Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,254,028	A→G	F63L (TTC→CTC)	yhaJ ←	DNA‑binding transcriptional activator YhaJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,254,028	0	A	G	100.0%	250.7 / NA	69	F63L (TTC→CTC)	yhaJ	DNA‑binding transcriptional activator YhaJ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (37/32);  total (37/32)

GTACCAGCGCTTCGGTCACAATGGTGAGATGCGTTTCCCAACCGCGCGCGAGAGCTTCCGCATCAGTAGTCAGTTTATCTGCGGCTTCCAGCAAAACGCGCCCTCGCTCCAACAACATCCTCCCGACATTGGTGAATTTGGTACGATGGCCCGAGCGGTCAAACAGCACCACATCCAGCTCTTCTTCCAGTTTTTGCATGGTGTAGCTAAGTGCGGAAGGCACGCGTCCCAGCTCATCCGCCGCCGCCGCAAAACTGCCCCGGCGATCGAT  >  NC_000913/3253893‑3254163                                                                                                                                        |                                                                                                                                        gTACCAGCGCTTCGGTCACAATGGTGAGATGCGTTTCCCAACCGCGCGCGAGAGCTTCCGCATCAGTAGTCAGTTTATCTGCGGCTTCCAGCAAAACGCGCCCTCGCTCCAACAACATCCTCCCGACATTGGTGAGTTTg                                                                                                                                     <  1:14202/140‑1 (MQ=255)      aGCGCTTCGGTCACAATGGTGAGATGCGTTTCCCAACCGCGCGCGAGAGCTTCCGCATCAGTAGTCAGTTTATCTGCGGCTTCCAGCAAAACGCGCCCTCGCTCCAACAACATCCTCCCGACATTGGTGAGTTTGGTACg                                                                                                                                >  2:819097/1‑140 (MQ=255)       gcgcTTCGGTCACAATGGTGAGATGCGTTTCCCAACCGCGCGCGAGAGCTTCCGCATCAGTAGTCAGTTTATCTGCGGCTTCCAGCAAAACGCGCCCTCGCTCCAACAACATCCTCCCGACATTGGTGAGTTTGGTACGa                                                                                                                               >  1:700536/1‑140 (MQ=255)          cTTCGGTCACAATGGTGAGATGCGTTTCCCAACCGCGCGCGAGAGCTTCCGCATCAGTAGTCAGTTTATCTGCGGCTTCCAGCAAAACGCGCCCTCGCTCCAACAACATCCTCCCGACATTGGTGAGTTTGGTACGATgg                                                                                                                            >  2:893330/1‑140 (MQ=255)            tCGGTCACAATGGTGAGATGCGTTTCCCAACCGCGCGCGAGAGCTTCCGCATCAGTAGTCAGTTTATCTGCGGCTTCCAGCAAAACGCGCCCTCGCTCCAACAACATCCTCCCGACATTGGTGAGTTTGGTACGATGGcc                                                                                                                          >  1:1357463/1‑140 (MQ=255)              ggTCACAATGGTGAGATGCGTTTCCCAACCGCGCGCGAGAGCTTCCGCATCAGTAGTCAGTTTATCTGCGGCTTCCAGCAAAACGCGCCCTCGCTCCAACAACATCCTCCCGACATTGGTGAGTTTGGTACGATGGCCCg                                                                                                                        <  2:517873/140‑1 (MQ=255)                  acaATGGTGAGATGCGTTTCCCAACCGCGCGCGAGAGCTTCCGCATCAGTAGTCAGTTTATCTGCGGCTTCCAGCAAAACGCGCCCTCGCTCCAACAACATCCTCCCGACATTGGTGAGTTTGGTACGATGGCCCGAGCg                                                                                                                    >  1:776771/1‑140 (MQ=255)                   