| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,407,598 | T→C | V13A (GTC→GCC) | alaA → | glutamate‑‑pyruvate aminotransferase AlaA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,407,598 | 0 | T | C | 96.4% | 187.6 / ‑1.4 | 56 | V13A (GTC→GCC) | alaA | glutamate‑‑pyruvate aminotransferase AlaA |
| Reads supporting (aligned to +/- strand): ref base T (1/0); major base C (27/26); minor base A (1/1); total (29/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.21e-01 | |||||||||||
ATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCACGGCAGGGAGTGGCGATAACAGCAAAAAAGGTCAAGATTCATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGTCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAAGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCG > NC_000913/2407462‑2407726 | aTTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCACGGCAGGGAGTGGCGATAACAGCAAAAAAGGTCAAGATTCATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGCCTg < 1:798840/140‑1 (MQ=255)aTTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCACGGCAGGGAGTGGCGATAACAGCAAAAAAGGTCAAGATTCATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGCCTg > 2:882717/1‑140 (MQ=255) aGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCACGGCAGGGAGTGGCGATAACAGCAAAAAAGGTCAAGATTCATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGCCTGTTATGAc < 1:112967/140‑1 (MQ=255) aaaCACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCACGGCAGGGAGTGGCGATAACAGCAAAAAAGGTCAAGATTCATGTCCCCCATTG‑AAAATCCAGCAAATTAGAGAATGCCTGTTATGACATCCGTGGTc < 2:639198/140‑1 (MQ=255) aaCACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCACGGCAGGGAGTGGCGATAACAGCAAAAAAGGTCAAGATTCATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGCCTGTTATGACATCCGTGGTc < 1:221489/140‑1 (MQ=255) ccGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCACGGCAGGGAGTGGCGATAACAGCAAAAAAGGTCAAGATTCATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGCCTGTTATGACATCCGTGGTc > 1:176103/1‑136 (MQ=255) ccGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCACGGCAGGGAGTGGCGATAACAGCAAAAAAGGTCAAGATTCATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGCCTGTTATGACATCCGTGGTc < 2:176103/136‑1 (MQ=255) ccGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCACGGCAGGGAGTGGCGATAACAGCAAAAAAGGTCAAGATTCATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGCCTGTTATGACATCCGTGGTCCGGt > 2:133000/1‑140 (MQ=255) ccGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCACGGCAGGGAGTGGCGATAACAGCAAAAAAGGTCAAGATTCATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGCCTGTTATGACATCCGTGGTCCGGt > 2:672770/1‑140 (MQ=255) ccGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCACGGCAGGGAGTGGCGATAACAGCAAAAAAGGTCAAGATTCATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGCCTGTTATGACATCCGTGGTCCGGt > 1:20728/1‑140 (MQ=255) cGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCACGGCAGGGAGTGGCGATAACAGCAAAAAAGGTCAAGATTCATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGCCTGTTATGACATCCGTGGTCCGGTg > 2:1080743/1‑140 (MQ=255) tCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCACGGCAGGGAGTGGCGATAACAGCAAAAAAGGTCAAGATTCATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGCCTGTTATGa < 2:325148/122‑1 (MQ=255) tCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCACGGCAGGGAGTGGCGATAACAGCAAAAAAGGTCAAGATTCATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGCCTGTTATGa > 1:325148/1‑122 (MQ=255) tCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCACGGCAGGGAGTGGCGATAACAGCAAAAAAGGTCAAGATTCATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGCCTGTTATGACATCCGTGGTc > 2:788322/1‑133 (MQ=255) tCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCACGGCAGGGAGTGGCGATAACAGCAAAAAAGGTCAAGATTCATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGCCTGTTATGACATCCGTGGTc > 2:117430/1‑133 (MQ=255) tCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCACGGCAGGGAGTGGCGATAACAGCAAAAAAGGTCAAGATTCATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGCCTGTTATGACATCCGTGGTc < 1:117430/133‑1 (MQ=255) tCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCACGGCAGGGAGTGGCGATAACAGCAAAAAAGGTCAAGATTCATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGCCTGTTATGACATCCGTGGTc < 1:788322/133‑1 (MQ=255) tCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCACGGCAGGGAGTGGCGATAACAACAAAAAAGGTCAAGATTCATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGCCTGTTATGACATCCGTGGTCCGGTGCt > 2:959633/1‑140 (MQ=255) tCCTGGGGGACTACATTGTTCTAAGCTGACTTCCACGGCAGGGAGTGGCGATAACAGCAAAAAAGGTCAAGATTCATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGCCTGTTATGACATCCGTGGTCCGGTGCt > 2:990104/1‑140 (MQ=255) tACATTGTTCTAAGCTGACTTCCACGGCAGGGAGTGGCGATAACAGCAAAAAAGGTCAAGATTCATGTCCCCCATTGAAAAATCGAGCAAATTAGAGAATGCCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCaa > 1:1386942/1‑140 (MQ=255) tAAGCTGACTTCCACGGCAGGGAGTGGCGATAACAGCAAAAAAGGTCAAGATTCATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGCCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGga > 1:120017/1‑140 (MQ=255) tAAGCTGACTTCCACGGCAGGGAGTGGCGATAACAGCAAAAAAGGTCAAGATTCATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGCCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGga > 2:1181610/1‑140 (MQ=255) aGCTGACTTCCACGGCAGGGAGTGGCGATAACAGCAAAAAAGGTCAAGATTCATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGCCTGTTATGACA‑CCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGgaaga < 2:822419/140‑1 (MQ=255) cTGACTTCCACGGCAGGGAGTGGCGATAACAGCAAAAAAGGTCAAGATTCATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGCCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGgaagaa < 1:1211594/140‑1 (MQ=255) cTGACTTCCACGGCAGGGAGTGGCGATAACAGCAAAAAAGGTCAAGATTCATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGCCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGgaagaa < 1:1181610/140‑1 (MQ=255) cTGACTTCCACGGCAGGGAGTGGCGATAACAGCAAAAAAGGTCAAGATTCATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGCCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTAgaagaa < 1:716054/140‑1 (MQ=255) tGACTTCCACGGCAGGGAGTGGCGATAACAGCAAAAAAGGTCAAGATTCATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGCCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGgaagaag < 2:1068353/140‑1 (MQ=255) gACTTCCACGGCAGGGAGTGGCGATAACAGCAAAAAAGGTCAAGATTCATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGCCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGgaagaaga < 2:1386942/140‑1 (MQ=255) gACTTCCACGGCAGGGAGTGGCGATAACAGCAAAAAAGGTCAAGATTCATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGCCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGgaagaaga < 2:361161/140‑1 (MQ=255) aCTTCCACGGCAGGGAGTGGCGATAACAGCAAAAAAGGTCAAGATTCATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGTCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGgaagaagaa > 2:1201546/1‑140 (MQ=255) aCTTCCACGGCAGGGAGTGGCGATAACAGCAAAAAAGGTCAAGATTCATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGCCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGgaagaagaa > 1:597309/1‑140 (MQ=255) aCTTCCACGGCAGGGAGTGGCGATAACAGCAAAAAAGGTCAAGATTCATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGCCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGgaagaagaa > 1:1294301/1‑140 (MQ=255) cACGGCAGGGAGTGGCGATAACAGCAAAAAAGGTCAAGATTCATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGCCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAAGGTaa < 2:647667/140‑1 (MQ=255) aCGGCAGGGAGTGGCGATAACAGCAAAAAAGGTCAAGATTCATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGCCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAAGGTAAc < 2:120017/140‑1 (MQ=255) cAGGGAGTGGCGATAACAGCAAAAAAGGTCAAGATTCATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGCCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAAGGTaa > 1:967681/1‑135 (MQ=255) cAGGGAGTGGCGATAACAGCAAAAAAGGTCAAGATTCATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGCCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAAGGTaa < 2:967681/135‑1 (MQ=255) cAGGGAGTGGCGATAACAGCAAAAAAGGTCAAGATTCATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGCCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAAGGTAACAAgg > 1:204850/1‑140 (MQ=255) aaCAGCAAAAAAGGTCAAGATTCATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGCCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAAGGTAACAAGGTACTGAAACTGAAc < 2:943165/140‑1 (MQ=255) aaCAGCAAAAAAGGTCAAGATTCATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGCCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAAGGTAACAAGGTACTGAAACTGAAc < 2:1188088/140‑1 (MQ=255) aaCAGCAAAAAAGGTCAAGATTCATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGACTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAAGGTAACAAGGTACTGAAACTGAAc > 1:1003013/1‑140 (MQ=255) cAAAAAAGGTCAAGATTCATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGCCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAAGGTAACAAGGTACTGAAACTGAACATCgg > 2:743203/1‑140 (MQ=255) cAAAAAAGGTCAAGATTCATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGCCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAAGGTAACAAGGTACTGAAACTGAACATCgg < 2:1302203/140‑1 (MQ=255) cAAAAAAGGTCAAGATTCATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGCCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAAGGTAACAAGGTACTGAAACTGAACATCgg < 1:616859/140‑1 (MQ=255) ccccATTGAAAAATCCAGCAAATTAGAGAATGCCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAAGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTg > 2:583470/1‑140 (MQ=255) ccccATTGAAAAATCCAGCAAATTAGAGAATGCCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAAGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTg > 1:473286/1‑140 (MQ=255) ccccATTGAAAAATCCAGCAAATTAGAGAATGCCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAAGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTg > 2:917734/1‑140 (MQ=255) ccccATTGAAAAATCCAGCAAATTAGAGAATGCCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAAGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTg > 2:971225/1‑140 (MQ=255) ccATTGAAAAATCCAGCAAATTAGAGAATGCCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAAGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGAc < 2:982856/140‑1 (MQ=255) cATTGAAAAATCCAGCAAATTAGAGAATGCCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAAGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAg > 2:653970/1‑122 (MQ=255) cATTGAAAAATCCAGCAAATTAGAGAATGCCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAAGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAg < 1:653970/122‑1 (MQ=255) ttGAAAAATCCAGCAAATTAGAGAATGCCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAAGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGAcgcg > 1:558952/1‑140 (MQ=255) tGAAAAATCCAGCAAATTAGAGAATGCCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAAGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACgcgc < 2:20728/140‑1 (MQ=255) tGAAAAATCCAGCAAATTAGAGAATGACTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAAGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACgcgc < 2:1003013/140‑1 (MQ=255) aaaaaTCCAGCAAATTAGAGAATGCCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAAGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCa > 2:686844/1‑140 (MQ=255) aTCCAGCAAATTAGAGAATGCCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAACAAAACGCCTGGAAGAAAAAGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATg > 1:198499/1‑140 (MQ=255) tCCAGCAAATTAGAGAATGCCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAAGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGa > 2:125433/1‑140 (MQ=255) aTTAGAGAATGCCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAAGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCg < 2:826114/140‑1 (MQ=255) | ATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCACGGCAGGGAGTGGCGATAACAGCAAAAAAGGTCAAGATTCATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGTCTGTTATGACATCCGTGGTCCGGTGCTGAAAGAAGCAAAACGCCTGGAAGAAGAAGGTAACAAGGTACTGAAACTGAACATCGGCAACCCAGCCCCGTTCGGTTTTGACGCGCCAGATGAAATCCTCG > NC_000913/2407462‑2407726 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |