Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,209,542	T→C	L334L (TTA→TTG)	tsaD ←	N(6)‑L‑threonylcarbamoyladenine synthase, TsaD subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,209,542	0	T	C	100.0%	334.3 / NA	91	L334L (TTA→TTG)	tsaD	N(6)‑L‑threonylcarbamoyladenine synthase, TsaD subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (48/43);  total (48/43)

GACTGGGGGCGATTTTTGGGCTGATATTCCTCGTATTGCTGGTGATTACCGGCTTACTGTGGATGCCCGTGGTGTTGCTTTAAGTGAGGACGGTGCCGGAAATTGCTCTCCGGCACCCAATAGTTTTACGCAGCCGGTAACTCCGCCAGCGGCCAGCGCGGACGCACGCTAACGCCGAGATCCGCCGTCGCGCCTGCTTTAAACCGCACCATTCCGGCATAGGCGATCATCGCGCCGTTATCAGTACAAAATTCCGGACGCGCG  >  NC_000913/3209405‑3209668                                                                                                                                          |                                                                                                                               gACTGGGGGCGATTTTTGGGCTGATATTCCTCGTATTGCTGGTGATTACCGGCTTACTGTGGATGCCCGTGGTGTTGCTTTAAGTGAGGACGGTGCCGGAAATTGCTCTCCGGCACCCAATAGTTTTACGCAGCCGGCaa                                                                                                                              >  2:1001112/1‑140 (MQ=255) gACTGGGGGCGATTTTTGGGCTGATATTCCTCGTATTGCTGGTGATTACCGGCTTACTGTGGATGCCCGTGGTGTTGCTTTAAGTGAGGACGGTGCCGGAAATTGCTCTCCGGCACCCAATAGTTTTACGCAGCCGGCaa                                                                                                                              >  1:1380154/1‑140 (MQ=255)  aCTGGGGGCGATTTTTGGGCTGATATTCCTCGTATTGCTGGTGATTACCGGCTTACTGTGGATGCCCGTGGTGTTGCTTTAAGTGAGGACGGTGCCGGAAATTGCTCTCCGGCACCCAATAGTTTTACGCAGCCGGCAAc                                                                                                                             >  2:218468/1‑140 (MQ=255)    tGGGGGCGATTTTTGGGCTGATATTCCTCGTATTGCTGGTGATTACCGGCTTACTGTGGATGCCCGTGGTGTTGCTTTAAGTGAGGACGGTGCCGGAAATTGCTCTCCGGCACCCAATAGTTTTACGCAGCCGGCAACTc                                                                                                                           >  1:679135/1‑140 (MQ=255)      ggggCGATTTTTGGGCTGATATTCCTCGTATTGCTGGTGATTACCGGCTTACTGTGGATGCCCGTGGTGTTGCTTTAAGTGAGGACGGTGCCGGAAATTGCTCTCCGGCACCCAATAGTTTTACGCAGCCGGCAACTCCg                                                                                                                         >  1:898932/1‑140 (MQ=255)         gCGATTTTTGGGCTGATATTCCTCGTATTGCTGGTGATTACCGGCTTACTGTGGATGCCCGTGGTGTTGCTTTAAGTGAGGACGGTGCCGGAAATTGCTCTCCGGCACCCAATAGTTTTACGCAGCCGGCAACTCCgcca                                                                                                                      >  1:905870/1‑140 (MQ=255)                  gggCTGATATTCCTCGTATTGCTGGTGATTACCGGCTTACTGTGGATGCCCGTGGTGTTGCTTTAAGTGAGGACGGTGCCGGAAATTGCTCTCCGGCA‑CCAATAGTTTTACGCAGCCGGCAACTCCgccagcggccagcg                                                                                                            >  2:1257155/1‑140 (MQ=255)                   ggCTGATATTCCTCGTATTGCTGGTGATTACCGGCTTACTGTGGATGCCCGTGGTGTTGCTTTAAGTGAGGACGGTGCCGGAAATTGCTCTCCGGCACCCAATAGTTTTACGCAGCCGGCAACTCCgccagcggccagcg                                                                                                            <  2:679135/140‑1 (MQ=255)                        atatTCCTCGTATTGCTGGTGATTACCGGCTTACTGTGGATGCCCGTGGTGTTGCTTTAAGTGAGGACGGTGCCGGAAATTGCTCTCCGGCACCCAATAGTTTTACGCAGCCGGCAACTCCGCCAGCGGCCAGCGCGGac                                                                                                       >  1:461342/1‑140 (MQ=255)                                 gTATTGCTGGTGATTACCGGCTTACTGTGGATGCCCGTGGTGTTGCTTTAAGTGAGGACGGTGCCGGAAATTGCTCTCCGGCACCCAATAGTTTTACGCAGCCGGCAACTCCGCCAGCGGCCAGCGCGGACGCACGCTaa                                                                                              <  1:611334/140‑1 (MQ=255)                                  tATTGCTGGTGATTACCGGCTTACTGTGGATGCCCGTGGTGTTGCTTTAAGTGAGGACGGTGCCGGAAATTGCTCTCCGGCACCCAATAGTTTTACGCAGCCGGCAACTCCgccagcggccagcg                                                                                                            <  2:1308430/125‑1 (MQ=255)                                  tATTGCTGGTGATTACCGGCTTACTGTGGATGCCCGTGGTGTTGCTTTAAGTGAGGACGGTGCCGGAAATTGCTCTCCGGCACCCAATAGTTTTACGCAGCCGGCAACTCCgccagcggccagcg                                                                                                            >  1:1308430/1‑125 (MQ=255)                                  tATTGCTGGTGATTACCGGCTTACTGTGGATGCCCGTGGTGTTGCTTTAAGTGAGGACGGTGCCGGAAATTGCTCTCCGGCACCCAATAGTTTTACGCAGCCGGCAACTCCGCCAGCGGCCAGCGCGGacgca                                                                                                    >  2:441901/1‑133 (MQ=255)                                  tATTGCTGGTGATTACCGGCTTACTGTGGATGCCCGTGGTGTTGCTTTAAGTGAGGACGGTGCCGGAAATTGCTCTCCGGCACCCAATAGTTTTACGCAGCCGGCAACTCCGCCAGCGGCCAGCGCGGacgca                                                                                                    <  1:441901/133‑1 (MQ=255)                                  tATTGCTGGTGATTACCGGCTTACTGTGGATGCCCGTGGTGTTGCTTTAAGTGAGGACGGTGCCGGAAATTGCTCTCCGGCACCCAATAGTTTTACGCAGCCGGCAACTCCGCCAGCGGCCAGCGCGGACGCACGCTAAc                                                                                             >  2:996691/1‑140 (MQ=255)                                  tATTGCTGGTGATTACCGGCTTACTGTGGATGCCCGTGGTGTTGCTTTAAGTGAGGACGGTGCCGGAAATTGCTCTCCGGCACCCAATAGTTTTACGCAGCCGGCAACTCCGCCAGCGGCCAGCGCGGACGCACGCTAAc                                                                                             >  1:1238199/1‑140 (MQ=255)                                  tATTGCTGGTGATTACCGGCTTACTGTGGATGCCCGTGGTGTTGCTTTAAGTGAGGACGGTGCCGGAAATTGCTCTCCGGCACCCAATAGTTTTACGCAGCCGGCAACTCCGCCAGCGGCCAGCGCGGACACACGCTAAc                                                                                             >  1:343014/1‑140 (MQ=255)                                         ggTGATTACCGGCTTACTGTGGATGCCCGTGGTGTTGCTTTAAGTGAGGACGGTGCCGGAAATTGCTCTCCGGCACCCAATAGTTTTACGCAGCCGGCAACTCCGCCAGCGGCCAGCGCGGACGCACGCTAACGCCgaga                                                                                      <  2:1238199/140‑1 (MQ=255)                                          gTGATTACCGGCTTACTGTGGATGCCCGTGGTGTTGCTTTAAGTGAGGACGGTGCCGGAAATTGCTCTCCGGCACCCAATAGTTTTACGCAGCCGGCAACTCCGCCAGCGGCCAGCGCGGACGCACGCTAACGCCGAGAt                                                                                     >  1:997827/1‑140 (MQ=255)                                          gTGATTACCGGCTTACTGTGGATGCCCGTGGTGTTGCTTTAAGTGAGGACGGTGCCGGAAATTGCTCTCCGGCACCCAATAGTTTTACGCAGCCGGCAACTCCGCCAGCGGCCAGCGCGGACGCACGCTAACGCCGAGAt                                                                                     >  1:150958/1‑140 (MQ=255)                                            gATTACCGGCTTACTGTGGATGCCCGTGGTGTTGCTTTAAGTGAGGACGGTGCCGGAAATTGCTCTCCGGCACCCAATAGTTTTACGCAGCCGGCAACTCCGCCAGCGGCCAGCGCGGACGCACGCTAACGCCGAGATcc                                                                                   <  2:858342/140‑1 (MQ=255)                                             aTTACCGGCTTACTGTGGATGCCCGTGGTGTTGCTTTAAGTGAGGACGGTGCCGGAAATTGCTCTCCGGCACCCAATAGTTTTACGCAGCCGGCAACTCCGCCAGCGGCCAGCGCGGACGCACGCTAACGCCGAGATccg                                                                                  >  2:712873/1‑140 (MQ=255)                                             aTTACCGGCTTACTGTGGATGCCCGTGGTGTTGCTTTAAGTGAGGACGGTGCCGGAAATTGCTCTCCGGCACCCAATAGTTTTACGCAGCCGGCAACTCCGCCAGCGGCCAGCGCGGACGCACGCTAACGCCGAGATccg                                                                                  >  1:1119005/1‑140 (MQ=255)                                               tACCGGCTTACTGTGGATGCCCGTGGTGTTGCTTTAAGTGAGGACGGTGCCGGAAATTGCTCTCCGGCACCCAATAGTTTTACGCAGCCGGCAACTCCGCCAGCGGCCAGCGCGGACGCACGCTAACGCCGAGATccgcc                                                                                >  1:613436/1‑140 (MQ=255)                                               tACCGGCTTACTGTGGATGCCCGTGGTGTTGCTTTAAGTGAGGACGGTGCCGGAAATTGCTCTCCGGCACCCAATAGTTTTACGCAGCCGGCAACTCCGCCAGCGGCCAGCGCGGACGCACGCTAACGCCGAGATccgcc                                                                                >  1:1335814/1‑140 (MQ=255)                                                      ttACTGTGGATGCCCGTGGTGTTGCTTTAAGTGAGGACGGTGCCGGAAATTGCTCTCCGGCACCCAATAGTTTTACGCAGCCGGCAACTCCGCCAGCGGCCAGCGCGGACGCACGCTAACGCCGAGATCCGCCGTCgcgc                                                                         <  2:681574/140‑1 (MQ=255)                                                      ttACTGTGGATGCCCGTGGTGTTGCTTTAAGTGAGGACGGTGCCGGAAATTGCTCTCCGGCACCCAATAGTTTTACGCAGCCGGCAACTCCGCCAGCGGCCAGCGCGGACGCACGCTAACGCCGAGATCCGCCGTCgcgc                                                                         <  2:613436/140‑1 (MQ=255)                                                      ttACTGTGGATGCCCGTGGTGTTGCTTTAAGTGAGGACGGTGCCGGAAATTGCTCTCCGGCACCCAATAGTTTTACGCAGCCGGCAACTCCGCCAGCGGCCAGCGCGGACGCACGCTAACGCCGAGATCCGCCGTCgcgc                                                                         <  1:1001112/140‑1 (MQ=255)                                                      ttACTGTGGATGCCCGTGGTGTTGCTTTAAGTGAGGACGGTGCCGGAAATTGCTCTCCGGCACCCAATAGTTTTACGCAGCCGGCAACTCCGCCAGCGGCCAGCGCGGACACACGCTAACGCCGAGATCCGCCGTCgcgc                                                                         <  2:343014/140‑1 (MQ=255)                                                      ttACTGTGGATGCCCGTGGTGTTGCTTTAAGTGAGGACGGTGCCGGAAATTGCTCTCCGGCACCCAATAGTTTCACGCAGCCGGCAACTCCGCCAGCGGCCAGCGCGGACGCACGCTAACGCCGAGATCCGCCGTCgcgc                                                                         <  1:505760/140‑1 (MQ=255)                                                            tgGATGCCCGTGGTGTTGCTTTAAGTGAGGACGGTGCCGGAAATTGCTCTCCGGCACCCAATAGTTTTACGCAGCCGGCAACTCCGCCAGCGGCCAGCGCGGACGCACGCTAACGCCGAGATCCGCCGACGCGCCTGCtt                                                                   <  1:1255989/140‑1 (MQ=255)                                                              gATGCCCGTGGTGTTGCTTTAAGTGAGGACGGTGCCGGAAATTGCTCTCCGGCACCCAATAGTTTTACGCAGCCGGCAACTCCGCCAGCGGCCAGCGCGGACGCACGCTAACGCCGAGATCCGCCGTCGCGCCTGCTTTa                                                                 >  1:35053/1‑140 (MQ=255)                                                              gATGCCCGTGGTGTTGCTTTAAGTGAGGACGGTGCCGGAAATTGCTCTCCGGCACCCAATAGTTTTACGCAGCCGGCAACTCCGCCAGCGGCCAGCGCGGACGCACGCTAACGCCGAGATCCGCCGTCGCGCCTGCTTTa                                                                 >  2:371761/1‑140 (MQ=255)                                                              gATGCCCGTGGTGTTGCTTTAAGTGAGGACGGTGCCGGAAATTGCTCTCCGGCACCCAATAGTTTTACGCAGCCGGCAACTCCGCCAGCGGCCAGCGCGGACGCACGCTAACGCCGAGATCCGCCGTCGCGCCTGCTTTa                                                                 >  1:301474/1‑140 (MQ=255)                                                               aTGCCCGTGGTGTTGCTTTAAGTGAGGACGGTGCCGGAAATTGCTCTCCGGCACCCAATAGTTTTACGCAGCCGGCAACTCCGCCAGCGGCCAGCGCGGACGCACGCTAACGCCGAGATCCGCCGTCGCGCCTGCTTTa                                                                 >  2:272039/1‑139 (MQ=255)                                                               aTGCCCGTGGTGTTGCTTTAAGTGAGGACGGTGCCGGAAATTGCTCTCCGGCACCCAATAGTTTTACGCAGCCGGCAACTCCGCCAGCGGCCAGCGCGGACGCACGCTAACGCCGAGATCCGCCGTCGCGCCTGCTTTa                                                                 <  1:272039/139‑1 (MQ=255)                                                               aTGCCCGTGGAGTTGCTTTAAGTGAGGACGGTGCCGGAAATTGCTCTCCGGCACCCAATAGTTTTACGCAGCCGGCAACTCCGCCAGCGGCCAGCGCGGACGCACGCTAACGCCGAGATCCGCCGTCGCGCCTGCTTTaa                                                                >  1:1330882/1‑140 (MQ=255)                                                                tGCCCGTGGTGTTGCTTTAAGTGAGGACGGTGCCGGAAATTGCTCTCCGGCACCCAATAGTTTTACGCAGCCGGCAACTCCGCCAGCGGCCAGCGCGGACGCACGCTAACGCCGAGATCCGCCGTCGCGCCTGCTTTaaa                                                               <  1:218468/140‑1 (MQ=255)                                                                 gCCCGTGGTGTTGCTTTAAGTGAGGACGGTGCCGGAAATTGCTCTCCGGCACCCAATAGTTTTACGCAGCCGGCAACTCCGCCAGCGGCCAGCGCGGACGCACGCTAACGCCGAGATCCGCCGTCGCGCCTGCTTTAAAc                                                              >  2:722592/1‑140 (MQ=255)                                                                  cccGTGGTGTTGCTTTAAGTGAGGACGGTGCCGGAAATTGCTCTCCGGCACCCAATAGTTTTACGCAGCCGGCAACTCCGCCAGCGGCCAGCGCGGACGCACGCTAACGCCGAGATCCGCCGTCGCGCCTGCTTTAAAcc                                                             >  1:1038251/1‑140 (MQ=255)                                                                   ccGTGGTGTTGCTTTAAGTGAGGACGGTGCCGGAAATTGCTCTCCGGCACCCAATAGTTTTACGCAGCCGGCAACTCCGCCAGCGGCCAGCGCGGACGCACGCTAACGCCGAGATCCGCCGTCGCGCCTGCTTTAAACCg                                                            >  1:1126988/1‑140 (MQ=255)                                                                    cGTGGTGTTGCTTTAAGTGAGGACGGTGCCGGAAATTGCTCTCCGGCACCCAATAGTTTTACGCAGCCGGCAACTCCGCCAGCGGCTAGCGCGGACGCACGCTAACGCCGAGATCCGCCGTTGCGCCTGCTTTAAACCGc                                                           >  1:157330/1‑140 (MQ=255)                                                                    cGTGGTGTTGCTTTAAGTGAGGACGGTGCCGGAAATTGCTCTCCGGCACCCAATAGTTTTACGCAGCCGGCAACTCCGCCAGCGGCCAGCGCGGACGCACGCTAACGCCGAGATCCGCCGTCgcgc                                                                         <  1:873182/126‑1 (MQ=255)                                                                    cGTGGTGTTGCTTTAAGTGAGGACGGTGCCGGAAATTGCTCTCCGGCACCCAATAGTTTTACGCAGCCGGCAACTCCGCCAGCGGCCAGCGCGGACGCACGCTAACGCCGAGATCCGCCGTCgcgc                                                                         >  2:873182/1‑126 (MQ=255)                                                                    cGTGGTGTTGCTTTAAGTGAGGACGGTGCCGGAAATTGCTCTCCGGCACCCAATAGTTTTACGCAGCCGGCAACTCCGCCAGCGGCCAGCGCGGACGCACGCTAACGCCGAGATCCGCCGTCGCGCCTg                                                                      <  1:1029228/129‑1 (MQ=255)                                                                    cGTGGTGTTGCTTTAAGTGAGGACGGTGCCGGAAATTGCTCTCCGGCACCCAATAGTTTTACGCAGCCGGCAACTCCGCCAGCGGCCAGCGCGGACGCACGCTAACGCCGAGATCCGCCGTCGCGCCTg                                                                      >  2:1029228/1‑129 (MQ=255)                                                                     gtggtgTTGCTTTAAGTGAGGACGGTGCCGGAAATTGCTCTCCGGCACCCAATAGTTTTACGCAGCCGGCAACTCCGCCAGCGGCCAGCGCGGACGCACGCTAACGCCGAGATCCGCCGTCGCGCCTGCTTTAAACCGCa                                                          <  1:371761/140‑1 (MQ=255)                                                                     gtggtgTTGCTTTAAGTGAGGACGGTGCCGGAAATTGCTCTCCGGCACCCAATAGTTTTACGCAGCCGGCAACTCCGCCAGCGGCCAGCGCGGACGCACGCTAACGCCGAGATCCGCCGTCGCGCCTGCTTTAAACCGCa                                                          <  2:282489/140‑1 (MQ=255)                                                                     gtggtgTTGCTTTAAGTGAGGACGGTGCCGGAAATTGCTCTCCGGCACCCAATAGTTTTACGCAGCCGGCAACTCCGCCAGCGGCCAGCGCGGACGCACGCTAACGCCGAGATCCGCCGTCGCGCCTGCTTTAAACCGCa                                                          <  1:957982/140‑1 (MQ=255)                                                                     gtggtgTTGCTTTAAGTGAGGACGGTGCCGGAAATTGCTCTCCGGCACCCAATAGTTTTACGCAGCCGGCAACTCCGCCAGCGGCCAGCGCGGACGCACGCTAACGCCGAGATCCGCCGTCGCGCCTGCTTTAAACCGCa                                                          <  1:757827/140‑1 (MQ=255)                                                                     gtggtgTTGCTTTAAGTGAGGACGGTGCCGGAAATTGCTCTCCGGCACCCAATAGTTTTACGCAGCCGGCAACTCCGCCAGCGGCCAGCGCGGACGCACGCTAACGCCGAGATCCGCCGTCGCGCCTGCTTTAAACCGCa                                                          <  2:1259511/140‑1 (MQ=255)                                                                      tggtgTTGCTTTAAGTGAGGACGGTGCCGGAAATTGCTCTCCGGCACCCAATAGTTTTACGCAGCCGGCAACTCCGCCAGCGGCCAGCGCGGACGCACGCTAACGCCGAGATCCGCCGTCGCGCCTGCTTTAAACCGCAc                                                         <  1:996691/140‑1 (MQ=255)                                                                      tggtgTTGCTTTAAGTGAGGACGGTGCCGGAAATTGCTCTCCGGCACCCAATAGTTTTACGCAGCCGGCAACTCCGCCAGCGGCCAGCGCGGACGCACGCTAACGCCGAGATCCGCCGTCGCGCCTGCTTTAAACCGCAc                                                         <  1:122087/140‑1 (MQ=255)                                                                             gCTTTAAGTGAGGACGGTGCCGGAAATTGCTCTCCGGCACCCAATAGTTTTACGCAGCCGGCAACTCCGCCAGCGGCCAGCGCGGACGCACGCTAACGCCGAGATCCGCCGTCGCGCCTGCTTTAAACCGCa                                                          >  1:807554/1‑132 (MQ=255)                                                                             gCTTTAAGTGAGGACGGTGCCGGAAATTGCTCTCCGGCACCCAATAGTTTTACGCAGCCGGCAACTCCGCCAGCGGCCAGCGCGGACGCACGCTAACGCCGAGATCCGCCGTCGCGCCTGCTTTAAACCGCa                                                          <  2:807554/132‑1 (MQ=255)                                                                             gCTTTAAGTGAGGACGGTGCCGGAAATTGCTCTCCGGCACCCAATAGTTTTACGCAGCCGGCAACTCCGCCAGCGGCCAGCGCGGACGCACGCTAACGCCGAGATCCGCCGTCGCGCCTGCTTTAAACCGCACCATTCCg                                                  >  1:338496/1‑140 (MQ=255)                                                                                