Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,623,206	G→A	A55T (GCT→ACT)	ppk →	polyphosphate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,623,206	0	G	A	100.0%	307.6 / NA	87	A55T (GCT→ACT)	ppk	polyphosphate kinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (48/39);  total (48/39)

AAAAGAGCTCAGTTGGTTATCGTTCAATGAACGCGTGCTTCAGGAAGCGGCGGACAAATCTAACCCGCTGATTGAAAGGATGCGTTTCCTGGGGATCTATTCCAATAACCTTGATGAGTTCTATAAAGTCCGCTTCGCTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTATTGC  >  NC_000913/2623070‑2623341                                                                                                                                         |                                                                                                                                        aaaaGAGCTCAGTTGGTTATCGTTCAATGAACGCGTGCTTCAGGAAGCGGCGGACAAATCTAACCCGCTGATTGAAAGGATGCGTTTCCTGGGGATCTATTCCAATAACCTTGATGAGTTCTATAAAGTCCGCTTCActg                                                                                                                                      <  1:547335/140‑1 (MQ=255) aaaaGAGCTCAGTTGGTTATCGTTCAATGAACGCGTGCTTCAGGAAGCGGCGGACAAATCTAACCCGCTGATTGAAAGGATGCGTTTCCTGGGGATCTATTCCAATAACCTTGATGAGTTCTATAAAGTCCGCTTCActg                                                                                                                                      <  2:345757/140‑1 (MQ=255)         tCAGTTGGTTATCGTTCAATGAACGCGTGCTTCAGGAAGCGGCGGACAAATCTAACCCGCTGATTGAAAGGATGCGTTTCCTGGGGATCTATTCCAATAACCTTGATGAGTTCTATAAAGTCCGCTTCACTGAACTGAAg                                                                                                                              <  1:436585/140‑1 (MQ=255)           aGTTGGTTATCGTTCAATGAACGCGTGCTTCAGGAAGCGGCGGACAAATCTAACCCGCTGATTGAAAGGATGCGTTTCCTGGGGATCTATTCCAATAACCTTGATGAGTTCTATAAAGTCCGCTTCACTGAACTGAAGCg                                                                                                                            >  1:1342112/1‑140 (MQ=255)           aGTTGGTTATCGTTCAATGAACGCGTGCTTCAGGAAGCGGCGGACAAATCTAACCCGCTGATTGAAAGGATGCGTTTCCTGGGGATCTATTCCAATAACCTTGATGAGTTCTATAAAGTCCGCTTCACTGAACTGAAGCg                                                                                                                            >  2:1151052/1‑140 (MQ=255)            gTTGGTTATCGTTCAATGAACGCGTGCTTCAGGAAGCGGCGGACAAATCTAACCCGCTGATTGAAAGGATGCGTTTCCTGGGGATCTATTCCAATAACCTTGATGAGTTCTATAAAGTCCGCTTCACTGAACTGAAGCGa                                                                                                                           <  2:875937/140‑1 (MQ=255)             ttGGTTATCGTTCAATGAACGCGTGCTTCAGGAAGCGGCGGACAAATCTAACCCGCTGATTGAAAGGATGCGTTTCCTGGGGATCTATTCCAATAACCTTGATGAGTTCTATAAAGTCCGCTTCACTGAACTGAAGCGAc                                                                                                                          <  1:1151052/140‑1 (MQ=255)                gTTATCGTTCAATGAACGCGTGCTTCAGGAAGCGGCGGACAAATCTAACCCGCTGATTGAAAGGATGCGTTTCCTGGGGATCTATTCCAATAACCTTGATGAGTTCTATAAAGTCCGCTTCACTGAACTGAAGCGACGca                                                                                                                       <  1:918001/140‑1 (MQ=255)                 ttATCGTTCAATGAACGCGTGCTTCAGGAAGCGGCGGACAAATCTAACCCGCTGATTGAAAGGATGCGTTTCCTGGGGATCTATTCCAATAACCTTGATGAGTTCTATAAAGTCCGCTTCACTGAACTGAAGCGACGcat                                                                                                                      <  2:1269349/140‑1 (MQ=255)                    tCGTTCAATGAACGCGTGCTTCAGGAAGCGGCGGACAAATCTAACCCGCTGATTGAAAGGATGCGTTTCCTGGGGATCTATTCCAATAACCTTGATGAGTTCTATAAAGTCCGCTTCACTGAACTGAAGCGACGcatcat                                                                                                                   <  1:527528/140‑1 (MQ=255)                       ttCAATGAACGCGTGCTTCAGGAAGCGGCGGACAAATCTAACCCGCTGATTGAAAGGATGCGTTTCCTGGGGATCTATTCCAATAACCTTGATGAGTTCTATAAAGTCCGCTTCACTGAACTGAAGCGAc                                                                                                                          >  2:1344548/1‑130 (MQ=255)                       ttCAATGAACGCGTGCTTCAGGAAGCGGCGGACAAATCTAACCCGCTGATTGAAAGGATGCGTTTCCTGGGGATCTATTCCAATAACCTTGATGAGTTCTATAAAGTCCGCTTCACTGAACTGAAGCGAc                                                                                                                          <  1:1344548/130‑1 (MQ=255)                       ttCAATGAACGCGTGCTTCAGGAAGCGGCGGACAAATCTAACCCGCTGATTGAAAGGATGCGTTTCCTGGGGATCTATTCCAATAACCTTGATGAGTTCTATAAAGTCCGCTTCACTGAACTGAAGCGACGCATCattat                                                                                                                >  2:657700/1‑140 (MQ=255)                       ttCAATGAACGCGTGCTTCAGGAAGCGGCGGACAAATCTAACCAGCTGATTGAAAGGATGCGTTTCCTGGGGATCTATTCCAATAACCTTGATGAGTTCTATAAAGTCCGCTTCACTGAACTGAAGCGa                                                                                                                           >  1:984027/1‑129 (MQ=255)                       ttCAATGAACGCGTGCTTCAGGAAGCGGCGGACAAATCTAACCAGCTGATTGAAAGGATGCGTTTCCTGGGGATCTATTCCAATAACCTTGATGAGTTCTATAAAGTCCGCTTCACTGAACTGAAGCGa                                                                                                                           <  2:984027/129‑1 (MQ=255)                            tGAACGCGTGCTTCAGGAAGCGGCGGACAAATCTAACCCGCTGATTGAAAGGATGCGTTTCCTGGGGATCTATTCCAATAACCTTGATGAGTTCTATAAAGTCCGCTTCACTGAACTGAAGCGACGCATCATTATTAGCg                                                                                                           <  1:1134106/140‑1 (MQ=255)                            tGAACGCGTGCTTCAGGAAGCGGCGGACAAATCTAACCCGCTGATTGAAAGGATGCGTTTCCTGGGGATCTATTCCAATAACCTTGATGAGTTCTATAAAGTCCGCTTCACTGAACTGAAGCGACGCATCATTATTAGCg                                                                                                           >  1:1077858/1‑140 (MQ=255)                                    tGCTTCAGGAAGCGGCGGACAAATCTAACCCGCTGATTGAAAGGATGCGTTTCCTGGGGATCTATTCCAATAACCTTGATGAGTTCTATAAAGTCCGCTTCACTGAACTGAAGCGACGCATCATTATTAGCGAAGAACaa                                                                                                   <  2:1342112/140‑1 (MQ=255)                                    tGCTTCAGGAAGCGGCGGACAAATCTAACCCGCTGATTGAAAGGATGCGTTTCCTGGGGATCTATTCCAATAACCTTGATGAGTTCTATAAAGTCCGCTTCACTGAACTGAAGCGACGCATCATTATTAGCGAAGAACaa                                                                                                   >  1:1188034/1‑140 (MQ=255)                                    tGCTTCAGGAAGCGGCGGACAAATCTAACCCGCTGATTGAAAGGATGCGTTTCCTGGGGATCTATTCCAATAACCTTGATGAGTTCTATAAAGTCCGCTTCACTGAACTGAAGCGACGCATCATTATTAGCGAAGAACaa                                                                                                   <  1:1037271/140‑1 (MQ=255)                                     gCTTCAGGAAGCGGCGGACAAATCTAACCCGCTGATTGAAAGGATGCGTTTCCTGGGGATCTATTCCAATAACCTTGATGAGTTCTATAAAGTCCGCTTCACTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAg                                                                                                  >  1:696450/1‑140 (MQ=255)                                         cAGGAAGCGGCGGACAAATCTAACCCGCTGATTGAAAGGATGCGTTTCCTGGGGATCTATTCCAATAACCTTGATGAGTTCTATAAAGTCCGCTTCACTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTc                                                                                              <  1:668100/140‑1 (MQ=255)                                          aGGAAGCGGCGGACAAATCTAACCCGCTGATTGAAAGGATGCGTTTCCTGGGGATCTATTCCAATAACCTTGATGAGTTCTATAAAGTCCGCTTCACTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTcc                                                                                             <  1:909835/140‑1 (MQ=255)                                                 ggcggACAAATCTAACCCGCTGATTGAAAGGATGCGTTTCCTGGGGATCTATTCCAATAACCTTGATGAGTTCTATAAAGTCCGCTTCACTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACtctc                                                                                      >  2:160433/1‑140 (MQ=255)                                                      aCAAATCTAACCCGCTGATTGAAAGGATGCGTTTCCTGGGGATCTATTCCAATAACCTTGATGAGTTCTATAAAGTCCGCTTCACTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTc                                                                                              >  1:1173682/1‑127 (MQ=255)                                                      aCAAATCTAACCCGCTGATTGAAAGGATGCGTTTCCTGGGGATCTATTCCAATAACCTTGATGAGTTCTATAAAGTCCGCTTCACTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTc                                                                                              <  2:1173682/127‑1 (MQ=255)                                                      aCAAATCTAACCCGCTGATTGAAAGGATGCGTTTCCTGGGGATCTATTCCAATAACCTTGATGAGTTCTATAAAGTCCGCTTCACTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTcc                                                                                 <  1:657700/140‑1 (MQ=255)                                                          aTCTAACCCGCTGATTGAAAGGATGCGTTTCCTGGGGATCTATTCCAATATCCTTGATGAGTTCTATAAAGTCCGCTTCACTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGcc                                                                             >  2:905997/1‑140 (MQ=255)                                                                 ccGCTGATTGAAAGGATGCGTTTCCTGGGGATCTATTCCAATAACCTTGATGAGTTCTATAAAGTCCGCTTCACTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACt                                                                      <  2:1188034/140‑1 (MQ=255)                                                                  cGCTGATTGAAAGGATGCGTTTCCTGGGGATCTATTCCAATAACCTTGATGAGTTCTATATAGTCCGCTTCACTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTg                                                                     >  1:27361/1‑140 (MQ=255)                                                                  