Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	346,972	G→A	A491V (GCT→GTT)	prpR ←	DNA‑binding transcriptional dual regulator PrpR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	346,972	0	G	A	100.0%	215.4 / NA	61	A491V (GCT→GTT)	prpR	DNA‑binding transcriptional dual regulator PrpR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/33);  total (28/33)

TCGCGCTGCATTCGGCAGTTCAGCTTTTCAGCCGCCGCCAGAACGTCGTCCGGCTGATGCCTAAATAATTCGCCGCTGCTGTTTTATCGCCATTAAATTTCTCCAGTGCCTGTTGTGGTGTCAGTAAGCGTGGAGCGGGAGTTTTCGCCGACTCGCGCGCCAGTTCCGGCAGTAGCAGTTGCATAAACTGCGGCGTTAAATCCGGCGTCGGTTCCACACTTAAAAACAGCGCCAGTCGTTCCATCATATTGCGCAGTTCACGAATATTGC  >  NC_000913/346838‑347107                                                                                                                                       |                                                                                                                                        tCGCGCTGCATTCGGCAGTTCAGCTTTTCAGCCGCCGCCAGAACGTCGTCCGGCTGATGCCTAAATAATTCGCCGCTGCTGTTTTATCGCCATTAAATTTCTCCAGTGCCTGTTGTGGTGTCAGTAAGCGTGGAACGGGa                                                                                                                                    >  2:479847/1‑140 (MQ=255)  cgcgCTGCATTCGGCAGTTCAGCTTTTCAGCCGCCGCCAGAACGTCGTCCGGCTGATGCCTAAATAATTCGCCGCTGCTGTTTTATCGCCATTAAATTTCTCCAGTGCCTGTTGTGGTGTCAGTAAGCGTGGAACGGGAg                                                                                                                                   >  2:992256/1‑140 (MQ=255)  cgcgCTGCATTCGGCAGTTCAGCTTTTCAGCCGCCGCCAGAACGTCGTCCGGCTGATGCCTAAATAATTCGCCGCTGCTGTTTTATCGCCATTAAATTTCTCCAGTGCCTGTTGTGGTGTCAGTAAGCGTGGAACGGGAg                                                                                                                                   >  1:1179165/1‑140 (MQ=255)                   ttCAGCTTTTCAGCCGCCGCCAGAACGTCGTTCGGCTGATGCCTAAATAATTCGCCGCTGCTGTTTTATCGCCATTAAATTTCTCCAGTGCCTGTTGTGGTGTCAGTAAGCGTGGAACGGGAGTTTTCGCCGACTcgcgc                                                                                                                  >  1:617214/1‑140 (MQ=255)                    tCAGCTTTTCAGCCGCCGCCAGAACGTCGTTCGGCTGATGCCTAAATAATTCGCCGCTGCTGTTTTATCGCCATTAAATTTCTCCAGTGCCTGTTGTGGTGTCAGTAAGCGTGGAACGGGAGTTTTCGCCGACTcgcgcg                                                                                                                 <  2:617214/140‑1 (MQ=255)                    tCAGCTTTTCAGCCGCCGCCAGAACGTCGTCCGGCTGATGCCTAAATAATTCGCCGCTGCTGTTTTATCGCCATTAAATTTCTCCAGTGCCTGTTGTGGTGTCAGTAAGCGTGGAACGGGAGTTTTCGCCGACTcgcgcg                                                                                                                 <  1:628995/140‑1 (MQ=255)                     cAGCTTTTCAGCCGCCGCCAGAACGTCGTCCGGCTGATGCCTAAATAATTCGCCGCTGCTGTTTTATCGCCATTAAATTTCTCCAGTGCCTGTTGTGGTGTCAGTAAGCGTGGAACGGGAGTTTTCGCCGACTCgcgcgc                                                                                                                >  2:314546/1‑140 (MQ=255)                        cTTTTCAGCCGCCGCCAGAACGTCGTCCGGCTGATGCCTAAATAATTCGCCGCTGCTGTTTTATCGCCATTAAATTTCTCCAGTGCCTGTTGTGGTGTCAGTAAGCGTGGAACGGGAGTTTTCGCCGACTCGCGCGCCAg                                                                                                             >  1:131423/1‑140 (MQ=255)                          tttCAGCCGCCGCCAGAACGTCGTCCGGCTGATGCCTAAATAATTCGCCGCTGCTGTTTTATCGCCATTAAATTTCTCCAGTGCCTGTTGTGGTGTCAGTAAGCGTGGAACGGGAGTTTTCGCCGACTCGCGCGCCAGtt                                                                                                           <  1:753985/140‑1 (MQ=255)                             cAGCCGCCGCCAGAACGTCGTCCGGCTGATGCCTAAATAATTCGCCGCTGCTGTTTTATCGCCATTAAATTTCTCCAGTGCCTGTTGTGGTGTCAGTAAGCGTGGAACGGGAGTTTTCGCCGACTCGCGCGCCAGTTCCg                                                                                                        <  1:87942/140‑1 (MQ=255)                                ccgccgccAGAACGTCGTCCGGCTGATGCCTAAATAATTCGCCGCTGCTGTTTTATCGCCATTAAATTTCTCCAGTGCCTGTTGTGGTGTCAGTAAGCGTGGAACGGGAGTTTTCGCCGACTCGCGCGCCAGCTCCGGCa                                                                                                     >  2:296814/1‑140 (MQ=255)                                    cgccAGAACGTCGTCCGGCTGATGCCTAAATAATTCGCCGCTGCTGTTTTATCGCCATTAAATTTCTCCAGTGCCTGTTGTGGTGTCAGTAAGCGTGGAACGGGAGTTTTCGCCGACTCGCGCGCCAGTTCCGGCAGTAg                                                                                                 <  2:803134/140‑1 (MQ=255)                                      ccAGAACGTCGTCCGGCTGATGCCTAAATAATTCGCCGCTGCTGTTTTATCGCCATTAAATTTCTCCAGTGCCTGTTGTGGTGTCAGTAAGCGTGGAACGGGAGTTTTCGCCGACTCGCGCGCCAGTTCCGGCAGTAGCa                                                                                               <  1:935105/140‑1 (MQ=255)                                       cAGAACGTCGTCCGGCTGATGCCTAAATAATTCGCCGCTGCTGTTTTATCGCCATTAAATTTCTCCAGTGCCTGTTGTGGTGTCAGTAAGCGTGGAACGGGAGTTTTCGCCGACTCGCGCGCCAGTTCCGGCAGTAGCAg                                                                                              <  2:777285/140‑1 (MQ=255)                                       cAGAACGTCGTCCGGCTGATGCCTAAATAATTCGCCGCTGCTGTTTTATCGCCATTAAATTTCTCCAGTGCCTGTTGTGGTGTCAGTAAGCGTGGAACGGGAGTTTTCGCCGACTCGCGCGCCAGTTCCGGCAGTAGCAg                                                                                              <  2:337831/140‑1 (MQ=255)                                           aCGTCGTCCGGCTGATGCCTAAATAATTCGCCGCTGCTGTTTTATCGCCATTAAATTTCTCCAGTGCCTGTTGTGGTGTCAGTAAGCGTGGAACGGGAGTTTTCGCCGACTCGCGCGCCAGTTCCGGCAGTAGCAGTTGc                                                                                          <  1:992256/140‑1 (MQ=255)                                                   cGGCTGATGCCTAAATAATTCGCCGCTGCTGTTTTATCGCCATTAAATTTCTCCAGTGCCTGTTGTGGTGTCAGTAAGCGTGGAACGGGAGTTTTCGCCGACTCGCGCGCCAGTTCCGGCAGTAGCAGTTGCATAAACTg                                                                                  <  1:101290/140‑1 (MQ=255)                                                   cGGCTGATGCCTAAATAATTCGCCGCTGCTGTTTTATCGCCATTAAATTTCTCCAGTGCCTGTTGTGGTGTCAGTAAGCGTGGAACGGGAGTTTTCGCCGACTCGCGCGCCAGTTCCGGCAGTAGCAGTTGCATAAACTg                                                                                  <  2:738306/140‑1 (MQ=255)                                                     gCTGATGCCTAAATAATTCGCCGCTGCTGTTTTATCGCCATTAAATTTCTCCAGTGCCTGTTGTGGTGTCAGTAAGCGTGGAACGGGAGTTTTCGCCGACTCGCGCGCCAGTTCCGGCAGTAGCAGTTGCATAAACTgcg                                                                                >  2:729812/1‑140 (MQ=255)                                                     gCTGATGCCTAAATAATTCGCCGCTGCTGTTTTATCGCCATTAAATTTCTCCAGTGCCTGTTGTGGTGTCAGTAAGCGTGGAACGGGAGTTTTCGCCGACTCGCGCGCCAGTTCCGGCAGTAGCAGTTGCATAAACTgcg                                                                                <  2:822381/140‑1 (MQ=255)                                                     gCTGATGCCTAAATAATTCGCCGCTGCTGTTTTATCGCCATTAAATTTCTCCAGTGCCTGTTGTGGTGTCAGTAAGCGTGGAACGGGAGTTTTCGCCGACTCGCGCGCCAGCTCCGGCAGTAGCAGTTGCATAAACTgcg                                                                                <  1:296814/140‑1 (MQ=255)                                                          tGCCTAAATAATTCGCCGCTGCTGTTTTATCGCCATTAAATTTCTCCAGTGCCTGTTGTGGTGTCAGTAAGCGTGGAACGGGAGTTTTCGCCGACTCGCGCGCCAGTTCCGGCAGTAGCAGTTGCATAAACTGCGGCGtt                                                                           >  1:788291/1‑140 (MQ=255)                                                           gCCTAAATAATTCGCCGCTGCTGTTTTATCGCCATTAAATTTCTCCAGTGCCTGTTGTGGTGTCAGTAAGCGTGGAACGGGAGTTTTCGCCGACTCGCGCGCCAGTTCCGGCAGTAGCAGTTGCATAAACTGCGGCGTTa                                                                          <  1:1065178/140‑1 (MQ=255)                                                                     ttCGCCGCTGCTGTTTTATCGCCATTAAATTTCTCTAGTGCCTGTTGTGGTGTCAGTAAGCGTGGAACGGGAGTTTTCGCCGACTCGAGCGCCAGTTCCGGCAGTAGCAGTTGCATAAACTGCGGCGTTAAAt                                                                       <  2:1305366/133‑1 (MQ=255)                                                                     ttCGCCGCTGCTGTTTTATCGCCATTAAATTTCTCCAGTGCCTGTTGTGGTGTCAGTAAGCGTGGAACGGGAGTTTTCGCCGACTCGCGCGCCAGTTCCGGCAGTAGCAGTTGCATAAACTGCGGCGTTAAAt                                                                       >  1:1305366/1‑133 (MQ=255)                                                                     ttCGCCGCTGCTGTTTTATCGCCATTAAATTTCTCCAGTGCCTGTTGTGGTGTCAGTAAGCGTGGAACGGGAGTTTTCGCCGACTCGCGCGCCAGTTCCGGCAGTAGCAGTTGCATAAACTGCGGCGTTAAATCCGGCGt                                                                >  1:680866/1‑140 (MQ=255)                                                                          cgcTGCTGTTTTATCGCCATTAAATTTCTCCAGTGCCTGTTGTGGTGTCAGTAAGCGTGGAACGGGAGTTTTCGCCGACTCGCGCGCCAGTTCCGGCAGTAGCAGTTGCATAAACTGCGGCGTTAAATCCGGCGTCGGtt                                                           >  2:980628/1‑140 (MQ=255)                                                                           gctgctGTTTTATCGCCATTAAATTTCTCCAGTGCCTGTTGTGGTGTCAGTAAGCGTGGAACGGGAGTTTTCGCCGACTCGCGCGCCAGTTCCGGCAGTAGCAGTTGCATAAACTGCGGCGTTAAATCCGGCGTCGGTTc                                                          <  1:729812/140‑1 (MQ=255)                                                                             tgctgTTTTATCGCCATTAAATTTCTCCAGTGCCTGTTGTGGTGTCAGTAAGCGTGGAACGGGAGTTTTCGCCGACTCGCGCGCCAGTTCCGGCAGTAGCAGTTGCATAAACTGCGGCGTTAAATCCGTCGTCGGTTCca                                                        >  1:1110073/1‑140 (MQ=255)                                                                             tgctgTTTTATCGCCATTAAATTTCTCCAGTGCCTGTTGTGGTGTCAGTAAGCGTGGAACGGGAGTTTTCGCCGACTCGCGCGCCAGTTCCGGCAGTAGCAGTTGCATAAACTGCGGCGTTAAATCCGGCGTCGGTTCca                                                        <  2:1179165/140‑1 (MQ=255)                                                                                    