Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,180,736	A→G	S214S (TCA→TCG)	yegU →	putative ADP‑ribosylglycohydrolase YegU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,180,736	0	A	G	100.0%	132.6 / NA	37	S214S (TCA→TCG)	yegU	putative ADP‑ribosylglycohydrolase YegU
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/21);  total (16/21)

GTTGCAGGCGCGGTAGTCATCGCATGGGCGATTTCTCGTGCCATTGACGGAGAAAGCTGGTCAGCGATTGTTGATTCCCTGCCTTCAATTGCGCGACATGCACAGCAAAAACGCATCACTACGTTCAGCGCCTCACTGGCGGCGCGGCTGGAGATTGCGCTGAAAATTGTGCGCAATGCCGACGGCACCGAATCCGCCAGCGAACAGCTTTACCAGGTCGTTGGCGCAGGTACCAGCACTATTGAGTCCGTTCCGTGCGCCATTGC  >  NC_000913/2180602‑2180867                                                                                                                                       |                                                                                                                                    gTTGCAGGCGCGGTAGTCATCGCATGGGCGATTTCTCGTGCCATTGACGGAGAAAGCTGGTCAGCGATTGTTGATTCCCTGCCTTCAATTGCGCGACATGCACAGCAAAAACGCATCACTACGTTCAGCGCCTCGCTggc                                                                                                                                <  2:800339/140‑1 (MQ=255)     cAGGCGCGGTAGTCATCGCATGGGCGATTTCTCGTGCCATTGACGGAGAAAGCTGGTCAGCGATTGTTGATTCCCTGCCTTCAATTGCGCGACATGCACAGCAAAAACGCATCACTACGTTCAGCGCCTCGCTGgcggcg                                                                                                                            <  1:461779/140‑1 (MQ=255)     cAGGCGCGGTAGTCATCGCATGGGCGATTTCTCGTGCCATTGACGGAGAAAGCTGGTCAGCGATTGTTGATTCCCTGCCTTCAATTGCGCGACATGCACAGCAAAAACGCATCACTACGTTCAGCGCCTCGCTGgcggcg                                                                                                                            <  1:779085/140‑1 (MQ=255)     cAGGCGCGGTAGTCATCGCATGGGCGATTTCTCGTGCCATTGACGGAGAAAGCTGGTCAGCGATTGTTGATTCCCTGCCTTCAATTGCGCGACATGCACAGCAAAAACGCATCACTACGTTCAGCGCCTCGCTGgcggcg                                                                                                                            <  2:176633/140‑1 (MQ=255)                   aTCGCATGGGCGATTTCTCGTGCCATTGACGGAGAAAGCTGGTCAGCGATTGTTGATTCCCTGCCTTCAATTGCGCGACATGCACAGCAAAAACGCATCACTACGTTCAGCGCCTCGCTGGCGGCGCGGCTGGAGATTgc                                                                                                              <  2:896051/140‑1 (MQ=255)                        aTGGGCGATTTCTCGTGCCATTGACGGAGAAAGCTGGTCAGCGATTGTTGATTCCCTGCCTTCAATTGCGCGACATGCACAGCAAATACGCATCACTACGTTCAGCGCCTCGCTGGCGGCGCGGCTGGAGATTGCGCTGa                                                                                                         >  2:273401/1‑140 (MQ=255)                        aTGGGCGATTTCTCGTGCCATTGACGGAGAAAGCTGGTCAGCGATTGTTGATTCCCTGCCTTCAATTGCGCGACATGCACAGCAAAAACGCATCACTACGTTCAGCGCCTCGCTGGCGGCGCGGCTGGAGAtt                                                                                                                <  2:565415/133‑1 (MQ=255)                        aTGGGCGATTTCTCGTGCCATTGACGGAGAAAGCTGGTCAGCGATTGTTGATTCCCTGCCTTCAATTGCGCGACATGCACAGCAAAAACGCATCACTACGTTCAGCGCCTCGCTGGCGGCGCGGCTGGAGAtt                                                                                                                >  1:565415/1‑133 (MQ=255)                        aTGGGCGATTTCTCGTGCCATTGACGGAGAAAGCTGGTCAGCGATTGTTGATTCCCTGCCTTCAATTGCGCGACATGCACAGCAAAAACGCATCACTACGTTCAGCGCCTCGCTGGCGGCGCGGCTGGAGATTGCGCTGa                                                                                                         >  2:230293/1‑140 (MQ=255)                             cGATTTCTCGTGCCATTGACGGAGAAAGCTGGTCAGCGATTGTTGATTCCCTGCCTTCAATTGCGCGACATGCACAGCAAAAACGCATCACTACGTTCAGCGCCTCGCTGGCGGCGCGGCTGGAGATTGCGCTGAAAAtt                                                                                                    >  2:913985/1‑140 (MQ=255)                              gATTTCTCGTGCCATTGACGGAGAAAGCTGGTCAGCGATTGTTGATTCCCTGCCTTCAATTGCGCGACATGCACAGCAAAAACGCATCACTACGTTCAGCGCCTCGCTGGCGGCGCGGCTGGAGATTGCGCTGAAAATtg                                                                                                   <  2:916519/140‑1 (MQ=255)                               aTTTCTCGTGCCATTGACGGAGAAAGCTGGTCAGCGATTGTTGATTCCCTGCCTTCAATTGCGCGACATGCACAGCAAAAACGCATCACTACGTTCAGCGCCTCGCTGGCGGCGCGGCTGGAGATTGCGCTGAAAATtgt                                                                                                  >  2:423135/1‑140 (MQ=255)                                           aTTGACGGAGAAAGCTGGTCAGCGATTGTTGATTCCCTGCCTTCAATTGCGCGACATGCACAGCAAAAACGCATCACTACGTTCAGCGCCTCGCTGGCGGCGCGGCTGGAGATTGCGCTGAAAATTGTGCGCAATGCCGa                                                                                      <  2:600242/140‑1 (MQ=255)                                            ttGACGGAGAAAGCTGGTCAGCGATTGTTGATTCCCTGCCTTCAATTGCGCGACATGCACAGCAAAAACGCATCACTACGTTCAGCGCCTCGCTGGCGGCGCGGCTGGAGATTGCGCTGAAAATTGTGCGCAATGCCGAc                                                                                     <  1:1138352/140‑1 (MQ=255)                                              gACGGAGAAAGCTGGTCAGCGATTGTTGATTCCCTGCCTTCAATTGCGCGACATGCACAGCAAAAACGCATCACTACGTTCAGCGCCTCGCTGGCGGCGCGGCTGGAGATTGCGCTGAAAATTGTgcg                                                                                               <  2:670728/128‑1 (MQ=255)                                              gACGGAGAAAGCTGGTCAGCGATTGTTGATTCCCTGCCTTCAATTGCGCGACATGCACAGCAAAAACGCATCACTACGTTCAGCGCCTCGCTGGCGGCGCGGCTGGAGATTGCGCTGAAAATTGTgcg                                                                                               >  1:670728/1‑128 (MQ=255)                                                          tGGTCAGCGATTGTTGATTCCCTGCCTTCAATTGCGCGACATGCACAGCAAAAACGCATCACTACGTTCAGCGCCTCGCTGGCGGCGCGGCTGGAGATTGCGCTGAAAATTGTGCGCAATGCCGACGGCACCGAATCCGc                                                                       <  1:1274657/140‑1 (MQ=255)                                                            gTCAGCGATTGTTGATTCCCTGCCTTCAATTGCGCGACATGCACAGCAAAAACGCATCACTACGTTCAGCGCCTCGCTGGCGGCGCGGCTGGAGATTGCGCTGAAAATTGTGCGCAATGCCGACGGCACCGAATCCGCCa                                                                     >  1:811633/1‑140 (MQ=255)                                                             tCAGCGATTGTTGATTCCCTGCCTTCAATTGCGCGACATGCACAGCAAAAACGCATCACTACGTTCAGCGCCTCGCTGGCGGCGCGGCTGGAGATTGCGCTGAAAATTGTGCGCAATGCCGACGGCACCGAATCCGCCAg                                                                    >  1:18468/1‑140 (MQ=255)                                                                gCGATTGTTGATTCCCTGCCTTCAATTGCGCGACATGCACAGCAAAAACGCATCACTACGTTCAGCGCCTCGCTGGCGGCGCGGCTGGAGATTGCGCTGAAAATTGTGCGCAATGCCGACGGCACCGAATCCGCCAGCGa                                                                 <  2:811633/140‑1 (MQ=255)                                                                gCGATTGTTGATTCCCTGCCTTCAATTGCGCGACATGCACAGCAAAAACGCATCACTACGTTCAGCGCCTCGCTAGCGGCGCGGCTGGAGATTGCGCTGAAAATTGTGCGCAATGCCGACGGAACCGTATCCGCCTGCgg                                                                 <  2:176040/140‑2 (MQ=255)                                                                    ttgttgATTCCCTGCCTTCAATTGCGCGACATGCACAGCAAAAACGCATCACTACGTTCAGCGCCTCGCTGGCGGCGCGGCTGGAGATTGCGCTGAAAATTGTGCGCAATGCCGACGGCACCGAATCCGCCAGCGAACAg                                                             <  1:423135/140‑1 (MQ=255)                                                                      gttgATTCCCTGCCTTCAATTGCGCGACATGCACAGCAAAAACGCATCGCTACGTTCAGTGCCTCGTTGGCGGCGCGCCCGTAGATTTCGCTGAGACTTGTGCGCAAGGCCGATTGCAGCGATCCCGCCAGCGAGCAGCt                                                           <  2:107488/140‑1 (MQ=255)                                                                      gttgATTCCCTGCCTTCAATTGCGCGACATGCACAGCAAAAACGCATCACTACGTTCAGCGCCTCGCTGGCGGCGCGGCTGGAGATTGCGCTGAAAATTGTGCGCAATGCCGACGGCACCGAATCCGCCAGCGAACAGCt                                                           <  1:230293/140‑1 (MQ=255)                                                                              ccTGCCTTCAATTGCGCGACATGCACAGCAAAAACGCATCACTACGTTCAGCGCCTCGCTGGCGGCGCGGCTGGAGATTGCGCTGAAAATTGTGCGCAATGCCGACGGCACCGAATCCGCCAGCGAACAGCTTTACCAgg                                                   >  2:330007/1‑140 (MQ=255)                                                                               cTGCCTTCAATTGCGCGACATGCACAGCAAAAACGCATCACTACGTTCAGCGCCTCGCTGGCGGCGCGGCTGGAGATTGCGCTGAAAATTGTGCGCAATGCCGACGGCACCGAATCCGCCAGCGAACAGCTTTACCAGGt                                                  >  1:405817/1‑140 (MQ=255)                                                                                tGCCTTCAATTGCGCGACATGCACAGCAAAAACGCATCACTACGTTCAGCGCCTCGCTGGCGGCGCGGCTGGAGATTGCGCTGAAAATTGTGCGCAATGCCGACGGCACCGAATCCGCCAGCGAACAGCTTTACCAGGTc                                                 >  2:1287580/1‑140 (MQ=255)                                                                                      cAATTGCGCGACATGCACAACAAAAACGCATCACTACGTTCAGCGCCTCGCTGGCGGCGCGGCTGGAGATTGCGCTGAAAATTGTGCGCAATGCCGACGGCACCGAATCCGCCAGCGAACAGCTTTACCAGGTCGTTGgc                                           >  2:47129/1‑140 (MQ=255)                                                                                       aaTTGCGCGACATGCACAGCAAAAACGCATCACTACGTTCAGCGCCTCGCTGGCGGCGCGGCTGGAGATTGCGCTGAAAATTGTGCGCAATGCCGACGGCACCGAATCCGCCAGCGAACAGCTTTACCAGGTCGTTGgcg                                          <  2:18468/140‑1 (MQ=255)                                                                                       aaTTGCGCGACATGCACAGCAAAAACGCATCACTACGTTCAGCGCCTCGCTGGCGGCGCGGCTGGAGATTGCGCTGAAAATTGTGCGCAATGCCGACGGCACCGAATCCGCCAGCGAACAGCTTTACCAGGTCGTTGgcg                                          >  2:784351/1‑140 (MQ=255)                                                                                         ttGCGCGACATGCACAGCAAAAACGCATCACTACGTTCAGCGCCTCGCTGGCGGCGCGGCTGGAGATTGCGCTGAAAATTGTGCGCAATGCCGACGGCACCGAATCCGCCAGCGAACAGCTTTACCAGGTCGTTGGCGCa                                        >  2:419851/1‑140 (MQ=255)                                                                                                 cATGCACAGCAAAAACGCATCACTACGTTCAGCGCCTCGCTGGCGGCGCGGCTGGAGATTGCGCTGAAAATTGTGCGCAATGCCGACGGCACCGAATCCGCCAGCGAACAGCTTTACCAGGTCGTTGGCGCAGGTACCAg                                <  1:168068/140‑1 (MQ=255)                                                                                                   tGCACAGCAAAAACGCATCACTACGTTCAGCGCCTCGCTGGCGGCGCGGCTGGAGATTGCGCTGAAAATTGTGCGCAATGCCGACGGCACCGAATCCGCCAGCGAACAGCTTTACCAGGTCGTTGGCGCAGGTACCAGCa                              <  1:913985/140‑1 (MQ=255)                                                                                                   tGCACAGCAAAAACGCATCACTACGTTCAGCGCCTCGCTGGCGGCGCGGCTGGAGATTGCGCTGAAAATTGTGCGCAATGCCGACGGCACCGAATCCGCCAGCGAACAGCTTTACCAGGTCGTTGGCGCAGGTACCAGCa                              <  2:1228007/140‑1 (MQ=255)                                                                                                       caGCAAAAACGCATCACTACGTTCAGCGCCTCGCTGGCGGCGCGGCTGGAGATTGCGCTGAAAATTGTGCGCAATGCCGACGGCACCGAATCCGCCAGCGAACAGCTTTACCAGGTCGTTGGCGCAGGTACCAGCACTAt                          <  2:1307775/140‑1 (MQ=255)                                                                                                           aaaaaCGCATCACTACGTTCAGCGCCTCGCTGGCGGCGCGGCTGGAGATTGCGCTGAAAATTGTGCGCAATGCCGACGGCACCGAATCCGCCAGCGAACAGCTTTACCAGGTCGTTGGCGCAGGTACCAGCACTAt                          >  2:1115561/1‑136 (MQ=255)                                                                                                           aaaaaCGCATCACTACGTTCAGCGCCTCGCTGGCGGCGCGGCTGGAGATTGCGCTGAAAATTGTGCGCAATGCCGACGGCACCGAATCCGCCAGCGAACAGCTTTACCAGGTCGTTGGCGCAGGTACCAGCACTAt                          <  1:1115561/136‑1 (MQ=255)                                                                                                                               aGCGCCTCGCTGGCGGCGCGGCTGGAGATTGCGCTGAAAATTGTGCGCAATGCCGACGGCACCGAATCCGCCAGCGAACAGCTTTACCAGGTCGTTGGCGCAGGTACCAGCACTATTGAGTCCGTTCCGTGCGCCATTgc  <  1:660609/140‑1 (MQ=255)                                                                                                                               aGCGCCTCGCTGGCGGCGCGGCTGGAGATTGCGCTGAAAATTGTGCGCAATGCCGACGGCACCGAATCCGCCAGCGAACAGCTTTACCAGGTCGTTGGCGCAGGTACCAGCACTATTGAGTCCGTTCCGTGCGCCATTgc  >  2:1151601/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                    GTTGCAGGCGCGGTAGTCATCGCATGGGCGATTTCTCGTGCCATTGACGGAGAAAGCTGGTCAGCGATTGTTGATTCCCTGCCTTCAATTGCGCGACATGCACAGCAAAAACGCATCACTACGTTCAGCGCCTCACTGGCGGCGCGGCTGGAGATTGCGCTGAAAATTGTGCGCAATGCCGACGGCACCGAATCCGCCAGCGAACAGCTTTACCAGGTCGTTGGCGCAGGTACCAGCACTATTGAGTCCGTTCCGTGCGCCATTGC  >  NC_000913/2180602‑2180867

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