ttAAGTGAGGACGGTGCCGGAAATTGCTCTCCGGCACCCAATAGTTTTACGCAGCCGGCAACTCCGCCAGCGGCCAGCGCGGACGCACGCTAACGCCGAGATCCGCCGTCGCGCCTGCTTTAAACCGCACCATTCCGGCa                                               <  2:301474/140‑1 (MQ=255)                                                                                   aGTGAGGACGGTGCCGGAAATTGCTCTCCGGCACCCAATAGTTTTACGCAGCCGGCAACTCCGCCAGCGGCCAGCGCGGACGCACGCTAACGCCGAGATCCGCCGTCGCGCCTGCTTTAAACCGCa                                                          <  2:411339/126‑1 (MQ=255)                                                                                   aGTGAGGACGGTGCCGGAAATTGCTCTCCGGCACCCAATAGTTTTACGCAGCCGGCAACTCCGCCAGCGGCCAGCGCGGACGCACGCTAACGCCGAGATCCGCCGTCGCGCCTGCTTTAAACCGCa                                                          >  1:411339/1‑126 (MQ=255)                                                                                   aGTGAGGACGGTGCCGGAAATTGCTCTCCGGCACCCAATAGTTTTACGCAGCCGGCAACTCCGCCAGCGGCCAGCGCGGACGCACGCTAACGCCGAGATCCGCCGTCGCGCCTGCTTTAAACCGCAc                                                         <  1:6909/127‑1 (MQ=255)                                                                                   aGTGAGGACGGTGCCGGAAATTGCTCTCCGGCACCCAATAGTTTTACGCAGCCGGCAACTCCGCCAGCGGCCAGCGCGGACGCACGCTAACGCCGAGATCCGCCGTCGCGCCTGCTTTAAACCGCAc                                                         >  2:6909/1‑127 (MQ=255)                                                                                   aGTGAGGACGGTGCCGGAAATTGCTCTCCGGCACCCAATAGTTTTACGCAGCCGGCAACTCCGCCAGCGGCCAGCGCGGACGCACGCTAACGCCGAGATCCGCCGTCGCGCCTGCTTTAAACCGCACCATTCCGGCATAg                                            >  1:761726/1‑140 (MQ=255)                                                                                   aGTGAGGACGGTGCCGGAAATTGCTCTCCGGCACCCAATAGTTTTACGCAGCCGGCAACTCCGCCAGCGGCCAGCGCGGACGCACGCTAACGCCGAGATCCGCCGTCGCGCCTGCTTTAAACCGCACCATTCCGGCATAg                                            >  1:1026055/1‑140 (MQ=255)                                                                                       aGGACGGTGCCGGAAATTGCTCTCCGGCACCCAATAGTTTTACGCAGCCGGCAACTCCGCCAGCGGCCAGCGCGGACGCACGCTAACGCCGAGATCCGCCGTCGCGCCTGCTTTAAACCGCACCATTCCGGCATAGGCGa                                        >  2:889422/1‑140 (MQ=255)                                                                                        ggACGGTGCCGGAAATTGCTCTCCGGCACCCAATAGTTTTACGCAGCCGGCAACTCCGCCAGCGGCCAGCGCGGACGCACGCTAACGCCGAGATCCGCCGTCGCGCCTGCTTTAAACCGCACCATTCCGGCATAGGCGat                                       >  2:731895/1‑140 (MQ=255)                                                                                        ggACGGTGCCGGAAATTGCTCTCCGGCACCCAATAGTTTTACGCAGCCGGCAACTCCGCCAGCGGCCAGCGCGGACGCACGCTAACGCCGAGATCCGCCGTCGCGCCTGCTTTAAACCGCACCATTCCGGCATAGGCGat                                       >  2:383671/1‑140 (MQ=255)                                                                                            ggTGCCGGAAATTGCTCTCCGGCACCCAATAGTTTTACGCAGCCGGCAACTCCGCCAGCGGCCAGCGCGGACGCACGCTAACGCCGAGATCCGCCGTCGCGCCTGCTTTAAACCGCACCATTCCGGCATAGGCGatcatc                                   <  2:113708/140‑1 (MQ=255)                                                                                            ggTGCCGGAAATTGCTCTCCGGCACCCAATAGTTTTACGCAGCCGGCAACTCCGCCAGCGGCCAGCGCGGACGCACGCTAACGCCGAGATCCGCCGTCGCGCCTGCTTTAAACCGCACCATTCCGGCATAGGCGatcatc                                   <  2:721531/140‑1 (MQ=255)                                                                                            