ttATCGCCATTAAATTTCTCCAGTGCCTGTTGTGGTGTCAGTAAGCGTGGAACGGGAGTTTTCGCCGACTCGCGCGCCAGTTCCGGCAGTAGCAGTTGCATAAACTGCGGCGTTAAATCCGGCGTCGGTTCca                                                        <  1:474774/133‑1 (MQ=255)                                                                                    ttATCGCCATTAAATTTCTCCAGTGCCTGTTGTGGTGTCAGTAAGCGTGGAACGGGAGTTTTCGCCGACTCGCGCGCCAGTTCCGGCAGTAGCAGTTGCATAAACTGCGGCGTTAAATCCGGCGTCGGTTCca                                                        >  2:474774/1‑133 (MQ=255)                                                                                       tCGCCATTAAATTTCTCCAGTGCCTGTTGTGGTGTCAGTAAGCGTGGAACGGGAGTTTTCGCCGACTCGCGCGCCAGTTCCGGCAGTAGCAGTTGCATAAACTGCGGCGTTAAATCCGGCGTCGGTTCCACACTTaaaaa                                              >  1:379603/1‑140 (MQ=255)                                                                                            aTTAAATTTCTCCAGTGCCTGTTGTGGTGTCAGTAAGCGTGGAACGGGAGTTTTCGCCGACTCGCGCGCCAGTTCCGGCAGTAGCAGTTGCATAAACTGCGGCGTTAAATCCGGCGTCGGTTCCACACTTAAAAACAgcg                                         >  2:288472/1‑140 (MQ=255)                                                                                            aTTAAATTTCTCCAGTGCCTGTTGTGGTGTCAGTAAGCGTGGAACGGGAGTTTTCGCCGACTCGCGCGCCAGTTCCGGCAGTAGCAGTTGCATAAACTGCGGCGTTAAATCCGGCGTCGGTTCCACACTTAAAAACAgcg                                         <  2:699314/140‑1 (MQ=255)                                                                                                 aTTTCTCCAGTGCCTGTTGTGGTGTCTGTAAGCGTGGAACGGGAGTTTTCGCCGACTCGCGCGCCAGTTCCGGCAGTAGCAGTTGCATAAACTGCGGCGTTAAATCCGGCGTCGGTTCCACACTTAAAAACAGCGCCAGt                                    <  1:980628/140‑1 (MQ=255)                                                                                                 aTTTCTCCAGTGCCTGTTGTGGTGTCAGTAAGCGTGGGACGGGAGTTTTCGCCGACTCGCGCGCCAGTTCCGGCAGTAGCAGTTGCATAAACTGCGGCGTTAAATCCGGCGTCGGTTCCACACTTAAAAACAGCGCCAGt                                    >  2:1235179/1‑140 (MQ=255)                                                                                                 aTTTCTCCAGTGCCTGTTGTGGTGTCAGTAAGCGTGGAACGGGAGTTTTCGCCGACTCGCGCGCCAGTTCCGGCAGTAGCAGTTGCATAAACTGCGGCGTTAAATCCGGCGTCGGTTCCACACTTAAAAACAGCGCCAGt                                    <  2:430532/140‑1 (MQ=255)                                                                                                 aTTTCTCCAGTGCCTGTTGTGGTGTCAGTAAGCGTGGAACGGGAGTTTTCGCCGACTCGCGCGCCAGTTCCGGCAGTAGCAGTTGCATAAACTGCGGCGTTAAATCCGGCGTCGGTTCCACACTTAAAAACAGCGCCAGt                                    <  1:1253236/140‑1 (MQ=255)                                                                                                   ttCTCCAGTGCCTGTTGTGGTGTCAGTAAGCGTGGAACGGGAGTTTTCGCCGACTCGCGCGCCAGTTCCGGCAGTAGCAGTTGCATAAACTGCGGCGTTAAATCCGGCGTCGGTTCCACACTTAAAAACAGCGCCAGTCg                                  <  2:76567/140‑1 (MQ=255)                                                                                                   ttCTCCAGTGCCTGTTGTGGTGTCAGTAAGCGTGGAACGGGAGTTTTCGCCGACTCGCGCGCCAGTTCCGGCAGTAGCAGTTGCATAAACTGCGGCGTTAAATCCGGCGTCGGTTCCACACTTAAAAACAGCGCCAGTCg                                  <  2:1229180/140‑1 (MQ=255)                                                                                                     