Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,605,854	T→C	G348G (GGT→GGC)	hyfD →	hydrogenase 4 component D

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,605,854	0	T	C	100.0%	176.9 / NA	49	G348G (GGT→GGC)	hyfD	hydrogenase 4 component D
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/27);  total (22/27)

GACCATCACTCAACTTGGCTGGATGTTCTTCGGCTTGTCGCTCTCCATCTTCGGCTCGCGGCTGGCGCTGGAGGGCAGCATCGCCTACATCGTCAACCACGCGTTCGCTAAAAGCCTGTTTTTCCTTGTAGCAGGTGCGCTGAGTTACAGCTGCGGCACGCGCTTGTTGCCGCGTCTGCGTGGCGTATTGCACACCCTGCCGTTGCCAGGCGTGGGTTTCTGCGTGGCAGCGCTGGCGATTACCGGCGTGCCGCCGTTCAACGGCTTC  >  NC_000913/2605719‑2605986                                                                                                                                        |                                                                                                                                     gACCATCACTCAACTTGGCTGGATGTTCTTCGGCTTGTCGCTCTCCATCTTCGGCTCGCGGCTGGCGCTGGAGGGCAGCATCGCCTACATCGTCAACCACGCGTTCGCTAAAAGCCTGTTTTTCCTTGTAGCAGgcgcgc                                                                                                                                  <  2:665694/140‑1 (MQ=255)      tCACTCAACTTGGCTGGATGTTCTTCGGCTTGTCGCTCTCCATCTTCGGCTCGCGGCTGGCGCTGGAGGGCAGCATCGCCTACATCGTCAACCACGCGTTCGCTAAAAGCCTGTTTTTCCTTGTAGCAGGCGCGCTGAGt                                                                                                                             <  1:66436/140‑1 (MQ=255)        aCTCAACTTGGCTGGATGTTCTTCGGCTTGTCGCTCTCCATCTTCGGCTCGCGGCTGGCGCTGGAGGGCAGCATCGCCTACATCGTCAACCACGCGTTCGCTAAAAGCCTGTTTTTCCTTGTAGCAGGCGCGCTGAGTTa                                                                                                                           >  2:672579/1‑140 (MQ=255)            aaCTTGGCTGGATGTTCTTCGGCTTGTCGCTCTCCATCTTCGGCTCGCGGCTGGCGCTGGAGGGCAGCATCGCCTACATCGTCCACCACGCGTTCGCTAAAAGCCTGTTTTTCCTTGTAGCAGGCGCGCTGAGTTACAGc                                                                                                                       >  2:876348/1‑140 (MQ=255)                 ggCTGGATGTTCTTCGGCTTGTCGCTCTCCATCTTCGGCTCGCGGCTGGCGCTGGAGGGCAGCATCGCCTACATCGTCAACCACGCGTTCGCTAAAAGCCTGTTTTTCCTTGTAGCAGGCGCGCTGAGt                                                                                                                             >  2:52918/1‑129 (MQ=255)                 ggCTGGATGTTCTTCGGCTTGTCGCTCTCCATCTTCGGCTCGCGGCTGGCGCTGGAGGGCAGCATCGCCTACATCGTCAACCACGCGTTCGCTAAAAGCCTGTTTTTCCTTGTAGCAGGCGCGCTGAGt                                                                                                                             <  1:52918/129‑1 (MQ=255)                 ggCTGGATGTTCTTCGGCTTGTCGCTCTCCATCTTCGGCTCGCGGCTGGCGCTGGAGGGCAGCATCGCCTACATCGTCAACCACGCGTTCGCTAAAAGCCTGTTTTTCCTTGTAGCAGGCGCGCTGAGTTACAGCTGCgg                                                                                                                  >  2:80844/1‑140 (MQ=255)                 ggCTGGATGTTCTTCGGCTTGTCGCTCTCCATCTTCGGCTCGCGGCTGGCGCTGGAGGGCAGCATCGCCTACATCGTCAACCACGCGTTCGCTAAAAGCCTGTTTTTCCTTGTAGCAGGCGCGCTGAGTTACAGCTGCgg                                                                                                                  >  1:317593/1‑140 (MQ=255)                 