTTCTGGGGTAACATTCGCCAGAGCCGTTGGCGGATAGTAGTTACCTGCCCCAGCCATCTTTTCCCCGCCCAGTAACAAACGCGCACCCTGCGCCAGGGTTTTCTCCACCtga                                               <  1:968091/140‑1 (MQ=255)                                                                                               tcttcCCGAAACGCGGTCATTTCTGGGGTAACATTCGCCAGAGCCGTTGGCGGATAGTAGTTACCTGCCCCAGCCATCTTTTCCCCGCCCAGTAACAAACGCGCACCCTGCGCCAGGGTTTTCTCCACCTGATGATGCAg                                       <  2:144781/140‑1 (MQ=255)                                                                                                       aaaCGCGGTCATTTCTGGGGTAACATTCGCCAGAGCCGTTGGCGGATAGTAGTTACCTGCCCCAGCCATCTTTTCCCCGCCCAGTAACAAACGCGCACCCTGCGCCAGGGTTTTCTCCACCTGATGATGCAGCTCATCAc                               <  1:798567/140‑1 (MQ=255)                                                                                                                      tGGGGTAACATTCGCCAGAGCCGTTGGCGGATAGTAGTTACCTGCCCCAGCCATCTTTTCCCCGCCCAGTAACAAACGCGCACCCTGCGCCAGGGTTTTCTCCACCTGATGATGCAGCTCATCACGTAAATCAAAACGAg                <  2:1029030/140‑1 (MQ=255)                                                                                                                        gggTAACATTCGCCAGAGCCGTTGGCGGATAGTAGTTACCTGCCCCAGCCATCTTTTCCCCGCCCAGTAACAAACGCGCACCCTGCGCCAGGGTTTTCTCCACCTGATGATGCAGCTCATCACGTAAATCAAAACGAGcc              <  1:575640/140‑1 (MQ=255)                                                                                                                         ggTAACATTCGCCAGAGCCGTTGGCGGATAGTAGTTACCTGCCCCAGCCATCTTTTCCCCGCCCAGTAACAAACGCGCACCCTGCGCCAGGGTTTTCTCCACCTGATGATGCAGCTCATCACGTAAATCAAAACGAGCCa             <  1:537378/140‑1 (MQ=255)                                                                                                                           tAACATTCGCCAGAGCCGTTGGCGGATAGTAGTTACCTGCCCCAGCCATCTTTTCCCCGCCCAGTAACAAACGCGCACCCTGCGCCAGGGTTTTCTCCACCTGATGATGCAGCTCATCACGTAAATCAAAACGAGCCAtt           >  1:225076/1‑140 (MQ=255)                                                                                                                                 tCGCCAGAGCCGTTGGCGGATAGTAGTTACCTGCCCCAGCCATCTTTTCCCCGCCCAGTAACAAACGCGCACCCTGCGCCAGGGTTTTCTCCACCTGATGATGCAGCTCATCACGTAAATCAAAACGAGCCATTGGTCCg     <  2:244168/140‑1 (MQ=255)                                                                                                                                    ccAGAGCCGTTGGCGGATAGTAGTTACCTGCCCCAGCCATCTTTTCCCCGCCCAGTAACAAACGCGCACCCTGCGCCAGGGTTTTCTCCACCTGATGATGCAGCTCATCACGTAAATCAAAACGAGCCATTGGTCCgaga  <  2:225076/140‑1 (MQ=255)                                                                                                                                    ccAGAGCCGTTGGCGGATAGTAGTTACCTGCCCCAGCCATCTTTTCCCCGCCCAGTAACAAACGCGCACCCTGCGCCAGGGTTTTCTCCACCTGATGATGCAGCTCATCACGTAAATCAAAACGAGCCATTGGTCCgaga  >  2:604274/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       GGTCGCTGAAAGGCCGAACTCACTATCATTAGCCAGTTCCAGTGCATGTTCTGCATCTTTCGCAATGGTGATTGCCGCAACGGGGCCAAACATTTCTTCCCGAAACGCGGTCATTTCTGGGGTAACATTCGCCAGAACCGTTGGCGGATAGTAGTTACCTGCCCCAGCCATCTTTTCCCCGCCCAGTAACAAACGCGCACCCTGCGCCAGGGTTTTCTCCACCTGATGATGCAGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGA  >  NC_000913/1613516‑1613786

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