Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,936,458	T→C	intergenic (‑144/‑194)	zwf ← / → yebK	NADP(+)‑dependent glucose‑6‑phosphate dehydrogenase/DNA‑binding transcriptional repressor YebK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,936,458	0	T	C	100.0%	349.6 / NA	95	intergenic (‑144/‑194)	zwf/yebK	NADP(+)‑dependent glucose‑6‑phosphate dehydrogenase/DNA‑binding transcriptional repressor YebK
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (50/45);  total (50/45)

TCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGTGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGACACTGATCATGTTATGAAAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGCACACAGGCGTAATATCTGA  >  NC_000913/1936322‑1936588                                                                                                                                         |                                                                                                                                   tcCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGcgcg                                                                                                                                 >  2:993131/1‑140 (MQ=255) tcCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGcgcg                                                                                                                                 <  2:1364907/140‑1 (MQ=255) tcCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGcgcg                                                                                                                                 >  2:380233/1‑140 (MQ=255)   cTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGCGCGGc                                                                                                                               >  2:1288241/1‑140 (MQ=255)     tAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGCGCGGCaa                                                                                                                             >  2:658934/1‑140 (MQ=255)      aaGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCGCTGTACTGCTTGTACGAGCTTGCGAAAACTGTAAACGCTTATCCGCCCGTGCGATTACGGGAAAAGCGCGCAAAGCGCGGCaaa                                                                                                                            <  1:584448/140‑1 (MQ=255)          taactaacCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGCGCGGCAAAACTg                                                                                                                        >  2:81356/1‑140 (MQ=255)           aactaacCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGCGCGGCAAAACTGa                                                                                                                       >  2:567288/1‑140 (MQ=255)             ctaacCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGCGCGGCAAAACTGATa                                                                                                                     >  2:832172/1‑140 (MQ=255)                acCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGCGCGGCAAAACTGATaaaa                                                                                                                  >  1:1375102/1‑140 (MQ=255)                    ggTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGCGCGGCAAAACTGATAAAAAAat                                                                                                              <  1:81356/140‑1 (MQ=255)                      tACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGCGCGGCAAAACTGATAAAAAAatca                                                                                                            <  2:1375102/140‑1 (MQ=255)                        cTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGCGCGGCAAAACTGATaaaaaaa                                                                                                               <  1:1022846/135‑1 (MQ=255)                        cTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGCGCGGCAAAACTGATaaaaaaa                                                                                                               >  2:1022846/1‑135 (MQ=255)                          tAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGTGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGCGCGGCAAAACTGATAAAAAAATCATCGt                                                                                                        >  1:751273/1‑140 (MQ=255)                          tAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGCGCGGCAAAACTGATAAAAAAATCATCGt                                                                                                        <  1:1288241/140‑1 (MQ=255)                                aGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAATTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGCGCGGCAAAACTGATAAAAAAATCATCGTTTTCCa                                                                                                  <  2:70186/140‑1 (MQ=255)                                aGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGCGCGGCAAAACTGATaa                                                                                                                    >  1:397724/1‑122 (MQ=255)                                aGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGCGCGGCAAAACTGATaa                                                                                                                    <  2:397724/122‑1 (MQ=255)                                aGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGCGCGGCAAAACTGATAAAAAAATCATCGTTTTCCa                                                                                                  >  2:1309782/1‑140 (MQ=255)                                aGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGCGCGGCAAAACTGATAAAAAAATCATCGTTTTCCa                                                                                                  >  2:339348/1‑140 (MQ=255)                                  ggTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGCGCGGCAAAACTGATAAAAAAATCATCGTTTTCCa                                                                                                  <  2:1276250/138‑1 (MQ=255)                                  ggTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGCGCGGCAAAACTGATAAAAAAATCATCGTTTTCCa                                                                                                  >  1:1276250/1‑138 (MQ=255)                                  ggTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGCGCGGCAAAACTGATAAAAAAATCATCGTTTTCCAtt                                                                                                >  2:733720/1‑140 (MQ=255)                                  ggTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGCGCGGCAAAACTGATAAAAAAATCATCGTTTTCCAtt                                                                                                >  2:943975/1‑140 (MQ=255)                                   gTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGCGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTg                                                                                               <  2:1212669/140‑1 (MQ=255)                                   gTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGCGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTg                                                                                               >  1:361011/1‑140 (MQ=255)                                       aCTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGCGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTa                                                                                           >  1:1335758/1‑140 (MQ=255)                                        cTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGCGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTaa                                                                                          <  2:1344024/140‑1 (MQ=255)                                        cTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGCGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTaa                                                                                          <  1:1309782/140‑1 (MQ=255)                                           gTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGCGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTaaaaa                                                                                       >  2:1252563/1‑140 (MQ=255)                                            tAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGCGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAAt                                                                                      >  2:91332/1‑140 (MQ=255)                                            tAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGCGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAAt                                                                                      >  1:256229/1‑140 (MQ=255)                                             aaTTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGCGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATc                                                                                     <  2:1335758/140‑1 (MQ=255)                                                tttCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGCGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGa                                                                                  >  1:441543/1‑140 (MQ=255)                                                tttCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGCGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGa                                                                                  <  2:27655/140‑1 (MQ=255)                                                tttCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGCGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGa                                                                                  <  1:380233/140‑1 (MQ=255)                                                 ttCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGCGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGac                                                                                 <  1:174527/140‑1 (MQ=255)                                                  tCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGCGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAGTCTGaca                                                                                <  2:1198944/140‑1 (MQ=255)                                                  tCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGCGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGaca                                                                                <  1:776005/140‑1 (MQ=255)                                                  tCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGCGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGaca                                                                                <  1:1252563/140‑1 (MQ=255)                                                    ttACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGCGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGACACt                                                                              <  2:256229/140‑1 (MQ=255)                                                      aCGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGCGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGACACTGa                                                                            >  1:830183/1‑140 (MQ=255)                                                          tGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGCGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGaca                                                                                >  2:307102/1‑132 (MQ=255)                                                          tGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGCGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGaca                                                                                <  1:307102/132‑1 (MQ=255)                                                            cACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGCGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGa                                                                                  <  1:1048657/128‑1 (MQ=255)                                                            cACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGCGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGa                                                                                  >  2:1048657/1‑128 (MQ=255)                                                                   cTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGCGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGACACTGATCATGTTATGaaa                                                               >  2:1190656/1‑140 (MQ=255)                                                                    tGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGCGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGACACTGATCATGTTATGaaaa                                                              >  1:386823/1‑140 (MQ=255)                                                                    tGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGCGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGACACTGATCATGTTATGaaaa                                                              >  2:1180039/1‑140 (MQ=255)                                                                    tGCTTTTACGAGCTTGCGAAAACTGTAAACGATTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGCGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGACACTGATCATGTTATGaaaa                                                              >  1:1029484/1‑140 (MQ=255)                                                                      cTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGCGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGACACTGATCATGTTATGaaaaaa                                                            >  1:591303/1‑140 (MQ=255)                                                                         ttACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGCGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGACACTGATCATGTTATGAAAAAAAAt                                                         <  1:943975/140‑1 (MQ=255)                                                                           aCGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGCGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGACACTGATCATGTTATGAAAAAAAATaa                                                       >  2:226067/1‑140 (MQ=255)                                                                           aCGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGCGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGACACTGATCATGTTATGAAAAAAAATaa                                                       >  1:1293456/1‑140 (MQ=255)                                                                           aCGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGCGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGACACTGATCATGTTATGAAAAAAAATaa                                                       >  2:1307246/1‑140 (MQ=255)                                                                              aGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGCGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGACACTGATCATGTTATGAAAAAAAATaacaa                                                    >  1:328888/1‑140 (MQ=255)                                                                              aGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGCGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGACACTGATCATGTTATGAAAAAAAATaacaa                                                    >  1:839205/1‑140 (MQ=255)                                                                               gCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGCGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGACACTGATCATGTTATGAAAAAAAATaacaac                                                   <  1:1307246/140‑1 (MQ=255)                                                                                cTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGCGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGACACTGATCATGTTATGAAAAAAAATAACAACt                                                  >  2:156921/1‑140 (MQ=255)                                                                                cTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGCGAGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGACACTGATCATGTTATGAAAAAAAATAACAACt                                                  >  1:1340976/1‑140 (MQ=255)                                                                                  tGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGCGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGACACTGATCATGTTATGAAAAAAAATAACAACttt                                                >  1:1032306/1‑140 (MQ=255)                                                                                      aaaaCTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGCGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGACACTGATCATGTTATGAAAAAAAATAACAACTTTTTTa                                            <  2:1029484/140‑1 (MQ=255)                                                                                       aaaCTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGCGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGACACTGATCATGTTATGAAAAAAAATAACAACTTTTTTAt                                           <  2:1336345/140‑1 (MQ=255)                                                                                             tAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGCGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGACACTGATCATGTTATGAAAAAAAATAACAACTTTTTTATCTGCtt                                     <  2:839205/140‑1 (MQ=255)                                                                                             tAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGCGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGACACTGATCATGTTATGAAAAAAAATAACAACTTTTTTATCTGCtt                                     <  1:894867/140‑1 (MQ=255)                                                                                             tAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGCGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGACACTGATCATGTTATGAAAAAAAATAACAACTTTTTTATCTGCtt                                     >  1:972883/1‑140 (MQ=255)                                                                                             tAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGCGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGACACTGATCATGTTATGAAAAAAAATAACAACTTTTTTATCTGCtt                                     <  2:328888/140‑1 (MQ=255)                                                                                               aaCGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGCGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGACACTGATCATGTTATGAAAAAAAATAACAACTTTTTTATCTGCtttt                                   <  2:1293456/140‑1 (MQ=255)                                                                                                    ttATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGCGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGACACTGATCATGTTATGAAAAAAAATAACAACTTTTTTATCTGCTTTTGTCAt                              <  2:906374/140‑1 (MQ=255)                                                                                                    ttATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGCGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGACACTGATCATGTTATGAAAAAAAATAACAACTTTTTTATCTGCTTTTGTCAt                              >  1:560713/1‑140 (MQ=255)                                                                                                     tATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGCGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGACACTGATCATGTTATGAAAAAAAATAACAACTTTTTTATCTGCTTTTGTCAtt                             >  2:890626/1‑140 (MQ=255)                                                                                                     tATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGCGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGACACTGATCATGTTATGAAAAAAAATAACAACTTTTTTATCTGCTTTTGTCAtt                             >  1:757071/1‑140 (MQ=255)                                                                                                      aTCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGCGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGACACTGATCATGTTATGAAAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTa                            <  2:441543/140‑1 (MQ=255)                                                                                                      aTCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGCGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGACACTGATCATGTTATGAAAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTa                            <  1:832172/140‑1 (MQ=255)                                                                                                       tCCACCCGTGCGATTGCGGGAAAAGCGCGCAAAGCGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGACACTGATCATGTTATGAAAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTaa                           <  2:1119641/140‑1 (MQ=255)                                                                                                       tCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGCGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGACACTGATCATGTTATGAAAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTaa                           <  2:386823/140‑1 (MQ=255)                                                                                                         cACCCGTGCGATTACGGGAAAAGCGCGCAAAGCGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGACACTGATCATGTTATGAAAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAaca                         <  2:560713/140‑1 (MQ=255)                                                                                                            ccGTGCGATTACGGGAAAAGCGCGCAAAGCGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGACACTGATCATGTTATGAAAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAaca                         >  2:1143383/1‑137 (MQ=255)                                                                                                            ccGTGCGATTACGGGAAAAGCGCGCAAAGCGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGACACTGATCATGTTATGAAAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAaca                         <  1:1143383/137‑1 (MQ=255)                                                                                                            ccGTGCGATTACGGGAAAAGCGCGCAAAGCGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGACACTGATCATGTTATGAAAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACgg                      >  2:730334/1‑140 (MQ=255)                                                                                                              gTGCGATTACGGGAAAAGCGCGCAAAGCGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGACACTGATCATGTTATGAAAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTaa                           >  1:1239539/1‑133 (MQ=255)                                                                                                              gTGCGATTACGGGAAAAGCGCGCAAAGCGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGACACTGATCATGTTATGAAAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTaa                           <  2:1239539/133‑1 (MQ=255)                                                                                                               tGCGATTACGGGAAAAGCGCGCAAAGCGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGACACTGATCATGTTATGAAAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGcac                   >  2:143722/1‑140 (MQ=255)                                                                                                                gCGATTACGGGAAAAGCGCGCAAAGCGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGACACTGATCATGTTATGAAAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGcaca                  <  2:591303/140‑1 (MQ=255)                                                                                                                gCGATTACGGGAAAAGCGCGCAAAGCGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGACACTGATCATGTTATGAAAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGcaca                  <  1:730334/140‑1 (MQ=255)                                                                                                                gCGATTACGGGAAAAGCGCGCAAAGCGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGACACTGATCATGTTATGAAAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGcaca                  <  1:733720/140‑1 (MQ=255)                                                                                                                      aCGGGAAAAGCGCGCAAAGCGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGACACTGATCATGTTATGAAAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGCACACAGGCg            >  1:695914/1‑140 (MQ=255)                                                                                                                      aCGGGAAAAGCGCGCAAAGCGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGACACTGATCATGTTATGAAAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGCACACAGGCg            <  2:751273/140‑1 (MQ=255)                                                                                                                      aCGGGAAAAGCGCGCAAAGCGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGACACTGATCATGTTATGAAAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGCACACAGGCg            <  1:890626/140‑1 (MQ=255)                                                                                                                      aCGGGAAAAGCGCGCAAAGCGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGCAAAAATCTGACACTGATCATGTTATGAAAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGcaca                  <  1:1164460/134‑1 (MQ=255)                                                                                                                      aCGGGAAAAGCGCGCAAAGCGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGCAAAAATCTGACACTGATCATGTTATGAAAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGcaca                  >  2:1164460/1‑134 (MQ=255)                                                                                                                            aaaGCGCGCAAAGCGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGACACTGATCATGTTATGAAAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGCACACAGGCGTAatat      >  1:1031055/1‑140 (MQ=255)                                                                                                                            aaaGCGCGCAAAGCGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGACACTGATCATGTTATGAAAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGCACACAGGCGTAatat      >  2:130005/1‑140 (MQ=255)                                                                                                                              aGCGCGCAAAGCGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGACACTGATCATGTTATGAAAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACgg                      >  2:882049/1‑122 (MQ=255)                                                                                                                              aGCGCGCAAAGCGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGACACTGATCATGTTATGAAAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACgg                      <  1:882049/122‑1 (MQ=255)                                                                                                                              aGCGCGCAAAGCGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGACACTGATCATGTTATGAAAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGCACACAGGCGTAATATCt    <  2:836378/140‑1 (MQ=255)                                                                                                                                cgcgcAAAGCGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGACACTGATCATGTTATGAAAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGCACACAGGCGTAATATCTGa  >  1:696386/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                   TCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGTGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGACACTGATCATGTTATGAAAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGCACACAGGCGTAATATCTGA  >  NC_000913/1936322‑1936588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