Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,801,600	T→C	N324S (AAC→AGC)	thrS ←	threonine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,801,600	0	T	C	100.0%	213.1 / NA	58	N324S (AAC→AGC)	thrS	threonine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/39);  total (19/39)

GAACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGGTTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATCTTTGTAGTTGTCCCAGTGACCGGTTTTTTCCCACAGGACACGGTCCATCATGAACGGACCTTTAACTTCCTGATACTGGTACTCTTTCAGTTTAGAACGAACA  >  NC_000913/1801465‑1801731                                                                                                                                        |                                                                                                                                    gAACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGGTTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGCtctc                                                                                                                                 <  1:220136/140‑1 (MQ=255)    ccTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGGTTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGCTCTCaga                                                                                                                              <  2:898324/140‑1 (MQ=255)      tGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGGTTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGCTCTCagaag                                                                                                                            <  1:304889/140‑1 (MQ=255)        aCGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGGTTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGCTCTCAGAAGAt                                                                                                                          <  1:121546/140‑1 (MQ=255)        aCGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGGTTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGCTCTCAGAAGAt                                                                                                                          <  1:135992/140‑1 (MQ=255)             tCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGGTTGAAAATTTGTTCGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGCTCTCAGAAGATgtggt                                                                                                                     <  1:949855/140‑1 (MQ=255)             tCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGGTTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGCTCTCAGAAGATgtggt                                                                                                                     >  2:403732/1‑140 (MQ=255)             tCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGGTTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGCTCTCAGAAGATgtggt                                                                                                                     <  1:1016292/140‑1 (MQ=255)              cGTTACGGTGACAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGGTTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGCTCTCAGAAGATgtggtg                                                                                                                    <  2:1389137/140‑1 (MQ=255)                  aCGGAGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGGTTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGCTCTCAGAAGATGTGGTGAACa                                                                                                                >  2:834524/1‑140 (MQ=255)                   cGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGGTTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGCTCTCAGAAGATGTGGTGAACAt                                                                                                               <  1:836790/140‑1 (MQ=255)                     gttgCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGGTTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGCTCTCAGAAGATGTGGTGAACATTg                                                                                                             <  2:1009118/137‑1 (MQ=255)                     gTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGGTTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGCTCTCAGAAGATGTGGTGAACATTg                                                                                                             <  1:1026005/140‑1 (MQ=255)                     gTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGGTTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGCTCTCAGAAGATGTGGTGAACATTg                                                                                                             <  2:892017/140‑1 (MQ=255)                      tGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGGTTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGCTCTCAGAAGATGTGGTGAACATTGc                                                                                                            <  1:1265312/140‑1 (MQ=255)                        gCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGGTTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGCTCTCAGAAGATGTGGTGAACATTGCAt                                                                                                          <  2:1187438/140‑1 (MQ=255)                          aGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGGTTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGCTCTCAGAAGATGTGGTGAACATTGCATCt                                                                                                        <  1:834524/140‑1 (MQ=255)                                cAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGGTTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGCTCTCAGAAGATGTGGTGAACATTGCATCTTTGTAg                                                                                                  <  2:1016840/140‑1 (MQ=255)                                cAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGGTTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGCTCTCAGAAGATGTGGTGAACATTGCATCTTTGTAg                                                                                                  <  1:1234172/140‑1 (MQ=255)                                      cGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGGTTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGCTCTCAGAAGATGTGGTGAACATTGCATCTTTGTAGTTGTcc                                                                                            <  1:1322082/140‑1 (MQ=255)                                        gCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGGTTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGCTCTCAGAAGATGTGGTGAACATTGCATCTTTGTAGTTGTCCCa                                                                                          <  1:765418/140‑1 (MQ=255)                                             aCGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGGTTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGCTCTCAGAAGATGTGGTGAACATTGCATCTTTGTAGTTGTCCCAGTGAc                                                                                     >  2:1149394/1‑140 (MQ=255)                                                          tCGCGATAAGACTTCAGCCCCTGGTTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGCTCTCAGAAGATGTGGTGAACATTGCATCTTTGTAGTTGTCCCAGTGACCGGTTTTTTCCca                                                                        <  1:544939/140‑1 (MQ=255)                                                          tCGCGATAAGACTTCAGCCCCTGGTTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGCTCTCAGAAGATGTGGTGAACATTGCATCTTTGTAGTTGTCCCAGTGACCGGTTTTTTCCca                                                                        <  2:228718/140‑1 (MQ=255)                                                            gcgATAAGACTTCAGCCCCTGGTTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGCTCTCAGAAGATGTGGTGAACATTGCATCTTTGTAGTTGTCCCAGTGACCGGTTTTTTCCcaca                                                                      <  1:72655/140‑1 (MQ=255)                                                             cgATAAGACTTCAGCCCCTGGTTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGCTCTCAGAAGATGTGGTGAACATTGCATCTTTGTAGTTGTCCCAGTGACCGGTTTTTTCCCACAg                                                                     >  1:971569/1‑140 (MQ=255)                                                               aTAAGACTTCAGCCCCTGGTTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGCTCTCAGAAGATGTGGTGAACATTGCATCTTTGTAGTTGTCCCAGTGACCGGTTTTTTCCCACAGGa                                                                   <  1:1185097/140‑1 (MQ=255)                                                                 aaGACTTCAGCCCCTGGTTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGCTCTCAGAAGATGTGGTGAACATTGCATCTTTGTAGTTGTCCCAGTGACCGGTTTTTTCCCACAGGaca                                                                 <  1:731422/140‑1 (MQ=255)                                                                 aaGACTTCAGCCCCTGGTTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGCTCTCAGAAGATGTGGTGAACATTGCATCTTTGTAGTTGTCCCAGTGACCGGTTTTTTCCCACAGGaca                                                                 <  1:496856/140‑1 (MQ=255)                                                                  aGACTTCAGCCCCTGGTTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGCTCTCAGAAGATGTGGTGAACATTGCATCTTTGTAGTTGTCCCAGTGACCGGTTTTTTCCCACAGGacac                                                                <  1:788779/140‑1 (MQ=255)                                                                     cTTCAGCCCCTGGTTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGCTCTCAGAAGATGTGGTGAACATTGCATCTTTGTAGTTGTCCCAGTGACCGGTTTTTTCCCACAGGac                                                                  <  1:13191/135‑1 (MQ=255)                                                                     cTTCAGCCCCTGGTTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGCTCTCAGAAGATGTGGTGAACATTGCATCTTTGTAGTTGTCCCAGTGACCGGTTTTTTCCCACAGGac                                                                  >  2:13191/1‑135 (MQ=255)                                                                        cAGCCCCTGGTTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGCTCTCAGAAGATGTGGTGAACATTGCATCTTTGTAGTTGTCCCAGTGACCGGTTTTTTCCCACAGGac                                                                  <  1:876041/132‑1 (MQ=255)                                                                        cAGCCCCTGGTTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGCTCTCAGAAGATGTGGTGAACATTGCATCTTTGTAGTTGTCCCAGTGACCGGTTTTTTCCCACAGGac                                                                  >  2:876041/1‑132 (MQ=255)                                                                        cAGCCCCTGGTTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGCTCGCAGAAGATGTGGTGAACATTGCATCTTTGTAGTTGTCCCAGTGACCGGTTTTTTCCCACAGGACACGGTCca                                                          >  2:1270638/1‑140 (MQ=255)                                                                              cTGGTTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGCTCTCAGAAGATGTGGTGAACATTGCATCTTTGTAGTTGTCCCAGTGACCGGTTTTTTCCCACAGGACACGGt                                                             >  2:950768/1‑131 (MQ=255)                                                                              cTGGTTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGCTCTCAGAAGATGTGGTGAACATTGCATCTTTGTAGTTGTCCCAGTGACCGGTTTTTTCCCACAGGACACGGt                                                             <  1:950768/131‑1 (MQ=255)                                                                               tGGTTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGCTCTCAGAAGATGTGGTGAACATTGCATCTTTGTAGTTGTCCCAGTGACCGGTTTTTTCCCACAGGACACGGTCCATCATGaa                                                   <  1:522100/140‑1 (MQ=255)                                                                                ggTTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGCTCTCAGAAGATGTGGTGAACATTGCATCTTTGTAGTTGTCCCAGTGACCGGTTTTTTCCCACAGGACACGGTCCATCATGAAc                                                  <  2:938556/140‑1 (MQ=255)                                                                                   tGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGCTCTCAGAAGATGTGGTGAACATTGCATCTTTGTAGTTGTCCCAGTGACCGGTTTTTTCCCACAGGACACGGTCCATCATGAACGGa                                               <  1:1149394/140‑1 (MQ=255)                                                                                     aaaaTTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGCTCTCAGAAGATGTGGTGAACATTGCATCTTTGTAGTTGTCCCAGTGACCGGTTTTTTCCCACAGGACACGGTCCATCATGAACGGAcc                                             >  2:211576/1‑140 (MQ=255)                                                                                                gTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGCTCTCAGAAGATGTGGTGAACATTGCATCTTTGTAGTTGTCCCAGTGACCGGTTTTTTCCCACAGGACACGGTCCATCATGAACGGACCTTTAACTTCCt                                  <  2:179404/140‑1 (MQ=255)                                                                                                  gACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGCTCTCAGAAGATGTGGTGAACATTGCATCTTTGTAGTTGTCCCAGTGACCGGTTTTTTCCCACAGGACACGGTCCATCATGAACGGACCTTTAACTTCCTGa                                >  1:1306766/1‑140 (MQ=255)                                                                                                  gACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGCTCTCAGAAGATGTGGTGAACATTGCATCTTTGTAGTTGTCCCAGTGACCGGTTTTTTCCCACAGGACACGGTCCATCATGAACGGACCTTTAACTTCCTGa                                >  1:747367/1‑140 (MQ=255)                                                                                                        