Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,144,425	T→C	Y239C (TAC→TGC)	hybA ←	hydrogenase 2 iron‑sulfur protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,144,425	0	T	C	100.0%	206.5 / NA	57	Y239C (TAC→TGC)	hybA	hydrogenase 2 iron‑sulfur protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (32/25);  total (32/25)

ACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAGGCACACCCGTCAGTACCAGAACCTGAGTACCGCCGCCCTCTTTCTCGCCGTACAGATGCGGATAATATTTCGGCACCGTATGCAGGTAAGTGTCGCCAGATTTCAGCGTCTGACGCGGATAGTGGTATTCGCTGCCAGGCTTCAGCGCCAGACGTTTTTTCGCCTCCGCCATCAGCTCTTCACGCGTACCGAAAATCACCGCGCCCGCCGGGCACACTTCTA  >  NC_000913/3144292‑3144560                                                                                                                                      |                                                                                                                                        aCCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAGGCACACCCGTCAGTACCAGAACCTGAGTACCGCCGCCCTCTTTCTCGCCGTACAGATGCGGATAATATTTCGGCACCGTATGCAGGCAAgtgt                                                                                                                                   <  2:94283/140‑1 (MQ=255) aCCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAGGCACACCCGTCAGTACCAGAACCTGAGTACCGCCGCCCTCTTTCTCGCCGTACAGATGCGGATAATATTTCGGCACCGTATGCAGGCAAgtgt                                                                                                                                   <  1:948958/140‑1 (MQ=255)             aTCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAGGCACACCCGTCAGTACCAGAACCTGAGTACCGCCGCCCTCTTTCTCGCCGTACAGATGCGGATAATATTTCGGCACCGTATGCAGGCAAGTGTCGCCAGATTTCa                                                                                                                       >  2:1309559/1‑140 (MQ=255)               cGTCCAGTTTCGGTAGGTCGAGATTTTCATAAGGCACACCCGTCAGTACCAGAACCTGAGTACCGCCGCCCTCTTTCTCGCCGTACAGATGCGGATAATATTTCGGCACCGTATGCAGGCAAGTGTCGCCAGATTTCAGc                                                                                                                     >  2:412625/1‑140 (MQ=255)               cGTCCAGTTTCGGCAGGTCGAGATTTTCATAAGGCACACCCGTCAGTACCAGAACCTGAGTACCGCCGCCCTCTTTCTCGCCGTACAGATGCGGATAATATTTCGGCACCGTATGCAGGCAAGTGTCGCCAGATTTCAGc                                                                                                                     >  1:1344042/1‑140 (MQ=255)                gTCCAGTTTCGGCAGGTCGAGATTTTCATAAGGCACACCCGTCCGTACCAGAACCTGAGTACCGCCGCCCTCTTTCTCGCCGTACAGATGCGGATAATATTTCGGCACCGTATGCAGGCAAGTGTCGCCAGATTTCAGCg                                                                                                                    >  1:348940/1‑140 (MQ=255)                gTCCAGTTTCGGCAGGTCGAGATTTTCATAAGGCACACCCGTCAGTACCAGAACCTGAGTACCGCCGCCCTCTTTCTCGCCGTACAGATGCGGATAATATTTCGGCACCGTATGCAGGCAAGTGTCGCCAGAttt                                                                                                                         >  2:1321476/1‑135 (MQ=255)                