Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,565,473	T→C	intergenic (+141/+8)	yffS → / ← eutA	CPZ‑55 prophage; uncharacterized protein YffS/reactivating factor for ethanolamine ammonia‑lyase EutBC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,565,473	0	T	C	100.0%	217.8 / NA	60	intergenic (+141/+8)	yffS/eutA	CPZ‑55 prophage; uncharacterized protein YffS/reactivating factor for ethanolamine ammonia‑lyase EutBC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/31);  total (29/31)

CCTTCAACCCCATCTCATCAATCAACGCCCGGCCCCGCTGCCGGGTTTTTGCTATGCACCACAATTACCCCAACCGGATACACAGCCGGATACAATTCCACCAGCACCCAGCCACCCAGCGCCACCGCTGGCGAATACCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAGTCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTG  >  NC_000913/2565338‑2565609                                                                                                                                        |                                                                                                                                         ccTTCAACCCCATCTCATCAATCAACGCCCGGCCCCGCTGCCGGGTTTTTGCTATGCACCACAATTACCCCAACCGGATACACAGCCGGATACAATTCCACCAGCACCCAGCCACCCAGCGCCACCGCTGGCGAACACCg                                                                                                                                      >  1:1014889/1‑140 (MQ=255)   ttCAACCCCATCTCATCAATCAACGCCCGGCCCCGCTGCCGGGTTTTTGCTATGCACCACAATTACCCCAACCGGATACACAGCCGGATACAATTCCACCAGCACCCAGCCACCCAGCGCCACCGCTGGCGAACACCGCa                                                                                                                                    <  2:594806/140‑1 (MQ=255)     cAACCCCATCTCATCAATCAACGCCCGGCCCCGCTGCCGGGTTTTTGCTATGCACCACAATTACCCCAACCGGATACACAGCCGGATACAATTCCACCAGCACCCAGCCACCCAGCGCCACCGCTGGCGAACACCGCAtt                                                                                                                                  >  1:1017483/1‑140 (MQ=255)     cAACCCCATCTCATCAATCAACGCCCGGCCCCGCTGCCGGGTTTTTGCTATGCACCACAATTACCCCAACCGGATACACAGCCGGATACAATTCCACCAGCACCCAGCCACCCAGCGCCACCGCTGGCGAACACCGCAtt                                                                                                                                  >  1:335332/1‑140 (MQ=255)           cATCTCATCAATCAACGCCCGGCCCCGCTGCCGGGTTTTTGCTATGCACCACAATTACCCCAACCGGATACACAGCCGGATACAATTCCACCAGCACCCAGCCACCCAGCGCCACCGCTGGCGAACACCGCATTCaggaa                                                                                                                            <  1:98215/140‑1 (MQ=255)             tctcATCAATCAACGCCCGGCCCCGCTGCCGGGTTTTTGCTATGCACCACAATTACCCCAACCGGATACACAGCCGGATACAATTCCACCAGCACCCAGCCACCCAGCGCCACCGCTGGCGAACACCGCATTCaggaagg                                                                                                                          >  1:1334747/1‑140 (MQ=255)                  tcaatcaaCGCCCGGCCCCGCTGCCGGGTTTTTGCTATGCACCACAATTACCCCAACCGGATACACAGCCGGATACAATTCCACCAGCACCCAGCCACCCAGCGCCACCGCTGGCGAACACCGCATTCaggaa                                                                                                                            >  1:974155/1‑133 (MQ=255)                  tcaatcaaCGCCCGGCCCCGCTGCCGGGTTTTTGCTATGCACCACAATTACCCCAACCGGACACACAGCCGGATACAATTCCACCAGCACCCAGCCACCCAGCGCCACCGCTGGCGAACACCGCATTCaggaa                                                                                                                            <  2:974155/133‑1 (MQ=255)                   caatcaaCGCCCGGCCCCGCTGCCGGGTTTTTGCTATGCACCACAATTACCCCAACCGGATACACAGCCGGATACAATTCCACCAGCACCCAGCCACCCAGCGCCACCGCTGGCGAACACCGCATTCAGGAAGGAAATGc                                                                                                                    >  1:1279508/1‑140 (MQ=255)                   caatcaaCGCCCGGCCCCGCTGCCGGGTTTTTGCTATGCACCACAATTACCCCAACCGGATACACAGCCGGATACAATTCCACCAGCACCCAGCCACCCAGCGCCACCGCTGGCGAACACCGCATTCAGGAAGGAAATGc                                                                                                                    >  2:1339266/1‑140 (MQ=255)                    aatcaaCGCCCGGCCCCGCTGCCGGGTTTTTGCTATGCACCACAATTACCCCAACCGGATACACAGCCGGATACAATTCCACCAGCACCCAGCCACCCAGCGCCACCGCTGGCGAACACCGCATTCAGGAAGGAAATGCg                                                                                                                   <  2:1334747/140‑1 (MQ=255)                       caaCGCCCGGCCCCGCTGCCGGGTTTTTGCTATGCACCACAATTACCCCAACCGGATACACAGCCGGATACAATTCCACCAGCACCCAGCCACCCAGCGCCACCGCTGGCGAACACCGCATTCAGGAAGGAAATGCGAGt                                                                                                                >  1:512735/1‑140 (MQ=255)                              cGGCCCCGCTGCCGGGTTTTTGCTATGCACCACAATTACCCCAACCGGATACACAGCCGGATACAATTCCACCAGCACCCAGCCACCCAGCGCCACCGCTGGCGAACACCGCATTCAGGAAGGAAATGCGAGTGATTtca                                                                                                         >  1:1337932/1‑140 (MQ=255)                               ggCCCCGCTGCCGGGTTTTTGCTATGCACCACAATTACCCCAACCGGATACACAGCCGGATACAATTCCACCAGCACCCAGCCACCCAGCGCCACCGCTGGCGAACACCGCATTCAGGAAGGAAATGCGAGTGATTtcac                                                                                                        >  2:172602/1‑140 (MQ=255)                                gCCCCGCTGCCGGGTTTTTGCTATGCACCACAATTACCCCAACCGGATACACAGCCGGATACAATTCCACCAGCACCCAGCCACCCAGCGCCACCGCTGGCGAACACCGCATTCAGGAAGGAAATGCGAGTGATTtcacc                                                                                                       >  1:1021672/1‑140 (MQ=255)                                   ccGCTGCCGGGTTTTTGCTATGCACCACAATTACCCCAACCGGATACACAGCCGGATACAATTCCACCAGCACCCAGCCACCCAGCGCCACCGCTGGCGAACACCGCATTCAGGAAGGAAATGCGAGTGATTtcaccgtc                                                                                                    <  2:708984/140‑1 (MQ=255)                                    cGCTGCCGGGTTTTTGCTATGCACCACAATTACCCCAACCGGATACACAGCCGGATACAATTCCACCAGCACCCAGCCACCCAGCGCCACCGCTGGCGAACACCGCATTCAGGAAGGAAATGCGAGTGATTtcaccgtca                                                                                                   <  2:1043595/140‑1 (MQ=255)                                       tGCCGGGTTTTTGCTATGCACCACAATTACCCCAACCGGATACACAGCCGGATACAATTCCACCAGCACCCAGCCACCCAGCGCCACCGCTGGCGAACACCGCATTCAGGAAGGAAATGCGAGTGATTtcaccgtcaccg                                                                                                <  1:321482/140‑1 (MQ=255)                                        gCCGGGTTTTTGCTATGCACCACAATTACCCCAACCGGATACACAGCCGGATACAATTCCACCAGCACCCAGCCACCCAGCGCCACCGCTGGCGAACACCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCgg                                                                                               <  2:1374969/140‑1 (MQ=255)                                            ggTTTTTGCTATGCACCACAATTACCCCAACCGGATACACAGCCGGATACAATTCCACCAGCACCCAGCCACCCAGCGCCACCGCTGGCGAACACCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGcaca                                                                                           <  2:1385490/140‑1 (MQ=255)                                                tttGCTATGCACCACAATTACCCCAACCGGATACACAGCCGGATACAATTCCACCAGCACCCAGCCACCCAGCGCCACCGCTGGCGAACACCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCg                                                                                       >  1:1280920/1‑140 (MQ=255)                                                 ttGCTATGCACCACAATTACCCCAACCGGATACACAGCCGGATACAATTCCACCAGCACCCAGCCACCCAGCGCCACCGCTGGCGAACACCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGa                                                                                      >  2:549724/1‑140 (MQ=255)                                                      aTGCACCACAATTACCCCAACCGGATACACAGCCGGATACAATTCCACCAGCACCCAGCCACCCAGCGCCACCGCTGGCGAACACCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGc                                                                                 >  1:48198/1‑140 (MQ=255)                                                             acaATTACCCCAACCGGATACACAGCCGGATACAATTCCACCAGCACCCAGCCACCCAGCGCCACCGCTGGCGAACACCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAag                                                                          <  1:172602/140‑1 (MQ=255)                                                             acaATTACCCCAACCGGATACACAGCCGGATACAATTCCACCAGCACCCAGCCACCCAGCGCCACCGCTGGCGAACACCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAag                                                                          <  2:1168938/140‑1 (MQ=255)                                                              caATTACCCCAACCGGATACACAGCCGGATACAATTCCACCAGCACCCAGCCACCCAGCGCCACCGCTGGCGAACACCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGAt                                                                                     >  1:1072784/1‑128 (MQ=255)                                                              caATTACCCCAACCGGATACACAGCCGGATACAATTCCACCAGCACCCAGCCACCCAGCGCCACCGCTGGCGAACACCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGAt                                                                                     <  2:1072784/128‑1 (MQ=255)                                                               aaTTACCCCAACCGGATACACAGCCGGATACAATTCCACCAGCACCCAGCCACCCAGCGCCACCGCTGGCGAACACCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAagag                                                                        <  2:1014889/140‑1 (MQ=255)                                                               aaTTACCCCAACCGGATACACAGCCGGATACAATTCCACCAGCACCCAGCCACCCAGCGCCACCGCTGGCGAACACCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAagag                                                                        <  