Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,208,044	G→A	W7* (TGG→TGA)	ttdT →	L‑tartrate:succinate antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,208,044	0	G	A	100.0%	202.5 / NA	58	W7* (TGG→TGA)	ttdT	L‑tartrate:succinate antiporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/29);  total (29/29)

TTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGCTCACCCCTCTCCTTTTTCGCAGGCATAACACGATGAAACCTTCCACTGAATGGTGGCGATATCTTGCGCCGCTGGCGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGC  >  NC_000913/3207914‑3208180                                                                                                                                   |                                                                                                                                         ttCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGCTCACCCCTCTCCTTTTTCGCAGGCATAACACGATGAAACCTTCCACTGAATGATGGCGatat                                                                                                                                 >  2:536038/1‑140 (MQ=255)             cgcgATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGCTCACCCCTCTCCTTTTTCGCAGGCATAACACGATGAAACCTTCCACTGAATGATGGCGATATCTTGCGCCGCTg                                                                                                                     >  2:468913/1‑140 (MQ=255)                   cccATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGCTCACCCCTCTCCTTTTTCGCAGGCATAACACGATGAAACCTTCCACTGAATGATGGCGATATCTTGCGCCGCTGGCGGTc                                                                                                               <  2:1062176/140‑1 (MQ=255)                     cATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGCTCACCCCTCTCCTTTTTCGCAGGCATAACACGATGAAACCTTCCACTGAATGATGGCGATATCTTGCGCCGCTGGCGGTCAt                                                                                                             <  2:942854/140‑1 (MQ=255)                     cATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGCTCACCCCTCTCCTTTTTCGCAGGCATAACACGATGAAACCTTCCACTGAATGATGGCGATATCTTGCGCCGCTGGCGGTCAt                                                                                                             <  2:667845/140‑1 (MQ=255)                     cATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGCTCACCCCTCTCCTTTTTCGCAGGCATAACACGATGAAACCTTCCACTGAATGATGGCGATATCTTGCGCCGCTGGCGGTCAt                                                                                                             >  1:116103/1‑140 (MQ=255)                        cGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGCTCACCCCTCTCCTTTTTCGCAGGCATAACACGATGAAACCTTCCACTGAATGATGGCGATATCTTGCGCCGCTGGCGGTCATCGc                                                                                                          <  1:536038/140‑1 (MQ=255)                           ggAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGCTCACCCCTCTCCTTTTTCGCAGGCATAACACGATGAAACCTTCCACTGAATGATGGCGATATCTTGCGCCGCTGGCGGTCATCGCCat                                                                                                       <  1:1376368/140‑1 (MQ=255)                            gAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGCTCACCCCTCTCCTTTTTCGCAGGCATAACACGATGAAACCTTCCACTGAATGATGGCGATATCTTGCGCCGCTGGCGGTCATCGc                                                                                                          >  2:1370840/1‑136 (MQ=255)                            gAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGCTCACCCCTCTCCTTTTTCGCAGGCATAACACGATGAAACCTTCCACTGAATGATGGCGATATCTTGCGCCGCTGGCGGTCATCGc                                                                                                          <  1:1370840/136‑1 (MQ=255)                            gAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGCTCACCCCTCTCCTTTTTCGCAGGCATAACACGATGAAACCTTCCACTGAATGATGGCGATATCTTGCGCCGCTGGCGGTCATCGCCatt                                                                                                      >  2:1300403/1‑140 (MQ=255)                              agagATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGCTCACCCCTCTCCTTTTTCGCAGGCATAACACGATGAAACCTTCCACTGAATGATGGCGATATCTTGCGCCGCTGGCGGTCATCGCCattat                                                                                                    <  2:116103/140‑1 (MQ=255)                                 gaTCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGCTCACCCCTCTCCTTTTTCGCAGGCATAACACGATGAAACCTTCCACTGAATGATGGCGATATCTTGCGCCGCTGGCGGTCATCGCCATTATTGc                                                                                                 <  1:167868/140‑1 (MQ=255)                                  aTCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGCTCACCCCTCTCCTTTTTCGCAGGCATAACACGATGAAACCTTCCACTGAATGATGGCGATATCTTGCGCCGCTGGCGGTCATCGCCATTATTGct                                                                                                <  1:1070337/140‑1 (MQ=255)                                  aTCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGCTCACCCCTCTCCTTTTTCGCAGGCATAACACGATGAAACCTTCCACTGAATGATGGCGATATCTTGCGCCGCTGGCGGTCATCGCCATTATTGct                                                                                                >  2:353578/1‑140 (MQ=255)                                  aTCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGCTCACCCCTCTCCTTTTTCGCAGGCATAACACGATGAAACCTTCCACTGAATGATGGCGATATCTTGCGCCGCTGGCGGTCATCGCCATTATTGct                                                                                                <  1:741174/140‑1 (MQ=255)                                       cGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGCTCACCCCTCTCCTTTTTCGCAGGCATAACACGATGAAACCTTCCACTGAATGATGGCGATATCTTGCGCCGCTGGCGGTCATCGCCATTATTGCTCTACt                                                                                           <  1:1388276/140‑1 (MQ=255)                                        gaccACGTCAATTACATCAAATAACCCCCCCGGAGAGGCTCACCCCTCTCCTTTTTCGCAGGCATAACACGATGAACCCTTCCACTGAATGATGGCGATATCTTGCGCCGCGGGCGGTCATCGCCATTATTGCTCTACtc                                                                                          <  2:175562/137‑2 (MQ=255)                                           cACGTCCATTACATCAAATAACCCTCCCGGAGAGGCTCACCCCTCTCCTTTTTCGCAGGCATAACACGATGAAACCTTCCACTGAATGATGGCGATATCTTGCGCCGCTGGCGGTCATCGCCATTATTGCTCTACTTCCg                                                                                       >  1:897992/1‑140 (MQ=255)                                             cGTCCATTACATCAAATAACCCTCCCGGAGAGGCTCACCCCTCTCCTTTTTCGCAGGCATAACACGATGAAACCTTCCACTGAATGATGGCGATATCTTGCGCCGCTGGCGGTCATCGCCATTATTGCTCTACTTCCGGt                                                                                     <  1:809482/140‑1 (MQ=255)                                                        tCAAATAACCCTCCCGGAGAGGCTCACCCCTCTCCTTTTTCGCAGGCATAACACGATGAAACCTTCCACTGAATGATGGCGATATCTTGCGCCGCTGGCGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGc                                                                          >  2:170749/1‑140 (MQ=255)                                                          aaaTAACCCTCCCGGAGAGGCTCACCCCTCTCCTTTTTCGCAGGCATAACACGATGAAACCTTCCACTGAATGATGGCGATATCTTGCGCCGCTGGCGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTg                                                                        <  2:897992/140‑1 (MQ=255)                                                          aaaTAACCCTCCCGGAGAGGCTCACCCCTCTCCTTTTTCGCAGGCATAACACGATGAAACCTTCCACTGAATGATAGCGATATCTTGCGCCGCTGGCGGGCAGCGCCCTTTTTACCCTACTCCCGGGTCCCGCCGGGCTg                                                                        >  2:266321/1‑140 (MQ=255)                                                           aaTAACCCTCCCGGAGAGGCTCCCCCCTCTCCTTTTTCGCAGGCATAACACGATGAAACCTTCCACTGAATGATGGCGATATCTTGCGCCGCTGGCGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTgg                                                                       <  2:172207/140‑1 (MQ=255)                                                           aaTAACCCTCCCGGAGAGGCTCACCCCTCTCCTTTTTCGCAGGCATAACACGATGAAACCTTCCACTGAATGATGGCGATATCTTGCGCCGCTGGCGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTgg                                                                       <  2:469635/140‑1 (MQ=255)                                                                cccTCCCGGAGAGGCTCACCCCTCTCCTTTTTCGCAGGCATAACACGATGAAACCTTCCACTGAATGATGGCGATATCTTGCGCCGCTGGCGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAAc                                                                  >  1:1234792/1‑140 (MQ=255)                                                                cccTCCCGGAGAGGCTCACCCCTCTCCTTTTTCGCAGGCATAACACGATGAAACCTTCCACTGAATGATGGCGATATCTTGCGCCGCTGGCGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAAc                                                                  <  2:1396322/140‑1 (MQ=255)                                                                    ctccgggagGCTCACCCCTCTCCTTTTTCGCAGGCATAACACGATGAAACCTTCCACTGAATGATGGCGATATCTTGCGCCGCTGGCGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCa                                                                <  1:1117469/132‑1 (MQ=255)                                                                    ctccgggagGCTCACCCCTCTCCTTTTTCGCAGGCATAACACGATGAAACCTTCCACTGAATGATGGCGATATCTTGCGCCGCTGGCGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCa                                                                >  2:1117469/7‑138 (MQ=255)                                                                    cccGGAGAGGCTCACCCCTCTCCTTTTTCGCAGGCATAACACGATGAAACCTTCCACTGAATGATGGCGATATCTTGCGCCGCTGGCGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATa                                                              <  2:69986/140‑1 (MQ=255)                                                                     ccGGAGAGGCTCACCCCTCTCCTTTTTCGCAGGCATAACACGATGAAACCTTCCACTGAATGATGGCGATATCTTGCGCCGCTGGCGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGaa                                                                   <  1:823890/134‑1 (MQ=255)                                                                     ccGGAGAGGCTCACCCCTCTCCTTTTTCGCAGGCATAACACGATGAAACCTTCCACTGAATGATGGCGATATCTTGCGCCGCTGGCGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGaa                                                                   >  2:823890/1‑134 (MQ=255)                                                                     ccGGAGAGGCTCACCCCTCTCCTTTTTCGCAGGCATAACACGATGAAACCTTCCACTGAATGATGGCGATATCTTGCGCCGCTGGCGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATAc                                                             >  1:342169/1‑140 (MQ=255)                                                                     ccGGAGAGGCTCACCCCTCTCCTTTTTCGCAGGCATAACACGATGAAACCTTCCACTGAATGATGGCGATATCTTGCGCCGCTGGCGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATAc                                                             >  1:1246565/1‑140 (MQ=255)                                                                     ccGGAGAGGCTCACCCCTCTCCTTTTTCGCAGGCATAACACGATGAAACCTTCCACTGAATGATGGCGATATCTTGCGCCGCTGGCGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATAc                                                             >  2:1068174/1‑140 (MQ=255)                                                                      cGGAGAGGCTCACCCCTCTCCTTTTTCGCAGGCATAACACGATGAAACCTTCCACTGAATGATGGCGATATCTTGCGCCGCTGGCGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATAcc                                                            <  2:1246565/140‑1 (MQ=255)                                                                             gCTCACCCCTCTCCTTTTTCGCAGGCATAACACGATGAAACCTTCCACTGAATGATGGCGATATCTTGCGCCGCTGGCGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGctct                                                     >  2:873338/1‑140 (MQ=255)                                                                              cTCACCCCTCTCCTTTTTCGCAGGCATAACACGATGAAACCTTCCACTGAATGATGGCGATATCTTGCGCCGCTGGCGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTa                                                    >  2:1107928/1‑140 (MQ=255)                                                                               tCACCCCTCTCCTTTTTCGCAGGCATAACACGATGAAACCTTCCACTGAATGATGGCGATATCTTGCGCCGCTGGCGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTAc                                                   >  1:1165619/1‑140 (MQ=255)                                                                                       ctcCTTTTTCGCAGGCATAACACGATGAAACCTTCCACTGAATGATGGCGATATCTTGCGCCGCTGGCGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGt                                           >  2:106001/1‑140 (MQ=255)                                                                                       ctcCTTTTTCGCAGGCATAACACGATGAAACCTTCCACTGAATGATGGCGATATCTTGCGCCGCTGGCGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGt                                           >  2:593399/1‑140 (MQ=255)                                                                                            ttttCGCAGGCATAACACGATGAAACCTTCCACTGAATGATGGCGATATCTTGCGCCGCTGGCGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTa                                      <  2:724740/140‑1 (MQ=255)                                                                                             tttCGCAGGCATAACACGATGAAACCTTCCACTGAATGATGGCGATATCTTGCGCCGCTGGCGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTAc                                     >  1:422256/1‑140 (MQ=255)                                                                                             tttCGCAGGCATAACACGATGAAACCTTCCACTGAATGATGGCGATATCTTGCGCCGCTGGCGGCCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTAc                                     >  2:573706/1‑140 (MQ=255)                                                                                               tCGCAGGCATAACACGATGAAACCTTCCACTGAATGATGGCGATATCTTGCGCCGCTGGCGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTg                                   >  2:504209/1‑140 (MQ=255)                                                                                                   aGGCATAACACGATGAAACCTTCCACTGAATGATGGCGATATCTTGCGCCGCTGGCGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGt                               <  1:1300403/140‑1 (MQ=255)                                                                                                   aGGCATAACACGATGAAACCTTCCACTGAATGATGGCGATATCTTGCGCCGCTGGCGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGt                               <  2:353878/140‑1 (MQ=255)                                                                                                   aGGCATAACACGATGAAACCTTCCACTGAATGATGGCGATATCTTGCGCCGCTGGCGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGt                               <  1:1107928/140‑1 (MQ=255)                                                                                                    ggCATAACACGATGAAACCTTCCACTGAATGATGGCGATATCTTGCGCCGCTGGCGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTg                              >  1:976846/1‑140 (MQ=255)                                                                                                        gaaCACGATGAAACCTTCCACTGAATGATGGCGATATCTTGCGCCGCTGGCGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCg                          <  1:873338/139‑1 (MQ=255)                                                                                                              gATGAAACCTTCCACTGAATGATGGCGATATCTTGCGCCGCTGGCGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGAc                    <  1:106001/140‑1 (MQ=255)                                                                                                               aTGAAACCTTCCACTGAATGATGGCGATATCTTGCGCCGCTGGCGGTCATCGCCATTATTGGTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACt                   >  2:1249850/1‑140 (MQ=255)                                                                                                               aTGAAACCTTCCACTGAATGATGGCGATATCTTGCGCCGCTGGCGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACt                   >  2:713145/1‑140 (MQ=255)                                                                                                                tGAAACCTTCCACTGAATGATGGCGATATCTTGCGCCGCTGGCGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTg                  >  1:743088/1‑140 (MQ=255)                                                                                                                tGAAACCTTCCACTGAATGATGGCGATATCTTGCGCCGCTGGCGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTg                  <  2:1165619/140‑1 (MQ=255)                                                                                                                   aaCCTTCCACTGAATGATGGCGATATCTTGCGCCGCTGGCGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATc               >  2:455595/1‑140 (MQ=255)                                                                                                                   aaCCTTCCACTGAATGATGGCGATATCTTGCGCCGCTGGCGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATc               >  1:661019/1‑140 (MQ=255)                                                                                                                          cACTGAATGATGGCGATATCTTGCGCCGCTGGCGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAAc        <  1:713145/140‑1 (MQ=255)                                                                                                                               aaTGATGGCGATATCTTGCGCCGCTGGCGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTg   >  2:1036853/1‑140 (MQ=255)                                                                                                                               aaTGATGGCGATATCTTGCGCCGCTGGCGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTg   <  1:170749/140‑1 (MQ=255)                                                                                                                                atgatgGCGATATCTTGCGCCGCTGGCGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGc  <  1:243825/140‑1 (MQ=255)                                                                                                                                atgatgGCGATATCTTGCGCCGCTGGCGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGc  <  2:422256/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                         TTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGCTCACCCCTCTCCTTTTTCGCAGGCATAACACGATGAAACCTTCCACTGAATGGTGGCGATATCTTGCGCCGCTGGCGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGC  >  NC_000913/3207914‑3208180

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