gTCCAGTTTCGGCAGGTCGAGATTTTCATAAGGCACACCCGTCAGTACCAGAACCTGAGTACCGCCGCCCTCTTTCTCGCCGTACAGATGCGGATAATATTTCGGCACCGTATGCAGGCAAGTGTCGCCAGAttt                                                                                                                         <  1:1321476/135‑1 (MQ=255)                 tCCAGTTTCGGCAGGTCGAGATTTTCATAAGGCACACCCGTCAGTACCAGAACCTGAGTACCGCCGCCCTCTTTCTCGCCGTACAGATGCGGATAATATTTCGGCACCGTATGCAGGCAAGTGTCGCCAGATTTCAGCGt                                                                                                                   <  1:844548/140‑1 (MQ=255)                 tCCAGTTTCGGCAGGTCGAGATTTTCATAAGGCACACCCGTCAGTACCAGAACCTGAGTACCGCCGCCCTCTTTCTCGCCGTACAGATGCGGATAATATTTCGGCACCGTATGCAGGCAAGTGTCGCCAGATTTCAGCGt                                                                                                                   >  1:841325/1‑140 (MQ=255)                      tttCGGCAGGTCGAGATTTTCATAAGGCACACCCGTCAGTACCAGAACCTGAGTACCGCCGCCCTCTTTCTCGCCGTACAGATGCGGATAATATTTCGGCACCGTATGCAGGCAAGTGTCGCCAGATTTCAGCGTCTGAc                                                                                                              <  1:540566/140‑1 (MQ=255)                         cGGCAGGTCGAGATTTTCATAAGGCACACCCGTCAGTACCAGAACCTGAGTACCGCCGCCCTCTTTCTCGCCGTACAGATGCGGATAATATTTCGGCACCGTATGCAGGCAAGTGTCGCCAGATTTCAGCGTCTGAcgcg                                                                                                           <  1:890958/140‑1 (MQ=255)                               gTCGAGATTTTCATAAGGCACACCCGTCAGTACCAGAACCTGAGTACCGCCGCCCTCTTTCTCGCCGTACAGATGCGGATAATATTTCGGCACCGTATGCAGGCAAGTGTCGCCAGATTTCAGCGTCTGAc                                                                                                              <  2:580250/131‑1 (MQ=255)                               gTCGAGATTTTCATAAGGCACACCCGTCAGTACCAGAACCTGAGTACCGCCGCCCTCTTTCTCGCCGTACAGATGCGGATAATATTTCGGCACCGTATGCAGGCAAGTGTCGCCAGATTTCAGCGTCTGAc                                                                                                              >  1:580250/1‑131 (MQ=255)                                tCGAGATTTTCATAAGGCACACCCGTCAGTACCAGAACCTGAGTACCGCCGCCCTCTTTCTCGCCGTACAGATGCGGATAATATTTCGGCACCGTATGCAGGCAAGTGTCGCCAGATTTCAGCGTCTGACGCGGATAGTg                                                                                                    <  2:474264/140‑1 (MQ=255)                                   agaTTTTCATAAGGCACACCCGTCAGTACCAGAACCTGAGTACCGCCGCCCTCTTTCTCGCCGTACAGATGCGGATAATATTTCGGCACCGTATGCAGGCAAGTGTCGCCAGATTTCAGCGTCTGACGCGGATAGTGGTa                                                                                                 <  2:565398/140‑1 (MQ=255)                                              aGGCACACCCGTCAGTACCAGAACCTGAGTACCGCCGCCCTCTTTCTCGCCGTACAGATGCGGATAATATTTCGGCACCGTATGCAGGCAAGTGTCGCCAGATTTCAGCGTCTGACGCGGATAGTGGTATTCGCTGCCAg                                                                                      >  2:21583/1‑140 (MQ=255)                                                 cacaCCCGTCAGTACCAGAACCTGAGTACCGCCGCCCTCTTTCTCGCCGTACAGATGCGGATAATATTTCGGCACCGTATGCAGGCAAGTGTCGCCAGATTTCAGCGTCTGACGCGGATAGTGGTATTCGCTGCCAGGCt                                                                                   >  2:264106/1‑140 (MQ=255)                                                 cacaCCCGTCAGTACCAGAACCTGAGTACCGCCGCCCTCTTTCTCGCCGTACAGATGCGGATAATATTTCGGCACCGTATGCAGGCAAGTGTCGCCAGATTTCAGCGTCTGACGCGGATAGTGGTATTCGCTGCCAGGCt                                                                                   >  2:401196/1‑140 (MQ=255)                                                    acCCGTCAGTACCAGAACCTGAGTACCGCCGCCCTCTTTCTCGCCGTACAGATGCGGATAATATTTCGGCACCGTATGCAGGCAAGTGTCGCCAGATTTCAGCGTCTGACGCGGATAGTGGTATTCGCTGCCAGGCtt                                                                                  <  1:617400/138‑1 (MQ=255)                                                    acCCGTCAGTACCAGAACCTGAGTACCGCCGCCCTCTTTCTCGCCGTACAGATGCGGATAATATTTCGGCACCGTATGCAGGCAAGTGTCGCCAGATTTCAGCGTCTGACGCGGATAGTGGTATTCGCTGCCAGGCtt                                                                                  >  2:617400/1‑138 (MQ=255)                                                     cccGTCAGTACCAGAACCTGAGTACCGCCGCCCTCTTTCTCGCCGTACAGATGCGGATAATATTTCGGCACCGTATGCAGGCAAGTGTCGCCAGATTTCAGCGTCTGACGCGGATAGTGGTATTCGCTGCCAGGCTTCAg                                                                               >  1:530933/1‑140 (MQ=255)                                                            gTACCAGAACCTGAGTACCGCCGCCCTCTTTCTCGCCGTACAGATGCGGATAATATTTCGGCACCGTATGCAGGCAAGTGTCGCCAGATTTCAGCGTCTGACGCGGATAGTGGTATTCGCTGCCAGGCTTCAGCGCCAGa                                                                        >  2:244667/1‑140 (MQ=255)                                                             tACCAGAACCTGAGTACCGCCGCCCTCTTTCTCGCCGTACAGATGCGGATAATATTTCGGCACCGTATGCAGGCAAGTGTCGCCAGATTTCAGCGTCTGACGCGGATAGTGGTATTCGCTGCCAGGCTTCAGCGCCAGAc                                                                       <  2:801693/140‑1 (MQ=255)                                                                cAGAACCTGAGTACCGCCGCCCTCTTTCTCGCCGTACAGATGCGGATAATATTTCGGCACCGTATGCAGGCAAGTGTCGCCAGATTTCAGCGTCTGACGCGGATAGTGGTATTCGCTGCCAGGCTTCAGCGCCAGACGtt                                                                    >  1:1241033/1‑140 (MQ=255)                                                                  gAACCTGAGTACCGCCGCCCTCTTTCTCGCCGTACAGATGCGGATAATATTTCGGCACCGTATGCAGGCAAGTGTCGCCAGATTTCAGCGTCTGACGCGGATAGTGGTATTCGCTGCCAGGCTTCAGCGCCAGACGtttt                                                                  <  1:244667/140‑1 (MQ=255)                                                                   aaCCTGAGTACCGCCGCCCTCTTTCTCGCCGTACAGATGCGGATAATATTTCGGCACCGTATGCAGGCAAGTGTCGCCAGATTTCAGCGTCTGACGCGGATAGTGGTATTCGCTGCCAGGCTTCAGCGCCAGACGttttt                                                                 >  1:81689/1‑140 (MQ=255)                                                                     ccTGAGTACCGCCGCCCTCTTTCTCGCCGTACAGATGCGGATAATATTTCGGCACCGTATGCAGGCAAGTGTCGCCAGATTTCAGCGTCTGACGCGGATAGTGGTATTCGCTGCCAGGCTTCAGCGCCAGACGTTTTTTc                                                               >  1:203708/1‑140 (MQ=255)                                                                      cTGAGTACCGCCGCCCTCTTTCTCGCCGTACAGATGCGGATAATATTTCGGCACCGTATGCAGGCAAGTGTCGCCAGATTTCAGCGTCTGACGCGGATAGTGGTATTCGCTGCCAGGCTTCAGCGCCAGACGTTTTTTCg                                                              <  1:903449/140‑1 (MQ=255)                                                                         aGTACCGCCGCCCTCTTTCTCGCCGTACAGATGCGGATAATATTTCGGCACCGTATGCAGGCAAGTGTCGCCAGATTTCAGCGTCTGACGCGGATAGTGGTATTCGCTGCCAGGCTTCAGCGCCAGACGTTTTTTCGCCt                                                           <  1:1163614/140‑1 (MQ=255)                                                                           tACCGCCGCCCTCTTTCTCGCCGTACAGATGCGGATAATATTTCGGCACCGTATGCAGGCAAGTGTCGCCAGATTTCAGCGTCTGACGCGGATAGTGGTATTCGCTGCCAGGCTTCAGCGCCAGACGTTTTTTCGCCTcc                                                         <  1:74691/140‑1 (MQ=255)                                                                             ccgccgCCCTCTTTCTCGCCGTACAGATGCGGATAATATTTCGGCACCGTATGCAGGCAAGTGTCGCCAGATTTCAGCGTCTGACGCGGATAGTGGTATTCGCTGCCAGGCTTCAGCGCCAGACGTTTTTTCGCCTCCGc                                                       >  2:53272/1‑140 (MQ=255)                                                                                   cccTCTTTCTCGCCGTACAGATGCGGATAATATTTCGGCACCGTATGCAGGCAAGTGTCGCCAGATTTCAGCGTCTGACGCGGATAGTGGTATTCGCTGCCAGGCTTCAGCGCCAGACGTTTTTTCGCCTCCGCCATCAg                                                 <  2:1137805/140‑1 (MQ=255)                                                                                   cccTCTTTCTCGCCGTACAGATGCGGATAATATTTCGGCACCGTATGCAGGCAAGTGTCGCCAGATTTCAGCGTCTGACGCGGATAGTGGTATTCGCTGCCAGGCTTCAGCGCCAGACGTTTTTTCGCCTCCGCCATCAg                                                 <  2:771853/140‑1 (MQ=255)                                                                                             cGCCGTACAGATGCGGATAATATTTCGGCACCGTATGCAGGCAAGTGTCGCCAGATTTCAGCGTCTGACGCGGATAGTGGTATTCGCTGCCAGGCTTCAGCGCCAGACGTTTTTTCGCCTCCGCCATCAGCTCTTCAcgc                                       <  2:167092/140‑1 (MQ=255)                                                                                                cGTACAGATGCGGATAATATTTCGGCACCGTATGCAGGCAAGTGTCGCCAGATTTCAGCGTCTGACGCGGATAGTGGTATTCGCTGCCAGGCTTCAGCGCCAGACGTTTTTTCGCCTCCGCCATCAGCTCTTCACGCGTa                                    >  2:803868/1‑140 (MQ=255)                                                                                                cGTACAGATGCGGATAATATTTCGGCACCGTATGCAGGCAAGTGTCGCCAGATTTCAGCGTCTGACGCGGATAGTGGTATTCGCTGCCAGGCTTCAGCGCCAGACGTTTTTTCGCCTCCGCCATCAGCTCTTCACGCGTa                                    <  1:401196/140‑1 (MQ=255)                                                                                                          cGGATAATATTTCGGCACCGTATGCAGGCAAGTGTCGCCAGATTTCAGCGTCTGACGCGGATAGTGGTATTCGCTGCCAGGCTTCAGCGCCAGACGTTTTTTCGCCTCCGCCATCAGCTCTTCACGCGTACCGAAAATCa                          >  1:187754/1‑140 (MQ=255)                                                                                                          cGGATAATATTTCGGCACCGTATGCAGGCAAGTGTCGCCAGATTTCAGCGTCTGACGCGGATAGTGGTATTCGCTGCCAGGCTTCAGCGCCAGACGTTTTTTCGCCTCCGCCATCAGCTCTTCACGCGTACCGAAAATCa                          >  1:762211/1‑140 (MQ=255)                                                                                                            