2:1021672/140‑1 (MQ=255)                                                                aTTACCCCAACCGGATACACAGCCGGATACAATTCCACCAGCACCCAGCCACCCAGCGCCACCGCTGGCGAACACCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAgg                                                                       <  2:594904/140‑1 (MQ=255)                                                                 ttACCCCAACCGGATACACAGCCGGATACAATTCCACCAGCACCCAGCCACCCAGCGCCACCGCTGGCGAACACCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGc                                                                      >  2:490721/1‑140 (MQ=255)                                                                     cccAACCGGATACACAGCCGGATACAATTCCACCAGCACCCAGCCACCCAGCGCCACCGCTGGCGAACACCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTAc                                                                  <  2:970126/140‑1 (MQ=255)                                                                      ccAACCGGATACACAGCCGGATACAATTCCACCAGCACCCAGCCACCCAGCGCCACCGCTGGCGAACACCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTAcc                                                                 <  2:335332/140‑1 (MQ=255)                                                                      ccAACCGGATACACAGCCGGATACAATTCCACCAGCACCCAGCCACCCAGCGCCACCGCTGGCGAACACCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTAcc                                                                 <  1:549724/140‑1 (MQ=255)                                                                         aCCGGATACACAGCCGGATACAATTCCACCAGCACCCAGCCACCCAGCGCCACCGCTGGCGAACACCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAAt                                                              >  2:1030793/1‑140 (MQ=255)                                                                         aCCGGATACACAGCCGGATACAATTCCACCAGCACCCAGCCACCCAGCGCCACCGCTGGCGAACACCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAAt                                                              >  2:1367656/1‑140 (MQ=255)                                                                         aCCGGATACACAGCCGGATACAATTCCACCAGCACCCAGCCACCCAGCGCCACCGCTGGCGAACACCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAAt                                                              >  2:1119981/1‑140 (MQ=255)                                                                           cGGATACACAGCCGGATACAATTCCACCAGCACCCAGCCACCCAGCGCCACCGCTGGCGAACACCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGt                                                            <  1:1339266/140‑1 (MQ=255)                                                                           cGGATACACAGCCGGATACAATTCCACCAGCACCCAGCCACCCAGCGCCACCGCTGGCGAACACCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGt                                                            <  1:602572/140‑1 (MQ=255)                                                                              aTACACAGCCGGATACAATTCCACCAGCACCCAGCCACCCAGCGCCACCGCTGGCGAACACCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGt                                                            >  1:460498/1‑137 (MQ=255)                                                                              aTACACAGCCGGATACAATTCCACCAGCACCCAGCCACCCAGCGCCACCGCTGGCGAACACCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGt                                                            <  2:460498/137‑1 (MQ=255)                                                                               tACACAGCCGGATACAATTCCACCAGCGACCAGCCACCCAGCGCCACCGCTGGCGAACACCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGACCCGCCAAAAAGAGGCGTACCAATGt                                                            <  2:735552/136‑1 (MQ=255)                                                                               tACACAGCCGGATACAATTCCACCAGCACCCAGCCACCCAGCGCCACCGCTGGCGAACACCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGt                                                            >  1:735552/1‑136 (MQ=255)                                                                               tACACAGCCGGATACAATTCCACCAGCACCCAGCCACCCAGCGCCACCGCTGGCGAACACCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGat                                                        >  1:1395133/1‑140 (MQ=255)                                                                                 cacaGCCGGATACAATTCCACCAGCACCCAGCCACCCAGCGCCACCGCTGGCGAACACCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGatat                                                      <  2:141309/140‑1 (MQ=255)                                                                                     gCCGGATACAATTCCACCAGCACCCAGCCACCCAGCGCCACCGCTGGCGAACACCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAGTc                                                  >  1:643314/1‑140 (MQ=255)                                                                                       