cGCTGATTGAAAGGATGCGTTTCCTGGGGATCTATTCCAATAACCTTGATGAGTTCTATAAAGTCCGCTTCACTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTg                                                                     >  1:1169226/1‑140 (MQ=255)                                                                  cGCTGATTGAAAGGATGCGTTTCCTGGGGATCTATTCCAATAACCTTGATGAGTTCTATAAAGTCCGCTTCACTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTg                                                                     >  1:1302981/1‑140 (MQ=255)                                                                   gCTGATTGAAAGGATGCGTTTCCTGGGGATCTATTCCAATAACCTTGATGAGTTCTATAAAGTCCGCTTCACTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTgg                                                                    >  1:134435/1‑140 (MQ=255)                                                                    cTGATTGAAAGGATGCGTTTCCTGGGGATCTATTCCAATAACCTTGATGAGTTCTATAAAGTCCGCTTCACTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTggg                                                                   >  2:274020/1‑140 (MQ=255)                                                                    cTGATTGAAAGGATGCGTTTCCTGGGGATCTATTCCAATAACCTTGATGAGTTCTATAAAGTCCGCTTCACTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTggg                                                                   >  1:223480/1‑140 (MQ=255)                                                                      gATTGAAAGGATGCGTTTCCTGGGGATCTATTCCAATAACCTTGATGAGTTCTATAAAGTCCGCTTCACTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCa                                                                 <  2:1155925/140‑1 (MQ=255)                                                                      gATTGAAAGGATGCGTTTCCTGGGGATCTATTCCAATAACCTTGATGAGTTCTATAAAGTCCGCTTCACTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCa                                                                 <  1:905997/140‑1 (MQ=255)                                                                       aTTGAAAGGATGCGTTTCCTGGGGATCTATTCCAATAACCTTGATGAGTTCTATAAAGTCCGCTTCACTGAACTGAAGCGCCGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCaa                                                                >  1:285992/1‑140 (MQ=255)                                                                             aGGATGCGTTTCCTGGGGATCTATTCCAATAACCTTGATGAGTTCTATAAAGTCCGCTTCACTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCa                                                          <  2:1302981/140‑1 (MQ=255)                                                                             aGGATGCGTTTCCTGGGGATCTATTCCAATAACCTTGATGAGTTCTATAAAGTCCGCTTCACTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCa                                                          <  1:416065/140‑1 (MQ=255)                                                                                 tGCGTTTCCTGGGGATCTATTCCAATAACCTTGATGAGTTCTATAAAGTCCGCTTCACTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTcc                                                      >  2:448749/1‑140 (MQ=255)                                                                                  gCGTTTCCTGGGGATCTATTCCAATAACCTTGATGAGTTCTATAAAGTCCGCTTCACTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTccc                                                     >  1:3611/1‑140 (MQ=255)                                                                                  gCGTTTCCTGGGGATCTATTCCAATAACCTTGATGAGTTCTATAAAGTCCGCTTCACTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTccc                                                     >  2:148822/1‑140 (MQ=255)                                                                                       tCCTGGGGATCTATTCCAATAACCTTGATGAGTTCTATAAAGTCCGCTTCACTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTg                                                <  2:134435/140‑1 (MQ=255)                                                                                         cTGGGGATCTATTCCAATAACCTTGATGAGTTCTATAAAGTCCGCTTCACTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCt                                              >  2:375441/1‑140 (MQ=255)                                                                                          tGGGGATCTATTCCAATAACCTTGATGAGTTCTATAAAGTCCGCTTCACTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTg                                             >  2:989093/1‑140 (MQ=255)                                                                                          tGGGGATCTATTCCAATAACCTTGATGAGTTCTATAAAGTCCGCTTCACTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTg                                             >  1:395037/1‑140 (MQ=255)                                                                                           ggggATCTATTCCAATAACCTTGATGAGTTCTATAAAGTCCGCTTCACTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGa                                            >  2:327459/1‑140 (MQ=255)                                                                                              gATCTATTCCAATAACCTTGATGAGTTCTATAAAGTCCGCTTCACTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAg                                         <  2:395037/140‑1 (MQ=255)                                                                                                  tATTCCAATAACCTTGATGAGTTCTATAAAGTCCGCTTCACTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGaa                                           <  1:408827/134‑1 (MQ=255)                                                                                                  tATTCCAATAACCTTGATGAGTTCTATAAAGTCCGCTTCACTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGaa                                           >  2:408827/1‑134 (MQ=255)                                                                                                  tATTCCAATAACCTTGATGAGTTCTATAAAGTCCGCTTCACTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGa                                     >  1:308351/1‑140 (MQ=255)                                                                                                  tATTCCAATAACCTTGATGAGTTCTATAAAGTCCGCTTCACTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGa                                     >  1:1230850/1‑140 (MQ=255)                                                                                                        aaTAACCTTGATGAGTTCTATAAAGTCCGCTTCACTGAACTGAAGCGATGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGa                               >  1:427020/1‑140 (MQ=255)                                                                                                        aaTAACCTTGATGAGTTCTATAAAGTCCGCTTCACTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTGAGTCCCGGGTGCTGAAAGCCGATCAGGa                               >  1:599012/1‑140 (MQ=255)                                                                                                        aaTAACCTTGATGAGTTCTATAAAGTCCGCTTCACTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGa                               >  1:182774/1‑140 (MQ=255)                                                                                                         aTAACCTTGATGAGTTCTATAAAGTCCGCTTCACTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCg                                      >  2:623258/1‑132 (MQ=255)                                                                                                         aTAACCTTGATGAGTTCTATAAAGTCCGCTTCACTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCg                                      <  1:623258/132‑1 (MQ=255)                                                                                                         aTAACCTTGATGAGTTCTATAAAGTCCGCTTCACTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGaa                              >  2:1242423/1‑140 (MQ=255)                                                                                                         aTAACCTTGATGAGTTCTATAAAGTCCGCTTCACTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGaa                              >  1:537160/1‑140 (MQ=255)                                                                                                             ccTTGATGAGTTCTATAAAGTCCGCTTCACTGAACTGAAGCGATGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCg                          <  2:427020/140‑1 (MQ=255)                                                                                                              cTTGATGAGTTCTATAAAGTCCGCTTCACTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCg                          <  1:756434/139‑1 (MQ=255)                                                                                                              cTTGATGAGTTCTATAAAGTCCGCTTCACTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCg                          >  2:756434/1‑139 (MQ=255)                                                                                                              cTTGATGAGTTCTATAAAGTCCGCTTCACTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCGa                         <  1:274020/140‑1 (MQ=255)                                                                                                               ttGATGAGTTCTATAAAGTCCGCTTCACTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCGAc                        <  1:427200/140‑1 (MQ=255)                                                                                                               ttGATGAGTTCTATAAAGTCCGCTTCACTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCGAc                        <  1:448749/140‑1 (MQ=255)                                                                                                                 gatgaGTTCTATAAAGTCCGCTTCACTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCGACgg                      >  1:1300557/1‑140 (MQ=255)                                                                                                                  