ggTGCCGGAAATTGCTCTCCGGCACCCAATAGTTTTACGCAGCCGGCAACTCCGCCAGCGGCCAGCGCGGACGCACGCTAACGCCGAGATCCGCCGTCGCGCCTGCTTTAAACCGCACCATTCCGGCATAGGCGatcatc                                   <  2:848778/140‑1 (MQ=255)                                                                                            ggTGCCGGAAATTGCTCTCCGGCACCCAATAGTTTTACGCAGCCGGCAACTCCGCCAGCGGCCAGCGCGGACGCACGCTAACGCCGAGATCCGCCGTCGCGCCTGCTTTAAACCGCACCATTCCGGCATAGGCGatcatc                                   >  2:224422/1‑140 (MQ=255)                                                                                            ggTGCCGGAAATTGCTCTCCGGCACCCAATAGTTTTACGCAGCCGGCAACTCCGCCAGCGGCCAGCGCGGACGCACGCTAACGCCGAGATCCGCCGTCGCGCCTGCTTTAAACCGCACCATTCCGGCATAGGCGatcatc                                   <  1:1217571/140‑1 (MQ=255)                                                                                             gTGCCGGAAATTGCTCTCCGGCACCCAATAGTTTTACGCAGCCGGCAACTCCGCCAGCGGCCAGCGCGGACGCACGCTAACGCCGAGATCCGCCGTCGCGCCTGCTTTAAACCGCACCATTCCGGCATAGGCGATCATcg                                  <  2:898932/140‑1 (MQ=255)                                                                                             gTGCCGGAAATTGCTCTCCGGCACCCAATAGTTTTACGCAGCCGGCAACTCCGCCAGCGGCCAGCGCGGACGCACGCTAACGCCGAGATCCGCCGTCGCGCCTGCTTTAAACCGCACCATTCCGGCATAGGCGATCATcg                                  >  1:401429/1‑140 (MQ=255)                                                                                              tGCCGGAAATTGCTCTCCGGCACCCAATAGTTTTACGCAGCCGGCAACTCCGCCAGCGGCCAGCGCGGACGCACGCTAACGCCGAGATCCGCCGTCGCGCCTGCTTTAAACCGCACCATTCCGGCATAGGCGATCATcgc                                 >  1:1030421/1‑140 (MQ=255)                                                                                              tGCCGGAAATTGCTCTCCGGCA‑CCAATAGTTTTACGCAGCCGGCAACTCCGCCAGCGGCCAGCGCGGACGCACGCTAACGCCGAGATCCGCCGTCGCGCCTGCTTTAAACCGCACCATTCCGGCATAGGCGATCATcgcg                                <  1:1257155/140‑1 (MQ=255)                                                                                               gCCGGAAATTGCTCTCCGGCACCCAATAGTTTTACGCAGCCGGCAACTCCGCCAGCGGCCAGCGCGGACGCACGCTAACGCCGAGATCCGCCGTCGCGCCTGCTTTAAACCGCACCATTCCGGCATAGGCGATCATcgcg                                >  2:191403/1‑140 (MQ=255)                                                                                                   gAAATTGCTCTCCGGCACCCAATAGTTTTACGCAGCCGGCAACTCCGCCAGCGGCCAGCGCGGACGCACGCTAACGCCGAGATCCGCCGTCGCGCCTGCTTTAAACCGCACCATTCCGGCATAGGCGATCATCGCGCCGt                            <  2:1126988/140‑1 (MQ=255)                                                                                                      aTTGCTCTCCGGCACCCAATAGTTTTACGCAGCCGGCAACTCCGCCAGCGGCCAGCGCGGACGCACGCTAACGCCGAGATCCGCCGTCGCGCCTGCTTTAAACCGCACCATTCCGGCATAGGCGATCATCGCGCCGTTAt                         <  1:889422/140‑1 (MQ=255)                                                                                                      aTTGCTCTCCGGCACCCAATAGTTTTACGCAGCCGGCAACTCCGCCAGCGGCCAGCGCGGACGCACGCTAACGCCGAGATCCGCCGTCGCGCCTGCTTTAAACCGCACCATTCCGGCATAGGCGATCATCGCGCCGTTAt                         <  1:95162/140‑1 (MQ=255)                                                                                                          ctctCCGGCACCCAATAGTTTTACGCAGCCGGCAACTCCGCCAGCGGCCAGCGCGGACGCACGCTAACGCCGAGATCCGCCGTCGCGCCTGCTTTAAACCGCACCATTCCGGCATAGGCGATCATCGCGCCGTTATCAGt                     >  2:399478/1‑140 (MQ=255)                                                                                                          