Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,278,978	T→C	V22A (GTT→GCT)	kbaZ →	tagatose‑1,6‑bisphosphate aldolase 1 subunit KbaZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,278,978	0	T	C	100.0%	324.9 / NA	88	V22A (GTT→GCT)	kbaZ	tagatose‑1,6‑bisphosphate aldolase 1 subunit KbaZ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (44/44);  total (44/44)

ACCATGACGCAGTATCAACTGAAACAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTATGCCGTTTGTTCCGCACATCCGCTGGTGCTGGAAGCTGCAATCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATT  >  NC_000913/3278845‑3279112                                                                                                                                      |                                                                                                                                       aCCATGACGCAGTATCAACTGAAACAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTATGCCGCTTGTTc                                                                                                                                  <  1:1021306/140‑1 (MQ=255) aCCATGACGCAGTATCAACTGAAACAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTATGCCGCTTGTTc                                                                                                                                  >  1:625972/1‑140 (MQ=255)     tGACGCAGTATCAACTGAAACAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTATGCCGCTTGt                                                                                                                                    <  2:22058/134‑1 (MQ=255)     tGACGCAGTATCAACTGAAACAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTATGCCGCTTGt                                                                                                                                    >  1:22058/1‑134 (MQ=255)     tGACGCAGTATCAACTGAAACAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTATGCCGCTTGTTCCGca                                                                                                                              >  2:994885/1‑140 (MQ=255)           aGTATCAACTGAAACAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTATGCCGCTTGTTCCGCACATCCg                                                                                                                        >  1:1085573/1‑140 (MQ=255)           aGTATCAACTGAAACAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTATGCCGCTTGTTCCGCACATCCg                                                                                                                        >  1:454086/1‑140 (MQ=255)            gTATCAACTGAAACAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTATGCCGCTTGTTCCGCACATgcgc                                                                                                                       <  2:477497/140‑1 (MQ=255)            gTATCAACTGAAACAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTATGCCGCTTGTTCCGCACATCCGc                                                                                                                       <  2:363158/140‑1 (MQ=255)             tATCAACTGAAACAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTATGCCGCTTGTTCCGCACATCCg                                                                                                                        <  2:290302/138‑1 (MQ=255)             tATCAACTGAAACAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTATGCCGCTTGTTCCGCACATCCg                                                                                                                        >  1:290302/1‑138 (MQ=255)             tATCAACTGAAACAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTATGCCGCTTGTTCCGCACATCCGc                                                                                                                       <  1:1165250/139‑1 (MQ=255)             tATCAACTGAAACAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTATGCCGCTTGTTCCGCACATCCGc                                                                                                                       >  2:1165250/1‑139 (MQ=255)               tCAACTGAAACAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTATGCCGCTTGTTCCGCACATCCGCTgg                                                                                                                    >  2:1058696/1‑140 (MQ=255)                       aaCAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTATGCCGCTTGTTCCGCACATCCGCTGGTg                                                                                                                  <  1:250329/134‑1 (MQ=255)                       aaCAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTATGCCGCTTGTTCCGCACATCCGCTGGTg                                                                                                                  >  2:250329/1‑134 (MQ=255)                       aaCAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTATGCCGCTTGTTCCGCACATCCGCTGGTGCTGGaa                                                                                                            >  1:239710/1‑140 (MQ=255)                       aaCAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTATGCCGCTTGTTCCGCACATCCGCTGGTGCTGGaa                                                                                                            >  1:39871/1‑140 (MQ=255)                       aaCAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTATGCCGCTTGTTCCGCACATCCGCTGGTGCTGGaa                                                                                                            >  1:136085/1‑140 (MQ=255)                       aaCAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTATGCCGCTTGTTCCGCACATCCGCTGGTGCTGGaa                                                                                                            >  2:590965/1‑140 (MQ=255)                       aaCAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTATGCCGCTTGTTCCGCACATCCGCTGGTGCTGGaa                                                                                                            >  2:444492/1‑140 (MQ=255)                         cAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTATGCCGCTTGTTCCGCACATCCGCTGGTGCTGGAAGc                                                                                                          >  1:41136/1‑140 (MQ=255)                           aaaCGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTATGCCGCTTGTTCCGCACATCCGCTGGTGCTGGAAGCTg                                                                                                        <  2:444862/140‑1 (MQ=255)                            aaCGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTATGCCGCTTGTTCCGCACATCCGCTGGTGCTGGAAGCTGc                                                                                                       >  2:226421/1‑140 (MQ=255)                              cGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTATGCCGCTTGTTCCGCACATCCGCTGGTGCTGGAAGCTGCaa                                                                                                     <  1:771568/140‑1 (MQ=255)                                 aaGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTATGCCGCTTGTTCCGCACATCCGCTGGTGCTGGAAGCTGCAATcc                                                                                                  >  2:1391259/1‑140 (MQ=255)                                   gATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTATGCCGCTTGTTCCGCACATCCGCTGGTGCTGGAAGCTGCAATCCGc                                                                                                <  1:994885/140‑1 (MQ=255)                                   gATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTATGCCGCTTGTTCCGCACATCCGCTGGTGCTGGAAGCTGCAATCCGc                                                                                                <  1:632821/140‑1 (MQ=255)                                    aTTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTATGCCGCTTGTTCCGCACATCCGCTGGTGCTGGAAGCTGCAATCCGc                                                                                                >  2:1126592/1‑139 (MQ=255)                                    aTTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTATGCCGCTTGTTCCGCACATCCGCTGGTGCTGGAAGCTGCAATCCGc                                                                                                <  1:1126592/139‑1 (MQ=255)                                        atatCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTATGCCGCTTGTTCCGCACATCCGCTGGTGCTGGAAGCTGCAATCCGCTACGc                                                                                           <  2:757785/140‑1 (MQ=255)                                        atatCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTATGCCGCTTGTTCCGCACATCCGCTGGTGCTGGAAGCTGCAATCCGCTACGc                                                                                           >  1:208750/1‑140 (MQ=255)                                        atatCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTATGCCGCTTGTTCCGCACATCCGCTGGTGCTGGAAGCTGCAATCCGCTACGc                                                                                           <  1:590965/140‑1 (MQ=255)                                             gCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTATGCCGCTTGTTCCGCACATCCGCTGGTGCTGGAAGCTGCAATCCGCTACGCCAGTg                                                                                      >  1:503118/1‑140 (MQ=255)                                              cAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTATGCCGCTTGTTCCGCACATCCGCTGGTGCTGGAAGCTGCAATCCGCTACGCCAGTGc                                                                                     >  2:1111529/1‑140 (MQ=255)                                              cAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTATGCCGCTTGTTCCGCACATCCGCTGGTGCTGGAAGCTGCAATCCGCTACGCCAGTGc                                                                                     >  1:893556/1‑140 (MQ=255)                                              cAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTATGCCGCTTGTTCCGCACATCCGCTGGTGCTGGAAGCTGCAATCCGCTACGCCAGTGc                                                                                     <  1:1088958/140‑1 (MQ=255)                                                   aTCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTATGCCGCTTGTTCCGCACATCCGCTGGTGCTGGAAGCTGCAATCCGCTACGCCAGTGcaaacc                                                                                >  2:995123/1‑140 (MQ=255)                                                    tCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTATGCCGCTTGTTCCGCACATCCGCTGGTGCTGGAAGCTGCAATCCGCTACGCCAGTGcaaacca                                                                               <  2:663166/140‑1 (MQ=255)                                                        aaCTGGAGAGGAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTATGCCGCTTGTTCCGCACATCCGCTGGTGCTGGAAGCTGCAATCCGCTACGCCAGTGCAAACCAAACg                                                                           >  2:234068/1‑140 (MQ=255)                                                         aCTGGAGAGGAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTATGCCGCTTGTTCCGCACATCCGCTGGTGCTGGAAGCTGCAATCCGCTACGCCAGTGCAAACCAAACGc                                                                          <  2:454086/140‑1 (MQ=255)                                                           tGGAGAGGAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTATGCCGCTTGTTCCGCACATCCGCTGGTGCTGGAAGCTGCAATCCGCTACGCCAGTGCAAACCAAACGCCg                                                                        <  2:1085573/140‑1 (MQ=255)                                                             gagaGGAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTATGCCGCTTGTTCCGCACATCCGCTGGTGCTGGAAGCTGCAATCCGCTACGCCAGTGCAAACCAAACGCCGtt                                                                      >  1:835082/1‑140 (MQ=255)                                                             gagaGGAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTATGCCGCTTGTTCCGCACATCCGCTGGTGCTGGAAGCTGCAATCCGCTACGCCAGTGCAAACCAAACGCCGtt                                                                      >  2:448414/1‑140 (MQ=255)                                                              agagGAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTATGCCGCTTGTTCCGCACATCCGCTGGTGCTGGAAGCTGCAATCCGCTACGCCAGTGCAAACCAAACGCCGTTa                                                                     <  2:239710/140‑1 (MQ=255)                                                              agagGAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTATGCCGCTTGTTCCGCACATCCGCTGGTGCTGGAAGCTGCAATCCGCTACGCCAGTGCAAACCAAACGCCGTTa                                                                     <  2:906936/140‑1 (MQ=255)                                                              agagGAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTATGCCGCTTGTTCCGCACATCCGCTGGTGCTGGAAGCTGCAATCCGCTACGCCAGTGCAAACCAAACGCCGTTa                                                                     <  1:226421/140‑1 (MQ=255)                                                              agagGAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTATGCCGCTTGTTCCGCACATCCGCTGGTGCTGGAAGCTGCAATCCGCTACGCCAGTGCAAACCAAACGCCGTTa                                                                     <  1:1391259/140‑1 (MQ=255)                                                                  gAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTATGCCGCTTGTTCCGCACATCCGCTGGTGCTGGAAGCTGCAATCCGCTACGCCAGTGcaaaccaaa                                                                             >  1:1374696/1‑128 (MQ=255)                                                                  gAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTATGCCGCTTGTTCCGCACATCCGCTGGTGCTGGAAGCTGCAATCCGCTACGCCAGTGcaaaccaaa                                                                             <  2:1374696/128‑1 (MQ=255)                                                                  gAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTATGCCGCTTGTTCCGCACATCCGCTGGTGCTGGAAGCTGCAATCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGa                                                                 >  1:105854/1‑140 (MQ=255)                                                                  gAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTATGCCGCTTGTTCCGCACATCCGCTGGTGCTGGAAGCTGCAATCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGa                                                                 >  2:1146856/1‑140 (MQ=255)                                                                  gAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTATGCCGCTTGTTCCGCACATCCGCTGGTGCTGGAAGCTGCAATCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGa                                                                 >  2:1170865/1‑140 (MQ=255)                                                                   aaaGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTATGCCGCTTGTTCCGCACATCCGCTGGTGCTGGAAGCTGCAATCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGAt                                                                <  2:1329826/140‑1 (MQ=255)                                                                    aaGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTATGCCGCTTGTTCCGCACATCCGCTGGTGCTGGAAGCTGCAATCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGAtt                                                               <  1:1211950/140‑1 (MQ=255)                                                                       tGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTATGCCGCTTGTTCCGCACATCCGCTGGTGCTGGAAGCTGCAATCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGaa                                                            >  1:366755/1‑140 (MQ=255)                                                                        gAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTATGCCGCTTGTTCCGCACATCCGCTGGTGCTGGAAGCTGCAATCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAg                                                           <  1:1111529/140‑1 (MQ=255)                                                                        gAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTATGCCGCTTGTTCCGCACATCCGCTGGTGCTGGAAGCTGCAATCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAg                                                           <  1:515756/140‑1 (MQ=255)                                                                         aaaCATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTATGCCGCTTGTTCCGCACATCCGCTGGTGCTGGAAGCTGCAATCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGc                                                          <  1:1058696/140‑1 (MQ=255)                                                                         aaaCATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTATGCCGCTTGTTCCGCACATCCGCTGGTGCTGGAAGCTGCAATCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGc                                                          <  2:208750/140‑1 (MQ=255)                                                                         aaaCATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTATGCCGCTTGTTCCGCACATCCGCTGGTGCTGGAAGCTGCAATCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGc                                                          <  2:835082/140‑1 (MQ=255)                                                                         aaaCATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTATGCCGCTTGTTCCGCACATCCGCTGGTGCTGGAAGCTGCAATCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGc                                                          <  2:41136/140‑1 (MQ=255)                                                                         aaaCATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTATGCCGCTTGTTCCGCACATCCGCTGGTGCTGGAAGCTGCAATCCGCTACGCCAGTACAAACCAAACGCCGTTACTGATTGAAGc                                                          <  1:995123/140‑1 (MQ=255)                                                                            cATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTATGCCGCTTGTTCCGCACATCCGCTGGTGCTGGAAGCTGCAATCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAAc                                                       >  1:794/1‑140 (MQ=255)                                                                             aTCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTATGCCGCTTGTTCCGCACATCCGCTGGTGCTGGAAGCTGCAATCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAAcc                                                      <  2:893556/140‑1 (MQ=255)                                                                             aTCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTATGCCGCTTGTTCCGCACATCCGCTGGTGCTGGAAGCTGCAATCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAAcc                                                      <  1:448414/140‑1 (MQ=255)                                                                                 gACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTATGCCGCTTGTTCCGCACATCCGCTGGTGCTGGAAGCTGCAATCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAg                                                           <  2:1273828/131‑1 (MQ=255)                                                                                 gACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTATGCCGCTTGTTCCGCACATCCGCTGGTGCTGGAAGCTGCAATCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAg                                                           >  1:1273828/1‑131 (MQ=255)                                                                                 gACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTATGCCGCTTGTTCCGCACATCCGCTGGTGCTGGAAGCTGCAATCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCa                                                  <  1:1146856/140‑1 (MQ=255)                                                                                 gACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTATGCCGCTTGTTCCGCACATCCGCTGGTGCTGGAAGCTGCAATCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCa                                                  >  1:741919/1‑140 (MQ=255)                                                                                 gACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTATGCCGCTTGTTCCGCACATCCGCTGGTGCTGGAAGCTGCAATCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCa                                                  >  1:831843/1‑140 (MQ=255)                                                                                  aCAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTATGCCGCTTGTTCCGCACATCCGCTGGTGCTGGAAGCTGCAATCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCaa                                                 <  2:774452/140‑1 (MQ=255)                                                                                  aCAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTATGCCGCTTGTTCCGCACATCCGCTGGTGCTGGAAGCTGCAATCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCaa                                                 <  2:741919/140‑1 (MQ=255)                                                                                      aaaTGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTATGCCGCTTGTTCCGCACATCCGCTGGTGCTGGAAGCTGCAATCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAg                                             <  2:136085/140‑1 (MQ=255)                                                                                       aaTGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTATGCCGCTTGTTCCGCACATCCGCTGGTGCTGGAAGCTGCAATCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAgg                                            <  2:366755/140‑1 (MQ=255)                                                                                         tGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTATGCCGCTTGTTCCGCACATCCGCTGGTGCTGGAAGCTGCAATCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTa                                          >  1:848113/1‑140 (MQ=255)                                                                                            tGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTATGCCGCTTGTTCCGCACATCCGCTGGTGCTGGAAGCTGCAATCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGAc                                       >  2:976103/1‑140 (MQ=255)                                                                                                   gCACAAAGCGGGCAAAACAAATGGAATTTATGCCGCTTGTTCCGCACATCCGCTGGTGCTGGAAGCTGCAATCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTcg                                <  1:444492/140‑1 (MQ=255)                                                                                                    cacaAAGCGGGCAAAACAAATGGAATTTATGCCGCTTGTTCCGCACATCCGCTGGTGCTGGAAGCTGCAATCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTcgg                               <  2:105854/140‑1 (MQ=255)                                                                                                     acaAAGCGGGCAAAACAAATGGAATTTATGCCGCTTGTTCCGCACATCCGCTGGTGCTGGAAGCTGCAATCCGCTACGCCAGTGCAGACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTcggc                              >  2:414619/1‑140 (MQ=255)                                                                                                     acaAAGCGGGCAAAACAAATGGAATTTATGCCGCTTGTTCCGCACATCCGCTGGTGCTGGAAGCTGCAATCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGAc                                       >  1:925472/1‑131 (MQ=255)                                                                                                     acaAAGCGGGCAAAACAAATGGAATTTATGCCGCTTGTTCCGCACATCCGCTGGTGCTGGAAGCTGCAATCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGAc                                       <  2:925472/131‑1 (MQ=255)                                                                                                     acaAAGCGGGCAAAACAAATGGAATTTATGCCGCTTGTTCCGCACATCCGCTGGTGCTGGAAGCTGCAATCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTcggc                              <  2:831843/140‑1 (MQ=255)                                                                                                              gCAAAACAAATGGAATTTATGCCGCTTGTTCCGCACATCCGCTGGTGCTGGAAGCTGCAATCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTcg                                <  1:23735/129‑1 (MQ=255)                                                                                                              gCAAAACAAATGGAATTTATGCCGCTTGTTCCGCACATCCGCTGGTGCTGGAAGCTGCAATCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTcg                                >  2:23735/1‑129 (MQ=255)                                                                                                                        tGGAATTTATGCCGCTTGTTCCGCACATCCGCTGGTGCTGGAAGCTGCAATCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGAcgc           >  2:412973/1‑140 (MQ=255)                                                                                                                             tttATGCCGCTTGTTCCGCACATCCGCTGGTGCTGGAAGCTGCAATCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGAcgcccgcc      >  1:102567/1‑140 (MQ=255)                                                                                                                              ttATGCCGCTTGTTCCGCACATCCGCTGGTGCTGGAAGCTGCAATCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCccgccg     >  1:1364549/1‑140 (MQ=255)                                                                                                                                 tGCCGCTTGTTCCGCACATCCGCTGGTGCTGGAAGCTGCAATCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGAtt  >  1:563955/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                       ACCATGACGCAGTATCAACTGAAACAAAACGAAAGATTAATATCGCAGTAATCTGAACTGGAGAGGAAAGTGAAACATCTGACAGAAATGGTGAGACAGCACAAAGCGGGCAAAACAAATGGAATTTATGCCGTTTGTTCCGCACATCCGCTGGTGCTGGAAGCTGCAATCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATT  >  NC_000913/3278845‑3279112

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