Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,401,949	G→A	R462C (CGT→TGT)	csrD ←	regulator of CsrB and CsrC decay

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,401,949	0	G	A	100.0%	227.4 / NA	63	R462C (CGT→TGT)	csrD	regulator of CsrB and CsrC decay
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (33/30);  total (33/30)

TTCACATTGCATTAACGTATCGCGCAGCCAACGCTGAAAACGCGGGCGAATCAGCGACTCAACGGTAACCTGAATCGCCAGATTTTCCTCTGGCCAGTAACGCAATAGTGGAATAAGACGGCTGATTTGCAGACGGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTACCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAAC  >  NC_000913/3401815‑3402075                                                                                                                                       |                                                                                                                               ttCACATTGCATTAACGTATCGCGCAGCCAACGCTGAAAACGCGGGCGAATCAGCGACTCAACGGTAACCTGAATCGCCAGATTTTCCTCTGGCCAGTAACGCAATAGTGGAATAAGACGGCTGATTTGCAGACAGTCAt                                                                                                                           <  2:666837/140‑1 (MQ=255)  tCACATTGCATTAACGTATCGCGCAGCCAACGCTGAAAACGCGGGCGAATCAGCGACTCAACGGTAACCTGAATCGCCAGATTTTCCTCTGGCCAGTAACGCAATAGTGGAATAAGACGGCTGATTTGCAGACAGTCATa                                                                                                                          <  2:1385008/140‑1 (MQ=255)      aTTGCATTAACGTATCGCGCAGCCAACGCTGAAAACGCGGGCGAATCAGCGACTCAACGGTAACCTGAATCGCCAGATTTTCCTCTGGCCAGTAACGCAATAGTGGAATAAGACGGCTGATTTGCAGACAGTCATActct                                                                                                                      <  1:87241/140‑1 (MQ=255)        tGCATTAACGTATCGCGCAGCCAACGCTGAAAACGCGGGCGAATCAGCGACTCAACGGTAACCTGAATCGCCAGATTTTCCTCTGGCCAGTAACGCAATAGTGGAATAAGACGGCTGATTTGCAGACAGTCATACTCTTc                                                                                                                    >  2:565807/1‑140 (MQ=255)          cATTAACGTATCGCGCAGCCAACGCTGAAAACGCGGGCGAATCAGCGACTCAACGGTAACCTGAATCGCCAGATTTTCCTCTGGCCAGTAACGCAATAGTGGAATAAGACGGCTGATTTGCAGACAGTCATACTCTTCCg                                                                                                                  >  1:501276/1‑140 (MQ=255)          cATTAACGTATCGCGCAGCCAACGCTGAAAACGCGGGCGAATCAGCGACTCAACGGTAACCTGAATCGCCAGATTTTCCTCTGGCCAGTAACGCAATAGTGGAATAAGACGGCTGATTTGCAGACAGTCATACTCTTCCg                                                                                                                  >  2:92929/1‑140 (MQ=255)             tAACGTATCGCGCAGCCAACGCTGAAAACGCGGGCGAATCAGCGACTCAACGGTAACCTGAATCGCCAGATTTTCCTCTGGCCAGTAACGCAATAGTGGAATAAGACGGCTGATTTGCAGACAGTCATACTCTTCCGATa                                                                                                               >  2:510196/1‑140 (MQ=255)              aaCGTATCGCGCAGCCAACGCTGAAAACGCGGGCGAATCAGCGACTCAACGGTAACCTGAATCGCCAGATTTTCCTCTGGCCAGTAACGCAATAGTGGAATAAGACGGCTGATTTGCAGACAGTCATACTCTTCCGATaa                                                                                                              <  1:1175309/140‑1 (MQ=255)                  tATCGCGCAGCCAACGCTGAAAACGCGGGCGAATCAGCGACTCAACGGTAACCTGAATCGCCAGATTTTCCTCTGGCCAGTAACGCAATAGTGGAATAAGACGGCTGATTTGCAGACAGTCATACTCTTCCGATAAGCCa                                                                                                          >  1:955121/1‑140 (MQ=255)                  tATCGCGCAGCCAACGCTGAAAACGCGGGCGAATCAGCGACTCAACGGTAACCTGAATCGCCAGATTTTCCTCTGGCCAGTAACGCAATAGTGGAATAAGACGGCTGATTTGCAGACAGTCATACTCTTCCGATAAGCCa                                                                                                          <  1:486552/140‑1 (MQ=255)                  tATCGCGCAGCCAACGCTGAAAACGCGGGCGAATCAGCGACTCAACGGTAACCTGAATCGCCAGATTTTCCTCTGGCCAGTAACGCAATAGTGGAATAAGACGGCTGATTTGCAGACAGTCATACTCTTCCGATAAGCCa                                                                                                          <  1:510196/140‑1 (MQ=255)                  tATCGCGCAGCCAACGCTGAAAACGCGGGCGAATCAGCGACTCAACGGTAACCTGAATCGCCAGATTTTCCTCTGGCCAGTAACGCAATAGTGGAATAAGACGGCTGATTTGCAGACAGTCATACTCTTCCGATAAGCCa                                                                                                          >  1:792594/1‑140 (MQ=255)                    tCGCGCAGCCAACGCTGAAAACGCGGGCGAATCAGCGACTCAACGGTAACCTGAATCGCCAGATTTTCCTCTGGCCAGTAACGCAATAGTGGAATAAGACGGCTGATTTGCAGACAGTCATACTCTTCCGATAAGCCaaa                                                                                                        >  1:1333233/1‑140 (MQ=255)                    tCGCGCAGCCAACGCTGAAAACGCGGGCGAATCAGCGACTCAACGGTAACCTGAATCGCCAGATTTTCCTCTGGCCAGTAACGCAATAGTGGAATAAGACGGCTGATTTGCAGACAGTCATACTCTTCCGATAAGCCaaa                                                                                                        >  1:1076056/1‑140 (MQ=255)                     cgcgCAGCCAACGCTGAAAACGCGGGCGAATCAGCGACTCAACGGTAACCTGAATCGCCAGATTTTCCTCTGGCCAGTAACGCAATAGTGGAATAAGACGGCTGATTTGCAGACAGTCATACTCTTCCGATAAGCCAAAc                                                                                                       >  1:1349497/1‑140 (MQ=255)                      gcgcAGCCAACGCTGAAAACGCGGGCGAATCAGCGACTCAACGGTAACCTGAATCGCCAGATTTTCCTCTGGCCAGTAACGCAATAGTGGAATAAGACGGCTGATTTGCAGACAGTCATACTCTTCCGATAAGCCAAACt                                                                                                      >  1:1330755/1‑140 (MQ=255)                       cgcAGCCAACGCTGAAAACGCGGGCGAATCAGCGACTCAACGGTAACCTGAATCGCCAGATTTTCCTCTGGCCAGTAACGCAATAGTGGAATAAGACGGCTGATTTGCAGACAGTCATACTCTTCCGATAAGCCAAACTg                                                                                                     >  1:87270/1‑140 (MQ=255)                         cAGCCAACGCTGAAAACGCGGGCGAATCAGCGACTCAACGGTAACCTGAATCGCCAGATTTTCCTCTGGCCAGTAACGCAATAGTGGAATAAGACGGCTGATTTGCAGACAGTCATACTCTTCCGATAAGCCa                                                                                                          >  1:924450/1‑133 (MQ=255)                         cAGCCAACGCTGAAAACGCGGGCGAATCAGCGACTCAACGGTAACCTGAATCGCCAGATTTTCCTCTGGCCAGTAACGCAATAGTGGAATAAGACGGCTGATTTGCAGACAGTCATACTCTTCCGATAAGCCa                                                                                                          <  2:924450/133‑1 (MQ=255)                          aGCCAACGCTGAAAACGCGGGCGAATCAGCGACTCAACGGTAACCTGAATCGCCAGATTTTCCTCTGGCCAGTAACGCAATAGTGGAATAAGACGGCTGATTTGCAGACAGTCATACTCTTCCGATAAGCCAAACTGCaa                                                                                                  >  2:156105/1‑140 (MQ=255)                               aCGCTGAAAACGCGGGCGAATCAGCGACTCAACGGTAACCTGAATCGCCAGATTTTCCTCTGGCCAGTAACGCAATAGTGGAATAAGACGGCTGATTTGCAGACAGTCATACTCTTCCGATAAGCCAAACTGCAAGACCa                                                                                             <  2:1333233/140‑1 (MQ=255)                                cGCTGAAAACGCGGGCGAATCAGCGACTCAACGGTAACCTGAATCGCCAGATTTTCCTCTGGCCAGTAACGCAATAGTGGAATAAGACGGCTGATTTGCAGACAGTCATACTCTTCCGATAAGCCAAACTGCAAGACCAt                                                                                            <  2:1076056/140‑1 (MQ=255)                                cGCTGAAAACGCGGGCGAATCAGCGACTCAACGGTAACCTGAATCGCCAGATTTTCCTCTGGCCAGTAACGCAATAGTGGAATAAGACGGCTGATTTGCAGACAGTCATACTCTTCCGATAAGCCAAACTGCAAGACCAt                                                                                            >  2:310696/1‑140 (MQ=255)                                cGCTGAAAACGCGGGCGAATCAGCGACTCAACGGTAACCTGAATCGCCAGATTTTCCTCTGGCCAGTAACGCAATAGTGGAATAAGACGGCTGATTTGCAGACAGTCATACTCTTCCGATAAGCCAAACTGCAAGACCAt                                                                                            <  2:1330755/140‑1 (MQ=255)                                         cgcgGGCGAATCAGCGACTCAACGGTAACCTGAATCGCCAGATTTTCCTCTGGCCAGTAACGCAATAGTGGAATAAGACGGCTGATTTGCAGACAGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCAtata                                                                                   <  1:310696/140‑1 (MQ=255)                                           cgGGCGAATCAGCGACTCAACGGTAACCTGAATCGCCAGATTTTCCTCTGGCCAGTAACGCAATAGTGGAATAAGACGGCTGATTTGCAGACAGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACt                                                                                 >  2:735465/1‑140 (MQ=255)                                                     aGCGACTCAACGGTAACCTGAATCGCCAGATTTTCCTCTGGCCAGTAACGCAATAGTGGAATAAGACGGCTGATTTGCAGACAGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCt                                                                       <  1:318833/140‑1 (MQ=255)                                                        gACTCAACGGTAACCTGAATCGCCAGATTTTCCTCTGGCCAGTAACGCAATAGTGGAATAAGACGGCTGATTTGCAGACAGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAAc                                                                    <  1:1347111/140‑1 (MQ=255)                                                        gACTCAACGGTAACCTGAATCGCCAGATTTTCCTCTGGCCAGTAACGCAATAGTGGAATAAGACGGCTGATTTGCAGACAGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAAc                                                                    <  1:977023/140‑1 (MQ=255)                                                              aCGGTAACCTGAATCGCCAGATTTTCCTCTGGCCAGTAACGCAATAGTGGAATAAGACGGCTGATTTGCAGACAGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTc                                                              <  1:735465/140‑1 (MQ=255)                                                               cGGTAACCTGAATCGCCAGATTTTCCTCTGGCCAGTAACGCAATAGTGGAATAAGACGGCTGATTTGCAGACAGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCa                                                             <  2:87270/140‑1 (MQ=255)                                                                       tGAATCGCCAGATTTTCCTCTGGCCAGTAGCGCAATAGTGGAATAAGACGGCTGATTTGCAGACAGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTACCATc                                                     >  1:219284/1‑140 (MQ=255)                                                                       tGAATCGCCAGATTTTCCTCTGGCCAGTAACGCAATAGTGGAATAAGACGGCTGATTTGCAGACAGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTa                                                          <  1:734267/135‑1 (MQ=255)                                                                       tGAATCGCCAGATTTTCCTCTGGCCAGTAACGCAATAGTGGAATAAGACGGCTGATTTGCAGACAGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTa                                                          >  2:734267/1‑135 (MQ=255)                                                                        gAATCGCCAGATTTTCCTCTGGCCAGTAACGCAATAGTGGAATAAGACGGCTGATTTGCAGACAGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTACCATCg                                                    <  2:501276/140‑1 (MQ=255)                                                                         aaTCGCCAGATTTTCCTCTGGCCAGTAACGCAATAGTGGAATAAGACGGCTGATTTGCAGACAGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCtt                                                               >  2:1194764/1‑128 (MQ=255)                                                                         aaTCGCCAGATTTTCCTCTGGCCAGTAACGCAATAGTGGAATAAGACGGCTGATTTGCAGACAGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCtt                                                               <  1:1194764/128‑1 (MQ=255)                                                                         aaTCGCCAGATTTTCCTCTGGCCAGTAACGCAATAGTGGAATAAGACGGCTGATTTGCAGACAGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTACCATCGa                                                   >  2:1030060/1‑140 (MQ=255)                                                                              ccAGATTTTCCTCTGGCCAGTAACGCAATAGTGGAATAAGACGGCTGATTTGCAGACAGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTACCATCGAAGATg                                              <  1:92929/140‑1 (MQ=255)                                                                              ccAGATTTTCCTCTGGCCAGTAACGCAATAGTGGAATAAGACGGCTGATTTGCAGACAGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTACCATCGAAGATg                                              <  1:335544/140‑1 (MQ=255)                                                                              ccAGATTTTCCTCTGGCCAGTAACGCAATAGTGGAATAAGACGGCTGATTTGCAGACAGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTACCATCGAAGATg                                              <  2:1349497/140‑1 (MQ=255)                                                                              ccAGATTTTCCTCTGGCCAGTAACGCAATAGTGGAATAAGACGGCTGATTTGCAGACAGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTACCATCGAAGATg                                              >  2:1362030/1‑140 (MQ=255)                                                                              ccAGATTTTCCTCTGGCCAGTAACGCAATAGTGGAATAAGACGGCTGATTTGCAGACAGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTACCATCGAAGATg                                              <  1:156105/140‑1 (MQ=255)                                                                                aGATTTTCCTCTGGCCAGTAACGCAATAGTGGAATAAGACGGCTGATTTGCAGACAGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTACCATCGAAGATGCg                                            <  2:792594/140‑1 (MQ=255)                                                                                    tttCCTCTGGCCAGTAACGCAATAGTGGAATAAGACGGCTGATTTGCAGACAGTCATACTCTTCCGATAAGCCAAACGACAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTACCATCGAAGATGCGGcac                                        <  2:1248542/140‑1 (MQ=255)                                                                                             gCCAGTAACGCAATAGTGGAATAAGACGGCTGATTTGCAGACAGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTACCATCGAAGATGCGGCACATGAGTTcg                               >  2:601447/1‑140 (MQ=255)                                                                                             gCCAGTAACGCAATAGTGGAATAAGACGGCTGATTTGCAGACAGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTACCATCGAAGATGCGGCACATGAGTTcg                               <  1:1113242/140‑1 (MQ=255)                                                                                                   aaCGCAATAGTGGAATAAGACGGCTGATTTGCAGACAGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTACCATCGAAGATGCGGCACATGAGTTCGCgatga                         <  1:1030060/140‑1 (MQ=255)                                                                                                       