Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,597,156	T→C	Q135Q (CAA→CAG)	livK ←	L‑leucine/L‑phenylalanine ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,597,156	0	T	C	100.0%	296.0 / NA	80	Q135Q (CAA→CAG)	livK	L‑leucine/L‑phenylalanine ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (38/42);  total (38/42)

AACGCGCCAGCCCTTCGCCATACTGTTGTTTGTCGTGAATGATGGCGATGCGCTGGGGCTTCACCGTCTCAAGAATGTATTTTGCCGCCGTTGGCCCCTGGGAAGAGTCCAGCCCGGCAGTACGCATAATGTGTTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACGTATTTAATGCCGT  >  NC_000913/3597023‑3597289                                                                                                                                      |                                                                                                                                      aaCGCGCCAGCCCTTCGCCATACTGTTGTTTGTCGTGAATGATGGCGATGCGCTGGGGCTTCACCGTCTCAAGAATGTATTTTGCCGCCGTTGGCCCCTGGGAAGAGTCCAGCCCGGCAGTACGCATAATGTGCTGATaa                                                                                                                                 >  1:356256/1‑140 (MQ=255) aaCGCGCCAGCCCTTCGCCATACTGTTGTTTGTCGTGAATGATGGCGATGCGCTGGGGCTTCACCGTCTCAAGAATGTATTTTGCCGCCGTTGGCCCCTGGGAAGAGTCCAGCCCGGCAGTACGCATAATGTGCTGATaa                                                                                                                                 >  2:595231/1‑140 (MQ=255) aaCGCGCCAGCCCTTCGCCATACTGTTGTTTGTCGTGAATGATGGCGATGCGCTGGGGCTTCACCGTCTCAAGAATGTATTTTGCCGCCGTTGGCCCCTGGGAAGAGTCCAGCCCGGCAGTACGCATAATGTGCTGATaa                                                                                                                                 >  2:237585/1‑140 (MQ=255) aaCGCGCCAGCCCTTCGCCATACTGTTGTTTGTCGTGAATGATGGCGATGCGCTGGGGCTTCACCGTCTCAAGAATGTATTTTGCCGCCGTTGGCCCCTGGGAAGAGTCCAGCCCGGCAGTACGCATAATGTGCTGATaa                                                                                                                                 >  2:189213/1‑140 (MQ=255) aaCGCGCCAGCCCTTCGCCATACTGTTGTTTGTCGTGAATGATGGCGATGCGCTGGGGCTTCACCGTCTCAAGAATGTATTTTGCCGCCGTTGGCCCCTGGGAAGAGTCCAGCCCGGCAGTACGCATAATGTGCTGATaa                                                                                                                                 >  1:1363107/1‑140 (MQ=255)  aCGCGCCAGCCCTTCGCCATACTGTTGTTTGTCGTGAATGATGGCGATGCGCTGGGGCTTCACCGTCTCAAGAATGTATTTTGCCGCCGTTGGCCCCTGGGAAGAGTCCAGCCCGGCAGTACGCATAATGTGCTGATAAc                                                                                                                                <  2:1363107/140‑1 (MQ=255)      gcCAGCCCTTCGCCATACTGTTGTTTGTCGTGAATGATGGCGATGCGCTGGGGCTTCACCGTCTCAAGAATGTATTTTGCCGCCGTTGGCCCCTGGGAAGAGTCCAGCCCGGCAGTACGCATAATGTGCTGATAACcgcg                                                                                                                            >  1:1318610/1‑140 (MQ=255)      gcCAGCCCTTCGCCATACTGTTGTTTGTCGTGAATGATGGCGATGCGCTGGGGCTTCACCGTCTCAAGAATGTATTTTGCCGCCGTTGGCCCCTGGGAAGAGTCCAGCCCGGCAGTACGCATAATGTGCTGATAACcgcg                                                                                                                            >  1:661982/1‑140 (MQ=255)            ccTTCGCCATACTGTTGTTTGTCGTGAATGATGGCGATGCGCTGGGGCTTCACCGTCTCAAGAATGTATTTTGCCGCCGTTGGCCCCTGGGAAGAGTCCAGCCCGGCAGTACGCATAATGTGCTGATAACCGCGTTGGGt                                                                                                                      <  2:1223250/140‑1 (MQ=255)            ccTTCGCCATACTGTTGTTTGTCGTGAATGATGGCGATGCGCTGGGGCTTCACCGTCTCAAGAATGTATTTTGCCGCCGTTGGCCCCTGGGAAGAGTCCAGCCCGGCAGTACGCATAATGTGCTGATAACCGCGTTGGGt                                                                                                                      <  2:851784/140‑1 (MQ=255)            ccTTCGCCATACTGTTGTTTGTCGTGAATGATGGCGATGCGCTGGGGCTTCACCGTCTCAAGAATGTATTTTGCCGCCGTTGGCCCCTGGGAAGAGTCCAGCCCGGCAGTACGCATAATGTGCTGATAACCGCGTTGGGt                                                                                                                      <  2:1189163/140‑1 (MQ=255)                cGCCATACTGTTGTTTGTCGTGAATGATGGCGATGCGCTGGGGCTTCACCGTCTCAAGAATGTATTTTGCCGCCGTTGGCCCCTGGGAAGAGTCCAGCCCGGCAGTACGCATAATGTGCTGATAACCGCGTTGGGTCAGc                                                                                                                  >  2:1195780/1‑140 (MQ=255)                 gCCATACTGTTGTTTGTCGTGAATGATGGCGATGCGCTGGGGCTTCACCGTCTCAAGAATGTATTTTGCCGCCGTTGGCCCCTGGGAAGAGTCCAGCCCGGCAGTACGCATAATGTGCTGATAACCGCGTTGGGTCAGCt                                                                                                                 <  1:585801/140‑1 (MQ=255)                 gCCATACTGTTGTTTGTCGTGAATGATGGCGATGCGCTGGGGCTTCACCGTCTCAAGAATGTATTTTGCCGCCGTTGGCCCCTGGGAAGAGTCCAGCCCGGCAGTACGCATAATGTGCTGATAACCGCGTTGGGTCAGCt                                                                                                                 <  1:189213/140‑1 (MQ=255)                     tACTGTTGTTTGTCGTGAATGATGGCGATGCGCTGGGGCTTCACCGTCTCAAGAATGTATTTTGCCGCCGTTGGCCCCTGGGAAGAGTCCAGCCCGGCAGTACGCATAATGTGCTGATAACCGCGTTGGGTCAGCTCCgg                                                                                                             >  2:1008646/1‑140 (MQ=255)                      aCTGTTGTTTGTCGTGAATGATGGCGATGCGCTGGGGCTTCACCGTCTCAAGAATGTATTTTGCCGCCGTTGGCCCCTGGGAAGAGTCCAGCCCGGCAGTACGCATAATGTGCTGATAACCGCGTTGGGTCAGCTCCggg                                                                                                            >  2:614234/1‑140 (MQ=255)                          ttgtttgtCGTGAATGATGGCGATGCGCTGGGGCTTCGCCGTCTCAAGAATGTATTTTGCCGCCGTTGGCCCCTGGGAAGAGTCCAGCCCGGCAGTACGCATAATGTGCTGATAACCGCGTTGGGTCAGCTCCGGGTTgg                                                                                                        >  1:316626/1‑140 (MQ=255)                          ttgtttgtCGTGAATGATGGCGATGCGCTGGGGCTTCACCGTCTCAAGAATGTATTTTGCCGCCGTTGGCCCCTGGGAAGAGTCCAGCCCGGCAGTACGCATAATGTGCTGATAACCGCGTTGGGTCAGCTCCGGGTTgg                                                                                                        >  2:936803/1‑140 (MQ=255)                          ttgtttgtCGTGAATGATGGCGATGCGCTGGGGCTTCACCGTCTCAAGAATGTATTTTGCCGCCGTTGGCCCCTGGGAAGAGTCCAGCCCGGCAGTACGCATAATGTGCTGATAACCGCGTTGGGTCAGCTCCGGGTTgg                                                                                                        >  2:919050/1‑140 (MQ=255)                               tgtCGTGAATGATGGCGATGCGCTGGGGCTTCACCGTCTCAAGAATGTATTTTGCCGCCGTTGGCCCCTGGGAAGAGTCCAGCCCGGCAGTACGCATAATGTGCTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCt                                                                                                   >  1:753433/1‑140 (MQ=255)                               tgtCGTGAATGATGGCGATGCGCTGGGGCTTCACCGTCTCAAGAATGTATTTTGCCGCCGTTGGCCCCTGGGAAGAGTCCAGCCCGGCAGTACGCATAATGTGCTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCt                                                                                                   <  2:796360/140‑1 (MQ=255)                                  cGTGAATGATGGCGATGCGCTGGGGCTTCACCGTCTCAAGAATGTATTTTGCCGCCGTTGGCCCCTGGGAAGAGTCCAGCCCGGCAGTACGCATAATGTGCTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTccc                                                                                                >  2:691042/1‑140 (MQ=255)                                  cGTGAATGATGGCGATGCGCTGGGGCTTCACCGTCTCAAGAATGTATTTTGCCGCCGTTGGCCCCTGGGAAGAGTCCAGCCCGGCAGTACGCATAATGTGCTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTcc                                                                                                 >  2:1205007/1‑139 (MQ=255)                                  cGTGAATGATGGCGATGCGCTGGGGCTTCACCGTCTCAAGAATGTATTTTGCCGCCGTTGGCCCCTGGGAAGAGTCCAGCCCGGCAGTACGCATAATGTGCTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTcc                                                                                                 <  1:1205007/139‑1 (MQ=255)                                        