Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,417,658	T→C	V193A (GTC→GCC)	yfcC →	putative transporter YfcC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,417,658	0	T	C	100.0%	254.1 / NA	70	V193A (GTC→GCC)	yfcC	putative transporter YfcC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (43/27);  total (43/27)

TTCTCTTTATTCCTGCGCTGTTTATTCTGTTTTCACTTGGCGGCGCGGTATTTGGTATGGGAGAAGAGGCCGTCGCCTTTGCCATTATCATCGCACCGCTAATGGTCCGGCTGGGCTATGACAGTATTACCACCGTCCTGGTGACCTATATTGCCACGCAAATCGGTTTTGCCAGTTCGTGGATGAACCCGTTTTGTGTGGTCGTTGCTCAGGGGATTGCCGGCGTTCCGGTGCTTTCTGGCTCCGGGTTGCGCATCGTGGTGTGGGT  >  NC_000913/2417523‑2417790                                                                                                                                        |                                                                                                                                     ttCTCTTTATTCCTGCGCTGTTTATTCTGTTTTCACTTGGCGGCGCGGTATTTGGTATGGGAGAAGAGGCCGTCGCCTTTGCCATTATCATCGCACCGCTAATGGTCCGGCTGGGCTATGACAGTATTACCACCGCCCTg                                                                                                                                  <  1:537961/140‑1 (MQ=255) ttCTCTTTATTCCTGCGCTGTTTATTCTGTTTTCACTTGGCGGCGCGGTATTTGGTATGGGAGAAGAGGCCGTCGCCTTTGCCATTATCATCGCACCGCTAATGGTCCGGCTGGGCTATGACAGTATTACCACCGCCCTg                                                                                                                                  <  2:1396080/140‑1 (MQ=255)    tctTTATTCCTGCGCTGTTTATTCTGTTTTCACTTGGCGGCGCGGTATTTGGTATGGGCGAAGAGGCCGTCGCCTTTGCCATTATCATCGCACCGCTAATGGTCCGGCTGGGCTATGACAGTATTACCACCGCCCTGGTg                                                                                                                               <  2:1224287/140‑1 (MQ=255)    tctTTATTCCTGCGCTGTTTATTCTGTTTTCACTTGGCGGCGCGGTATTTGGTATGGGAGAAGAGGCCGTCGCCTTTGCCATTATCATCGCACCGCTAATGGTCCGGCTGGGCTATGACAGTATTACCACCGCCCTGGTg                                                                                                                               <  2:735638/140‑1 (MQ=255)      tttATTCCTGCGCTGTTTATTCTGTTTTCACTTGGCGGCGCGGTATTTGGTATGGGAGAAGAGGCCGTCGCCTTTGCCATTATCATCGCACCGCTAATGGTCCGGCTGGGCTATGACAGTATTACCACCGCCCTGGTGAc                                                                                                                             >  1:792047/1‑140 (MQ=255)        tATTCCTGCGCTGTTTATTCTGTTTTCACTTGGCGGCGCGGTATTTGGTATGGGAGAAGAGGCCGTCGCCTTTGCCATTATCATCGCACCGCTAATGGTCCGGCTGGGCTATGACAGTATTACCACCGCCCTGGCGACCt                                                                                                                           >  2:205180/1‑140 (MQ=255)              tGCGCTGTTTATTCTGTTTTCACTTGGCGGCGCGGTATTTGGTATGGGAGAAGAGGCCGTCGCCTTTGCCATTATCATCGCACCGCTAATGGTCCGGCTGGGCTATGACAGTATTACCACCGCCCTGGTGACCTATATTg                                                                                                                     >  1:848898/1‑140 (MQ=255)               gcgcTGTTTATTCTGTTTTCACTTGGCGGCGCGGTATTTGGTATGGGAGAAGAGGCCGTCGCCTTTGCCATTATCATCGCACCGCTAATGGTCCGGCTGGGCTATGACAGTATGACCACCGCCCTGGTGACCTATATTGc                                                                                                                    >  2:978385/1‑140 (MQ=255)                cgcTGTTTATTCTGTTTTCACTTGGCGGCGCGGTATTTGGTATGGGAGAAGAGGCCGTCGCCTTTGCCATTATCATCGCACCGCTAATGGTCCGGCTGGGCTATGACAGTATTACCACCGCCCTGGTGACCTATATTGcc                                                                                                                   >  2:467244/1‑140 (MQ=255)                cgcTGTTTATTCTGTTTTCACTTGGCGGCGCGGTATTTGGTATGGGAGAAGAGGCCGTCGCCTTTGCCATTATCATCGCACCGCTAATGGTCCGGCTGGGCTATGACAGTATTACCACCGCCCTGGTGACCTATATTGcc                                                                                                                   >  1:388997/1‑140 (MQ=255)                               tttCACTTGGCGGCGCGGTATTTGGTATGGGAGAAGAGGCCGTCGCCTTTGCCATTATCATCGCACCGCTAATGGTCCGGCTGGGCTATGACAGTATTACCACCGCCCTGGTGACCTATATTGCCACGCAAATCGGtttt                                                                                                    >  1:171688/1‑140 (MQ=255)                               tttCACTTGGCGGCGCGGTATTTGGTATGGGAGAAGAGGCCGTCGCCTTTGCCATTATCATCGCACCGCTAATGGTCCGGCTGGGCTATGACAGTATTACCACCGCCCTGGTGACCTATATTGCCACGCAAATCGGtttt                                                                                                    >  1:671382/1‑140 (MQ=255)                                      tGGCGGCGCGGTATTTGGTATGGGAGAAGAGGCCGTCGCCTTTGCCATTATCATCGCACCGCTAATGGTCCGGCTGGGCTATGACAGTATTACCACCGCCCTGGTGACCTATATTGCCACGCAAATCGGTTTTGCCAGtt                                                                                             <  2:171688/140‑1 (MQ=255)                                        gcggcgCGGTATTTGGTATGGGAGAAGAGGCCGTCGCCTTTGCCATTATCATCGCACCGCTAATGGTCCGGCTGGGCTATGACAGTATTACCACCGCCCTGGTGACCTATATTGCCACGCAAATCGGTTTTGCCAGTTCg                                                                                           >  2:1197232/1‑140 (MQ=255)                                            cgcgGTATTTGGTATGGGAGAAGAGGCCGTCGCCTTTGCCATTATCATCGCACCGCTAATGGTCCGGCTGGGCTATGACAGTATTACCACCGCCCTGGTGACCTATATTGCCACGCAAATCGGTTTTGCCAGTTCGTGGa                                                                                       >  2:386696/1‑140 (MQ=255)                                            cgcgGTATTTGGTATGGGAGAAGAGGCCGTCGCCTTTGCCATTATCATCGCACCGCTAATGGTCCGGCTGGGCTATGACAGTATTACCACCGCCCTGGTGACCTATATTGCCACGCAAATCGGTTTTGCCAGTTCGTGGa                                                                                       <  2:885940/140‑1 (MQ=255)                                              cgGTATTTGGTATGGGAGAAGAGGCCGTCGCCTTTGCCATTATCATCGCACCGCTAATGGTCCGGCTGGGCTATGACAGTATTACCACCGCCCTGGTGACCTATATTGCCACGCAAATCGGTTTTGCCAGTTCGTGGATg                                                                                     >  2:1051622/1‑140 (MQ=255)                                               ggTATTTGGTATGGGAGAAGAGGCCGTCGCCTTTGCCATTATCATCGCACCGCTAATGGTCCGGCTGGGCTATGACAGTATTACCACCGCCCTGGTGACCTATATTGCCACGCAAATCGGTTTTGCCAGTTCGTGGATGa                                                                                    >  1:62694/1‑140 (MQ=255)                                                    