gATAATATTTCGGCACCGTATGCAGGCAAGTGTCGCCAGATTTCAGCGTCTGACGCGGATAGTGGTATTCGCTGCCAGGCTTCAGCGCCAGACGTTTTTTCGCCTCCGCCATCAGCTCTTCACGCGTACCGAAAATCAcc                        >  2:758711/1‑140 (MQ=255)                                                                                                            gATAATATTTCGGCACCGTATGCAGGCAAGTGTCGCCAGATTTCAGCGTCTGACGCGGATAGTGGTATTCGCTGCCAGGCTTCAGCGCCAGACGTTTTTTCGCCTCCGCCATCAGCTCTTCACGCGTACCGAAAATCAcc                        >  2:425381/1‑140 (MQ=255)                                                                                                                   tttCGGCACCGTATGCAGGCAAGTGTCGCCAGATTTCAGCGTCTGACGCGGATAGTGGTATTCGCTGCCAGGCTTCAGCGCCAGACGTTTTTTCGCCTCCGCCATCAGCTCTTCACGCGTACCGAAAATCACCGcgcccg                 <  1:489549/140‑1 (MQ=255)                                                                                                                     tCGGCACCGTATGCAGGCAAGTGTCGCCAGATTTCAGCGTCTGACGCGGATAGTGGTATTCGCTGCCAGGCTTCAGCGCCAGACGTTTTTTCGCCTCCGCCATCAGCTCTTCACGCGTACCGAAAATCACCGcgcccgcc               <  2:1344042/140‑1 (MQ=255)                                                                                                                     tCGGCACCGTATGCAGGCAAGTGTCGCCAGATTTCAGCGTCTGACGCGGATAGTGGTATTCGCTGCCAGGCTTCAGCGCCAGACGTTTTTTCGCCTCCGCCATCAGCTATTCTCGCGTGCCGAAAATCACCGcgcccgcc               <  2:348940/140‑1 (MQ=255)                                                                                                                       ggCACCGTATGCAGGCAAGTGTCGCCAGATTTCAGCGTCTGACGCGGATAGTGGTATTCGCTGCCAGGCTTCAGCGCCAGACGTTTTTTCGCCTCCGCCATCAGCTCTTCACGCGTACCGAAAATCACCGCGCCCGCCgg             >  2:733621/1‑140 (MQ=255)                                                                                                                       ggCACCGTATGCAGGCAAGTGTCGCCAGATTTCAGCGTCTGACGCGGATAGTGGTATTCGCTGCCAGGCTTCAGCGCCAGACGTTTTTTCGCCTCCGCCATCAGCTCTTCACGCGTACCGAAAATCACCGCGCCCGCCgg             >  1:1230719/1‑140 (MQ=255)                                                                                                                         cACCGTATGCAGGCAAGTGTCGCCAGATTTCAGCGTCTGACGCGGATAGTGGTATTCGCTGCCAGGCTTCAGCGCCAGACGTTTTTTCGCCTCCGCCATCAGCTCTTCACGCGTACCGAAAATCACCGCGCCCGCCGGGc           >  2:1282090/1‑140 (MQ=255)                                                                                                                          aCCGTATGCAGGCAAGTGTCGCCAGATTTCAGCGTCTGACGCGGATAGTGGTATTCGCTGCCAGGCTTCAGCGCCAGACGTTTTTTCGCCTCCGCCATCAGCTCTTCACGCGTACCGAAAATCACCGCGCCCGCCGGGca          <  2:1259220/140‑1 (MQ=255)                                                                                                                          aCCGTATGCAGGCAAGTGTCGCCAGATTTCAGCGTCTGACGCGGATAGTGGTATTCGCTGCCAGGCTTCAGCGCCAGACGTTTTTTCGCCTCCGCCATCAGCTCTTCACGCGTACCGAAAATCACCGCGCCCGCCGGGca          <  1:21583/140‑1 (MQ=255)                                                                                                                          aCCGTATGCAGGCAAGTGTCGCCAGATTTCAGCGTCTGACGCGGATAGTGGTATTCGCTGCCAGGCTTCAGCGCCAGACGTTTTTTCGCCTCCGCCATCAGCTCTTCACGCGTACCGAAAATCACCGCGCCCGCCGGGca          <  1:1282090/140‑1 (MQ=255)                                                                                                                            cGTATGCAGGCAAGTGTCGCCAGATTTCAGCGTCTGACGCGGATAGTGGTATTCGCTGCCAGGCTTCAGCGCCAGACGTTTTTTCGCCTCCGCCATCAGCTCTTCACGCGTACCGAAAATCACCGCGCCCGCCGGGcaca        >  2:164104/1‑140 (MQ=255)                                                                                                                            cGTATGCAGGCAAGTGTCGCCAGATTTCAGCGTCTGACGCGGATAGTGGTATTCGCTGCCAGGCTTCAGCGCCAGACGTTTTTTCGCCTCCGCCATCAGCTCTTCACGCGTACCGAAAATCACCGCGCCCGCCGGGcaca        >  2:1349674/1‑140 (MQ=255)                                                                                                                              tATGCAGGCAAGTGTCGCCAGATTTCAGCGTCTGACGCGGATAGTGGTATTCGCTGCCAGGCTTCAGCGCCAGACGTTTTTTCGCCTCCGCCATCAGCTCTTCACGCGTACCGAAAATCACCGCGCCCGCCGGGCACACt      >  2:184701/1‑140 (MQ=255)                                                                                                                              tATGCAGGCAAGTGTCGCCAGATTTCAGCGTCTGACGCGGATAGTGGTATTCGCTGCCAGGCTTCAGCGCCAGACGTTTTTTCGCCTCCGCCATCAGCTCTTCACGCGTACCGAAAATCACCGCGCCCGCCGGGCACACt      >  2:1163402/1‑140 (MQ=255)                                                                                                                              tATGCAGGCAAGTGTCGCCAGATTTCAGCGTCTGACGCGGATAGTGGTATTCGCTGCCAGGCTTCAGCGCCAGACGTTTTTTCGCCTCCGCCATCAGCTCTTCACGCGTACCGAAAATCACCGCGCCCGCCGGGCACACt      >  1:947230/1‑140 (MQ=255)                                                                                                                              tATGCAGGCAAGTGTCGCCAGATTTCAGCGTCTGACGCGGATAGTGGTATTCGCTGCCAGGCTTCAGCGCCAGACGTTTTTTCGCCTCCGCCATCAGCTCTTCACGCGTACCGAAAATCACCGCGCCCGCCGGGCACACt      >  1:296333/1‑140 (MQ=255)                                                                                                                              tATGCAGGCAAGTGTCGCCAGATTTCAGCGTCTGACGCGGATAGTGGTATTCGCTGCCAGGCTTCAGCGCCAGACGTTTTTTCGCCTCCGCCATCAGCTCTTCACGCGTACCGAAAATCACCGCGCCCGCCGGGCACACt      >  1:1360814/1‑140 (MQ=255)                                                                                                                                  cAGGCAAGTGTCGCCAGATTTCAGCGTCTGACGCGGATAGTGGTATTCGCTGCCAGGCTTCAGCGCCAGACGTTTTTTCGCCTCCGCCATCAGCTCTTCACGCGTACCGAAAATCACCGCGCCCGCCGGGCACACTTCTa  <  2:530933/140‑1 (MQ=255)                                                                                                                                  cAGGCAAGTGTCGCCAGATTTCAGCGTCTGACGCGGATAGTGGTATTCGCTGCCAGGCTTCAGCGCCAGACGTTTTTTCGCCTCCGCCATCAGCTCTTCACGCGTACCGAAAATCACCGCGCCCGCCGGGCACACTTCTa  <  2:1241033/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                        ACCGGTAGAAAGATCGTCCAGTTTCGGCAGGTCGAGATTTTCATAAGGCACACCCGTCAGTACCAGAACCTGAGTACCGCCGCCCTCTTTCTCGCCGTACAGATGCGGATAATATTTCGGCACCGTATGCAGGTAAGTGTCGCCAGATTTCAGCGTCTGACGCGGATAGTGGTATTCGCTGCCAGGCTTCAGCGCCAGACGTTTTTTCGCCTCCGCCATCAGCTCTTCACGCGTACCGAAAATCACCGCGCCCGCCGGGCACACTTCTA  >  NC_000913/3144292‑3144560

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