cAATAGTGGAATAAGACGGCTGATTTGCAGACAGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTACCATCGAAGATGCGGCACATGAGTTCGCGATGATGaa                     >  1:1030724/1‑140 (MQ=255)                                                                                                       cAATAGTGGAATAAGACGGCTGATTTGCAGACAGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTACCATCGAAGATGCGGCACATGAGTTCGCGATGATGaa                     >  2:1203802/1‑140 (MQ=255)                                                                                                        aaTAGTGGAATAAGACGGCTGATTTGCAGACAGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTACCATCGAAGATGCGGCACATGAGTTCGCGATGATGAAc                    <  2:955121/140‑1 (MQ=255)                                                                                                           aGTGGAATAAGACGGCTGATTTGCAGACAGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTACCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTg                 >  1:196484/1‑140 (MQ=255)                                                                                                            gTGGAATAAGACGGCTGATTTGCAGACAGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTACCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGa                >  2:498397/1‑140 (MQ=255)                                                                                                            gTGGAATAAGACGGCTGATTTGCAGACAGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTACCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGa                >  2:705049/1‑140 (MQ=255)                                                                                                             tGGAATAAGACGGCTGATTTGCAGACAGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTACCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGAc               >  1:295649/1‑140 (MQ=255)                                                                                                             tGGAATAAGACGGCTGATTTGCAGACAGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTACCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGAc               >  2:821335/1‑140 (MQ=255)                                                                                                                aaTAAGACGGCTGATTTGCAGACAGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTACCATCGAAGATGCGGCACATGAGTTCGCGatgatg                       <  2:140453/129‑1 (MQ=255)                                                                                                                aaTAAGACGGCTGATTTGCAGACAGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTACCATCGAAGATGCGGCACATGAGTTCGCGatgatg                       >  1:140453/1‑129 (MQ=255)                                                                                                                    aGACGGCTGATTTGCAGACAGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTACCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTcgcg        >  1:1295374/1‑140 (MQ=255)                                                                                                                    aGACGGCTGATTTGCAGACAGTCATACTCTTCCGATAAGCCAAACCGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTACCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCt             <  2:167126/135‑1 (MQ=255)                                                                                                                    aGACGGCTGATTTGCAGACAGTCATACTCTTCCGATAAGCCAAACCGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTACCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCt             >  1:167126/1‑135 (MQ=255)                                                                                                                       cGGCTGATTTGCAGACAGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTACCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGt     >  2:121757/1‑140 (MQ=255)                                                                                                                       cGGCTGATTTGCAGACAGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTACCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGt     >  1:1071100/1‑140 (MQ=255)                                                                                                                          cTGATTTGCAGACAGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTACCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAAc  >  1:810442/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                               TTCACATTGCATTAACGTATCGCGCAGCCAACGCTGAAAACGCGGGCGAATCAGCGACTCAACGGTAACCTGAATCGCCAGATTTTCCTCTGGCCAGTAACGCAATAGTGGAATAAGACGGCTGATTTGCAGACGGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTACCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAAC  >  NC_000913/3401815‑3402075

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