caATGGTGAGATGCGTTTCCCAACCGCGCGCGAGAGCTTCCGCATCAGTAGTCAGTTTATCTGCGGCTTCCAGCAAAACGCGCCCTCGCTCCAACAACATCCTCCCGACATTGGTGAGTTTGGTACGATGGCCCGAGCgg                                                                                                                   <  1:36813/140‑1 (MQ=255)                         tGAGATGCGTTTCCCAACCGCGCGCGAGAGCTTCCGCATCAGTAGTCAGTTTATCTGCGGCTTCCAGCAAAACGCGCCCTCGCTCCAACAACATCCTCCCGACATTGGTGAGTTTGGTACGATGGCCCg                                                                                                                        >  1:802878/1‑129 (MQ=255)                         tGAGATGCGTTTCCCAACCGCGCGCGAGAGCTTCCGCATCAGTAGTCAGTTTATCTGCGGCTTCCAGCAAAACGCGCCCTCGCTCCAACAACATCCTCCCGACATTGGTGAGTTTGGTACGATGGCCCg                                                                                                                        <  2:802878/129‑1 (MQ=255)                          gagaTGCGTTTCCCAACCGCGCGCGAGAGCTTCCGCATCAGTAGTCAGTTTATCTGCGGCTTCCAGCAAAACGCGCCCTCGCTCCAACAACATCCTCCCGACATTGGTGAGTTTGGTACGATGGCCCGAGCGGTCAAACa                                                                                                            <  1:1230490/140‑1 (MQ=255)                                cGTTTCCCAACCGCGCGCGAGAGCTTCCGCATCAGTAGTCAGTTTATCTGCGGCTTCCAGCAAAACGCGCCCTCGCTCCAACAACATCCTCCCGACATTGGTGAGTTTGGTACGATGGCCCGAGCGGTCAAACAGcac                                                                                                        >  2:246479/1‑138 (MQ=255)                                cGTTTCCCAACCGCGCGCGAGAGCTTCCGCATCAGTAGTCAGTTTATCTGCGGCTTCCAGCAAAACGCGCCCTCGCTCCAACAACATCCTCCCGACATTGGTGAGTTTGGTACGATGGCCCGAGCGGTCAAACAGcac                                                                                                        <  1:246479/138‑1 (MQ=255)                                cGTTTCCCAACCGCGCGCGAGAGCTTCCGCATCAGTAGTCAGTTTATCTGCGGCTTCCAGCAAAACGCGCCCTCGCTCCAACAACATCCTCCCGACATTGGTGAGTTTGGTACGATGGCCCGAGCGGTCAAACAGca                                                                                                         >  2:968847/1‑137 (MQ=255)                                cGTTTCCCAACCGCGCGCGAGAGCTTCCGCATCAGTAGTCAGTTTATCTGCGGCTTCCAGCAAAACGCGCCCTCGCTCCAACAACATCCTCCCGACATTGGTGAGTTTGGTACGATGGCCCGAGCGGTCAAACAGca                                                                                                         <  1:968847/137‑1 (MQ=255)                                         aCCGCGCGCGAGAGCTTCCGCATCAGTAGTCAGTTTATCTGCGGCTTCCAGCAAAACGCGCCCTCGCTCCAACAACATCCTCCCGACATTGGTGAGTTTGGTACGATGGCCCGAGCGGTCAAACAGCACCACATCCAGct                                                                                             <  2:707211/140‑1 (MQ=255)                                          ccGCGCGCGAGAGCTTCCGCATCAGTAGTCAGTTTATCTGCGGCTTCCAGCAAAACGCGCCCTCGCTCCAACAACATCCTCCCGACATTGGTGAGTTTGGTACGATGGCCCGAGCGGTCAAACAGCACCACATCCAGctc                                                                                            >  2:678180/1‑140 (MQ=255)                                             cgcgcgAGAGCTTCCGCATCAGTAGTCAGTTTATCTGCGGCTTCCAGCAAAACGCGCCCTCGCTCCAACAACATCCTCCCGACATTGGTGAGTTTGGTACGATGGCCCGAGCGGTCAAACAGCACCACATCCAGCtcttc                                                                                         >  1:1388600/1‑140 (MQ=255)                                             cgcgcgAGAGCTTCCGCATCAGTAGTCAGTTTATCTGCGGCTTCCAGCAAAACGCGCCCTCGCTCCAACAACATCCTCCCGACATTGGTGAGTTTGGTACGATGGCCCGAGCGGTCAAACAGCACCACATCCAGCtcttc                                                                                         >  2:355657/1‑140 (MQ=255)                                             cgcgcgAGAGCTTCCGCATCAGTAGTCAGTTTATCTGCGGCTTCCAGCAAAACGCGCCCTCGCTCCAACAACATCCTCCCGACATTGGTGAGTTTGGTACGATGGCCCGAGCGGTCAAACAGCACCACATCCAGCtcttc                                                                                         >  1:1301306/1‑140 (MQ=255)                                             cgcgcgAGAGCTTCCGCATCAGTAGTCAGTTTATCTGCGGCTTCCAGCAAAACGCGCCCTCGCTCCAACAACATCCTCCCGACATTGGTGAGTTTGGTACGATGGCCCGAGCGGTCAAACAGCACCACATCCAGCtcttc                                                                                         >  2:866794/1‑140 (MQ=255)                                             cgcgcgAGAGCTTCCGCATCAGTAGTCAGTTTATCTGCGGCTTCCAGCAAAACGCGCCCTCGCTCCAACAACATCCTCCCGACATTGGTGAGTTTGGTACGATGGCCCGAGCGGTCAAACAGCACCACATCCAGCtcttc                                                                                         >  1:1270810/1‑140 (MQ=255)                                             cgcgcgAGAGCTTCCGCATCAGTAGTCAGTTTATCTGCGGCTTCCAGCAAAACGCGCCCTCGCTCCAACAACATCCTCCCGACATTGGTGAGTTTGGTACGATGGCCCGAGCGGTCAAACAGCACCACATCCAGCtcttc                                                                                         >  1:1160061/1‑140 (MQ=255)                                               cgcgAGAGCTTCCGCATCAGTAGTCAGTTTATCTGCGGCTTCCAGCAAAACGCGCCCTCGCTCCAGCAACATCCTCCCGACATTGGTGAGTTTGGTACGATGGCCCGAGCGGTCAAACAGCACCACATCCAGCTcttctt                                                                                       >  1:1636/1‑140 (MQ=255)                                                          ccGCATCAGTAGTCAGTTTATCTGCGGCTTCCAGCAAAACGCGCCCTCGCTCCAACAACATCCTCCCGACATTGGTGAGTTTGGTACGATGGCCCGAGCGGTCAAACAGCACCACATCCAGCTCTTCTTCCAGTTTTTGc                                                                            <  2:753201/140‑1 (MQ=255)                                                           cGCATCAGTAGTCAGTTTATCTGCGGCTTCCAGCAAAACGCGCCCTCGCTCCAACAACATCCTCCCGACATTGGTGAGTTTGGTACGATGGCCCGAGCGGTCAAACAGCACCACATCCAGCTCTTCTTCCAGTTTTTGCa                                                                           <  1:515886/140‑1 (MQ=255)                                                           cGCATCAGTAGTCAGTTTATCTGCGGCTTCCAGCAAAACGCGCCCTCGCTCCAACAACATCCTCCCGACATTGGTGAGTTTGGTACGATGGCCCGAGCGGTCAAACAGCACCACATCCAGCTCTTCTTCCAGTTTTTGCa                                                                           <  2:1270810/140‑1 (MQ=255)                                                           cGCATCAGTAGTCAGTTTATCTGCGGCTTCCAGCAAAACGCGCCCTCGCTCCAACAACATCCTCCCGACATTGGTGAGTTTGGTACGATGGCCCGAGCGGTCAAACAGCACCACATCCAGCTCTTCTTCCAGTTTTTGCa                                                                           <  2:52694/140‑1 (MQ=255)                                                           