ggCTGGATGTTCTTCGGCTTGTCGCTCTCCATCTTCGGCTCGCGGCTGGCGCTGGAGGGCAGCATCGCCTACATCGTCAACCACGCGTTCGCTAAAAGCCTGTTTTTCCTTGTAGCAGGCGCGCTGAGTTACAGCTGCgg                                                                                                                  >  2:841779/1‑140 (MQ=255)                  gCTGGATGTTCTTCGGCTTGTCGCTCTCCATCTTCGGCTCGCGGCTGGCGCTGGAGGGCAGCATCGCCTACATCGTCAACCACGCGTTCGCTAAAAGCCTGTTTTTCCTTGTAGCAGGCGCGCTGAGTTACAGCTGCGGc                                                                                                                 <  1:1077131/140‑1 (MQ=255)                    tGGATGTTCTTCGGCTTGTCGCTCTCCATCTTCGGCTCGCGGCTGGCGCTGGAGGGCAGCATCGCCTACATCGTCAACCACGCGTTCGCTAAAAGCCTGTTTTTCCTTGTAGCAGGCGCGCTGAGTTACAGCTGCGGCAc                                                                                                               <  2:843958/140‑1 (MQ=255)                    tGGATGTTCTTCGGCTTGTCGCTCTCCATCTTCGGCTCGCGGCTGGCGCTGGAGGGCAGCATCGCCTACATCGTCAACCACGCGTTCGCTAAAAGCCTGTTTTTCCTTGTAGCAGGCGCGCTGAGTTACAGCTGCGGCAc                                                                                                               <  2:1308298/140‑1 (MQ=255)                    tGGATGTTCTCCGGCTTGTCGCTCTCCATCTTCGGCTCGCGGCTGGCGCTGGAGGGCAGCATCGCCTACATCGTCAACCACGCGTTCGCTAAAAGCCTGTTTTTCCTTGTAGCAGGCGCGCTGAGTTACAGCTGCGGCAc                                                                                                               <  1:958338/140‑1 (MQ=255)                        tGTTCTTCGGCTTGTCGCTCTCCATCTTCGGCTCGCGGCTGGCGCTGGAGGGCAGCATCGCCTACATCGTCAACCACGCGTTCGCTAAAAGCCTGTTTTTCCTTGTAGCAGGCGCGCTGAGTTACAGCTGCGGCACgcgc                                                                                                           >  2:1367524/1‑140 (MQ=255)                        tGTTCTTCGGCTTGTCGCTCTCCATCTTCGGCTCGCGGCTGGCGCTGGAGGGCAGCATCGCCTACATCGTCAACCACGCGTTCGCTAAAAGCCTGTTTTTCCTTGTAGCAGGCGCGCTGAGTTACAGCTGCGGCACgcgc                                                                                                           >  2:913774/1‑140 (MQ=255)                        tGTTCTTCGGCTTGTCGCTCTCCATCTTCGGCTCGCGGCTGGCGCTGGAGGGCAGCATCGCCTACATCGTCAACCACGCGTTCGCTAAAAGCCTGTTTTTCCTTGTAGCAGGCGCGCTGAGTTACAGCTGCGGCACgcgc                                                                                                           <  2:776693/140‑1 (MQ=255)                               cGGCTTGTCGCTCTCCATCTTCGGCTCGCGGCTGGCGCTGGAGGGCAGCATCGCCTACATCGTCAACCACGCGTTCGCTAAAAGCCTGTTTTTCCTTGTAGCAGGCGCGCTGAGTTACAGCTGCGGCACGCGCTTGTTGc                                                                                                    <  1:1299659/140‑1 (MQ=255)                                 gCTTGTCGCTCTCCATCTTCGGCTCGCGGCTGGCGCTGGAGGGCAGCATCGCCTACATCGTCAACCACGCGTTCGCTAAAAGCCTGTTTTTCCTTGTAGCAGGCGCGCTGAGTTACAGCTGCGGCACGCGCTTGTTGCcg                                                                                                  <  1:876348/140‑1 (MQ=255)                                   ttGTCGCTCTCCATCTTCGGCTCGCGGCTGGCGCTGGAGGGCAGCATCGCCTACATCGTCAACCACGCGTTCGCTAAAAGCCTGTTTTTCCTTGTAGCAGGCGCGCTGAGTTACAGCTGCGGCACGCGCTTGTTGCcgcg                                                                                                <  1:1367524/140‑1 (MQ=255)                                         