Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,105,846	A→G	S45G (AGC→GGC)	nupG →	nucleoside:H(+) symporter NupG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,105,846	0	A	G	100.0%	305.0 / NA	83	S45G (AGC→GGC)	nupG	nucleoside:H(+) symporter NupG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (42/41);  total (42/41)

TTAACATGAATCTTAAGCTGCAGCTGAAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATATGTTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATAGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCA  >  NC_000913/3105709‑3105981                                                                                                                                          |                                                                                                                                        ttAACATGAATCTTAAGCTGCAGCTGAAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATATGTTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATGGc                                                                                                                                       >  1:733963/1‑140 (MQ=255) ttAACATGAATCTTAAGCTGCAGCTGAAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATATGTTTGTTACCCTGAAGTTTGACGATGCTTCTATTGGCGCAGTTTATGGc                                                                                                                                       >  2:476455/1‑140 (MQ=255)  tAACATGAATCTTAAGCTGCAGCTGAAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATATGTTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATGGCt                                                                                                                                      <  2:1327118/140‑1 (MQ=255)    aCATGAATCTTAAGCTGCAGCTGAAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATATGTTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATGGCTCa                                                                                                                                    <  1:1068477/140‑1 (MQ=255)    aCATGAATCTTAAGCTGCAGCTGAAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATATGTTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATGGCTCa                                                                                                                                    <  2:429978/140‑1 (MQ=255)          aTCTTAAGCTGCAGCTGAAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATATGTTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATGGCTCACTGGGt                                                                                                                              >  1:204698/1‑140 (MQ=255)          aTCTTAAGCTGCAGCTGAAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATATGTTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATGGCTCACTGGGt                                                                                                                              >  1:501316/1‑140 (MQ=255)              tAAGCTGCAGCTGAAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATATGTTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATGGCTCACTGGGTATCg                                                                                                                          <  1:1127076/140‑1 (MQ=255)                   tGCAGCTGAAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATATGTTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATGGCTCACTGGGTATCGCAGCg                                                                                                                     <  1:247651/140‑1 (MQ=255)                   tGCAGCTGAAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATATGTTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATGGCTCACTGGGTATCGCAGCg                                                                                                                     <  2:387668/140‑1 (MQ=255)                   tGCAGCTGAAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATATGTTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATGGCTCACTGGGTATCGCAGCg                                                                                                                     <  2:693090/140‑1 (MQ=255)                   tGCAGCTGAAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATATGTTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATGGCTCACTGGGTATCGCAGCg                                                                                                                     <  2:23692/140‑1 (MQ=255)                      aGCTGAAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATATGTTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATGGCTCACTGGGTATCGCAGCGGTc                                                                                                                  >  1:949824/1‑140 (MQ=255)                        cTGAAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATATGTTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATGGCTCACTGGGTATCGCAGCGGTCtt                                                                                                                <  2:707989/140‑1 (MQ=255)                        cTGAAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATATGTTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATGGCTCACTGGGTATCGCAGCGGTCtt                                                                                                                <  1:702813/140‑1 (MQ=255)                          gAAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATATGTTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATGGCTCACTGGGTATCGCAGCGGTCTTTa                                                                                                              >  2:1088653/1‑140 (MQ=255)                           aaaaTCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATATGTTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATGGCTCACTGGGTATCGCAGCGGTCTTTAt                                                                                                             >  2:388121/1‑140 (MQ=255)                               tCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATATGTTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATGGCTCACTGGGTATCGCAg                                                                                                                       <  2:272149/126‑1 (MQ=255)                               tCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATATGTTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATGGCTCACTGGGTATCGCAg                                                                                                                       >  1:272149/1‑126 (MQ=255)                                ccTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATATGTTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATGGCTCACTGGGTATCGCAGCGGTCTTTATGCCTg                                                                                                        <  1:122817/140‑1 (MQ=255)                                  tctctTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATATGTTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATGGCTCACTGGGTATCGCAGCGGTCTTTATGCCTgcg                                                                                                      >  1:661407/1‑140 (MQ=255)                                      ttttCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATATGTTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATGGCTCACTGGGTATCGCAGCGGTCTTTATGCCTg                                                                                                        <  1:1113613/134‑1 (MQ=255)                                      ttttCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATATGTTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATGGCTCACTGGGTATCGCAGCGGTCTTTATGCCTg                                                                                                        >  2:1113613/1‑134 (MQ=255)                                                ttCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATATGTTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATGGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATtgt                                                                                        >  1:663695/1‑140 (MQ=255)                                                  ctgtctgtGGGGAAGTTGGCTGACGACCCTCGGCTCCTATATGTTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATGGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTgg                                                                                      <  1:1088653/140‑1 (MQ=255)                                                   tgtctgtGGGGAAGTTGGCTGACGACCCTCGGCTCCTATATGTTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATGGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGc                                                                                     <  2:501316/140‑1 (MQ=255)                                                   tgtctgtGGGGAAGTTGGCTGACGACCCTCGGCTCCTATATGTTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATGGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGc                                                                                     <  1:585124/140‑1 (MQ=255)                                                     tctgtGGGTAAGTTGGCTGACGACCCTCGGCTCCTATATGTTTGTTACCCTGAAGTTTGACGGTGCCTCTATTGGCGCAGTTTATGGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCg                                                                                   <  1:149785/140‑1 (MQ=255)                                                     tctgtGGGGAAGTTGGCTGACGACCCTCGGCTCCTATATGTTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATGGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCg                                                                                   <  2:371626/140‑1 (MQ=255)                                                     tctgtGGGGAAGTTGGCTGACGACCCTCGGCTCCTATATGTTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATGGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCg                                                                                   >  2:719574/1‑140 (MQ=255)                                                       tgtgGGGAAGTTGGCTGACGACCCTCGGCTCCTATATGTTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATGGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGAc                                                                                 <  2:733963/140‑1 (MQ=255)                                                       tgtgGGGAAGTTGGCTGACGACCCTCGGCTCCTATATGTTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATGGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGAc                                                                                 >  2:615652/1‑140 (MQ=255)                                                          ggggAAGTTGGCTGACGACCCTCGGCTCCTATATGTTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATGGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACaaa                                                                              >  1:987487/1‑140 (MQ=255)                                                          ggggAAGTTGGCTGACGACCCTCGGCTCCTATATGTTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATGGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACaaa                                                                              >  1:985844/1‑140 (MQ=255)                                                          ggggAAGTTGGCTGACGACCCTCGGCTCCTATATGTTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATGGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACaaa                                                                              >  1:20709/1‑140 (MQ=255)                                                           gggAAGTTGGCTGACGACCCTCGGCTCCTATATGTTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATGGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAAt                                                                             <  2:1139316/140‑1 (MQ=255)                                                               aGTTGGCTGACGACCCTCGGCTCCTATATGTTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATGGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGtt                                                                         >  1:1353981/1‑140 (MQ=255)                                                                gTTGGCTGACGACCCTCGGCTCCTATATGTTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATGGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTa                                                                        <  2:317971/140‑1 (MQ=255)                                                                   ggCTGACGACCCTCGGCTCCTATATGTTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATGGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGt                                                                     >  1:501150/1‑140 (MQ=255)                                                                    gCTGACGACCCTCGGCTCCTATATGTTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATGGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTg                                                                    <  2:985844/140‑1 (MQ=255)                                                                     cTGACGACCCTCGGCTCCTATATGTTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATGGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGc                                                                   <  1:615652/140‑1 (MQ=255)                                                                         cgacCCTCGGCTCCTATATGTTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATGGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGaaa                                                               <  2:536252/140‑1 (MQ=255)                                                                          gacCCTCGGCTCCTATATGTTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATGGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAAt                                                              <  1:1144720/140‑1 (MQ=255)                                                                          gacCCTCGGCTCCTATATGTTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATGGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAAt                                                              <  1:1218551/140‑1 (MQ=255)                                                                          gacCCTCGGCTCCTATATGTTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATGGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAAt                                                              <  1:138411/140‑1 (MQ=255)                                                                          gacCCTCGGCTCCTATATGTTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATGGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAAt                                                              <  2:278315/140‑1 (MQ=255)                                                                          gacCCTCGGCTCCTATATGTTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATGGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAAt                                                              <  2:212522/140‑1 (MQ=255)                                                                           acCCTCGGCTCCTATATGTTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATGGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATg                                                             >  2:1258771/1‑140 (MQ=255)                                                                              cTCGGCTCCTATATGTTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATGGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGa                                                                 >  1:27789/1‑133 (MQ=255)                                                                              cTCGGCTCCTATATGTTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATGGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGa                                                                 <  2:27789/133‑1 (MQ=255)                                                                                     ccTATATGTTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATGGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGcc                                                   <  2:720582/140‑1 (MQ=255)                                                                                     ccTATATGTTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATGGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGcc                                                   <  1:128369/140‑1 (MQ=255)                                                                                         tatGTTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATGGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCAttt                                               <  2:663695/140‑1 (MQ=255)                                                                                             tttgttACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATGGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCca                                           >  1:85031/1‑140 (MQ=255)                                                                                             tttgttACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATGGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCca                                           >  1:809358/1‑140 (MQ=255)                                                                                             tttgttACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATGGCTCACTGGGTATCGCAGCGGTCTTTATGCCCGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCca                                           <  2:1247676/140‑1 (MQ=255)                                                                                             tttgttACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATGGCTCACTGGGTATCACAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCca                                           >  2:273771/1‑140 (MQ=255)                                                                                               tgttACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATGGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCcaca                                         <  2:755966/140‑1 (MQ=255)                                                                                                gttACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATGGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCacac                                        >  2:261476/1‑140 (MQ=255)                                                                                                gttACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATGGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCacac                                        >  2:862973/1‑140 (MQ=255)                                                                                                gttACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATGGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCacac                                        >  1:313232/1‑140 (MQ=255)                                                                                                gttACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATGGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCacac                                        >  1:1329735/1‑140 (MQ=255)                                                                                                gttACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATGGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCacac                                        >  1:678185/1‑140 (MQ=255)                                                                                                 ttACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATGGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACAcc                                       >  1:130778/1‑140 (MQ=255)                                                                                                   aCCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATGGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCAt                                     >  1:208297/1‑140 (MQ=255)                                                                                                   aCCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATGGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGAAAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCAt                                     >  1:947388/1‑140 (MQ=255)                                                                                                    cccTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATGGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCAtt                                    >  2:523412/1‑140 (MQ=255)                                                                                                     ccTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATGGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTg                                   >  2:1158403/1‑140 (MQ=255)                                                                                                      cTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATGGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGAAAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTgg                                  <  2:947388/140‑1 (MQ=255)                                                                                                       tGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATGGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGgc                                 >  1:1162855/1‑140 (MQ=255)                                                                                                        gAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATGGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGgcg                                <  2:591641/140‑1 (MQ=255)                                                                                                           gTTTGACGGTGCTTCTATTGGCGCAGTTTATGGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTa                             <  1:446860/140‑1 (MQ=255)                                                                                                                     gCTTCTATTGGCGCAGTTTATGGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGtt                   <  1:719574/140‑1 (MQ=255)                                                                                                                      cTTCTATTGGCGCAGTTTATGGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTc                  >  2:1201382/1‑140 (MQ=255)                                                                                                                            ttGGCGCAGTTTATGGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATggcg            <  1:1036812/140‑1 (MQ=255)                                                                                                                              ggCGCAGTTTATGGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATggcggc          >  2:881752/1‑140 (MQ=255)                                                                                                                               gcgcAGTTTATGGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGca         <  1:523412/140‑1 (MQ=255)                                                                                                                                cgcAGTTTATGGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGcac        >  2:62989/1‑140 (MQ=255)                                                                                                                                cgcAGTTTATGGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGcac        >  1:465963/1‑140 (MQ=255)                                                                                                                                 gcAGTTTATGGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGcaca       >  1:765601/1‑140 (MQ=255)                                                                                                                                     tttATGGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTc   >  1:1027862/1‑140 (MQ=255)                                                                                                                                     tttATGGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTc   >  1:450821/1‑140 (MQ=255)                                                                                                                                      ttATGGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCa  >  2:60586/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        TTAACATGAATCTTAAGCTGCAGCTGAAAATCCTCTCTTTTCTGCAGTTCTGTCTGTGGGGAAGTTGGCTGACGACCCTCGGCTCCTATATGTTTGTTACCCTGAAGTTTGACGGTGCTTCTATTGGCGCAGTTTATAGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCA  >  NC_000913/3105709‑3105981

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