atgaGTTCTATAAAGTCCGCTTCACTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCGACGGc                     >  2:588565/1‑140 (MQ=255)                                                                                                                  atgaGTTCTATAAAGTCCGCTTCACTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCGACGGc                     >  1:125726/1‑140 (MQ=255)                                                                                                                  atgaGTTCTATAAAGTCCGCTTCACTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCGACGGc                     <  1:588565/140‑1 (MQ=255)                                                                                                                        tCTATAAAGTCCGCTTCACTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCGACGGCCTCTAc               >  1:201762/1‑140 (MQ=255)                                                                                                                          tataAAGTCCGCTTCACTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACaa             <  2:3611/140‑1 (MQ=255)                                                                                                                            taAAGTCCGCTTCACTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACg           <  2:1077858/140‑1 (MQ=255)                                                                                                                              aaGTCCGCTTCACTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAg         <  2:1300557/140‑1 (MQ=255)                                                                                                                               aGTCCGCTTCACTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGc        <  2:537160/140‑1 (MQ=255)                                                                                                                                 tCCGCTTCACTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGc        <  1:306026/138‑1 (MQ=255)                                                                                                                                 tCCGCTTCACTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGc        >  2:306026/1‑138 (MQ=255)                                                                                                                                 tCCGCTTCACTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTa      >  2:1209278/1‑140 (MQ=255)                                                                                                                                 tCCGCTTCACTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTa      >  1:203766/1‑140 (MQ=255)                                                                                                                                 tCCGCTTCACTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTa      >  1:407931/1‑140 (MQ=255)                                                                                                                                 tCCGCTTCACTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTa      >  1:550485/1‑140 (MQ=255)                                                                                                                                 tCCGCTTCACTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTa      >  2:1366089/1‑140 (MQ=255)                                                                                                                                 tCCGCTTCACTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTa      <  2:201762/140‑1 (MQ=255)                                                                                                                                    gCTTCACTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTATTg   >  2:955136/1‑140 (MQ=255)                                                                                                                                    gCTTCACTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTATTg   <  1:1242423/140‑1 (MQ=255)                                                                                                                                     cTTCACTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTATTGc  >  1:1278203/1‑140 (MQ=255)                                                                                                                                     cTTCACTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTATTGc  >  1:1356357/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        AAAAGAGCTCAGTTGGTTATCGTTCAATGAACGCGTGCTTCAGGAAGCGGCGGACAAATCTAACCCGCTGATTGAAAGGATGCGTTTCCTGGGGATCTATTCCAATAACCTTGATGAGTTCTATAAAGTCCGCTTCGCTGAACTGAAGCGACGCATCATTATTAGCGAAGAACAAGGCTCCAACTCTCATTCCCGCCATTTACTGGGCAAAATTCAGTCCCGGGTGCTGAAAGCCGATCAGGAATTCGACGGCCTCTACAACGAGCTATTGC  >  NC_000913/2623070‑2623341

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