ctctCCATCTTCGGCTCGCGGCTGGCGCTGGAGGGCAGCATCGCCTACATCGTCAACCACGCGTTCGCTAAAAGCCTGTTTTTCCTTGTAGCAGGCGCGCTGAGTTACAGCTGCGGCACGCGCTTGTTGCCGCGTCTGCg                                                                                          <  1:819819/140‑1 (MQ=255)                                           ctcCATCTTCGGCTCGCGGCTGGCGCTGGAGGGCAGCATCGCCTACATCGTCAACCACGCGTTCGCTAAAAGCCTGTTTTTCCTTGTAGCAGGCGCGCTGAGTTACAGCTGCGGCACGCGCTTGTTGCCGCGTCTGCGTg                                                                                        <  2:365698/140‑1 (MQ=255)                                           ctcCATCTTCGGCTCGCGGCTGGCGCTGGAGGGCAGCATCGCCTACATCGTCAACCACGCGTTCGCTAAAAGCCTGTTTTTCCTTGTAGCAGGCGCGCTGAGTTACAGCTGCGGCACGCGCTTGTTGCCGCGTCTGCGTg                                                                                        <  2:1363195/140‑1 (MQ=255)                                           ctcCATCTTCGGCTCGCGGCTGGCGCTGGAGGGCAGCATCGCCCACATCGTCAACCACGCGTTCGCTAAAAGCCTGTTTTTCCTTGTAGCAGGCGCGCTGAGTTACAGCTGCGGCACGCGCTTGTTGCCGCGTCTGCGTg                                                                                        <  2:268901/140‑1 (MQ=255)                                             ccATCTTCGGCTCGCGGCTGGCGCTGGAGGGCAGCATCGCCTACATCGTCAACCACGCGTTCGCTAAAAGCCTGTTTTTCCTTGTAGCAGGCGCGCTGAGTTACAGCTGCGGCACGCGCTTGTTGCCGCGTCTGCGTGGc                                                                                      <  2:1108444/140‑1 (MQ=255)                                              cATCTTCGGCTCGCGGCTGGCGCTGGAGGGCAGCATCGCCTACATCGTCAACCACGCGTTCGCTAAAATCCTGTTTTTCCTTGTAGCAGGCGCGCTGAGTTACAGCTGCGGCACGCGCTTGTTGCCGCGTCTGCGTGGCg                                                                                     <  2:974829/140‑1 (MQ=255)                                              cATCTTCGGCTCGCGGCTGGCGCTGGAGGGCAGCATCGCCTACATCGTCAACCACGCGTTCGCTAAAAGCCTGTTTTTCCTTGTAGCAGGCGCGCTGAGTTACAGCTGCGGCACGCGCTTGTTGCCGCGTCTGCGTGGCg                                                                                     <  1:672579/140‑1 (MQ=255)                                                      gCTCGCGGCTGGCGCTGGAGGGCAGCATCGCCTACATCGTCAACCACGCGTTCGCTAAAAGCCTGTTTTTCCTTGTAGCAGGCGCGCTGAGTTACAGCTGCGGCACGCGCTTGTTGCCGCGTCTGCGTGGCGTATTGcac                                                                             >  1:290661/1‑140 (MQ=255)                                                         cgcgGCTGGCGCTGGAGGGCAGCATCGCCTACATCGTCAACCACGCGTTCGCTAAAAGCCTGTTTTTCCTTGTAGCAGGCGCGCTGAGTTACAGCTGCGGCACGCGCTTGTTGCCGCGTCTGCGTGGCGTATTGCACAcc                                                                          <  1:284130/140‑1 (MQ=255)                                                              cTGGCGCTGGAGGGCAGCATCGCCTACATCGTCAACCACGCGTTCGCTAAAAGCCTGTTTTTCCTTGTAGCAGGCGCGCTGAGTTACAGCTGCGGCACGCGCTTGTTGCCGCGTCTGCGTGGCGTATTGCACACCCTGcc                                                                     <  2:210257/140‑1 (MQ=255)                                                                  cgcTGGAGGGCAGCATCGCCTACATCGTCAACCACGCGTTCGCTAAAAGCCTGTTTTTCCTTGTAGCAGGCGCGCTGAGTTACAGCTGCGGCACGCGCTTGTTGCCGCGTCTGCGTGGCGTATTGCACACCCTGCCGTTg                                                                 <  2:1242070/140‑1 (MQ=255)                                                                            