tgatgGCGATGCGCTGGGGCTTCACCGTCTCAAGAATGTATTTTGCCGCCGTTGGCCCCTGGGAAGAGTCCAGCCCGGCAGTACGCATAATGTGCTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCgag                                                                                          <  2:1206862/140‑1 (MQ=255)                                        tgatgGCGATGCGCTGGGGCTTCACCGTCTCAAGAATGTATTTTGCCGCCGTTGGCCCCTGGGAAGAGTCCAGCCCGGCAGTACGCATAATGTGCTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCgag                                                                                          <  2:1176031/140‑1 (MQ=255)                                        tgatgGCGATGCGCTGGGGCTTCACCGTCTCAAGAATGTATTTTGCCGCCGTTGGCCCCTGGGAAGAGTCCAGCCCGGCAGTACGCATAATGTGCTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCgag                                                                                          <  2:1318610/140‑1 (MQ=255)                                          atgGCGATGCGCTGGGGCTTCACCGTCTCAAGAATGTATTTTGCCGCCGTTGGCCCCTGGGAAGAGTCCAGCCCGGCAGTACGCATAATGTGCTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGat                                                                                        <  1:719823/140‑1 (MQ=255)                                           tgGCGATGCGCTGGGGCTTCACCGTCTCAAGAATGTATTTTGCCGCCGTTGGCCCCTGGGAAGAGTCCAGCCCGGCAGTACGCATAATGTGCTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGatc                                                                                       <  2:128788/140‑1 (MQ=255)                                            ggCGATGCGCTGGGGCTTCACCGTCTCAAGAATGTATTTTGCCGCCGTTGGCCCCTGGGAAGAGTCCAGCCCGGCAGTACGCATAATGTGCTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGatca                                                                                      >  2:683722/1‑140 (MQ=255)                                             gCGATGCGCTGGGGCTTCACCGTCTCAAGAATGTATTTTGCCGCCGTTGGCCCCTGGGAAGAGTCCAGCCCGGCAGTACGCATAATGTGCTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGatcat                                                                                     <  2:1033452/140‑1 (MQ=255)                                                aTGCGCTGGGGCTTCACCGTCTCAAGAATGTATTTTGCCGCCGTTGGCCCCTGGGAAGAGTCCAGCCCGGCAGTACGCATAATGTGCTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAg                                                                                  >  1:770353/1‑140 (MQ=255)                                                aTGCGCTGGGGCTTCACCGTCTCAAGAATGTATTTTGCCGCCGTTGGCCCCTGGGAAGAGTCCAGCCCGGCAGTACGCATAATGTGCTGAT‑ACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGa                                                                                 <  1:315042/140‑1 (MQ=255)                                                   cgcTGGGGCTTCACCGTCTCAAGAATGTATTTTGCCGCCGTTGGCCCCTGGGAAGAGTCCAGCCCGGCAGTACGCATAATGTGCTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAAt                                                                               >  2:414414/1‑140 (MQ=255)                                                   cgcTGGGGCTTCACCGTCTCAAGAATGTATTTTGCCGCCGTTGGCCCCTGGGAAGAGTCCAGCCCGGCAGTACGCATAATGTGCTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAAt                                                                               >  1:934287/1‑140 (MQ=255)                                                        gggCTTCACCGTCTCAAGAATGTATTTTGCCGCCGTTGGCCCCTGGGAAGAGTCCAGCCCGGCAGTACGCATAATGTGCTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCtt                                                                          <  1:683722/140‑1 (MQ=255)                                                        gggCTTCACCGTCTCAAGAATGTATTTTGCCGCCGTTGGCCCCTGGGAAGAGTCCAGCCCGGCAGTACGCATAATGTGCTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCtt                                                                          <  1:237585/140‑1 (MQ=255)                                                        gggCTTCACCGTCTCAAGAATGTATTTTGCCGCCGTTGGCCCCTGGGAAGAGTCCAGCCCGGCAGTACGCATAATGTGCTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCtt                                                                          >  1:294158/1‑140 (MQ=255)                                                         ggCTTCACCGTCTCAAGAATGTATTTTGCCGCCGTTGGCCCCTGGGAAGAGTCCAGCCCGGCAGTACGCATAATGTGCTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTc                                                                         <  2:770353/140‑1 (MQ=255)                                                          gCTTCACCGTCTCAAGAATGTATTTTGCCGCCGTTGGCCCCTGGGAAGAGTCCAGCCCGGCAGTACGCATAATGTGCTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCg                                                                        <  2:934287/140‑1 (MQ=255)                                                          gCTTCACCGTCTCAAGAATGTATTTTGCCGCCGTTGGCCCCTGGGAAGAGTCCAGCCCGGCAGTACGCATAATGTGCTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCg                                                                        <  2:356256/140‑1 (MQ=255)                                                          gCTTCACCGTCTCAAGAATGTATTTTGCCGCCGTTGGCCCCTGGGAAGAGTCCAGCCCGGCAGTACGCATAATGTGCTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCg                                                                        >  1:78166/1‑140 (MQ=255)                                                            ttCACCGTCTCAAGAATGTATTTTGCCGCCGTTGGCCCCTGGGAAGAGTCCAGCCCGGCAGTACGCATAATGTGCTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTc                                                                      >  1:603979/1‑140 (MQ=255)                                                               aCCGTCTCAAGAATGTATTTTGCCGCCGTTGGCCCCTGGGAAGAGTCCAGCCCGGCAGTACGCATAATGTGCTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTc                                                                   >  2:520934/1‑140 (MQ=255)                                                                ccGTCTCAAGAATGTATTTTGCCGCCGTTGGCCCCTGGGAAGAGTCCAGCCCGGCAGTACGCATAATGTGCTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCa                                                                  <  1:1195780/140‑1 (MQ=255)                                                                  gTCTCAAGAATGTATTTTGCCGCCGTTGGCCCCTGGGAAGAGTCCAGCCCGGCAGTACGCATAATGTGCTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATa                                                                >  2:1158919/1‑140 (MQ=255)                                                                  gTCTCAAGAATGTATTTTGCCGCCGTTGGCCCCTGGGAAGAGTCCAGCCCGGCAGTACGCATAATGTGCTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATa                                                                >  2:728714/1‑140 (MQ=255)                                                                  gTCTCAAGAATGTATTTTGCCGCCGTTGGCCCCTGGGAAGAGTCCAGCCCGGCAGTACGCATAATGTGCTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATa                                                                >  1:371950/1‑140 (MQ=255)                                                                    ctcAAGAATGTATTTTGCCGCCGTTGGCCCCTGGGAAGAGTCCAGCCCGGCAGTACGCATAATGTGCTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGa                                                              >  2:1094396/1‑140 (MQ=255)                                                                           aTGTATTTTGCCGCCGTTGGCCCCTGGGAAGAGTCCAGCCCGGCAGTACGCATAATGTGCTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGa                                                       >  1:758501/1‑140 (MQ=255)                                                                            tGTATTTTGCCGCCGTTGGCCCCTGGGAAGAGTCCAGCCCGGCAGTACGCATAATGTGCTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGAc                                                      <  2:316626/140‑1 (MQ=255)                                                                            tGTATTTTGCCGCCGTTGGCCCCTGGGAAGAGTCCAGCCCGGCAGTACGCATAATGTGCTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGAc                                                      <  1:671078/140‑1 (MQ=255)                                                                                 tttGCCGCCGTTGGCCCCTGGGAAGAGTCCAGCCCGGCAGTACGCATAATGTGCTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAgg                                                 <  2:603979/140‑1 (MQ=255)                                                                                 tttGCCGCCGTTGGCCCCTGGGAAGAGTCCAGCCCGGCAGTACGCATAATGTGCTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAgg                                                 <  1:414414/140‑1 (MQ=255)                                                                                 tttGCCGCCGTTGGCCCCTGGGAAGAGTCCAGCCCGGCAGTACGCATAATGTGCTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTACCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAgg                                                 <  1:1008646/140‑1 (MQ=255)                                                                                    gccgccGTTGGCCCCTGGGAAGAGTCCAGCCCGGCAGTACGCATAATGTGCTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAG‑‑‑‑‑‑TTCGTCTTCATAGATATCTGACGCa                                                   <  2:1200278/129‑1 (MQ=255)                                                                                    gccgccGTTGGCCCCTGGGAAGAGTCCAGCCCGGCAGTACGCATAATGTGCTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAG‑‑‑‑‑‑TTCGTCTTCATAGATATCTGACGCa                                                   >  1:1200278/1‑129 (MQ=255)                                                                                       gccgTTGGCCCCTGGGAAGAGTCCAGCCCGGCAGTACGCATAATGTGCTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGt                                           <  1:520934/140‑1 (MQ=255)                                                                                       gccgTTGGCCCCTGGGAAGAGTCCAGCCCGGCAGTACGCATAATGTGCTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGt                                           <  2:1342517/140‑1 (MQ=255)                                                                                         cgTTGGCCCCTGGGAAGAGTCCAGCCCGGCAGTACGCATAATGTGCTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTag                                         <  2:758501/140‑1 (MQ=255)                                                                                         cgTTGGCCCCTGGGAAGAGTCCAGCCCGGCAGTACGCATAATGTGCTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTag                                         <  2:371950/140‑1 (MQ=255)                                                                                         cgTTGGCCCCTGGGAAGAGTCCAGCCCGGCAGTACGCATAATGTGCTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTag                                         <  1:919050/140‑1 (MQ=255)                                                                                            tGGCCCCTGGGAAGAGTCCAGCCCGGCAGTACGCATAATGTGCTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTagaag                                      <  2:307611/140‑1 (MQ=255)                                                                                            tGGCCCCTGGGAAGAGTCCAGCCCGGCAGTACGCATAATGTGCTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTagaag                                      <  2:78166/140‑1 (MQ=255)                                                                                            tGGCCCCTGGGAAGAGTCCAGCCCGGCAGTACGCATAATGTGCTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTagaag                                      <  1:595231/140‑1 (MQ=255)                                                                                                 ccTGGGAAGAGTCCAGCCCGGCAGTACGCATAATGTGCTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAgaagaagaa                                 <  1:1065842/140‑1 (MQ=255)                                                                                                 ccTGGGAAGAGTCCAGCCCGGCAGTACGCATAATGTGCTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAgaagaagaa                                 <  1:853492/140‑1 (MQ=255)                                                                                                   tGGGAAGAGTCCAGCCCGGCAGTACGCATAATGTGCTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAaca                               >  1:74343/1‑140 (MQ=255)                                                                                                     ggAAGAGTCCAGCCCGGCAGTACGCATAATGTACTGATAACCGCGCTGGGTCAGCTCCGGGTTGGTCGCTCGCGGCGAGATCATCAGAATACCTGCGTCTTCACAGATATCTGGCGGAGGCTGGGTAGAAGAAGAAcaca                             <  2:1336405/140‑1 (MQ=255)                                                                                                          agTCCAGCCCGGCAGTACGCATAATGTGCTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTagaag                                      <  2:1059455/126‑1 (MQ=255)                                                                                                          agTCCAGCCCGGCAGTACGCATAATGTGCTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTagaag                                      >  1:1059455/1‑126 (MQ=255)                                                                                                          agTCCAGCCCGGCAGTACGCATAATGTGCTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGa                        >  2:142091/1‑140 (MQ=255)                                                                                                          agTCCAGCCCGGCAGTACGCATAATGTGCTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGa                        >  1:974254/1‑140 (MQ=255)                                                                                                              cAGCCCGGCAGTACGCATAATGTGCTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTAGCTCCCGGCGAGGTCATCAGAATTCCTTCGTCTTCATAGATATCTGACGTAGGCTGGGTAGAAGAAGAACGCAGATGACCaa                    <  2:753433/140‑1 (MQ=255)                                                                                                               aGCCCGGCAGTACGCATAATGTGCTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAAt                   <  1:936803/140‑1 (MQ=255)                                                                                                                gCCCGGCAGTACGCATAATGTGCTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATa                  <  2:294158/140‑1 (MQ=255)                                                                                                                gCCCGGCAGTACGCATAATGTGCTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATa                  <  2:1165532/140‑1 (MQ=255)                                                                                                                gCCCGGCAGTACGCATAATGTGCTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATa                  >  1:1056495/1‑140 (MQ=255)                                                                                                                      cAGTACGCATAATGTGCTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACGTAt            >  1:15129/1‑140 (MQ=255)                                                                                                                            gCATAATGTGCTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACGTATTTAATg      <  1:691042/140‑1 (MQ=255)                                                                                                                                aaTGTGCTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACGTATTTAATGCCGt  >  1:944764/1‑140 (MQ=255)                                                                                                                                aaTGTGCTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACGTATTTAATGCCGt  >  1:173930/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                      AACGCGCCAGCCCTTCGCCATACTGTTGTTTGTCGTGAATGATGGCGATGCGCTGGGGCTTCACCGTCTCAAGAATGTATTTTGCCGCCGTTGGCCCCTGGGAAGAGTCCAGCCCGGCAGTACGCATAATGTGTTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACGTATTTAATGCCGT  >  NC_000913/3597023‑3597289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