Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,262,445	T→C	T351A (ACC→GCC)	fruB ←	fructose‑specific PTS multiphosphoryl transfer protein FruB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,262,445	0	T	C	100.0%	332.8 / NA	91	T351A (ACC→GCC)	fruB	fructose‑specific PTS multiphosphoryl transfer protein FruB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (45/46);  total (45/46)

CAAGGTCATAAGCCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGTTTGCCGGTACCATCAAGGTTTGTCACGGTAATATCACTGTTAAATTGTTTAATGGTATTGACCA  >  NC_000913/2262317‑2262579                                                                                                                                 |                                                                                                                                       caaGGTCATAAGCCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGCAAAGCGTAGGc                                                                                                                             <  2:767654/140‑1 (MQ=255)    ggTCATAAGCCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGCAAAGCGTAGGCGAt                                                                                                                          >  2:356593/1‑140 (MQ=255)     gTCATAAGCCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGCAAAGCGTAGGCGATg                                                                                                                         >  2:990811/1‑140 (MQ=255)      tCATAAGCCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGCAAAGCGTAGGCGATGa                                                                                                                        >  1:861443/1‑140 (MQ=255)        aTAAGCCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGCAAAGCGTAGGCGATGAcc                                                                                                                      >  2:1268677/1‑140 (MQ=255)               ggATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGCAAAGCGTAGGCGATGACCTTTCTTa                                                                                                               >  1:278603/1‑140 (MQ=255)                gATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGCAAAGCGTAGGCGATGACCTTTCTTaa                                                                                                              >  1:418507/1‑140 (MQ=255)                       ggTGATAGTAGCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGCAAAGCGTAGGCGATGACCTTTCTTAACGCCAAg                                                                                                       >  1:1368610/1‑140 (MQ=255)                          gATAGTAGCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGCAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGc                                                                                                    >  1:1320992/1‑140 (MQ=255)                           aTAGTAGCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGCAAAGCGTAGGCGATGACCTTTCTTAACGcc                                                                                                          <  1:296495/133‑1 (MQ=255)                           aTAGTAGCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGCAAAGCGTAGGCGATGACCTTTCTTAACGcc                                                                                                          >  2:296495/1‑133 (MQ=255)                           aTAGTAGCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGCAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGcc                                                                                                   >  2:186917/1‑140 (MQ=255)                            tagtagCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGCAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCca                                                                                                  <  1:1268677/140‑1 (MQ=255)                             agtagCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGCAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCcac                                                                                                 >  2:286471/1‑140 (MQ=255)                                agCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGCAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAAc                                                                                              >  1:623983/1‑140 (MQ=255)                                 gCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGCAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACt                                                                                             >  1:1202986/1‑140 (MQ=255)                                 gCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGCAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACt                                                                                             >  2:276156/1‑140 (MQ=255)                                  cAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGCAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACtt                                                                                            >  1:853080/1‑140 (MQ=255)                                     cacGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGCAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTtca                                                                                         >  2:366187/1‑140 (MQ=255)                                       cGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGCAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTtcatc                                                                                       >  2:1249764/1‑140 (MQ=255)                                       cGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGCAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTtcatc                                                                                       >  2:100841/1‑140 (MQ=255)                                          cTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGCAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGa                                                                                    >  1:1128965/1‑140 (MQ=255)                                          cTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGCAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGa                                                                                    <  1:286471/140‑1 (MQ=255)                                            gCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGCAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACt                                                                                  >  2:132570/1‑140 (MQ=255)                                                  tATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGCAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTcc                                                                            >  1:66027/1‑140 (MQ=255)                                                  tATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGCAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTcc                                                                            <  1:276156/140‑1 (MQ=255)                                                  tATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGCAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTcc                                                                            >  2:1241895/1‑140 (MQ=255)                                                    tGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGCAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCgt                                                                          >  2:1048845/1‑140 (MQ=255)                                                    tGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGCAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCgt                                                                          >  1:520944/1‑140 (MQ=255)                                                       gcCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGCAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCgtttg                                                                       >  1:590016/1‑140 (MQ=255)                                                       gcCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGCAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCgtttg                                                                       >  1:498627/1‑140 (MQ=255)                                                        cccTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGCAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCgtttgc                                                                      >  2:1166445/1‑140 (MQ=255)                                                          cTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGCAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCgtttgccg                                                                    >  2:188040/1‑140 (MQ=255)                                                            ccccAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGCAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCgtttgccggt                                                                  <  2:853080/140‑1 (MQ=255)                                                            ccccAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGCAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCgtttgccggt                                                                  >  2:766553/1‑140 (MQ=255)                                                             cccAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGCAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCgtttgccggtt                                                                 >  1:351119/1‑140 (MQ=255)                                                                   aCCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGCAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGtttgccggtttgccgg                                                           <  1:366187/140‑1 (MQ=255)                                                                   aCCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGCAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGtttgccggtttgccgg                                                           >  2:1254928/1‑140 (MQ=255)                                                                    ccAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGCAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTttgccggtttgccggt                                                          <  2:1035304/140‑1 (MQ=255)                                                                    ccAGCAGCGACAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGCAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTttgccggtttgccggt                                                          <  2:861443/140‑1 (MQ=255)                                                                     cagcagCGATAGTGTCGCCGATTGCTTTCAGCGCGTGTTCAGCATCTGCTCCCTGGGCGGCAAAGAGTAGGCGAGGTCCTTTCTTCACGCCAAGTGCCCCAACTTCCATCAGACTAAGTCCGTTTGCGGGTTTGAGGGTa                                                         <  2:1313136/140‑1 (MQ=255)                                                                     cagcagCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGCAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGTTTGCCGGTa                                                         <  1:186917/140‑1 (MQ=255)                                                                       gcagcGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGCAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGTTTGCCGGTAcc                                                       >  1:540619/1‑140 (MQ=255)                                                                       gcagcGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGCAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGTTTGCCGGTAcc                                                       >  2:845035/1‑140 (MQ=255)                                                                       gcagcGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGCAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGTTTGCCGGTAcc                                                       >  1:923630/1‑140 (MQ=255)                                                                       gcagcGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGCAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGTTTGCCGGTAcc                                                       >  2:176238/1‑140 (MQ=255)                                                                       gcagcGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGCAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGTTTGCCGGTAcc                                                       >  1:31899/1‑140 (MQ=255)                                                                         agcGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGCAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGTTTGCCGGTACCAt                                                     <  1:199598/140‑1 (MQ=255)                                                                         agcGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGCAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGTTTGCCGGTACCAt                                                     <  2:278603/140‑1 (MQ=255)                                                                           cGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGCAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGTTTGCCGGTACCATCa                                                   <  1:356593/140‑1 (MQ=255)                                                                             aTAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGCAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCgtttgccggt                                                                  <  2:1042049/123‑1 (MQ=255)                                                                             aTAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGCAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCgtttgccggt                                                                  >  1:1042049/1‑123 (MQ=255)                                                                             aTAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGCAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTttgccggtttgccggt                                                          <  2:791016/131‑1 (MQ=255)                                                                             aTAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGCAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTttgccggtttgccggt                                                          >  1:791016/1‑131 (MQ=255)                                                                             aTAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGCAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGTTTGCCGGTACCATCAAg                                                 >  2:364850/1‑140 (MQ=255)                                                                              tAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGCAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGTTTGCCGGTACCATCAAgg                                                <  1:88241/140‑1 (MQ=255)                                                                                  gTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGCAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGTTTGCCGGTACCATCAAGGTTTg                                            >  1:1013992/1‑140 (MQ=255)                                                                                   tCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGCAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGTTTGCCGGTACCATCAAGGTTTGt                                           <  1:990811/140‑1 (MQ=255)                                                                                    cGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGCAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGTTTGCCGGTACCATCAAGGTTTGTc                                          <  2:498627/140‑1 (MQ=255)                                                                                    cGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGCAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGTTTGCCGGTACCATCAAGGTTTGTc                                          <  2:1013992/140‑1 (MQ=255)                                                                                    cGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGCAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGTTTGCCGGTACCATCAAGGTTTGTc                                          <  2:590016/140‑1 (MQ=255)                                                                                    cGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGCAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGTTTGCCGGTACCATCAAGGTTTGTc                                          <  1:1249764/140‑1 (MQ=255)                                                                                     gCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGCAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGTTTGCCGGTACCATCAAGGTTTGTCa                                         <  2:66027/140‑1 (MQ=255)                                                                                      ccGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGCAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGTTTGCCGGTACCATCAAGGTTTGTCAc                                        <  1:100841/140‑1 (MQ=255)                                                                                      ccGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGCAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGTTTGCCGGTACCATCAAGGTTTGTCAc                                        <  1:1254928/140‑1 (MQ=255)                                                                                      ccGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGCAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGTTTGCCGGTACCATCAAGGTTTGTCAc                                        <  1:176238/140‑1 (MQ=255)                                                                                      ccGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGCAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGTTTGCCGGTACCATCAAGGTTTGTCAc                                        <  2:31899/140‑1 (MQ=255)                                                                                      ccGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGCAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGTTTGCCGGTACCATCAAGGTTTGTCAc                                        <  1:641990/140‑1 (MQ=255)                                                                                      ccGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGCAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGTTTGCCGGTACCATCAAGGTTTGTCAc                                        <  1:845035/140‑1 (MQ=255)                                                                                      ccGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGCAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGTTTGCCGGTACCATCAAGGTTTGTCAc                                        <  1:862802/140‑1 (MQ=255)                                                                                          ttGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGCAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGTTTGCCGGTACCATCAAGGTTTGTCAc                                        >  1:792787/1‑136 (MQ=255)                                                                                          ttGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGCAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGTTTGCCGGTACCATCAAGGTTTGTCAc                                        <  2:792787/136‑1 (MQ=255)                                                                                              tttCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGCAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGTTTGCCGGTACCATCAAGGTTTGTCACGGTAatat                                <  1:1241895/140‑1 (MQ=255)                                                                                              tttCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGCAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGTTTGCCGGTACCATCAAGGTTTGTCACGGTAatat                                <  2:623983/140‑1 (MQ=255)                                                                                                tCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGCAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGTTTGCCGGTACCATCAAGGTTTGTCACGGTAATATCa                              <  1:493688/140‑1 (MQ=255)                                                                                                   gcgcCTGTTCAGCATCTGCACCCTGGGCGGCAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGTTTGCCGGTACCATCAAGGTTTGTCACGGTAATATCACTg                           <  1:1168169/140‑1 (MQ=255)                                                                                                      ccTGTTCAGCATCTGCACCCTGGGCGGCAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGTTTGCCGGTACCATCAAGGTTTGTCAc                                        >  1:1102604/1‑124 (MQ=255)                                                                                                      ccTGTTCAGCATCTGCACCCTGGGCGGCAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGTTTGCCGGTACCATCAAGGTTTGTCAc                                        <  2:1102604/124‑1 (MQ=255)                                                                                                         gTTCAGCATCTGCACCCTGGGCGGCAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGTTTGCCGGTACCATCAAGGTTTGTCACGGTAATATCACTGTTAAAt                     >  1:671468/1‑140 (MQ=255)                                                                                                           tCAGCATCTGCACCCTGGGCGGCAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGTTTGCCGGTACCATCAAGGTTTGTCACGGTAATATCACTGTTAAATTg                   <  1:364850/140‑1 (MQ=255)                                                                                                             aGCATCTGCACCCTGGGCGGCAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGTTTGCCGGTACCATCAAGGTTTGTCACGGTAATATCACTGTTAAATTGtt                 <  1:766553/140‑1 (MQ=255)                                                                                                                aTCTGCACCCTGGGCGGCAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGTTTGCCGGTACCATCAAGGTTTGTCACGGTAATATCACTGTTAAATTGTTTaa              <  1:132570/140‑1 (MQ=255)                                                                                                                aTCTGCACCCTGGGCGGCAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGTTTGCCGGTACCATCAAGGTTTGTCACGGTAATATCACTGTTAAATTGTTTaa              <  2:923630/140‑1 (MQ=255)                                                                                                                aTCTGCACCCTGGGCGGCAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGTTTGCCGGTACCATCAAGGTTTGTCACGGTAATATCACTGTTAAATTGTTTaa              <  2:912366/140‑1 (MQ=255)                                                                                                                aTCTGCACCCTGGGCGGCAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGTTTGCCGGTACCATCAAGGTTTGTCACGGTAATATCACTGTTAAATTGTTTaa              <  2:540619/140‑1 (MQ=255)                                                                                                                aTCTGCACCCTGGGCGGCAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGTTTGCCGGTACCATCAAGGTTTGTCACGGTAATATCACTGTTAAATTGTTTaa              <  2:1320992/140‑1 (MQ=255)                                                                                                                        ccTGGGCGGCAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGTTTGCCGGTACCATCAAGGTTTGTCACGGTAATATCACTGTTAAATTGTTTAATGGTATTg      <  2:520944/140‑1 (MQ=255)                                                                                                                         cTGGGCGGCAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGTTTGCCGGTACCATCAAGGTTTGTCACGGTAATATCACTGTTAAATTGTTTAATGGTATTGa     >  1:965694/1‑140 (MQ=255)                                                                                                                          tGGGCGGCAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGTTTGCCGGTACCATCAAGGTTTGTCACGGTAATATCACTGTTAAATTGTTTAATGGTATTGAc    <  1:188040/140‑1 (MQ=255)                                                                                                                          tGGACGGCAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGTCTACGTCCGTTTGCAGGTTTGCCGGTACCATCAAGGATTGTCACGGTAATATCACTGTTAAATTGTTTAATGGTATTGAc    <  2:1128965/140‑1 (MQ=255)                                                                                                                           gggCGGCAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGTTTGCCGGTACCATCAAGGTTTGTCACGGTAATATCACTGTTAAATTGTTTAATGGTATTGAcc   <  1:1048845/140‑1 (MQ=255)                                                                                                                            ggcggcGAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGTTTGCCGGTACCATCAAGGTTTGTCACGGTAATATCACTGTTAAATTGTTTAATGGTATTGAc    <  2:471449/138‑1 (MQ=255)                                                                                                                            ggcggcAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGTTTGCCGGTACCATCAAGGTTTGTCACGGTAATATCACTGTTAAATTGTTTAATGGTATTGAcc   <  1:884808/139‑1 (MQ=255)                                                                                                                            ggcggcAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGTTTGCCGGTACCATCAAGGTTTGTCACGGTAATATCACTGTTAAATTGTTTAATGGTATTGAcc   >  2:884808/1‑139 (MQ=255)                                                                                                                            ggcggcAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGTTTGCCGGTACCATCAAGGTTTGTCACGGTAATATCACTGTTAAATTGTTTAATGGTATTGACCa  >  2:362515/1‑140 (MQ=255)                                                                                                                            ggcggcAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGTTTGCCGGTACCATCAAGGTTTGTCACGGTAATATCACTGTTAAATTGTTTAATGGTATTGACCa  >  2:439084/1‑140 (MQ=255)                                                                                                                             gCGGCAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTCTCATCAGACTACGTCCGTTTGCCGGTTTGCCGGTACCATCAAGGTTTGTCACGGTAATATCACTGTTAAATTGTTTAATGGTa         <  1:1214031/132‑1 (MQ=255)                                                                                                                             gCGGCAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTCTCATCAGACTACGTCCGTTTGCCGGTTTGCCGGTACCATCAAGGTTTGTCACGGTAATATCACTGTTAAATTGTTTAATGGTa         >  2:1214031/1‑132 (MQ=255)                                                                                                                                 |                                                                                                                                       CAAGGTCATAAGCCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACGTCCGTTTGCCGGTTTGCCGGTACCATCAAGGTTTGTCACGGTAATATCACTGTTAAATTGTTTAATGGTATTGACCA  >  NC_000913/2262317‑2262579

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