Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,419,129	C→T	pseudogene (88/1025 nt)	yhdW →	putative ABC transporter periplasmic binding protein YhdW

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,419,129	0	C	T	100.0%	332.5 / NA	93	pseudogene (88/1025 nt)	yhdW	putative ABC transporter periplasmic binding protein YhdW
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (40/53);  total (40/53)

TTTCCCAAACGTCCTATAACGATAATTTTCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGCCGTTCAGAAAAAAGGTTTTGTGCAATGCGGGATCAGTGATGGATTACCTGGGTTCTCTTATGCCGATGCTGACGGTAAGTTTTCAGGTATTGATGTTGATATTTGTCGTGGTGTTGCCGCTGCTGTATTTGGTG  >  NC_000913/3418995‑3419263                                                                                                                                       |                                                                                                                                       tttCCCAAACGTCCTATAACGATAATTTTCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGTCGTTc                                                                                                                                   >  2:1203897/1‑140 (MQ=255) tttCCCAAACGTCCTATAACGATAATTTTCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGTCGTTc                                                                                                                                   >  2:1148002/1‑140 (MQ=255) tttCCCAAACGTCCTATAACGATAATTTTCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGTCGTTc                                                                                                                                   <  2:1105624/140‑1 (MQ=255) tttCCCAAACGTCCTATAACGATAATTTTCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGTCGTTc                                                                                                                                   >  2:694596/1‑140 (MQ=255)   tCCCAAACGTCCTATAACGATAATTTTCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGTCGTTCAg                                                                                                                                 >  1:276942/1‑140 (MQ=255)         aCGTCCTATAACGATAATTTTCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGTCGTTCAGaaaaa                                                                                                                            >  2:557719/1‑139 (MQ=255)         aCGTCCTATAACGATAATTTTCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGTCGTTCAGaaaaa                                                                                                                            <  1:557719/139‑1 (MQ=255)           gTCCTATAACGATAATTTTCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGTCGTTCAGAAAAAAgg                                                                                                                         >  2:124894/1‑140 (MQ=255)              cTATAACGATAATTTTCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGTCGTTCAGAAAAAAGGttt                                                                                                                      >  2:205705/1‑140 (MQ=255)              cTATAACGATAATTTTCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGTCGTTCAGAAAAAAGGttt                                                                                                                      <  2:1101959/140‑1 (MQ=255)              cTATAACGATAATTTTCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGTCGTTCAGAAAAAAGGttt                                                                                                                      >  2:1091835/1‑140 (MQ=255)              cTATAACGATAATTTTCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGC‑TGATGTCGTTCAGAAAAAAGGtttt                                                                                                                     >  2:275368/1‑140 (MQ=255)                 taACGATAATTTTCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGTCGTTCAGAAAAAAGGTTTtgt                                                                                                                   >  2:180592/1‑140 (MQ=255)                  aaCGATAATTTTCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGTCGTTCAGAAAAAAGGTTTtgtg                                                                                                                  <  1:1385994/140‑1 (MQ=255)                  aaCGATAATTTTCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGTCGTTCAGAAAAAAGGTTTtgtg                                                                                                                  <  2:1200531/140‑1 (MQ=255)                  aaCGATAATTTTCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGTCGTTCAGAAAAAAGGTTTtgtg                                                                                                                  <  2:54416/140‑1 (MQ=255)                   aCGATAATTTTCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGTCGTTCAGAAAAAAGGTTTTGTGc                                                                                                                 <  1:201205/140‑1 (MQ=255)                     gATAATTTTCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGTCGTTCAGAAAAAAGGTTTtgtg                                                                                                                  >  2:861873/1‑137 (MQ=255)                     gATAATTTTCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGTCGTTCAGAAAAAAGGTTTtgtg                                                                                                                  <  1:861873/137‑1 (MQ=255)                     gATAATTTTCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGTCGTTCAGAAAAAAGGTTTTGTGCaa                                                                                                               <  2:1143720/140‑1 (MQ=255)                      aTAATTTTCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGTCGTTCAGAAAAAAGGTTTTGTGCAAt                                                                                                              >  1:883324/1‑140 (MQ=255)                      aTAATTTTCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGTCGTTCAGAAAAAAGGTTTTGTGCAAt                                                                                                              >  2:509037/1‑140 (MQ=255)                      aTAATTTTCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGTCGTTCAGAAAAAAGGTTTTGTGCAAt                                                                                                              <  1:205705/140‑1 (MQ=255)                       tAATTTTCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGTCGTTCAGAAAAAAGGTTTtgtg                                                                                                                  >  2:40412/1‑135 (MQ=255)                       tAATTTTCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGTCGTTCAGAAAAAAGGTTTtgtg                                                                                                                  <  1:40412/135‑1 (MQ=255)                       tAATTTTCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGTCGTTCAGAAAAAAGGTTTTGTGCAATg                                                                                                             <  1:992871/140‑1 (MQ=255)                       tAATTTTCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGTCGTTCAGAAAAAAGGTTTTGTGCAATg                                                                                                             >  1:75526/1‑140 (MQ=255)                       tAATTTTCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGTCGTTCAGAAAAAAGGTTTTGTGCAATg                                                                                                             >  1:1156194/1‑140 (MQ=255)                       tAATTTTCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGTCGTTCAGAAAAAAGGTTTTGTGCAATg                                                                                                             >  1:312349/1‑140 (MQ=255)                       tAATTTTCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGTCGTTCAGAAAAAAGGTTTTGTGCAATg                                                                                                             >  2:181477/1‑140 (MQ=255)                         aTTTTCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGTCGTTCAGAAAAAAGGTTTTGTGCAATGCg                                                                                                           >  2:377737/1‑140 (MQ=255)                             tCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGTCGTTCAGAAAAAAGGTTTTGTGCAATGCGGGAt                                                                                                       <  1:693696/140‑1 (MQ=255)                             tCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGTCGTTCAGAAAAAAGGTTTTGTGCAATGCGGGAt                                                                                                       <  2:1170167/140‑1 (MQ=255)                             tCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGTCGTTCAGAAAAAAGGTTTTGTGCAATGCGGGAt                                                                                                       <  1:181477/140‑1 (MQ=255)                             tCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGTCGTTCAGAAAAAAGGTTTTGTGCAATGCGGGAt                                                                                                       <  2:1247370/140‑1 (MQ=255)                             tCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAACCAGGCGCTGCTGGCGCGACGCTTGATGTCGTTCAGAAAAAAGGTTTTGTGCAATGCGGGAt                                                                                                       <  2:1248696/140‑1 (MQ=255)                               gCCTCACAGGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGTCGTTCAGAAAAAAGGTTTTGTGCAATGCGGGATCa                                                                                                     >  2:682747/1‑140 (MQ=255)                                 cTCACAGGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGTCGTTCAGAAAAAAGGTTTTGTGCAATGCGGGATCAGt                                                                                                   <  2:245191/140‑1 (MQ=255)                                 cTCACAGGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGTCGTTCAGAAAAAAGGTTTTGTGCAATGCGGGATCAGt                                                                                                   <  2:1156194/140‑1 (MQ=255)                                     caGGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGTCGTTCAGAAAAAAGGTTTTGTGCAATGCGGGATCAGTGATg                                                                                               <  2:1306330/140‑1 (MQ=255)                                        gAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGTCGTTCAGAAAAAAGGTTTTGTGCAATGCGGGATCAGTGATGGAt                                                                                            >  1:111681/1‑140 (MQ=255)                                          aGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGTCGTTCAGAAAAAAGGTTTTGTGCAATGCGGGATCAGTGATGGATTa                                                                                          <  2:485051/140‑1 (MQ=255)                                           gCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGTCGTTCAGAAAAAAGGTTTTGTGCAATGCGGGAt                                                                                                       <  2:185743/126‑1 (MQ=255)                                           gCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGTCGTTCAGAAAAAAGGTTTTGTGCAATGCGGGAt                                                                                                       >  1:185743/1‑126 (MQ=255)                                           gCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGTCGTTCAGAAAAAAGGTTTTGTGCAATGCGGGATCAGTGATGGATTAc                                                                                         >  2:270302/1‑140 (MQ=255)                                            cATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGTCGTTCAGAAAAAAGGTTTTGTGCAATGCGGGATCAGTGATGGATTTcc                                                                                        <  2:779148/140‑1 (MQ=255)                                             aTTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGTCGTTCAGAAAAAAGGTTTTGTGCAATGCGGGATCAGTGATGGATTACCt                                                                                       <  1:1091835/140‑1 (MQ=255)                                                aTGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGTCGTTCAGAAAAAAGGTTTTGTGCAATGCGGGATCAGTGATGGATTACCTggg                                                                                    <  2:101317/140‑1 (MQ=255)                                                aTGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGTCGTTCAGAAAAAAGGTTTTGTGCAATGCGGGATCAGTGATGGATTACCTggg                                                                                    <  2:192192/140‑1 (MQ=255)                                                aTGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGTCGTTCAGAAAAAAGGTTTTGTGCAATGCGGGATCAGTGATGGATTACCTggg                                                                                    <  2:1140616/140‑1 (MQ=255)                                                aTGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGTCGTTCAGAAAAAAGGTTTTGTGCAATGCGGGATCAGTGATGGATTACCTggg                                                                                    >  2:746653/1‑140 (MQ=255)                                                aTGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGTCGTTCAGAAAAAAGGTTTTGTGCAATGCGGGATCAGTGATGGATTACCTggg                                                                                    <  2:1049224/140‑1 (MQ=255)                                                aTGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGTCGTTCAGAAAAAAGGTTTTGTGCAATGCGGGATCAGTGATGGATTACCTggg                                                                                    <  2:312487/140‑1 (MQ=255)                                                aTGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGTCGTTCAGAAAAAAGGTTTTGTGCAATGCGGGATCAGTGATGGATTACCTggg                                                                                    <  2:450446/140‑1 (MQ=255)                                                aTGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGTCGTTCAGAAAAAAGGTTTTGTGCAATGCGGGATCAGTGATGGATTACCTggg                                                                                    <  1:270302/140‑1 (MQ=255)                                                      aaGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGTCGTTCAGAAAAAAGGTTTTGTGCAATGCGGGATCAGTGATGGATTACCTGGGTTctct                                                                              >  2:264397/1‑140 (MQ=255)                                                       aGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGTCGTTCAGAAAAAAGGTTTTGTGCAATGCGGGATCAGTGATGGATTACCTGGGTTCTCtt                                                                             >  2:1183540/1‑140 (MQ=255)                                                        gatgatgatAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGTCGTTCAGAAAAAAGGTTTTGTGCAATGCGGGATCAGTGATGGATTACCTGGGTTCTCTTa                                                                            <  2:1300879/140‑1 (MQ=255)                                                           gatgatAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGTCGTTCAGAAAAAAGGTTTTGTGCAATGCGGGATCAGTGATGGATTACCTGGGTTCTCTTATGc                                                                         >  1:476194/1‑140 (MQ=255)                                                            atgatAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGTCGTTCAGAAAAAAGGTTTTGTGCAATGCGGGATCAGTGATGGATTACCTGGGTTCTCTTATGcc                                                                        <  2:883324/140‑1 (MQ=255)                                                                      cacTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGTCGTTCAGAAAAAAGGTTTTGTGCAATGCGGGATCAGTGATGGATTACCTGGGTTCTCTTATGCCGATGCt                                                                  <  2:980041/136‑1 (MQ=255)                                                                      cacTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGTCGTTCAGAAAAAAGGTTTTGTGCAATGCGGGATCAGTGATGGATTACCTGGGTTCTCTTATGCCGATGCt                                                                  >  1:980041/1‑136 (MQ=255)                                                                       acTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGTCGTTCAGAAAAAAGGTTTTGTGCAATGCGGGATCAGTGATGGATTACCTGGGTtctc                                                                               >  2:1168433/1‑122 (MQ=255)                                                                       acTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGTCGTTCAGAAAAAAGGTTTTGTGCAATGCGGGATCAGTGATGGATTACCTGGGTtctc                                                                               <  1:1168433/122‑1 (MQ=255)                                                                       acTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGTCGTTCAGAAAAAAGGTTTTGTGCAATGCGGGATCAGTGATGGATTACCTGGGTTCTCTTATGCCGATGCTGACgg                                                             >  1:1345382/1‑140 (MQ=255)                                                                        cTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGTCGTTCAGAAAAAAGGTTTTGTGCAATGCGGGATCAGTGATGGATTACCTGGGTTCTCTTATGCCGATGCTGACGGt                                                            <  2:1345382/140‑1 (MQ=255)                                                                        cTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGTCGTTCAGAAAAAAGGTTTTGTGCAATGCGGGATCAGTGATGGATTACCTGGGTTCTCTTATGCCGATGCTGACGGt                                                            <  1:126579/140‑1 (MQ=255)                                                                        cTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGTCGTTCAGAAAAAAGGTTTTGTGCAATGCGGGATCAGTGATGGATTACCTGGGTTCTCTTATGCCGATGCTGACGGt                                                            <  2:651758/140‑1 (MQ=255)                                                                          ggCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGTCGTTCAGAAAAAAGGTTTTGTGCAATGCGGGATCAGTGATGGATTACCTGGGTTCTCTTATGCCGATGCTGACGGTaa                                                          <  2:276942/140‑1 (MQ=255)                                                                           gCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGTCGTTCAGAAAAAAGGTTTTGTGCAATGCGGGATCAGTGATGGATTACCTGGGTTCTCTTATGCCGATGCTGACGGTAAg                                                         <  2:312349/140‑1 (MQ=255)                                                                           gCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGTCGTTCAGAAAAAAGGTTTTGTGCAATGCGGGATCAGTGATGGATTACCTGGGTTCTCTTATGCCGATGCTGACGGTAAg                                                         >  1:1233848/1‑140 (MQ=255)                                                                                cgccAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGTCGTTCAGAAAAAAGGTTTTGTGCAATGCGGGATCAGTGATGGATTACCTGGGTTCTCTTATGCCGATGCTGACGGTAAGTTTTc                                                    <  2:337256/140‑1 (MQ=255)                                                                                cgccAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGTCGTTCAGAAAAAAGGTTTTGTGCAATGCGGGATCAGTGATGGATTACCTGGGTTCTCTTATGCCGATGCTGACGGTAAGTTTTc                                                    >  2:721487/1‑140 (MQ=255)                                                                                cgccAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGTCGTTCAGAAAAAAGGTTTTGTGCAATGCGGGATCAGTGATGGATTACCTGGGTTCTCTTATGCCGATGCTGACGGTAAGTTTTc                                                    >  1:335582/1‑140 (MQ=255)                                                                                     gCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGTCGTTCAGAAAAAAGGTTTTGTGCAATGCGGGATCAGTGATGGATTACCTGGGTTCTCTTATGCCGATGCTGACGGTAAGTTTTCAGGTa                                               <  2:1168220/140‑1 (MQ=255)                                                                                     gCGTGCTGCTTGCCGTTGCAAATCAGGAGCTGCTGGCGCGACGCTTGATGTCGTTCAGAAAAAAGGTTTTGTGCAATGCGGGATCAGTGATGGATTACCTGGGTTCTCTTATGCCGATGCTGACGGTAAGTTTTCAGGTa                                               <  2:618966/140‑1 (MQ=255)                                                                                      cGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGTCGTTCAGAAAAAAGGTTTTGTGCAATGCGGGATCAGTGATGGATTACCTGGGTTCTCTTATGCCGATGCTGACGGTAAGTTTTCAGGTAt                                              <  2:75526/140‑1 (MQ=255)                                                                                        tgctgcTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGTCGTTCAGAAAAAAGGTTTTGTGCAATGCGGGATCAGTGATGGATTACCTGGGTTCTCTTATGCCGATGCTGACGGTAAGTTTTCAGGTATTg                                            <  1:1232045/140‑1 (MQ=255)                                                                                           tgctTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGTCGTTCAGAAAAAAGGTTTTGTGCAATGCGGGATCAGTGATGGATTACCTGGGTTCTCTTATGCCGATGCTGACGGTAAGTTTTCAGGTATTGATg                                         >  1:1125303/1‑140 (MQ=255)                                                                                               tGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGTCGTTCAGAAAAAAGGTTTTGTGCAATGCGGGATCAGTGATGGATTACCTGGGTTCTCTTATGCCGATGCTGACGGTAAGTTTTCAGGTAtt                                             >  1:19732/1‑132 (MQ=255)                                                                                               tGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGTCGTTCAGAAAAAAGGTTTTGTGCAATGCGGGATCAGTGATGGATTACCTGGGTTCTCTTATGCCGATGCTGACGGTAAGTTTTCAGGTAtt                                             <  2:19732/132‑1 (MQ=255)                                                                                               tGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGTCGTTCAGAAAAAAGGTTTTGTGCAATGCGGGATCAGTGATGGATTACCTGGGTTCTCTTATGCCGATGCTGACGGTAAGTTTTCAGGTATTGATGTTGa                                     >  2:221539/1‑140 (MQ=255)                                                                                                       cAAATCAGGCGCTGCTGGCGCGACGCTTGATGTCGTTCAGAAAAAAGGTTTTGTGCAATGCGGGATCAGTGATGGATTACCTGGGTTCTCTTATGCCGATGCTGACGGTAAGTTTTCAGGTATTGATGTTGATATTTGTc                             <  1:907605/140‑1 (MQ=255)                                                                                                               gcgcTGCTGGCGCGACGCTTGATGTCGTTCAGAAAAAAGGTTTTGTGCAATGCGGGATCAGTGATGGATTACCTGGGTTCTCTTATGCCGATGCTGACGGTAAGTTTTCAGGTATTGATGTTGATATTTGTCGTGGTGtt                     >  1:1329368/1‑140 (MQ=255)                                                                                                                 gctgctGGCGCGACGCTTGATGTCGTTCAGAAAAAAGGTTTTGTGCAATGCGGGATCAGTGATGGATTACCTGGGTTCTCTTATGCCGATGCTGACGGTAAGTTTTCAGGTATTGATGTTGATATTTGTCGTGGTGTTGc                   <  1:1082797/140‑1 (MQ=255)                                                                                                                   tgctgGCGCGACGCTTGATGTCGTTCAGAAAAAAGGTTTTGTGCAATGCGGGATCAGTGATGGATTACCTGGGTTCTCTTATGCCGATGCTGACGGTAAGTTTTCAGGTATTGATGTTGATATTTGTCGTGGTGTTGCCg                 <  2:1098855/140‑1 (MQ=255)                                                                                                                     ctgGCGCGACGCTTGATGTCGTTCAGAAAAAAGGTTTTGTGCAATGCGGGATCAGTGATGGATTACCTGGGTTCTCTTATGCCGATGCTGACGGTAAGTTTTCAGGTATTGATGTTGATATTTGTCGTGGTGTTGCCgct               <  1:509037/140‑1 (MQ=255)                                                                                                                      tgGCGCGACGCTTGATGTCGTTCAGAAAAAAGGTTTTGTGCAATGCGGGATCAGTGATGGATTACCTGGGTTCTCTTATGCCGATGCTGACGGTAAGTTTTCAGGTATTGATGTTGATATTTGTCGTGGTGTTGCCgctg              <  1:264397/140‑1 (MQ=255)                                                                                                                      tgGCGCGACGCTTGATGTCGTTCAGAAAAAAGGTTTTGTGCAATGCGGGATCAGTGATGGATTACCTGGGTTCTCTTATGCCGATGCTGACGGTAAGTTTTCAGGTATTGATGTTGATATTTGTCGTGGTGTTGCCgctg              <  2:75978/140‑1 (MQ=255)                                                                                                                       ggCGCGACGCTTGATGTCGTTCAGAAAAAAGGTTTTGTGCAATGCGGGATCAGTGATGGATTACCTGGGTTCTCTTATGCCGATGCTGACGGTAAGTTTTCAGGTATTGATGTTGATATTTGTCGTGGTGTTGCCgctgc             <  2:1233848/140‑1 (MQ=255)                                                                                                                             aCGCTTGATGTCGTTCAGAAAAAAGGTTTTGTGCAATGCGGGATCAGTGATGGATTACCTGGGTTCTCTTATGCCGATGCTGACGGTAAGTTTTCAGGTATTGATGTTGATATTTGTCGTGGTGTTGCCGCTGCTGTAtt       >  1:59154/1‑140 (MQ=255)                                                                                                                             aCGCTTGATGTCGTTCAGAAAAAAGGTTTTGTGCAATGCGGGATCAGTGATGGA‑TACCTGGGTTCTCTTATGCCGATGCTGACGGTAAGTTTTCAGGTATTGATGTTGATATTTGTCGTGGTGTTGCCGCTGCTGTAttt      >  1:385530/1‑140 (MQ=255)                                                                                                                                 ttGATGTCGTTCAGAAAAAAGGTTTTGTGCAATGCGGGATCAGTGATGGATTACCTGGGTTCTCTTATGCCGATGCTGACGGTAAGTTTTCAGGTATTGATGTTGATATTTGTCGTGGTGTTGCCGCTGCTGTATTTGGt   <  1:1148002/140‑1 (MQ=255)                                                                                                                                  tGATGTCGTTCAGAAAAAAGGTTTTGTGCAATGCGGGATCAGTGATGGATTACCTGGGTTCTCTTATGCCGATGCTGACGGTAAGTTTTCAGGTATTGATGTTGATATTTGTCGTGGTGTTGCCGCTGCTGTATTTGGTg  >  1:719770/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                       TTTCCCAAACGTCCTATAACGATAATTTTCGCCTCACAGGAAGCATTATGAAAAAGATGATGATAGCCACACTGGCTGCCGCCAGCGTGCTGCTTGCCGTTGCAAATCAGGCGCTGCTGGCGCGACGCTTGATGCCGTTCAGAAAAAAGGTTTTGTGCAATGCGGGATCAGTGATGGATTACCTGGGTTCTCTTATGCCGATGCTGACGGTAAGTTTTCAGGTATTGATGTTGATATTTGTCGTGGTGTTGCCGCTGCTGTATTTGGTG  >  NC_000913/3418995‑3419263

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