ttGGTATGGGAGAAGAGGCCGTCGCCTTTGCCATTATCATCGCACCGCTAATGGTCCGGCTGGGCTATGACAGTATTACCACCGCCCTGGTGACCTATATTGCCACGCAAATCGGTTTTGCCAGTTCGTGGATGAACCCg                                                                               <  2:576654/140‑1 (MQ=255)                                                    ttGGTATGGGAGAAGAGGCCGTCGCCTTTGCCATTATCATCGCACCGCTAATGGTCCGGCTGGGCTATGACAGTATTACCACCGCCCTGGTGACCTATATTGCCACGCAAATCGGTTTTGCCAGTTCGTGGATGAACCCg                                                                               <  2:1122675/140‑1 (MQ=255)                                                           gggAGAAGAGGCCGTCGCCTTTGCCATTATCATCGCACCGCTAATGGTCCGGCTGGGCTATGACAGTATTACCACCGCCCTGGTGACCTATATTGCCACGCAAATCGGTTTTGCCAGTTCGTGGATGAACCCGTTTtgtg                                                                        >  2:1164861/1‑140 (MQ=255)                                                             gagaAGAGGCCGTCGCCTTTGCCATTATCATCGCACCGCTAATGGTCCGGCTGGGCTATGACAGTATTACCACCGCCCTGGTGACCTATATTGCCACGCAAATCGGTTTTGCCAGTTCGTGGATGAACCCGTTTtgtgtg                                                                      >  1:552431/1‑140 (MQ=255)                                                             gagaAGAGGCCGTCGCCTTTGCCATTATCATCGCACCGCTAATGGTCCGGCTGGGCTATGACAGTATTACCACCGCCCTGGTGACCTATATTGCCACGCAAATCGGTTTTGCCAGTTCGTGGATGAACCCGTTTtgtgtg                                                                      >  2:1191852/1‑140 (MQ=255)                                                               gaagaGGCCGTCGCCTTTGCCATTATCATCGCACCGCTAATGGTCCGGCTGGGCTATGACAGTATTACCACCGCCCTGGTGACCTATATTGCCACGCAAATCGGTTTTGCCAGTTCGTGGATGAACCCGTTTTGTGTGGt                                                                    <  2:503599/140‑1 (MQ=255)                                                                 agagGCCGTCGCCTTTGCCATTATCATCGCACCGCTAATGGTCCGGCTGGGCTATGACAGTATTACCACCGCCCTGGTGACCTATATTGCCACGCAAATCGGTTTTGCCAGTTCGTGGATGAACCCGTTTTGTGTGGTCg                                                                  <  2:62694/140‑1 (MQ=255)                                                                 agagGCCGTCGCCTTTGCCATTATCATCGCACCGCTAATGGTCCGGCTGGGCTATGACAGTATTACCACCGCCCTGGTGACCTATATTGCCACGCAAATCGGTTTTGCCAGTTCGTGGATGAACCCGTTTTGTGTGGTCg                                                                  <  1:1191852/140‑1 (MQ=255)                                                                 agagGCCGTCGCCTTTGCCATTATCATCGCACCGCTAATGGTCCGGCTGGGCTATGACAGTATTACCACCGCCCTGGTGACCTATATTGCCACGCAAATCGGTTTTGCCAGTTCGTGGATGAACCCGTTTTGTGTGGTCg                                                                  >  1:857202/1‑140 (MQ=255)                                                                     gCCGTCGCCTTTGCCATTATCATCGCACCGCTAATGGTCCGGCTGGGCTATGACAGTATTACCACCGCCCTGGTGACCTATATTGCCACGCAAATCGGTTTTGCCAGTTCGTGGATGAACCCGTTTTGTGTGGTCGTTGc                                                              >  2:366731/1‑140 (MQ=255)                                                                       cGTCGCCTTTGCCATTATCATCGCACCGCTAATGGTCCGGCTGGGCTATGACAGTATTACCACCGCCCTGGTGACCTATATTGCCACGCAAATCGGTTTTGCCAGTTCGTGGATGAACCCGTTTTGTGTGGTCGTTGCTc                                                            <  1:1200017/140‑1 (MQ=255)                                                                        gTCGCCTTTGCCATTATCATCGCACCGCTAATGGTCCGGCTGGGCTATGACAGTATTACCACCGCCCTGGTGACCTATATTGCCACGCAAATCGGTTTTGCCAGTTCGTGGATGAACCCGTTTTGTGTGGTCGTTGCTCa                                                           >  2:1045763/1‑140 (MQ=255)                                                                          cGCCTTTGCCATTATCATCGCACCGCTAATGGTCCGGCTGGGCTATGACAGTATTGCCACCGCCCTGGTGACCTATATTGCCACGCAAATCGGTTTTGCCAGTTCGTGGATGAACCCGTTTTGTGTGGTCGTTGCTCAgg                                                         >  2:538445/1‑140 (MQ=255)                                                                             cTTTGCCATTATCATCGCACCGCTAATGGTCCGGCTGGGCTATGACAGTATTACCACCGCCCTGGTGACCTATATTGCCACGCAAATCGGTTTTGCCAGTTCGTGGATGAACCCGTTTTGTGTGGTCGTTGCTCAGGGGa                                                      >  2:31301/1‑140 (MQ=255)                                                                              tttGCCATTATCATCGCACCGCTAATGGTCCGGCTGGGCTATGACAGTATTACCACCGCCCTGGTGACCTATATTGCCACGCAAATCGGTTTTGCCAGTTCGTGGATGAACCCGTTTTGTGTGGTCGTTGCTCAGGGGAt                                                     <  1:1027461/140‑1 (MQ=255)                                                                               ttGCCATTATCATCGCACCGCTAATGGTCCGGCTGGGCTATGACAGTATTACCACCGCCCTGGTGACCTATATTGCCACGCAAATCGGTTTTGCCAGTTCGTGGATGAACCCGTTTTGTGTGGTCGTTGCTCAGGGGAtt                                                    <  2:792047/140‑1 (MQ=255)                                                                                tGCCATTATCATCGCACCGCTAATGGTCCGGCTGGGCTATGACAGTATTACCACCGCCCTGGTGACCTATATTACCACGCAAATCGGTTTTGCCAGTTCGTGGATGAACCCGTTTTGTGTGGTCGTTGCTCAGGGGATTg                                                   >  2:865242/1‑140 (MQ=255)                                                                                       atcatcGCACCGCTAATGGTCCGGCTGGGCTATGACAGTATTACCACCGCCCTGGTGACCTATATTGCCACGCAAATCGGTTTTGCCAGTTCGTGGATGAACCCGTTTTGTGTGGTCGTTGCTCAGGGGATTGCCGGCGt                                            >  2:840981/1‑140 (MQ=255)                                                                                         catcGCACCGCTAATGGTCCGGCTGGGCTATGACAGTATTACCACCGCCCTGGTGACCTATATTGCCACGCAAATCGGTTTTGCCAGTTCGTGGATGAACCCGTTTTGTGTGGTCGTTGCTCAGGGGATTGCCGGCGTTc                                          <  1:366731/140‑1 (MQ=255)                                                                                         catcGCACCGCTAATGGTCCGGCTGGGCTATGACAGTATTACCACCGCCCTGGTGACCTATATTGCCACGCAAATCGGTTTTGCCAGTTCGTGGATGAACCCGTTTTGTGTGGTCGTTGCTCAGGGGATTGCCGGCGTTc                                          >  1:62766/1‑140 (MQ=255)                                                                                         catcGCACCGCTAATGGTCCGGCTGGGCTATGACAGTATTACCACCGCCCTGGTGACCTATATTGCCACGCAAATCGGTTTTGCCAGTTCGTGGATGAACCCGTTTTGTGTGGTCGTTGCTCAGGGGATTGCCGGCGTTc                                          >  2:411398/1‑140 (MQ=255)                                                                                              