Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,259,721	A→G	*564Q (TAA→CAA)	fruA ←	fructose‑specific PTS multiphosphoryl transfer protein FruA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,259,721	0	A	G	100.0%	222.1 / NA	65	*564Q (TAA→CAA)	fruA	fructose‑specific PTS multiphosphoryl transfer protein FruA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (31/34);  total (31/34)

ACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAATAGCGCCAGGGATCAGCAGAAC  >  NC_000913/2259590‑2259853                                                                                                                                    |                                                                                                                                     aCGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTGCGCTGCtt                                                                                                                              >  2:12246/1‑140 (MQ=255)    tttGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTGCGCTGCTTTCg                                                                                                                           <  2:662628/140‑1 (MQ=255)    tttGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTGCGCTGCTTTCg                                                                                                                           <  2:444367/140‑1 (MQ=255)        aaGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTGCGCTGCTTTCGCTAc                                                                                                                       <  1:537130/140‑1 (MQ=255)             tCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTGCgct                                                                                                                                  <  2:532350/124‑1 (MQ=255)             tCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTGCgct                                                                                                                                  >  1:532350/1‑124 (MQ=255)             tCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTGCGCTGCTTTCGCTACTGCGt                                                                                                                  >  2:864973/1‑140 (MQ=255)             tCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTGCGCTGCTTTCGCTACTGCGt                                                                                                                  >  2:898544/1‑140 (MQ=255)              cTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTGCGCTGCTTTCGCTACTGCGTc                                                                                                                 <  1:1044468/140‑1 (MQ=255)              cTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTGCGCTGCTTTCGCTACTGCGTc                                                                                                                 <  1:898544/140‑1 (MQ=255)              cTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTGCGCTGCTTTCGCTACTGCGTc                                                                                                                 <  1:705797/140‑1 (MQ=255)                   gAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACGCACCTTTTATTGCGCTGCTTTCGCTACTGCGTCCACtt                                                                                                            <  1:297342/140‑1 (MQ=255)                        aTAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTGCGCTGCTTTCGCTACTGCGTCCACTTCCGGa                                                                                                       <  2:629137/140‑1 (MQ=255)                         tAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTGCGCTGCTTTCGCTACTGCGTCCACTTCCGGa                                                                                                       >  2:44802/1‑139 (MQ=255)                         tAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTGCGCTGCTTTCGCTACTGCGTCCACTTCCGGa                                                                                                       <  1:44802/139‑1 (MQ=255)                                 tttAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTGCGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAgg                                                                                              >  2:749003/1‑140 (MQ=255)                                  ttAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTGCGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGa                                                                                             <  1:1264774/140‑1 (MQ=255)                                  ttAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTGCGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGa                                                                                             >  2:299270/1‑140 (MQ=255)                                  ttAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTGCGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGa                                                                                             >  1:382995/1‑140 (MQ=255)                                       ttCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTGCGCTGCTTTCGCTACTGCGTCCCCTTCCGGACGTTTCAGGAAGGCa                                                                                        <  2:866407/140‑1 (MQ=255)                                       ttCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTGCGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCa                                                                                        <  1:458426/140‑1 (MQ=255)                                         cATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTGCGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATa                                                                                      >  1:552527/1‑140 (MQ=255)                                                ttACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTGCGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCaaa                                                                               <  1:390133/140‑1 (MQ=255)                                                      tCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTGCGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGcc                                                                         <  1:1290065/140‑1 (MQ=255)                                                         caAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTGCGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGccacc                                                                      <  1:579181/140‑1 (MQ=255)                                                                gTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTGCGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAAcc                                                                             >  2:145657/1‑126 (MQ=255)                                                                gTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTGCGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAAcc                                                                             <  1:145657/126‑1 (MQ=255)                                                                  cATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTGCGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTAcc                                                              >  1:614261/1‑139 (MQ=255)                                                                  cATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTGCGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTAcc                                                              <  2:614261/139‑1 (MQ=255)                                                                   aTTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTGCGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCgg                                                            >  2:1378323/1‑140 (MQ=255)                                                                      ctctTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTGCGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCaa                                                         >  1:131446/1‑140 (MQ=255)                                                                       tctTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTGCGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCaat                                                        >  1:1190509/1‑140 (MQ=255)                                                                          ttGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTGCGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCaataat                                                     <  1:1378323/140‑1 (MQ=255)                                                                           tGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTGCGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAAtt                                                    >  2:501105/1‑140 (MQ=255)                                                                              gttcatcaAATGTTACAGGACGGGAAATTTCTGCCCTGTACCACTCCTTTTATCGCGCTGTTTCCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCt                                                 <  2:552527/138‑1 (MQ=255)                                                                              gcTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTGCGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCt                                                 >  2:630693/1‑140 (MQ=255)                                                                               cTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTGCGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTa                                                >  1:499785/1‑140 (MQ=255)                                                                                  atcaAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTGCGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCa                                             >  2:101544/1‑140 (MQ=255)                                                                                   tcaAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTGCGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAg                                            >  2:229536/1‑140 (MQ=255)                                                                                     aaaTGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTGCGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAGACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGt                                          <  2:1274507/140‑1 (MQ=255)                                                                                     aaaTGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTGCGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGt                                          <  2:1184709/140‑1 (MQ=255)                                                                                     aaaTGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTGCGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGt                                          <  1:501105/140‑1 (MQ=255)                                                                                          ttACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTGCGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAAccc                                     >  1:980056/1‑140 (MQ=255)                                                                                            acaggacaggAAATTTCTGCCCTGTAACACACCTTTTATTGCGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCaa                                   >  2:1184163/1‑140 (MQ=255)                                                                                            acaggacaggAAATTTCTGCCCTGTAACACACCTTTTATTGCGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCaa                                   >  2:806737/1‑140 (MQ=255)                                                                                             caggacaggaAATTTCTGCCCTGTAACACACCTTTTATTGCGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAAt                                  <  1:470151/140‑1 (MQ=255)                                                                                              aggacaggaAATTTCTGCCCTGTAACACACCTTTTATTGCGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATa                                 >  2:1216827/1‑140 (MQ=255)                                                                                               ggacaggaAATTTCTGCCCTGTAACACACCTTTTATTGCGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATAc                                <  1:630693/140‑1 (MQ=255)                                                                                                  caggaAATTTCTGCCCTGTAACACACCTTTTATTGCGCTGCATTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCgg                             >  1:1050142/1‑140 (MQ=255)                                                                                                     gaAATTTCTGCCCTGTAACACACCTTTTATTGCGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGt                          >  2:416327/1‑140 (MQ=255)                                                                                                     gaAATTTCTGCCCTGTAACACACCTTTTATTGCGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGt                          >  2:961144/1‑140 (MQ=255)                                                                                                       aaTTTCTGCCCTGTAACACACCTTTTATTGCGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTaa                        <  2:131446/140‑1 (MQ=255)                                                                                                               cccTGTAACACACCTTTTATTGCGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAATAGCGCCa                >  2:321096/1‑140 (MQ=255)                                                                                                               cccTGTAACACACCTTTTATTGCGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAATAGCGCCa                <  2:980056/140‑1 (MQ=255)                                                                                                               cccTGTAACACACCTTTTATTGCGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAATAGCGCCa                <  1:299270/140‑1 (MQ=255)                                                                                                               cccTGTAACACACCTTTTATTGCGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAATAGCGCCa                >  2:207561/1‑140 (MQ=255)                                                                                                                  tGTAACACACCTTTTATTGCGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAATAGCGCCAggg             <  2:1072408/140‑1 (MQ=255)                                                                                                                    tAACACACCTTTTATTGCGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAATAg                     <  1:864323/130‑1 (MQ=255)                                                                                                                    tAACACACCTTTTATTGCGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAATAg                     >  2:864323/1‑130 (MQ=255)                                                                                                                        cccccTTTTATTGCGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCGGAAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAATAGCGCCAGGGATcagc       <  1:1268619/137‑1 (MQ=255)                                                                                                                        acacCTTTTATTGCGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAATAGCGCCAGGGATcagc       <  1:207561/140‑1 (MQ=255)                                                                                                                        acacCTTTTATTGCGCTGCTTTCGCTACTGCGCCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAATAGCGCCAGGGATcagc       <  1:864973/140‑1 (MQ=255)                                                                                                                            cTTTTATTGCGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAATAGCGCCAGGGATCAGCAGaa   >  1:568779/1‑140 (MQ=255)                                                                                                                             ttttATTGCGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAATAGCGCCAGGGATCAGCAGaac  <  1:1184163/140‑1 (MQ=255)                                                                                                                              tttATTGCGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAATAGCGCCAGGGATCAGCAGaac  >  1:1303771/1‑139 (MQ=255)                                                                                                                              tttATTGCGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAATAGCGCCAGGGATCAGCAGaac  <  2:1303771/139‑1 (MQ=255)                                                                                                                                    |                                                                                                                                     ACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAATAGCGCCAGGGATCAGCAGAAC  >  NC_000913/2259590‑2259853

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