Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,547,852	T→C	F394L (TTT→CTT)	murP →	N‑acetylmuramic acid‑specific PTS enzyme IICB component/anhydro‑N‑acetylmuramic acid transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,547,852	0	T	C	100.0%	192.9 / NA	55	F394L (TTT→CTT)	murP	N‑acetylmuramic acid‑specific PTS enzyme IICB component/anhydro‑N‑acetylmuramic acid transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/28);  total (27/28)

CGCTGGCACTCTACTGGCGGGCGCAACCGCACAGTGCGCTACGCAGTCAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGTTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGCCTGGTGGGGTCTGCCGATGGGCTTAAACAGCGCCTTTGGGCCGTCTGGTCTGGTAGCGCTGCCGCTGATGACTTCCGC  >  NC_000913/2547721‑2547985                                                                                                                                    |                                                                                                                                      cgcTGGCACTCTACTGGCGGGCGCAACCGCACAGTGCGCTACGCAGTCAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGCTTGTTAcc                                                                                                                               <  2:1379573/140‑1 (MQ=255)  gcTGGCACTCTACTGGCGGGCGCAACCGCACAGTGCGCTACGCAGTCAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGCTTGTTACCg                                                                                                                              <  2:106718/140‑1 (MQ=255)             aCTGGCGGGCGCAACCGCACAGTGCGCTACGCAGTCAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGCTTGTTACCGCCTGTTTAggc                                                                                                                   <  1:125122/140‑1 (MQ=255)                          aCCGCACAGTGCGCTACGCAGTCAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCCCCCTGCCCCGCATGAAGCCGCTTGTTACCGCCTGTTTAGGCGGCGCggcgggcg                                                                                                      <  2:1053508/140‑1 (MQ=255)                                  gTGCGCTACGCAGTCAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGCTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGgtttgttt                                                                                              >  2:1256873/1‑140 (MQ=255)                                     cgcTACGCAGTCAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGCTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTa                                                                                             <  2:1047521/138‑1 (MQ=255)                                     cgcTACGCAGTCAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGCTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTa                                                                                             >  1:1047521/1‑138 (MQ=255)                                     cgcTACGCAGTCAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGCTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATc                                                                                           >  2:653762/1‑140 (MQ=255)                                     cgcTACGCAGTCAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGCTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATc                                                                                           >  1:1233035/1‑140 (MQ=255)                                     cgcTACGCAGTCAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGCTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATc                                                                                           >  1:67528/1‑140 (MQ=255)                                     cgcTACGCAGTCAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGCTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATc                                                                                           >  1:618270/1‑140 (MQ=255)                                     cgcTACGCAGTCAGGTACGCGGGGCGATTATTCCAGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGCTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATc                                                                                           >  1:592909/1‑140 (MQ=255)                                             aGTCAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGCTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGAt                                                                                   >  2:463787/1‑140 (MQ=255)                                                cAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGCTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGc                                                                                <  1:266294/140‑1 (MQ=255)                                                cAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGCTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGc                                                                                <  2:288679/140‑1 (MQ=255)                                                    tACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGCTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGCCtgg                                                                            >  2:950831/1‑140 (MQ=255)                                                    tACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGCTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGCCtgg                                                                            <  2:57538/140‑1 (MQ=255)                                                    tACGCGGGGCGATTATTCCCGGCCTCCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGCTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGCCtgg                                                                            >  2:1219335/1‑140 (MQ=255)                                                     aCGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCCCCCTGCCCCGCATGAAGCCGCTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGCCtggt                                                                           <  1:489452/140‑1 (MQ=255)                                                         ggggCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGCTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGCCTGGTgggg                                                                       >  2:1343020/1‑140 (MQ=255)                                                         ggggCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGCTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGCCTGGTgggg                                                                       >  2:455063/1‑140 (MQ=255)                                                         ggggCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGCTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGCCTGGTgggg                                                                       >  1:158077/1‑140 (MQ=255)                                                         ggggCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGCTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGCTTATCGGCCTGATAGCCTGGTgggg                                                                       >  2:568082/1‑140 (MQ=255)                                                           ggCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGCTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGCCTGGTGGGGTc                                                                     >  2:48255/1‑140 (MQ=255)                                                                 tattCCCGGCCTGCTGGGCGTTGGTGACCCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGCTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGCCTGGTGGGGTCTGCCGa                                                               <  1:974395/140‑1 (MQ=255)                                                                 tattCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGCTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGCCTGGTGGGGTCTGCCGa                                                               <  2:158077/140‑1 (MQ=255)                                                                 tattCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGCTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGCCTGGTGGGGTCTGCCGa                                                               <  2:954655/140‑1 (MQ=255)                                                                 tattCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGCTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGCCTGGTGGGGTCTGCCGa                                                               <  1:950831/140‑1 (MQ=255)                                                                 tattCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGCTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGCCTGGTGGGGTCTGCCGa                                                               <  1:1343020/140‑1 (MQ=255)                                                                 tattCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGCTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGCCTGGTGGGGTCTGCCGa                                                               <  1:455063/140‑1 (MQ=255)                                                                 tattCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGCTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGCCTGGTGGGGTCTGCCGa                                                               <  1:558989/140‑1 (MQ=255)                                                                   ttCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGCTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGCCTGGTGGGGTCTGCCGATg                                                             <  1:653762/140‑1 (MQ=255)                                                                        ggCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGCTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGCCTGGTGGGGTCTGCCGAt                                                              <  2:319750/134‑1 (MQ=255)                                                                        ggCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGCTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGCCTGGTGGGGTCTGCCGAt                                                              >  1:319750/1‑134 (MQ=255)                                                                          ccTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGCTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGCCTGGTGGGGTCTGCCGATGGGCTTaa                                                      >  2:1053649/1‑140 (MQ=255)                                                                              ctgGGCGTTGGTGACCCGCTGATTTACGGTGTCACCCTGCCCGGCATGAAGCCGCTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGCCTGGTGGGGTCTGCCGATGGGCTTAAACAg                                                  <  1:1042288/140‑1 (MQ=255)                                                                                       ggTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGCTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGCCTGGTGGGGTCTGCCGATGGGCTTAAACAGCGCCTTTgg                                         <  1:195445/140‑1 (MQ=255)                                                                                           aaCCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGCTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGCCTGGTGGGGTCTGCCGATGGGCTTAAACAg                                                  <  2:1088794/127‑1 (MQ=255)                                                                                           aaCCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGCTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGCCTGGTGGGGTCTGCCGATGGGCTTAAACAg                                                  >  1:1088794/1‑127 (MQ=255)                                                                                             ccGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGCTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGCCTGGTGGGGTCTGCCGATGGGCTTAAACAGCGCCTTTGGGCCgtc                                   <  2:592909/140‑1 (MQ=255)                                                                                               gCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGCTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGCCTGGTGGGGTCTGCCGATGGGCTTAAACAGCGCCTTTGGGCCgtctg                                 <  1:974120/140‑1 (MQ=255)                                                                                                cTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGCTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGCCTGGTGGGGTCTGCCGATGGGCTTAAACAg                                                  >  2:74882/1‑122 (MQ=255)                                                                                                cTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGCTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGCCTGGTGGGGTCTGCCGATGGGCTTAAACAg                                                  <  1:74882/122‑1 (MQ=255)                                                                                                cTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGCTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGCCTGGTGGGGTCTGCCGATGGGCTTAAACAGCGCCTTTGGGCCgtctgg                                >  2:1386465/1‑140 (MQ=255)                                                                                                        cGGTGTCACCCTGCCCCGCATGAAGCCGCTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGCCTGGTGGGGTCTGCCGATGGGCTTAAACAGCGCCTTTGGGCCgtctgg                                >  2:916765/1‑132 (MQ=255)                                                                                                        cGGTGTCACCCTGCCCCGCATGAAGCCGCTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGCCTGGTGGGGTCTGCCGATGGGCTTAAACAGCGCCTTTGGGCCgtctgg                                <  1:916765/132‑1 (MQ=255)                                                                                                          gtgtCACCCTGCCCCGCATGAAGCCGCTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGCCTGGTGGGGTCTGCCGATGGGCTTAAACAGCGCCTTTGGGCCGTCTGGTCTGGTAgcg                      >  2:1331827/1‑140 (MQ=255)                                                                                                             tCACCCTGCCCCGCATGAAGCCGCTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGCCTGGTGGGGTCTGCCGATGGGCTTAAACAGCGCCTTTGGGCCGTCTGGTCTGGTAGCGCTg                   >  1:637008/1‑140 (MQ=255)                                                                                                              cACCCTGCCCCGCATGAAGCCGCTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGCCTGGTGGGGTCTGCCGATGGGCTTAAACAGCGCCTTTGGGCCGTCTGGTCTGGTAGCGCTGc                  <  1:1216724/140‑1 (MQ=255)                                                                                                                cccTGCCCCGCATGAAGCCGCTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGCCTGGTGGGGTCTGCCGATGGGCTTAAACAGCGCCTTTGGGCCGTCTGGTCTGGTAGCGCTGCCg                <  1:48255/140‑1 (MQ=255)                                                                                                                   tGCCCCGCATGAAGCCGCTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGCCTGGTGGGGTCTGCCGATGGGCTTAAACAGCGCCTTTGGGCCGTCTGGTCTGGTAGCGCTGCCGCtg             >  1:1031087/1‑140 (MQ=255)                                                                                                                     ccccGCATGAAGCCGCTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGCCTGGTGGGGTCTGCCGATGGGCTTAAACAGCGCCTTTGGGCCGTCTGGTCTGGTAGCGGTGCCGCtgaa           >  2:891577/1‑139 (MQ=255)                                                                                                                         gCATGAAGCCGCTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGCCTGGTGGGGTCTGCCGATGGGCTTAAACAGCGCCTTTGGGCCGTCTGGTCTGGTAGCGCTGCCGCTGATGACt       >  1:43237/1‑140 (MQ=255)                                                                                                                          cATGAAGCCGCTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGCCTGGTGGGGTCTGCCGATGGGCTTAAACAGCGCCTTTGGGCCGTCTGGTCTGGTAGCGCTGCCGCTGATGACtt      >  2:361243/1‑140 (MQ=255)                                                                                                                              aaGCCGCTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGCCTGGTGGGGTCTGCCGATGGGCTTAAACAGCGCCTTTGGGCCGTCTGGTCTGGTAGCGCTGCCGCTGATGACTTCCGc  <  1:463787/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                      CGCTGGCACTCTACTGGCGGGCGCAACCGCACAGTGCGCTACGCAGTCAGGTACGCGGGGCGATTATTCCCGGCCTGCTGGGCGTTGGTGAACCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGTTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGCCTGGTGGGGTCTGCCGATGGGCTTAAACAGCGCCTTTGGGCCGTCTGGTCTGGTAGCGCTGCCGCTGATGACTTCCGC  >  NC_000913/2547721‑2547985

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