cGGATACAATTCCACCAGCACCCAGCCACCCAGCGCCACCGCTGGCGAACACCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCaa                                                               >  1:928526/1‑125 (MQ=255)                                                                                       cGGATACAATTCCACCAGCACCCAGCCACCCAGCGCCACCGCTGGCGAACACCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCaa                                                               <  2:928526/125‑1 (MQ=255)                                                                                                ttCCACCAGCACCCAGCCACCCAGCGCCACCGCTGGCGAACACCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAGTCCCCCGCGCGGa                                       >  2:227340/1‑140 (MQ=255)                                                                                                    accaGCACCCAGCCACCCAGCGCCACCGCTGGCGAACACCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAGTCCCCCGCGCGGACAAt                                   >  1:396165/1‑140 (MQ=255)                                                                                                    accaGCACCCAGCCACCCAGCGCCACCGCTGGCGAACACCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAGTCCCCCGCGCGGACAAt                                   <  1:1030793/140‑1 (MQ=255)                                                                                                        gCACCCAGCCACCCAGCGCCACCGCTGGCGAACACCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAGTCCCCCGCGCGGACAATCACt                               >  1:143780/1‑140 (MQ=255)                                                                                                          acccagccacccagcGCCACCGCTGGCGAACACCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAGTCCCCCGCGCGGACAATCACTTc                             >  1:163880/1‑140 (MQ=255)                                                                                                            ccagccacccagcGCCACCGCTGGCGAACACCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAGTCCCCCGCGCGGACAATCACTTCGt                           <  2:48198/140‑1 (MQ=255)                                                                                                                        cgcCACCGCTGGCGAACACCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAGTCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCa               <  2:396165/140‑1 (MQ=255)                                                                                                                              cGCTGGCGAACACCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCGCCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAGTCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGa         <  1:715865/140‑1 (MQ=255)                                                                                                                              cGCTGGCGAACACCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAGTCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGa         <  2:512735/140‑1 (MQ=255)                                                                                                                                cTGGCGAACACCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAGTCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAgt       <  1:490721/140‑1 (MQ=255)                                                                                                                                cTGGCGAACACCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAGTCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAgt       <  2:1395133/140‑1 (MQ=255)                                                                                                                                cTGGCGAACACCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAGTCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAg        <  1:389231/139‑1 (MQ=255)                                                                                                                                cTGGCGAACACCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAGTCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAg        >  2:389231/1‑139 (MQ=255)                                                                                                                                     gAACACCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAGTCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGttgttg  <  1:1367656/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         CCTTCAACCCCATCTCATCAATCAACGCCCGGCCCCGCTGCCGGGTTTTTGCTATGCACCACAATTACCCCAACCGGATACACAGCCGGATACAATTCCACCAGCACCCAGCCACCCAGCGCCACCGCTGGCGAATACCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAGTCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTG  >  NC_000913/2565338‑2565609

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