cagcaTCGCCTACATCGTCAACCACGCGTTCGCTAAAAGCCTGTTTTTCCTTGTAGCAGGCGCGCTGAGTTACAGCTGCGGCACGCGCTTGTTGCCGCGTCTGCGTGGCGTATTGCACACCCTGCCGTTGCCAGGCGTgg                                                       >  2:691322/1‑140 (MQ=255)                                                                            cagcaTCGCCTACATCGTCAACCACGCGTTCGCTAAAAGCCTGTTTTTCCTTGTAGCAGGCGCGCTGAGTTACAGCTGCGGCACGCGCTTGTTGCCGCGTCTGCGTGGCGTATTGCACACCCTGCCGTTGCCAGGCGTgg                                                       <  1:80844/140‑1 (MQ=255)                                                                              gcaTCGCCTACATCGTCAACCACGCGTTCGCTAAAAGCCTGTTTTTCCTTGTAGCAGGCGCGCTGAGTTACAGCTGCGGCACGCGCTTGTTGCCGCGTCTGCGTGGCGTATTGCACACCCTGCCGTTGCCAGGCGTGGGt                                                     <  1:241773/140‑1 (MQ=255)                                                                              gcaTCGCCTACATCGTCAACCACGCGTTCGCTAAAAGCCTGTTTTTCCTTGTAGCAGGCGCGCTGAGTTACAGCTGCGGCACGCGCTTGTTGCCGCGTCTGCGTGGCGTATTGCACACCCTGCCGTTGCCAGGCGTGGGt                                                     <  2:403108/140‑1 (MQ=255)                                                                                 tCGCCTACATCGTCAACCACGCGTTCGCTAAAAGCCTGTTTTTCCTTGTAGCAGGCGCGCTGAGTTACAGCTGCGGCACGCGCTTGTTGCCGCGTCTGCGTGGCGTATTGCACACCCTGCCGTTGCCAGGCGTGGGTTTc                                                  >  1:616948/1‑140 (MQ=255)                                                                                    ccTACATCGTCAACCACGCGTTCGCTAAAAGCCTGTTTTTCCTTGTAGCAGGCGCGCTGAGTTACAGCTGCGGCACGCGCTTGTTGCCGCGTCTGCGTGGCGTATTGCACACCCTGCCGTTGCCAGGCGTGGGTTTCTGc                                               >  2:914328/1‑140 (MQ=255)                                                                                       aCATCGTCAACCACGCGTTCGCTAAAAGCCTGTTTTTCCTTGTAGCAGGCGCGCTGAGTTACAGCTGCGGCACGCGCTTGTTGCCGCGTCTGCGTGGCGTATTGCACACCCTGCCGTTGCCAGGCGTGGGTTTCTGCGTg                                            >  2:1279094/1‑140 (MQ=255)                                                                                        cATCGTCAACCACGCGTTCGCTAAAAGCCTGTTTTTCCTTGTAGCAGGCGCGCTGAGTTACAGCTGCGGCACGCGCTTGTTGCCGCGTCTGCGTGGCGTATTGCACACCCTGCCGTTGCCAGGCGTGGGTTTCTGCGTgg                                           <  1:837112/140‑1 (MQ=255)                                                                                         aTCGTCAACCACGCGTTCGCTAAAAGCCTGTTTTTCCTTGTAGCAGGCGCGCTGAGTTACAGCTGCGGCACGCGCTTGTTGCCGCGTCTGCGTGGCGTATTGCACACCCTGCCGTTGCCAGGCGTGGGTTTCTGCGTGGc                                          >  2:108342/1‑140 (MQ=255)                                                                                           cGTCAACCACGCGTTCGCTAAAAGCCTGTTTTTCCTTGTAGCAGGCGCGCTGAGTTACAGCTGCGGCACGCGCTTGTTGCCGCGTCTGCGTGGCGTATTGCACACCCTGCCGTTGCCAGGCGTGGGTTTCTGCGTGGCAg                                        >  1:563457/1‑140 (MQ=255)                                                                                                  cACGCGTTCGCTAAAAGCCTGGTTTTCCTTGTAGCAGGCGCGCTGAGTTACAGCTGCGGCACGCGCTTGTTGCCGCGTCTGCGTGGCGTATTGCACACCCTGCCGTTGCCAGGCGTGGGTTTCTGCGTGGCAGCGCTGGc                                 >  2:943000/1‑140 (MQ=255)                                                                                                   