ggCAGTTCATCGGCTTAATGCAGTATTCACGGCTCTCAGAAGATGTGGTGAACATTGCATCTTTGTAGTTGTCCCAGTGACCGGTTTTTTCCCACAGGACACGGTCCATCATGAACGGACCTTTAACTTCCTGATACTgg                          <  1:403732/140‑1 (MQ=255)                                                                                                        ggCAGTTCATCGGCTTAATGCAGTATTCACGGCTCTCAGAAGATGTGGTGAACATTGCATCTTTGTAGTTGTCCCAGTGACCGGTTTTTTCCCACAGGACACGGTCCATCATGAACGGACCTTTAACTTCCTGATACTgg                          >  2:1244871/1‑140 (MQ=255)                                                                                                          cAGTTCATCGGCTTAATGCAGTATTCACGGCTCTCAGAAGATGTGGTGAACATTGCATCTTTGTAGTTGTCCCAGTGACCGGTTTTTTCCCACAGGACACGGTCCATCATGAACGGACCTTTAACTTCCTGATACTGGTa                        <  2:386793/140‑1 (MQ=255)                                                                                                            gTTCATCGGCTTAATGCAGTATTCACGGCTCTCAGAAGATGTGGTGAACATTGCATCTTTGTAGTTGTCCCAGTGACCGGTTTTTTCCCACAGGACACGGTCCATCATGAACGGACCTTTAACTTCCTGATACTGGTAct                      >  1:1093350/1‑140 (MQ=255)                                                                                                               cATCGGTTTAATGCAGTATTCACGGCTCTCAGAAGATGTGGTGAACATTGCATCTTTGTAGTTGTCCCAGTGACCGGTTTTTTCCCACAGGACACGGTCCATCATGAACGGACCTTTAACTTCCTGATACTGGTACTCtt                   >  1:348614/1‑140 (MQ=255)                                                                                                               cATCGGCTTAATGCAGTATTCACGGCTCTCAGAAGATGTGGTGAACATTGCATCTTTGTAGTTGTCCCAGTGACCGGTTTTTTCCCACAGGACACGGTCCATCATGAACGGACCTTTAACTTCCTGATACTGGTACTCtt                   >  2:1268901/1‑140 (MQ=255)                                                                                                                  cGGCTTAATGCAGTATTCACGGCTCTCAGAAGATGTGGTGAACATTGCATCTTTGTAGTTGTCCCAGTGACCGGTTTTTTCCCACAGGACACGGTCCATCATGAACGGACCTTTAACTTCCTGATACTGGTACTCTTTCa                >  1:859616/1‑140 (MQ=255)                                                                                                                    gCTTAATGCAGTATTCACGGCTCTCAGAAGATGTGGTGAACATTGCATCTTTGTAGTTGTCCCAGTGACCGGTTTTTTCCCACAGGACACGGTCCATCATGAACGGACCTTTAACTTCCTGATACTGGTACTCTTTCAGt              <  1:512024/140‑1 (MQ=255)                                                                                                                     cTTAATGCAGTATTCACGGCTCTCAGAAGATGTGGTGAACATTGCATCTTTGTAGTTGTCCCAGTGACCGGTTTTTTCCCACAGGACACGGTCCATCATGAACGGACCTTTAACTTCCTGATACTGGTAc                       <  2:1190157/130‑1 (MQ=255)                                                                                                                     cTTAATGCAGTATTCACGGCTCTCAGAAGATGTGGTGAACATTGCATCTTTGTAGTTGTCCCAGTGACCGGTTTTTTCCCACAGGACACGGTCCATCATGAACGGACCTTTAACTTCCTGATACTGGTAc                       >  1:1190157/1‑130 (MQ=255)                                                                                                                       tAATGCAGTATTCACGGCTCTCAGAAGATGTGGTGAACATTGCATCTTTGTAGTTGTCCCAGTGACCGGTTTTTTCCCACAGGACACGGTCCATCATGAACGGACCTTTAACTTCCTGATACTGGt                         >  2:489149/1‑126 (MQ=255)                                                                                                                       tAATGCAGTATTCACGGCTCTCAGAAGATGTGGTGAACATTGCATCTTTGTAGTTGTCCCAGTGACCGGTTTTTTCCCACAGGACACGGTCCATCATGAACGGACCTTTAACTTCCTGATACTGGt                         <  1:489149/126‑1 (MQ=255)                                                                                                                                    aCGGCTCTCAGAAGATGTGGTGAACATTGCATCTTTGTAGTTGTCCCAGTGACAGGTTTTTTCCCACAGGACACGGTCCATCATGAACGGACCTTTAACTTCCTGATACTGGTACTCTTTCAGTTTAGAACGaaca  >  1:992842/1‑136 (MQ=255)                                                                                                                                    aCGGCTCTCAGAAGATGTGGTGAACATTGCATCTTTGTAGTTGTCCCAGTGACAGGTTTTTTCCCACAGGACACGGTCCATCATGAACGGACCTTTAACTTCCTGATACTGGTACTCTTTCAGTTTAGAACGaaca  <  2:992842/136‑1 (MQ=255)                                                                                                                                        |                                                                                                                                    GAACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGGTTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATCTTTGTAGTTGTCCCAGTGACCGGTTTTTTCCCACAGGACACGGTCCATCATGAACGGACCTTTAACTTCCTGATACTGGTACTCTTTCAGTTTAGAACGAACA  >  NC_000913/1801465‑1801731

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