Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,317,536	A→C	W781G (TGG→GGG)	rcsC ←	sensory histidine kinase RcsC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,317,536	0	A	C	100.0%	284.6 / NA	78	W781G (TGG→GGG)	rcsC	sensory histidine kinase RcsC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (36/42);  total (36/42)

CATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCACTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTTCGTAT  >  NC_000913/2317402‑2317672                                                                                                                                       |                                                                                                                                         catATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCCCTccc                                                                                                                                     >  1:687864/1‑140 (MQ=255)       gTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCCCTCCCCTGgcg                                                                                                                               >  2:1157868/1‑140 (MQ=255)        tCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCCCTCCCCTGgcgc                                                                                                                              >  1:1058956/1‑140 (MQ=255)        tCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCCCTCCCCTGgcgc                                                                                                                              >  1:664178/1‑140 (MQ=255)             aTCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCCCTCCCCTGGCGCTTtct                                                                                                                         >  2:81533/1‑140 (MQ=255)              tCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCCCTCCCCTGGCGCTTtctc                                                                                                                        <  1:1157868/140‑1 (MQ=255)               cGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCCCTCCCCTGGCGCTTTCTcc                                                                                                                       <  2:1205049/140‑1 (MQ=255)               cGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCCCTCCCCTGGCGCTTTCTcc                                                                                                                       <  1:850815/140‑1 (MQ=255)                 cTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCCCTCCCCTGGCGCTTTCTCCAg                                                                                                                     <  2:264246/140‑1 (MQ=255)                 cTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCCCTCCCCTGGCGCTTTCTCCAg                                                                                                                     >  1:1383196/1‑140 (MQ=255)                 cTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCCCTCCCCTGGCGCTTTCTCCAg                                                                                                                     <  2:424895/140‑1 (MQ=255)                 cTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCCCTCCCCTGGCGCTTTCTCCAg                                                                                                                     <  2:727665/140‑1 (MQ=255)                 cTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCCCTCCCCTGGCGCTTTCTCCAg                                                                                                                     <  1:644791/140‑1 (MQ=255)                   gACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCCCTCCCCTGGCGCTTTCTCCAGCg                                                                                                                   <  1:1263211/140‑1 (MQ=255)                   gACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCCCTCCCCTGGCGCTTTCTCCAGCg                                                                                                                   >  2:647187/1‑140 (MQ=255)                    aCCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCCCTCCCCTGGCGCTTTCTCCAGCgg                                                                                                                  <  2:1109985/140‑1 (MQ=255)                    aCCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCCCTCCCCTGGCGCTTTCTCCAGCgg                                                                                                                  >  1:700698/1‑140 (MQ=255)                      cGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCCCTCCCCTGGCGCTTTCTCCAGCGGaa                                                                                                                <  2:987500/140‑1 (MQ=255)                       gCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCCCTCCCCTGGCGCTTTCTCCAGCGGAAt                                                                                                               <  1:1334355/140‑1 (MQ=255)                                gTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCCCTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATg                                                                                                      >  1:529610/1‑140 (MQ=255)                                gTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCCCTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATg                                                                                                      <  1:1208317/140‑1 (MQ=255)                                  tGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCCCTCCCCTGGCGCTTTCTCCAGCGGAATACGAATATGGc                                                                                                    <  2:1042359/140‑1 (MQ=255)                                  tGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCCCTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGc                                                                                                    >  1:1042359/1‑140 (MQ=255)                                   gACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCCCTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGcg                                                                                                   <  1:5223/140‑1 (MQ=255)                                    aCGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCCCTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGcga                                                                                                  <  2:1267551/140‑1 (MQ=255)                                          gagCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCCCTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAg                                                                                            <  1:1129889/140‑1 (MQ=255)                                          gagCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCCCTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAg                                                                                            <  2:1383196/140‑1 (MQ=255)                                           agCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCCCTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGa                                                                                           >  2:1332940/1‑140 (MQ=255)                                             cGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCCCTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAg                                                                                         >  2:225508/1‑140 (MQ=255)                                               ttAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCCCTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGt                                                                                       <  2:341852/140‑1 (MQ=255)                                               ttAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCCCTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGt                                                                                       <  2:643196/140‑1 (MQ=255)                                                tAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCCCTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGcgacga                                                                                               <  2:165981/131‑1 (MQ=255)                                                tAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCCCTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGcgacga                                                                                               >  1:165981/1‑131 (MQ=255)                                                       aTCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCCCTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCg                                                                               >  2:37745/1‑140 (MQ=255)                                                       aTCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCCCTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCg                                                                               >  2:975889/1‑140 (MQ=255)                                                       aTCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCCCTCCCCTGGCGCTTTCGCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCg                                                                               >  2:1327095/1‑140 (MQ=255)                                                        tcgtcgCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCCCTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGc                                                                              >  1:965628/1‑140 (MQ=255)                                                              ctctCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCCCTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTctgcc                                                                        <  1:125823/140‑1 (MQ=255)                                                                 tcCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCCCTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTctgccctg                                                                     >  2:1088068/1‑140 (MQ=255)                                                                 tcCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCCCTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTctgccctg                                                                     <  2:351038/140‑1 (MQ=255)                                                                   cATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCCCTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTctgccctgcc                                                                   >  1:155082/1‑140 (MQ=255)                                                                   cATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCCCTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTctgccctgcc                                                                   >  2:807559/1‑140 (MQ=255)                                                                     tctcGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCCCTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCAt                                                                 <  1:225508/140‑1 (MQ=255)                                                                      ctcGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCCCTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCAtt                                                                >  2:689090/1‑140 (MQ=255)                                                                        cGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCCCTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCAtttt                                                              <  1:915151/140‑1 (MQ=255)                                                                         gATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCCCTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCAttttt                                                             <  2:51954/140‑1 (MQ=255)                                                                           tCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCCCTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCAttttt                                                             >  1:720785/1‑138 (MQ=255)                                                                           tCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCCCTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCAttttt                                                             <  2:720785/138‑1 (MQ=255)                                                                           tCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCCCTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTa                                                           >  1:267221/1‑140 (MQ=255)                                                                           tCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCCCTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTa                                                           >  2:914784/1‑140 (MQ=255)                                                                             aaataaatACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCCCTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACt                                                         <  2:155082/140‑1 (MQ=255)                                                                             aaataaatACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCCCTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACt                                                         >  1:877574/1‑140 (MQ=255)                                                                              aataaataCGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCCCTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTc                                                        <  2:1296574/140‑1 (MQ=255)                                                                              aataaataCGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCCCTCCCCTGGCGCTTTCTCCAGCCGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTc                                                        <  2:968492/140‑1 (MQ=255)                                                                                taaataCGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCCCTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCAc                                                      <  2:664178/140‑1 (MQ=255)                                                                                       gcgcCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCCCTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCg                                               >  2:7606/1‑140 (MQ=255)                                                                                        cgcCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCCCTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGt                                              <  1:1284158/140‑1 (MQ=255)                                                                                         gcCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCCCTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTc                                             >  2:1230150/1‑140 (MQ=255)                                                                                         gcCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCCCTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTc                                             >  1:828619/1‑140 (MQ=255)                                                                                          ccAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCCCTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCa                                            >  2:119762/1‑140 (MQ=255)                                                                                             acaaTGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCCCTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTg                                         <  1:1230150/140‑1 (MQ=255)                                                                                                aTGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCCCTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATc                                      <  2:529610/140‑1 (MQ=255)                                                                                                 tGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCCCTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCa                                            >  1:369940/1‑133 (MQ=255)                                                                                                 tGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCCCTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCa                                            <  2:369940/133‑1 (MQ=255)                                                                                                     ggTAGCTCATGCGGAGCAGCGACACTGTGTGCCCCCTCCGCTGGGGCTTTGTCCAGCGGAATGCCAATATGGCGACGACAGAAGGTCACTTCCGCTCTGCCCTGCCATTTTTTACTCACTCCCTCGTCAGTGATCAacac                                 <  2:643478/140‑1 (MQ=255)                                                                                                                cGGAGCAGCCACACTGTGTACCCCCTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATc                                      <  2:658972/124‑1 (MQ=255)                                                                                                                cGGAGCAGCCACACTGTGTACCCCCTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATc                                      >  1:658972/1‑124 (MQ=255)                                                                                                                 ggAGCAGCCACACTGTGTACCCCCTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGt                     <  1:535501/140‑1 (MQ=255)                                                                                                                  gAGCAGCCACACTGTGTACCCCCTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTc                    <  1:119762/140‑1 (MQ=255)                                                                                                                       gcCACACTGTGTACCCCCTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTc               <  1:975889/140‑1 (MQ=255)                                                                                                                         cacaCTGTGTACCCCCTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCt             >  2:412034/1‑140 (MQ=255)                                                                                                                           cacTGTGTACCCCCTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGc           <  2:877574/140‑1 (MQ=255)                                                                                                                             cTGTGTACCCCCTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGccc         <  1:7606/140‑1 (MQ=255)                                                                                                                                tgtACCCCCTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGt                     >  2:373674/1‑125 (MQ=255)                                                                                                                                tgtACCCCCTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGt                     <  1:373674/125‑1 (MQ=255)                                                                                                                                  tACCCCCTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCt        <  1:601212/136‑1 (MQ=255)                                                                                                                                  tACCCCCTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCt        >  2:601212/1‑136 (MQ=255)                                                                                                                                  tACCCCCTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTTCGt    >  1:25325/1‑140 (MQ=255)                                                                                                                                  tACCCCCTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTTCGt    >  1:253422/1‑140 (MQ=255)                                                                                                                                  tACCCCCTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTTCGt    <  1:807559/140‑1 (MQ=255)                                                                                                                                   cccccCTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTTCGTa   <  1:360187/139‑1 (MQ=255)                                                                                                                                   aCCCCCTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTTCGTa   <  1:689090/140‑1 (MQ=255)                                                                                                                                    ccccCTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTTCGTAt  <  1:412034/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                         CATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTGTACCCACTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTTCGTAT  >  NC_000913/2317402‑2317672

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