ctcCAGTGCCTGTTGTGGTGTCAGTAAGCGTGGAACGGGAGTTTTCGCCGACTCGCGCGCCAGTTCCGGCAGTAGCAGTTGCATAAACTGCGGCGTTAAATCCGGCGTCGGTTCCACACTTAAAAACAGCGCCAGTCGtt                                <  1:1235179/140‑1 (MQ=255)                                                                                                     ctcCAGTGCCTGTTGTGGTGTCAGTAAGCGTGGAACGGGAGTTTTCGCCGACTCGCGCGCCAGTTCCGGCAGTAGCAGTTGCATAAACTGCGGCGTTAAATCCGGCGTCGGTTCCACACTTAAAAACAGCGCCAGTCGtt                                >  2:485321/1‑140 (MQ=255)                                                                                                          gTGCCTGTTGTGGTGTCAGTAAGCGTGGAACGGGAGTTTTCGCCGACTCGCGCGCCAGTTCCGGCAGTAGCAGTTGCATAAACTGCGGCGTTAAATCCGGCGTCGGTTCCACACTTAAAAACAGCGCCAGTCGTTCcatc                           >  2:805191/1‑140 (MQ=255)                                                                                                           tGCCTGTTGTGGTGTCAGTAAGCGTGGAACGGGAGTTTTCGCCGACTCGCGCGCCAGTTCCGGCAGTAGCAGTTGCATAAACTGCGGCGTTAAATCCGGCGTCGGTTCCACACTTAAAAACAGCGCCAGTCGTTCcatca                          >  1:955363/1‑140 (MQ=255)                                                                                                             ccTGTTGTGGTGTCAGTAAGCGTGGAACGGGAGTTTTCGCCGACTCGCGCGCCAGTTCCGGCAGTAGCAGTTGCATAAACTGCGGCGTTAAATCCGGCGTCGGTTCCACACTTAAAAACAGCGCCAGTCGTTCCATCata                        >  2:427727/1‑140 (MQ=255)                                                                                                                gttgtGGTGTCAGTAAGCGTGGAACGGGAGTTTTCGCCGACTCGCGCGCCAGTTCCGGCAGTAGCAGTTGCATAAACTGCGGCGTTAAATCCGGCGTCGGTTCCACACTTAAAAACAGCGCCAGt                                    >  2:764082/1‑125 (MQ=255)                                                                                                                gttgtGGTGTCAGTAAGCGTGGAACGGGAGTTTTCGCCGACTCGCGCGCCAGTTCCGGCAGTAGCAGTTGCATAAACTGCGGCGTTAAATCCGGCGTCGGTTCCACACTTAAAAACAGCGCCAGt                                    <  1:764082/125‑1 (MQ=255)                                                                                                                gttgtGGTGTCAGTAAGCGTGGAACGGGAGTTTTCGCCGACTCGCGCGCCAGTTCCGGCAGTAGCAGTTGCATAAACTGCGGCGTTAAATCCGGCGTCGGTTCCACACTTAAAAACAGCGCCAGTCGTTCCATCATATTg                     <  2:955363/140‑1 (MQ=255)                                                                                                                  tgtgGTGTCAGTAAGCGTGGAACGGGAGTTTTCGCCGACTCGCGCGCCAGTTCCGGCAGTAGCAGTTGCATAAACTGCGGCGTTAAATCCGGCGTCGGTTCCACACTTAAAAACAGCGCCAGTCGTTCCATCATATTgcg                   <  2:1372527/140‑1 (MQ=255)                                                                                                                  tgtgGTGTCAGTAAGCGTGGAACGGGAGTTTTCGCCGACTCGCGCGCCAGTTCCGGCAGTAGCAGTTGCATAAACTGCGGCGTTAAATCCGGCGTCGGTTCCACACTTAAAAACAGCGCCAGTCGTTCCATCATATTgcg                   <  1:526806/140‑1 (MQ=255)                                                                                                                  tgtgGTGTCAGTAAGCGTGGAACGGGAGTTTTCGCCGACTCGCGCGCCAGTTCCGGCAGTAGCAGTTGCATAAACTGCGGCGTTAAATCCGGCGTCGGTTCCACACTTAAAAACAGCGCCAGTCGTTCCATCATATTgcg                   <  1:805191/140‑1 (MQ=255)                                                                                                                   