aCGCGTTCGCTAAAAGCCTGTTTTTCCTTGTAGCAGGCGCGCTGAGTTACAGCTGCGGCACGCGCTTGTTGCCGCGTCTGCGTGGCGTATTGCACACCCTGCCGTTGCCAGGCGTGGGTTTCTGCGTGGCAGCGCTGGCg                                >  2:1343458/1‑140 (MQ=255)                                                                                                    cgcgTTCGCTAAAAGCCTGTTTTTCCTTGTAGCAGGCGTGCTGAGTTACAGCTGCGGCACGCGCTTGTTGCCGCGTCTGCGTGGCGTATTGCACACCCTGCCGTTGCCAGGCGTGGGTTTCTGCGTGGCAGCGCTg                                   <  2:129148/136‑1 (MQ=255)                                                                                                    cgcgTTCGCTAAAAGCCTGTTTTTCCTTGTAGCAGGCGCGCTGAGTTACAGCTGCGGCACGCGCTTGTTGCCGCGTCTGCGTGGCGTATTGCACACCCTGCCGTTGCCAGGCGTGGGTTTCTGCGTGGCAGCGCTg                                   >  1:129148/1‑136 (MQ=255)                                                                                                    cgcgTTCGCTAAAAGCCTGTTTTTCCTTGTAGCAGGCGCGCTGAGTTACAGCTGCGGCACGCGCTTGTTGCCGCGTCTGCGTGGCGTATTGCACACCCTGCCGTTGCCAGGCGTGGGTTTCTGCGTGGCAGCGCTGGCGa                               >  1:569589/1‑140 (MQ=255)                                                                                                      cgTTCGCTAAAAGCCTGTTTTTCCTTGTAGCAGGCGCGCTGAGTTACAGCTGCGGCACGCGCTTGTTGCCGCGTCTGCGTGGCGTATTGCACACCCTGCCGTTGCCAGGCGTGGGTTTCTGCGTGGCAGCGCTGGCGAtt                             >  1:401832/1‑140 (MQ=255)                                                                                                       gTTCGCTAAAAGCCTGTTTTTCCTTGTAGCAGGCGCGCTGAGTTACAGCTGCGGCACGCGCTTGTTGCCGCGTCTGCGTGGCGTATTGCACACCCTGCCGTTGCCAGGCGTGGGTTTCTGCGTGGCAGCGCTGGCGATTa                            >  2:340721/1‑140 (MQ=255)                                                                                                                              ttGTAGCAGGCGCGCTGAGTTACAGCTGCGGCACGCGCTTGTTGCCGCGTCTGCGTGGCGTATTGCACACCCTGCCGTTGCCAGGCGTGGGTTTCTGCGTGGCAGCGCTGGCGATTACCGGCGTGCCGCCGTTCAACGGc     <  2:290661/140‑1 (MQ=255)                                                                                                                               tGTAGCAGGCGCGCTGAGTTACAGCTGCGGCACGCGCTTGTTGCCGCGTCTGCGTGGCGTATTGCACACCCTGCCGTTGCCAGGCGTGGGTTTCTGCGTGGCAGCGCTGGCGATTACCGGCGTGCCGCCGTTCAACGGct    <  2:317593/140‑1 (MQ=255)                                                                                                                                 tAGCAGGCGCGCTGAGTTACAGCTGCGGCACGCGCTTGTTGCCGCGTCTGCGTGGCGTATTGCACACCCTGCCGTTGCCAGGCGTGGGTTTCTGCGTGGCAGCGCTGGCGATTACCGGCGTGCCGCCGTTCAACGGcttc  >  2:614763/1‑140 (MQ=255)                                                                                                                                 tAGCAGGCGCGCTGAGTTACAGCTGCGGCACGCGCTTGTTGCCGCGTCTGCGTGGCGTATTGCACACCCTGCCGTTGCCAGGCGTGGGTTTCTGCGTGGCAGCGCTGGCGATTACCGGCGTGCCGCCGTTCAACGGcttc  >  1:915050/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                     GACCATCACTCAACTTGGCTGGATGTTCTTCGGCTTGTCGCTCTCCATCTTCGGCTCGCGGCTGGCGCTGGAGGGCAGCATCGCCTACATCGTCAACCACGCGTTCGCTAAAAGCCTGTTTTTCCTTGTAGCAGGTGCGCTGAGTTACAGCTGCGGCACGCGCTTGTTGCCGCGTCTGCGTGGCGTATTGCACACCCTGCCGTTGCCAGGCGTGGGTTTCTGCGTGGCAGCGCTGGCGATTACCGGCGTGCCGCCGTTCAACGGCTTC  >  NC_000913/2605719‑2605986

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