cACCGCTAATGGTCCGGCTGGGCTATGACAGTATTACCACCGCCCTGGTGACCTATATTGCCACGCAAATCGGTTTTGCCAGTTCGTGGATGAACCCGTTTTGTGTGGTCGTTGCTCAGGGGATTGCCGGCGTTCCGGTg                                     <  1:1051622/140‑1 (MQ=255)                                                                                               aCCGCTAATGGTCCGGCTGGGCTATGACAGTATTACCACCGCCCTGGTGACCTATATTGCCACGCAAATCGGTTTTGCCAGTTCGTGGATGAACCCGTTTTGTGTGGTCGTTGCTCAGGGGATTGCCGGCGTTCCGGTGc                                    >  1:232760/1‑140 (MQ=255)                                                                                               aCCGCTAATGGTCCGGCTGGGCTATGACAGTATTACCACCGCCCTGGTGACCTATATTGCCACGCAAATCGGTTTTGCCAGTTCGTGGATGAACCCGTTTTGTGTGGTCGTTGCTCAGGGGATTGCCGGCGTTCCGGTGc                                    >  2:928894/1‑140 (MQ=255)                                                                                               aCCGCTAATGGTCCGGCTGGGCTATGACAGTATTACCACCGCCCTGGTGACCTATATTGCCACGCAAATCGGTTTTGCCAGTTCGTGGATGAACCCGTTTTGTGTGGTCGTTGCTCAGGGGATTGCCGGCGTTCCGGTGc                                    >  2:1128016/1‑140 (MQ=255)                                                                                                 cGCTAATGGTCCGGCTGGGCTATGACAGTATTACCACCGCCCTGGTGACCTATATTGCCACGCAAATCGGTTTTGCCAGTTCGTGGATGAACCCGTTTTGTGTGGTCGTTGCTCAGGGGATTGCCGGCGTTCCGGTGCtt                                  >  1:490167/1‑140 (MQ=255)                                                                                                   cTAATGGTCCGGCTGGGCTATGACAGTATTACCACCGCCCTGGTGACCTATATTGCCACGCAAATCGGTTTTGCCAGTTCGTGGATGAACCCGTTTTGTGTGGTCGTTGCTCAGGGGATTGCCGGCGTTCCGGTGCTTTc                                >  1:328748/1‑140 (MQ=255)                                                                                                   cTAATGGTCCGGCTGGGCTATGACAGTATTACCACCGCCCTGGTGACCTATATTGCCACGCAAATCGGTTTTGCCAGTTCGTGGATGAACCCGTTTTGTGTGGTCGTTGCTCAGGGGATTGCCGGCGTTCCGGTGCTTTc                                >  2:641749/1‑140 (MQ=255)                                                                                                    tAATGGTCCGGCTGGGCTATGACAGTATTACCACCGCCCTGGTGACCTATATTGCCACGCAAATCGGTTTTGCCAGTTCGTGGATGAACCCGTTTTGTGTGGTCGTTGCTCAGGGGATTGCCGGCGTTCCGGTGCTTTCt                               >  2:662542/1‑140 (MQ=255)                                                                                                      aTGGTCCGGCTGGGCTATGACAGTATTACCACCGCCCTGGTGACCTATATTGCCACGCAAATCGGTTTTGCCAGTTCGTGGATGAACCCGTTTTGTGTGGTCGTTGCTCAGGGGATTGCCGGCGTTCCGGTGCTTTCTgg                             >  1:701703/1‑140 (MQ=255)                                                                                                         gTCCGGCTGGGCTATGACAGTATTACCACCGCCCTGGTGACCTATATTGCCACGCAAATCGGTTTTGCCAGTTCGTGGATGAACCCGTTTTGTGTGGTCGTTGCTCAGGGGATTGCCGGCGTTCCGGTGCTTTCTGGCTc                          >  1:493/1‑140 (MQ=255)                                                                                                            cGGCTGGGCTATGACAGTATTACCACCGCCCTGGTGACCTATATTGCCACGCAAATCGGTTTTGCCAGTTCGTGGATGAACCCGTTTTGTGTGGTCGTTGCTCAGGGGATTGCCGGCGTTCCGGTGCTTTCTGGCTCCgg                       >  2:605694/1‑140 (MQ=255)                                                                                                            cGGCTGGGCTATGACAGTATTACCACCGCCCTGGTGACCTATATTGCCACGCAAATCGGTTTTGCCAGTTCGTGGATGAACCCGTTTTGTGTGGTCGTTGCTCAGGGGATTGCCGGCGTTCCGGTGCTTTCTGGCTCCgg                       >  2:1258338/1‑140 (MQ=255)                                                                                                             ggCTGGGCTATGACAGTATTACCACCGCCCTGGTGACCTATATTGCCACGCAAATCGGTTTTGCCAGTTCGTGGATGAACCCGTTTTGTGTGGTCGTTGCTCAGGGGATTGCCGGCGTTCCGGTGCTTTCTGGCTCCggg                      <  2:701703/140‑1 (MQ=255)                                                                                                             ggCTGGGCTATGACAGTATTACCACCGCCCTGGTGACCTATATTGCCACGCAAATCGGTTTTGCCAGTTCGTGGATGAACCCGTTTTGTGTGGTCGTTGCTCAGGGGATTGCCGGCGTTCCGGTGCTTTCTGGCTCCggg                      >  1:45819/1‑140 (MQ=255)                                                                                                             ggCTGGGCTATGACAGTATTACCACCGCCCTGGTGACCTATATTGCCACGCAAATCGGTTTTGCCAGTTCGTGGATGAACCCGTTTTGTGTGGTCGTTGCTCAGGGGATTGCCGGCGTTCCGGTGCTTTCTGGCTCCggg                      >  1:1387050/1‑140 (MQ=255)                                                                                                                 gggCTATGACAGTATTACCACCGCCCTGGTGACCTATATTGCCACGCAAATCGGTTTTGCCAGTTCGTGGATGAACCCGTTTTGTGTGGTCGTTGCTCAGGGGATTGCCGGCGTTCCGGTGCTTTCTGGCTCCGGGTTgc                  <  1:605694/140‑1 (MQ=255)                                                                                                                 gggCTATGACAGTATTACCACCGCCCTGGTGACCTATATTGCCACGCAAATCGGTTTTGCCAGTTCGTGGATGAACCCGTTTTGTGTGGTCGTTGCTCAGGGGATTGCCGGCGTTCCGGTGCTTTCTGGCTCCGGGTTgc                  <  1:1197232/140‑1 (MQ=255)                                                                                                                  ggCTATGACAGTATTACCACCGCCCTGGTGACCTATATTGCCACGCAAATCGGTTTTGCCAGTTCGTGGATGAACCCGTTTTGTGTGGTCGTTGCTCAGGGGATTGCCGGCGTTCCGGTGCTTTCTGGCTCCGGGTTgcg                 <  1:681671/140‑1 (MQ=255)                                                                                                                   gCTATGACAGTATTACCACCGCCCTGGTGACCTATATTGCCACGCAAATCGGTTTTGCCAGTTCGTGGATGAACCCGTTTTGTGTGGTCGTTGCTCAGGGGATTGCCGGCGTTCCGGTGCTTTCTGGCTCCGGGTTgcgc                >  1:1277742/1‑140 (MQ=255)                                                                                                                    cTATGACAGTATTACCACCGCCCTGGTGACCTATATTGCCACGCAAATCGGTTTTGCCAGTTCGTGGATGAACCCGTTTTGTGTGGTCGTTGCTCAGGGGATTGCCGGCGTTCCGGTGCTTTCTGGCTCCGGGTTGCGCa               >  1:629750/1‑140 (MQ=255)                                                                                                                    cTATGACAGTATTACCACCGCCCTGGTGACCTATATTGCCACGCAAATCGGTTTTGCCAGTTCGTGGATGAACCCGTTTTGTGTGGTCGTTGCTCAGGGGATTGCCGGCGTTCCGGTGCTTTCTGGCTCCGGGTTGCGCa               <  2:629750/140‑1 (MQ=255)                                                                                                                    cTATGACAGTATTACCACCGCCCTGGTGACCTATATTGCCACGCAAATCGGTTTTGCCAGTTCGTGGATGAACCCGTTTTGTGTGGTCGTTGCTCAGGGGATTGCCGGCGTTCCGGTGCTTTCTGGCTCCGGGTTGCGCa               <  1:1347958/140‑1 (MQ=255)                                                                                                                     tATGACAGTATTACCACCGCCCTGGTGACCTATATTGCCACGCAAATCGGTTTTGCCAGTTCGTGGATGAACCCGTTTTGTGTGGTCGTTGCTCAGGGGATTGCCGGCGTTCCGGTGCTTTCTGGCTCCGGGTTGCGCAt              <  1:641749/140‑1 (MQ=255)                                                                                                                      aTGACAGTATTACCACCGCCCTGGTGACCTATATTGCCACGCAAATCGGTTTTGCCAGTTCGTGGATGAACCCGTTTTGTGTGGTCGTTGCTCAGGGGATTGCCGGCGTTCCGGTGCTTTCTGGCTCCGGGTTGCGCATc             <  2:671382/140‑1 (MQ=255)                                                                                                                       tGACAGTATTGCCACCGCCCTGGTGACCTATATTGCCACGCAAATCGGTTTTGCCAGTTCGTGGATGAACCCGTTTTGTGTGGTCGTTGCTCAGGGGATTGCCGGCGTTCCGGTGCTTTCTGGCTCCGGGTTGCGCATCg            <  1:538445/140‑1 (MQ=255)                                                                                                                             tATTACCACCGCCCTGGTGACCTATATTGCCACGCAAATCGGTTTTGCCAGTTCGTGGATGAACCCGTTTTGTGTGGTCGTTGCTCAGGGGATTGCCGGCGTTCCGGTGCTTTCTGGCTCCGGGTTGCGCATCGTGgtgt      >  1:373262/1‑140 (MQ=255)                                                                                                                              aTTACCACCGCCCTGGTGACCTATATTGCCACGCAAATCGGTTTTGCCAGTTCGTGGATGAACCCGTTTTGTGTGGTCGTTGCTCAGGGGATTGCCGGCGTTCCGGTGCTTTCTGGCTCCGGGTTGCGCATCGTGGtgtg     >  2:185622/1‑140 (MQ=255)                                                                                                                                 accaccGCCCTGGTGACCTATATTGCCACGCAAATCGGTTTTGCCAGTTCGTGGATGAACCCGTTTTGTGTGGTCGTTGCTCAGGGGATTGCCGGCGTTCCGGTGCTTTCTGGCTCCGGGTTGCGCATc             >  1:1166191/1‑129 (MQ=255)                                                                                                                                 accaccGCCCTGGTGACCTATATTGCCACGCAAATCGGTTTTGCCAGTTCGTGGATGAACCCGTTTTGTGTGGTCGTTGCTCAGGGGATTGCCGGCGTTCCGGTGCTTTCTGGCTCCGGGTTGCGCATc             <  2:1166191/129‑1 (MQ=255)                                                                                                                                 accaccGCCCTGGTGACCTATATTGCCACGCAAATCGGTTTTGCCAGTTCGTGGATGAACCCGTTTTGTGTGGTCGTTGCTCAGGGGATTGCCGGCGTTCCGGTGCTTTCTGGCTCCGGGTTGCGCATCGTGGTGTGGGt  <  2:493/140‑1 (MQ=255)                                                                                                                                 accaccGCCCTGGTGACCTATATTGCCACGCAAATCGGTTTTGCCAGTTCGTGGATGAACCCGTTTTGTGTGGTCGTTGCTCAGGGGATTGCCGGCGTTCCGGTGCTTTCTGGCTCCGGGTTGCGCATCGTGGTGTGGGt  >  1:102425/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                     TTCTCTTTATTCCTGCGCTGTTTATTCTGTTTTCACTTGGCGGCGCGGTATTTGGTATGGGAGAAGAGGCCGTCGCCTTTGCCATTATCATCGCACCGCTAATGGTCCGGCTGGGCTATGACAGTATTACCACCGTCCTGGTGACCTATATTGCCACGCAAATCGGTTTTGCCAGTTCGTGGATGAACCCGTTTTGTGTGGTCGTTGCTCAGGGGATTGCCGGCGTTCCGGTGCTTTCTGGCTCCGGGTTGCGCATCGTGGTGTGGGT  >  NC_000913/2417523‑2417790

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