gtggtgTCAGTAAGCGTGGAACGGGAGTTTTCGCCGACTCGCGCGCCAGTTCCGGCAGTAGCAGTTGCATAAACTGCGGCGTTAAATCCGGCGTCGGTTCCACACTTAAAAACAGCGCCAGTCGTTCCATCATATTgcgc                  <  1:427727/140‑1 (MQ=255)                                                                                                                      gtgtCAGTAAGCGTGGAACGGGAGTTTTCGCCGACTCGCGCGCCAGTTCCGGCAGTAGCAGTTGCATAAACTGCGGCGTTAAATCCGGCGTCGGTTCCACACTTAAAAACAGCGCCAGTCGTTCCATCATATTGCGCAGt               >  1:822557/1‑140 (MQ=255)                                                                                                                       tgtCAGTAAGCGTGGAACGGGAGTTTTCGCCGACTCGCGCGCCAGTTCCGGCAGTAGCAGTTGCATAAACTGCGGCGTTAAATCCGGCGTCGGTTCCACACTTAAAAACAGCGCCAGTCGTTCCATCATATTGCGCAGtt              >  2:1354400/1‑140 (MQ=255)                                                                                                                         tCAGTAAGCGTGGAACGGGAGTTTTCGCCGACTCGCGCGCCAGTTCCGGCAGTAGCAGTTGCATAAACTGCGGCGTTAAATCCGGCGTCGGTTCCACACTTAAAAACAGCGCCAGTCGTTCCATCATATTGCGCAGTTCa            <  2:119431/140‑1 (MQ=255)                                                                                                                           aGTAAGCGTGGAACGGGAGTTTTCGCCGACTCGCGCGCCAGTTCCGGCAGTAGCAGTTGCATAAACTGCGGCGTTAAATCCGGCGTCGGTTCCACACTTAAAAACAGCGCCAGTCGTTCCATCATATTGCGCAGTTCACg          >  1:740172/1‑140 (MQ=255)                                                                                                                                gCGTGGAACGGGAGTTTTCGCCGACTCGCGCGCCAGTTCCGGCAGTAGCAGTTGCATAAACTGCGGCGTTAAATCCGGCGTCGGTTCCACACTTAAAAACAGCGCCAGTCGTTCCATCATATTgcgc                  >  2:781469/1‑127 (MQ=255)                                                                                                                                gCGTGGAACGGGAGTTTTCGCCGACTCGCGCGCCAGTTCCGGCAGTAGCAGTTGCATAAACTGCGGCGTTAAATCCGGCGTCGGTTCCACACTTAAAAACAGCGCCAGTCGTTCCATCATATTgcgc                  <  1:781469/127‑1 (MQ=255)                                                                                                                                 cGTGGAACGGGAGTTTTCGCCGACTCGCGCGCCAGTTCCGGCAGTAGCAGTTGCATAAACTGCGGCGTTAAATCCGGCGTCGGTTCCACACTTAAAAACAGCGCCAGTCGTTCCATCATATTGCGCAGTTCACGAATAtt    >  1:561839/1‑140 (MQ=255)                                                                                                                                 cGTGGAACGGGAGTTTTCGCCGACTCGCGCGCCAGTTCCGGCAGTAGCAGTTGCATAAACTGCGGCGTTAAATCCGGCGTCGGTTCCACACTTAAAAACAGCGCCAGTCGTTCCATCATATTGCGCAGTTCACGAATAtt    >  2:1358307/1‑140 (MQ=255)                                                                                                                                   tGGAACGGGAGTTTTCGCCGACTCGCGCGCCAGTTCCGGCAGTAGCAGTTGCATAAACTGCGGCGTTAAATCCGGCGTCGGTTCCACACTTAAAAACAGCGCCAGTCGTTCCATCATATTGCGCAGTTCACGAATATTGc  >  2:1347425/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                        TCGCGCTGCATTCGGCAGTTCAGCTTTTCAGCCGCCGCCAGAACGTCGTCCGGCTGATGCCTAAATAATTCGCCGCTGCTGTTTTATCGCCATTAAATTTCTCCAGTGCCTGTTGTGGTGTCAGTAAGCGTGGAGCGGGAGTTTTCGCCGACTCGCGCGCCAGTTCCGGCAGTAGCAGTTGCATAAACTGCGGCGTTAAATCCGGCGTCGGTTCCACACTTAAAAACAGCGCCAGTCGTTCCATCATATTGCGCAGTTCACGAATATTGC  >  NC_000913/346838‑347107

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