cGCATCAGTAGTCAGTTTATCTGCGGCTTCCAGCAAAACGCGCCCTCGCTCCAACAACATCCTCCCGACATTGGTGAGTTTGGTACGATGGCCCGAGCGGTCAAACAGCACCACATCCAGCTCTTCTTCCAGTTTTTGCa                                                                           <  2:1124427/140‑1 (MQ=255)                                                           cGCATCAGTAGTCAGTTTATCTGCGGCTTCCAGCAAAACGCGCCCTCGCTCCAACAACATCCTCCCGACATTGGTGAGTTTGGTACGATGGCCCGAGCGGTCAAACAGCACCACATCCAGCTCTTCTTCCAGTTTTTGCa                                                                           <  2:1357463/140‑1 (MQ=255)                                                           cGCATCAGTAGTCAGTTTATCTGCGGCTTCCAGCAAAACGCGCCCTCGCTCCAACAACATCCTCCCGACATTGGTGAGTTTGGTACGATGGCCCGAGCGGTCAAACAGCACCACATCCAGCTCTTCTTCCAGTTTTTGCa                                                                           <  2:951427/140‑1 (MQ=255)                                                           cGCATCAGTAGTCAGTTTATCTGCGGCTTCCAGCAAAACGCGCCCTCGCTCCAACAACATCCTCCCGACATTGGTGAGTTTGGTACGATGGCCCGAGCGGTCAAACAGCACCACATCCAGCTCTTCTTCCAGTTTTTGCa                                                                           <  1:454941/140‑1 (MQ=255)                                                           cGCATCAGTAGTCAGTTTATCTGCGGCTTCCAGCAAAACGCGCCCTCGCTCCAACAACATCCTCCCGACATTGGTGAGTTTGGTACGATGGCCCGAGCGGTCAAACAGCACCACATCCAGCTCTTCTTCCAGTTTTTGCa                                                                           <  2:1388600/140‑1 (MQ=255)                                                           cGCATCAGTAGTCAGTTTATCTGCGGCTTCCAGCAAAACGCGCCCTCGCTCCAACAACATCCTCCCGACATTGGTGAGTTTGGTACGATGGCCCGAGCGGTCAAACAGCACCACATCCAGCTCTTCTTCCAGTTTTTGCa                                                                           <  1:166402/140‑1 (MQ=255)                                                           cGCATCAGTAGTCAGTTTATCTGCGGCTTCCAGCAAAACGCGCCCTCGCTCCAACAACATCCTCCCGACATTGGTGAGTTTGGTACGATGGCCCGAGCGGTCAAACAGCACCACATCCAGCTCTTCTTCCAGTTTTTGCa                                                                           <  2:306687/140‑1 (MQ=255)                                                           cGCATCAGTAGTCAGTTTATCTGCGGCTTCCAGCAAAACGCGCCCTCGCTCCAACAACATCCTCCCGACATTGATGAGTTTGGTACGATGGCCCGAGCGGTCAAACAGCCCCACATCCAGCTCTTCTTCCAGTTTTTGCa                                                                           <  2:248890/140‑1 (MQ=255)                                                                        aGTTTATCTGCGGCTTCCAGCAAAACGCGCCCTCGCTCCAACAACATCCTCCCGACATTGGTGAGTTTGGTACGATGGCCCGAGCGGTCAAACAGCACCACATCCAGCTCTTCTTCCAGTTTTTGCATGGTGTAGCTAAg                                                              >  1:741077/1‑140 (MQ=255)                                                                          tttATCTGCGGCTTCCAGCAAAACGCGCCCTCGCTCCAACAACATCCTCCCGACGTTGGTGAGTTTGGTACGATGGCCCGAGCGGTCAAACAGCACCACATCCAGCTCTTCTTCCAGTTTTTGCATGGTGTAGCTAAGTg                                                            >  2:316391/1‑140 (MQ=255)                                                                          tttATCTGCGGCTTCCAGCAAAACGCGCCCTCGCTCCAACAACATCCTCCCGACATTGGTGAGTTTGGTACGATGGCCCGAGCGGTCAAACAGCACCACATCCAGCTCTTCTTCCAGTTTTTGCa                                                                           <  2:1018918/125‑1 (MQ=255)                                                                          tttATCTGCGGCTTCCAGCAAAACGCGCCCTCGCTCCAACAACATCCTCCCGACATTGGTGAGTTTGGTACGATGGCCCGAGCGGTCAAACAGCACCACATCCAGCTCTTCTTCCAGTTTTTGCa                                                                           >  1:1018918/1‑125 (MQ=255)                                                                          tttATCTGCGGCTTCCAGCAAAACGCGCCCTCGCTCCAACAACATCCTCCCGACATTGGTGAGTTTGGTACGATGGCCCGAGCGGTCAAACAGCACCACATCCAGCTCTTCTTCCAGTTTTTGCATg                                                                         <  2:1358607/127‑1 (MQ=255)                                                                          tttATCTGCGGCTTCCAGCAAAACGCGCCCTCGCTCCAACAACATCCTCCCGACATTGGTGAGTTTGGTACGATGGCCCGAGCGGTCAAACAGCACCACATCCAGCTCTTCTTCCAGTTTTTGCATg                                                                         >  1:1358607/1‑127 (MQ=255)                                                                          tttATCTGCGGCTTCCAGCAAAACGCGCCCTCGCTCCAACAACATCCTCCCGACATTGGTGAGTTTGGTACGATGGCCCGAGCGGTCAAACAGCACCACATCCAGCTCTTCTTCCAGTTTTTGCATGGTGTAGCTAAGTg                                                            <  1:352515/140‑1 (MQ=255)                                                                            tATCTGCGGCTTCCAGCAAAACGCGCCCTCGCTCCAGCAACATCCTCCCGACATTGGTGAGTTTGGTACGATGGCCCGAGCGGTCAAACAGCACCACATCCAGCTCTTCTTCCAGTTTTTGCATGGTGTAGCTAAGTGCg                                                          <  2:1636/140‑1 (MQ=255)                                                                             aTCTGCGGCTTCCAGCAAAACGCGCCCTCGCTCCAACAACATCCTCCCGACATTGGTGAGTTTGGTACGATGGCCCGAGCGGTCAAACAGCACCACATCCAGCTCTTCTTCCAGTTTTTGCATGGTGTAGCTAAGTGCgg                                                         <  2:741077/140‑1 (MQ=255)                                                                             aTCTGCGGCTTCCAGCAAAACGCGCCCTCGCTCCAACAACATCCTCCCGACATTGGTGAGTTTGGTACGATGGCCCGAGCGGTCAAACAGCACCACATCCAGCTCTTCTTCCAGTTTTTGCATGGTGTAGCTAAGTGCgg                                                         >  1:638061/1‑140 (MQ=255)                                                                             aTCTGCGGCTTCCAGCAAAACGCGCCCTCGCTCCAACAACATCCTCCCGACATTGGTGAGTTTGGTACGATGGCCCGAGCGGTCAAACAGCACCACATCCAGCTCTTCTTCCAGTTTTTGCATGGTGTAGCTAAGTGCgg                                                         >  1:121990/1‑140 (MQ=255)                                                                                    gCTTCCAGCAAAACGCGCCCTCGCTCCAACAACATCCTCCCGACATTGGTGAGTTTGGTACGATGGCCCGAGCGGTCAAACAGCACCACATCCAGCTCTTCTTCCAGTTTTTGCATGGTGTAGCTAAGTGCgg                                                         <  2:1381763/133‑1 (MQ=255)                                                                                    gCTTCCAGCAAAACGCGCCCTCGCTCCAACAACATCCTCCCGACATTGGTGAGTTTGGTACGATGGCCCGAGCGGTCAAACAGCACCACATCCAGCTCTTCTTCCAGTTTTTGCATGGTGTAGCTAAGTGCgg                                                         >  1:1381763/1‑133 (MQ=255)                                                                                    