Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,552,368	2 bp→TC	intergenic (+377/+29)	fdnI → / ← yddM	formate dehydrogenase N subunit gamma/putative DNA‑binding transcriptional regulator YddM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,552,368	0	C	T	100.0%	156.7 / NA	46	intergenic (+377/+30)	fdnI/yddM	formate dehydrogenase N subunit gamma/putative DNA‑binding transcriptional regulator YddM
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/22);  total (24/22)
*	NC_000913	1,552,369	0	T	C	100.0%	160.8 / NA	46	intergenic (+378/+29)	fdnI/yddM	formate dehydrogenase N subunit gamma/putative DNA‑binding transcriptional regulator YddM
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/22);  total (24/22)

GCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTTCTGCCTCTGCCAGACTCCAGGCGTTTTGCAGATTCAGCCACATTTGCGGCGAACT  >  NC_000913/1552249‑1552499                                                                                                                        ||                                                                                                                                   gCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTTTATAAAGt                                                                                                                 <  1:1031604/140‑1 (MQ=2) gCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTTTATAAAGt                                                                                                                 >  2:56549/1‑140 (MQ=17)   cGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTTTATAAAGTGc                                                                                                               <  2:1229646/140‑1 (MQ=12)      aTGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTTTATAAAGTGCCAt                                                                                                            >  1:1036565/1‑140 (MQ=255)      aTGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTTTATAAAGTGCCAt                                                                                                            >  1:762143/1‑140 (MQ=255)              tGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTTTATAAAGTGCCATTTTTTTAt                                                                                                    >  1:1311810/1‑140 (MQ=255)                      ttATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAAc                                                                                            >  1:1338193/1‑140 (MQ=255)                          ccGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGa                                                                                        <  1:648835/140‑1 (MQ=255)                             gCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACgg                                                                                     >  2:215716/1‑140 (MQ=255)                              cATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGc                                                                                    >  2:1048420/1‑140 (MQ=255)                                        cTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGa                                                                          <  1:215716/140‑1 (MQ=255)                                           cAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGa                                                                                        <  1:313951/123‑1 (MQ=255)                                           cAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGa                                                                                        >  2:313951/1‑123 (MQ=255)                                           cAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGacac                                                                       <  1:434881/140‑1 (MQ=255)                                           cAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGacac                                                                       <  2:762143/140‑1 (MQ=255)                                           cAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGacac                                                                       <  2:1036565/140‑1 (MQ=255)                                                    tAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAATTTCAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGt                                                              >  1:1221323/1‑140 (MQ=255)                                                       aTTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTTTATAAAGTACCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATcc                                                                   <  2:306246/132‑1 (MQ=255)                                                       aTTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTTTATAAAGTACCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATcc                                                                   >  1:306246/1‑132 (MQ=255)                                                        ttGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGATTCAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGttttt                                                          >  1:1391138/1‑140 (MQ=255)                                                           cAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTtct                                                       >  2:1230226/1‑140 (MQ=255)                                                            aGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTtctg                                                      >  2:185988/1‑140 (MQ=255)                                                             gTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTTTATAAATTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTtctgc                                                     <  2:1249499/140‑1 (MQ=255)                                                             gTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTtctgc                                                     <  2:5006/140‑1 (MQ=255)                                                             gTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTtctgc                                                     <  1:926564/140‑1 (MQ=255)                                                               gAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTtctgcct                                                   <  1:185988/140‑1 (MQ=255)                                                                        ggCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTTCTGCCTCTGCCAGAc                                          >  1:238580/1‑140 (MQ=255)                                                                          ccTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTTCTGCCTCTGCCAGACTc                                        >  2:925106/1‑140 (MQ=255)                                                                              aTAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTTCTGCCTCTGCCa                                             >  2:1392313/1‑131 (MQ=255)                                                                              aTAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTTCTGCCTCTGCCa                                             <  1:1392313/131‑1 (MQ=255)                                                                              aTAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTTCTGCCTCTGCCAGACTCCAgg                                    >  2:1028926/1‑140 (MQ=255)                                                                              aTAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTTCTGCCTCTGCCAGACTCCAgg                                    >  1:181606/1‑140 (MQ=255)                                                                                   cGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTTCTGCCTCTGCCAGACTCCAGGCGttt                               >  1:330945/1‑140 (MQ=255)                                                                                    gTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTTCTGCCTCTGCCAGACTCCAGGCGtttt                              <  2:1221323/140‑1 (MQ=255)                                                                                        cgcATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTTCTGCCTCTGCCAGACTCCAGGCGTTTTGCAg                          >  2:896336/1‑140 (MQ=255)                                                                                        cgcATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTTCTGCCTCTGCCAGACTCCAGGCGTTTTGCAg                          >  1:290513/1‑140 (MQ=255)                                                                                        cgcATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTTCTGCCTCTGCCAGACTCCAGGCGTTTTGCAg                          >  1:1288395/1‑140 (MQ=255)                                                                                         gcATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTTCTGCCTCTGCCAGACTCCAGGCGTTTTGCAGa                         <  2:1311810/140‑1 (MQ=255)                                                                                               ggCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTTCTGCCTCTGCCAGACTCCAGGCGTTTTGCAGATTCAGc                   <  1:1028926/140‑1 (MQ=255)                                                                                                       tGCGTTTGTCATCAGTTTCAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTTCTGCCTCTGCCAGACTCCAGGCGTTTTGCAGATTCAGCCACATTTg           <  1:925106/140‑1 (MQ=255)                                                                                                         cGTTTGTCATCAGTTTCAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTTCTGCCTCTGCCAGACTCCAGGCGTTTTGCAGATTCAGCCACATTTgcg         <  2:238580/140‑1 (MQ=255)                                                                                                            ttGTCATCAGTTTCAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTTCTGCCTCTGCCAGACTCCAGGCGTTTTGCAGATTCAGCCACATTTgcggcg      <  2:290513/140‑1 (MQ=255)                                                                                                            ttGTCATCAGTTTCAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTTCTGCCTCTGCCAGACTCCAGGCGTTTTGCAGATTCAGCCACATTTgcggcg      <  1:1170221/140‑1 (MQ=255)                                                                                                            ttGTCATCAGTTTCAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTTCTGCCTCTGCCAGACTCCAGGCGTTTTGCAGATTCAGCCACATTTgcggcg      <  1:1136519/140‑1 (MQ=255)                                                                                                              gTCATCAGTTTCAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTTCTGCCTCTGCCAGACTCCAGGCGTTTTGCAGATTCAGCCACATTTGCGGCGaa    >  2:1210242/1‑140 (MQ=255)                                                                                                                catcaGTTTCAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTTCTGCCTCTGCCAGACTCCAGGCGTTTTGCAGATTCAGCCACATTTGCGGCGAACt  >  1:238313/1‑140 (MQ=255)                                                                                                                        ||                                                                                                                                   GCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTTCTGCCTCTGCCAGACTCCAGGCGTTTTGCAGATTCAGCCACATTTGCGGCGAACT  >  NC_000913/1552249‑1552499

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