ctctCCGGCACCCAATAGTTTTACGCAGCCGGCAACTCCGCCAGCGGCCAGCGCGGACGCACGCTAACGCCGAGATCCGCCGTCGCGCCTGCTTTAAACCGCACCATTCCGGCATAGGCGATCATCGCGCCGTTATCAGt                     >  2:277027/1‑140 (MQ=255)                                                                                                                  cACCCAATAGTTTTACGCAGCCGGCAACTCCGCCAGCGGCCAGCGCGGACGCACGCTAACGCCGAGATCCGCCGTCGCGCCTGCTTTAAACCGCACCATTCCGGCATAGGCGATCATCGCGCCGTTATCAGTACAAAAtt             >  2:752440/1‑140 (MQ=255)                                                                                                                  cACCCAATAGTTTTACGCAGCCGGCAACTCCGCCAGCGGCCAGCGCGGACGCACGCTAACGCCGAGATCCGCCGTCGCGCCTGCTTTAAACCGCACCATTCCGGCATAGGCGATCATCGCGCCGTTATCAGTACAAAAtt             >  2:1078035/1‑140 (MQ=255)                                                                                                                     ccAATAGTTTTACGCAGCCGGCAACTCCGCCAGCGGCCAGCGCGGACGCACGCTAACGCCGAGATCCGCCGTCGCGCCTGCTTTAAACCGCACCATTCCGGCATAGGCGATCATCGCGCCGTTATCAGTACAAAATTCCg          <  1:191403/140‑1 (MQ=255)                                                                                                                     ccAATAGTTTTACGCAGCCGGCAACTCCGCCAGCGGCCAGCGCGGACGCACGCTAACGCCGAGATCCGCCGTCGCGCCTGCTTTAAACCGCACCATTCCGGCATAGGCGATCATCGCGCCGTTATCAGTACAAAATTCCg          <  1:712873/140‑1 (MQ=255)                                                                                                                       aaTAGTTTTACGCAGCCGGCAACTCCGCCAGCGGCCAGCGCGGACGCACGCTAACGCCGAGATCCGCCGTCGCGCCTGCTTTAAACCGCACCATTCCGGCATAGGCGATCATCGCGCCGTTATCAGTACAAAATTCCGGa        <  1:32484/140‑1 (MQ=255)                                                                                                                       aaTAGTTTTACGCAGCCGGCAACTCCGCCAGCGGCCAGCGCGGACGCACGCTAACGCCGAGATCCGCCGTCGCGCCTGCTTTAAACCGCACCATTCCGGCATAGGCGATCATCGCGCCGTTATCAGTACAAAATTCCGGa        <  1:926636/140‑1 (MQ=255)                                                                                                                        aTAGTTTTACGCAGCCGGCAACTCCGCCAGCGGCCAGCGCGGACGCACGCTAACGCCGAGATCCGCCGTCGCGCCTGCTTTAAACCGCACCATTCCGGCATAGGCGATCATCGCGCCGTTATCAGTACAAAATTCCGGAc       <  1:277027/140‑1 (MQ=255)                                                                                                                         tAGTTTTACGCAGCCGGCAACTCCGCCAGCGGCCAGCGCGGACGCACGCTAACGCCGAGATCCGCCGTCGCGCCTGCTTTAAACCGCACCATTCCGGCATAGGCGATCATCGCGCCGTTATCAGTACAAAATTCCGGAcg      >  1:1024344/1‑140 (MQ=255)                                                                                                                          aGTTTTACGCAGCCGGCAACTCCGCCAGCGGCCAGCGCGGACGCACGCTAACGCCGAGATCCGCCGTCGCGCCTGCTTTAAACCGCACCATTCCGGCATAGGCGATCATCGCGCCGTTATCAGTACAAAATTCCGGAcgc     <  2:150958/140‑1 (MQ=255)                                                                                                                             tttACGCAGCCGGCAACTCCGCCAGCGGCCAGCGCGGACGCACGCTAACGCCGAGATCCGCCGTCGCGCCTGCTTTAAACCGCACCATTCCGGCATAGGCGATCATCGCGCCGTTATCAGTACAAAATTCCGGAcgcgcg  <  2:401429/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                               GACTGGGGGCGATTTTTGGGCTGATATTCCTCGTATTGCTGGTGATTACCGGCTTACTGTGGATGCCCGTGGTGTTGCTTTAAGTGAGGACGGTGCCGGAAATTGCTCTCCGGCACCCAATAGTTTTACGCAGCCGGTAACTCCGCCAGCGGCCAGCGCGGACGCACGCTAACGCCGAGATCCGCCGTCGCGCCTGCTTTAAACCGCACCATTCCGGCATAGGCGATCATCGCGCCGTTATCAGTACAAAATTCCGGACGCGCG  >  NC_000913/3209405‑3209668

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