gCTTCCAGCAAAACGCGCCCTCGCTCCAACAACATCCTCCCGACATTGGTGAGTTTGGTACGATGGCCCGAGCGGTCAAACAGCACCACATCCAGCTCTTCTTCCAGTTTTTGCATGGTGTAGCTAAGTGCGGAAGGCAc                                                  >  1:1089094/1‑140 (MQ=255)                                                                                    gCTTCCAGCAAAACGCGCCCTCGCTCCAACAACATCCTCCCGACATTGGTGAGTTTGGTACGATGGCCCGAGCGGTCAAACAGCACCACATCCAGCTCTTCTTCCAGTTTTTGCATGGTGTAGCTAAGTGCGGAAGGCAc                                                  >  2:139450/1‑140 (MQ=255)                                                                                     cTTCCAGCAAAACGCGCCCTCGCTCCAACAACATCCTCCCGACATTGGTGAGTTTGGTACGATGGCCCGAGCGGTCAAACAGCACCACATCCAGCTCTTCTTCCAGTTTTTGCATGGTGTAGCTAAGTGCGGAAGGCAcg                                                 <  2:121990/140‑1 (MQ=255)                                                                                     cTTCCAGCAAAACGCGCCCTCGCTCCAACAACATCCTCCCGACATTGGTGAGTTTGGTACGATGGCCCGAGCGGTCAAACAGCACCACATCCAGCTCTTCTTCCAGTTTTTGCATGGTGTAGCTAAGTGCGGAAGGCAcg                                                 <  2:700536/140‑1 (MQ=255)                                                                                        ccAGCAAAACGCGCCCTCGCTCCAACAACATCCTCCCGACATTGGTGAGTTTGGTACGATGGCCCGAGCGGTCAAACAGCACCACATCCAGCTCTTCTTCCAGTTTTTGCATGGTGTAGCTAAGTGCGGAAGGCACGCGt                                              >  1:897450/1‑140 (MQ=255)                                                                                               aaCGCGCCCTCGCTCCAACAACATCCTCCCGACATTGGTGAGTTTGGTACGATGGCCCGAGCGGTCAAACAGCACCACATCCAGCTCTTCTTCCAGTTTTTGCATGGTGTAGCTAAGTGCGGAAGGCAcg                                                 <  1:926112/130‑1 (MQ=255)                                                                                               aaCGCGCCCTCGCTCCAACAACAGCCTCCCGACATTGGTGAGTTTGGTACGATGGCCCGAGCGGTCAAACAGCACCACATCCAGCTCTTCTTCCAGTTTTTGCATGGTGTAGCTAAGTGCGGAAGGCAcg                                                 >  2:926112/1‑130 (MQ=255)                                                                                                aCGCGCCCTCGCTCCAACAACATCCTCCCGACATTGGTGAGTTTGGTACGATGGCCCGAGCGGTCAAACAGCACCACATCCAGCTCTTCTTCCAGTTTTTGCATGGTGTAGCTAAGTGCGGAAGGCACGCGTCCCAGCTc                                      >  1:619245/1‑140 (MQ=255)                                                                                                      ccTCGCTCCAACAACATCCTCCCGACATTGGTGAGTTTGGTACGATGGCCCGAGCGGTCAAACAGCACCACATCCAGCTCTTCTTCCAGTTTTTGCATGGTGTAGCTAAGTGCGGAAGGCACGCGTCCCAGCTCATccgc                                >  1:723680/1‑140 (MQ=255)                                                                                                         cGCTCCAACAACATCCTCCCGACATTGGTGAGTTTGGTACGATGGCCCGAGCGGTCAAACAGCACCACATCCAGCTCTTCTTCCAGTTTTTGCATGGTGTAGCTAAGTGCGGAAGGCACGCGTCCCAGCTCATccgccgc                             <  1:866794/140‑1 (MQ=255)                                                                                                                      tcctccCGACATTGGTGAGTTTGGTACGATGGCCCGAGCGGTCAAACAGCACCACATCCAGTTCTTCTTCCAGTTTTTGCATGGTGTAGCTAAGTGCGGAAGGCACGCGTCCCAGCTCATCCGCCGCCGCCGCAAAACTg                >  2:1063144/1‑140 (MQ=255)                                                                                                                        ctccCGACATTGGTGAGTTTGGTACGATGGCCCGAGCGGTCAAACAGCACCACATCCAGCTCTTCTTCCAGTTTTTGCATGGTGTAGCTAAGTGCGGAAGGCACGCGTCCCAGCTCATCCGCCGCCGCCGCAAAACTGcc              >  2:1045304/1‑140 (MQ=255)                                                                                                                         tccCGACATTGGTGAGTTTGGTACGATGGCCCGAGCGGTCAAACAGCACCACATCCAGCTCTTCTTCCAGTTTTTGCATGGTGTAGCTAAGTGCGGAAGGCACGCGTCCCAGCTCATCCGCCGCCGCCGCAAAACTGccc             >  2:837162/1‑140 (MQ=255)                                                                                                                          cccGACATTGGTGAGTTTGGTACGATGGCCCGAGCGGTCAAACAGCACCACATCCAGCTCTTCTTCCAGTTTTTGCATGGTGTAGCTAAGTGCGGAAGGCACGCGTCCCAGCTCATCCGCCGCCGCCGCAAAACTGcccc            >  1:243291/1‑140 (MQ=255)                                                                                                                          cccGACATTGGTGAGTTTGGTACGATGGCCCGAGCGGTCAAACAGCACCACATCCAGCTCTTCTTCCAGTTTTTGCATGGTGTAGCTAAGTGCGGAAGGCACGCGTCCCAGCTCATCCGCCGCCGCCGCAAAACTGcccc            >  1:591597/1‑140 (MQ=255)                                                                                                                           ccGACATTGGTGAGTTTGGTACGATGGCCCGAGCGGTCAAACAGCACCACATCCAGCTCTTCTTCCAGTTTTTGCATGGTGTAGCTAAGTGCGGAAGGCACGCGTCCCAGCTCATCCGCCGCCGCCGCAAAACTGCCCCg           >  2:399952/1‑140 (MQ=255)                                                                                                                              aCATTGGTGAGTTTGGTACGATGGCCCGAGCGGTCAAACAGCACCACATCCAGCTCTTCTTCCAGTTTTTGCATGGTGTAGCTAAGTGCGGAAGGCACGCGTCCCAGCTCATCCGCCGCCGCCGCAAAACTGCCCCGGcg        >  2:386711/1‑140 (MQ=255)                                                                                                                                aTTGGTGAGTTTGGTACGATGGCCCGAGCGGTCAAACAGCACCACATCCAGCTCTTCTTCCAGTTTTTGCATGGTGTAGCTAAGTGCGGAAGGCACGCGTCCCAGCTCATCCGCCGCCGCCGCAAAACTGCCCCGGcgat      <  1:837162/140‑1 (MQ=255)                                                                                                                                aTTGGTGAGTTTGGTACGATGGCCCGAGCGGTCAAACAGCACCACATCCAGCTCTTCTTCCAGTTTTTGCATGGTGTAGCTAAGTGCGGAAGGCACGCGTCCCAGCTCATCCGCCGCCGCCGCAAAACTGCCCCGGcgat      <  2:619245/140‑1 (MQ=255)                                                                                                                                    gTGAGTTTGGTACGATGGCCCGAGCGGTCAAACAGCACCACATCCAGCTCTTCTTCCAGTTTTTGCATGGTGTAGCTAAGTGCGGAAGGCACGCGTCCCAGCTCATCCGCCGCCGCCGCAAAACTGCCCCGGcgatcgat  >  2:1099401/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                        GTACCAGCGCTTCGGTCACAATGGTGAGATGCGTTTCCCAACCGCGCGCGAGAGCTTCCGCATCAGTAGTCAGTTTATCTGCGGCTTCCAGCAAAACGCGCCCTCGCTCCAACAACATCCTCCCGACATTGGTGAATTTGGTACGATGGCCCGAGCGGTCAAACAGCACCACATCCAGCTCTTCTTCCAGTTTTTGCATGGTGTAGCTAAGTGCGGAAGGCACGCGTCCCAGCTCATCCGCCGCCGCCGCAAAACTGCCCCGGCGATCGAT  >  NC_000913/3253893‑3254163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