Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,474,315	(C)6→5	coding (95/861 nt)	pdxI →	pyridoxine 4‑dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,474,310	0	C	.	100.0%	352.3 / NA	81	coding (90/861 nt)	pdxI	pyridoxine 4‑dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (38/43);  total (38/43)

TCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCAGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGGCCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCACGTCACCAATCAGATTATCCGCGAAGCGCTTTATCCTTAC  >  NC_000913/1474175‑1474445                                                                                                                                        |                                                                                                                                        tCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCAGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGGccccc                                                                                                                                     <  1:508260/140‑1 (MQ=255)          aTTTTTCTTAATCCGTTTTATTACAGGGCAGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGG‑CCCCCACGAGATCg                                                                                                                           >  1:41288/1‑140 (MQ=255)          aTTTTTCTTAATCCGTTTTATTACAGGGCAGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGG‑CCCCCACGAGATCg                                                                                                                           >  2:750836/1‑140 (MQ=255)          aTTTTTCTTAATCCGTTTTATTACAGGGCAGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGG‑CCCCCACGAGATCg                                                                                                                           >  2:600524/1‑140 (MQ=255)          aTTTTTCTTAATCCGTTTTATTACAGGGCAGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGG‑CCCCCACGAGATCg                                                                                                                           >  1:288647/1‑140 (MQ=255)            ttttCTTAATCCGTTTTATTACAGGGCAGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGG‑CCCCCACGAGATCGcc                                                                                                                         >  1:148163/1‑140 (MQ=255)              ttCTTAATCCGTTTTATTACAGGGCAGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGG‑CCCCCACGAGATCGCCAc                                                                                                                       <  2:924442/140‑1 (MQ=255)              ttCTTAATCCGTTTTATTACAGGGCAGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGG‑CCCCCACGAGATCGCCAc                                                                                                                       <  2:718298/140‑1 (MQ=255)              ttCTTAATCCGTTTTATTACAGGGCAGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGG‑CCCCCACGAGATCGCCAc                                                                                                                       <  2:135432/140‑1 (MQ=255)               tCTTAATCCGTTTTATTACAGGGCAGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGG‑CCCCCACGAGAt                                                                                                                             >  2:426366/1‑133 (MQ=255)               tCTTAATCCGTTTTATTACAGGGCAGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGG‑CCCCCACGAGAt                                                                                                                             <  1:426366/133‑1 (MQ=255)                cTTAATCCGTTTTATTACAGGGCAGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGG‑CCCCCACGAGATCGCCACGt                                                                                                                     <  1:750836/140‑1 (MQ=255)                     tCCGTTTTATTACAGGGCAGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGG‑CCCCCACGAGATCGCCACGTCGCta                                                                                                                <  2:742587/140‑1 (MQ=255)                         ttttATTACAGGGCAGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGG‑CCCCCACGAGATCGCCACGTCGCTATaa                                                                                                             >  1:16721/1‑139 (MQ=255)                         ttttATTACAGGGCAGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGG‑CCCCCACGAGATCGCCACGTCGCTATaa                                                                                                             <  2:16721/139‑1 (MQ=255)                                   gggcagggTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGG‑CCCCCACGAGATCGCCACGTCGc                                                                                                                  <  1:993639/124‑1 (MQ=255)                                   gggcagggTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGG‑CCCCCACGAGATCGCCACGTCGc                                                                                                                  >  2:993639/1‑124 (MQ=255)                                   gggcagggTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGG‑CCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGt                                                                                                  >  1:1008465/1‑140 (MQ=255)                                   gggcagggTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGG‑CCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGt                                                                                                  >  1:1064685/1‑140 (MQ=255)                                      cagggTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGG‑CCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAg                                                                                               >  1:937572/1‑140 (MQ=255)                                       agggTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGG‑CCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAgg                                                                                              <  1:77996/140‑1 (MQ=255)                                         ggcgCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGG‑CCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGgcg                                                                                            >  2:594479/4‑140 (MQ=255)                                         ggTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGG‑CCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGgcg                                                                                            <  2:1122997/140‑1 (MQ=255)                                         ggTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGG‑CCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGgcg                                                                                            <  1:1046446/140‑1 (MQ=255)                                         ggTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGG‑CCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGgcg                                                                                            <  1:535922/140‑1 (MQ=255)                                           tGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGG‑CCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCt                                                                                          >  2:1006490/1‑140 (MQ=255)                                           tGCGATGAGCACCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGG‑CCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCt                                                                                          >  2:784574/1‑140 (MQ=255)                                              gATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGG‑CCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGc                                                                                       >  1:911864/1‑140 (MQ=255)                                               aTGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGG‑CCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCa                                                                                      >  2:631363/1‑140 (MQ=255)                                                       caATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGG‑CCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGt                                                                              >  1:17231/1‑140 (MQ=255)                                                       caATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGG‑CCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGt                                                                              >  2:1067057/1‑140 (MQ=255)                                                         aTACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGG‑CCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCa                                                                            <  1:1067057/140‑1 (MQ=255)                                                         aTACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGG‑CCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCa                                                                            <  2:17231/140‑1 (MQ=255)                                                           aCATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCACCTGGCAGGTCCTGGAGTTTTTGG‑CCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAAt                                                                          <  2:1008465/140‑1 (MQ=255)                                                            cATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGG‑CCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATc                                                                         >  1:951740/1‑140 (MQ=255)                                                            cATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGG‑CCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATc                                                                         <  2:148163/140‑1 (MQ=255)                                                            cATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGG‑CCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATc                                                                         <  2:274577/140‑1 (MQ=255)                                                                 aCTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGG‑CCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGt                                                                              <  2:305061/130‑1 (MQ=255)                                                                 aCTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGG‑CCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGt                                                                              >  1:305061/1‑130 (MQ=255)                                                                 aCTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGG‑CCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAAt                                                                          <  1:909224/134‑1 (MQ=255)                                                                 aCTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGG‑CCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAAt                                                                          >  2:909224/1‑134 (MQ=255)                                                                 aCTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGG‑CCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATAtt                                                                    >  1:946843/1‑140 (MQ=255)                                                                 aCTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGG‑CCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATAtt                                                                    >  1:858424/1‑140 (MQ=255)                                                                    ctcGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGG‑CCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGt                                                                              >  2:87656/1‑127 (MQ=255)                                                                    ctcGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGG‑CCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGt                                                                              <  1:87656/127‑1 (MQ=255)                                                                      cGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGG‑CCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATAc                                                               >  2:158942/1‑140 (MQ=255)                                                                       ggTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGG‑CCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATAcc                                                              >  1:203899/1‑140 (MQ=255)                                                                       ggTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGG‑CCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATAcc                                                              >  1:910153/1‑140 (MQ=255)                                                                       ggTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGG‑CCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATAcc                                                              >  2:219081/1‑140 (MQ=255)                                                                        gTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGG‑CCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCa                                                             >  1:615857/1‑140 (MQ=255)                                                                          aCAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGG‑CCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGc                                                           <  1:321673/140‑1 (MQ=255)                                                                          aCAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGG‑CCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGc                                                           <  2:615857/140‑1 (MQ=255)                                                                          aCAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGG‑CCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGc                                                           <  1:784574/140‑1 (MQ=255)                                                                          aCAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGG‑CCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGc                                                           <  1:631363/140‑1 (MQ=255)                                                                                  cGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGG‑CCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTa                                                   <  2:1074674/140‑1 (MQ=255)                                                                                   gTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGG‑CCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTAt                                                  >  2:72091/1‑140 (MQ=255)                                                                                   gTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGG‑CCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTAt                                                  >  2:95112/1‑140 (MQ=255)                                                                                       aCCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGG‑CCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTc                                              >  2:514863/1‑140 (MQ=255)                                                                                           tCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGG‑CCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCa                                          <  2:911864/140‑1 (MQ=255)                                                                                           tCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGG‑CCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCa                                          <  1:1006490/140‑1 (MQ=255)                                                                                           tCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGG‑CCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCa                                          <  1:201800/140‑1 (MQ=255)                                                                                              tGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGG‑CCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCACGt                                       <  2:910153/140‑1 (MQ=255)                                                                                                gTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGG‑CCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCACGTCa                                     <  2:41288/140‑1 (MQ=255)                                                                                                   aTGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGG‑CCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCACGTCACCa                                  <  1:158942/140‑1 (MQ=255)                                                                                                   aTGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGG‑CCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCACGTCACCa                                  <  1:594479/140‑1 (MQ=255)                                                                                                   aTGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGG‑CCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCACGTCACCa                                  >  2:176896/1‑140 (MQ=255)                                                                                                   aTGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGG‑CCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCACGTCACCa                                  <  2:1064685/140‑1 (MQ=255)                                                                                                     ggCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGG‑CCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCACGTCACCAAt                                <  2:372670/140‑1 (MQ=255)                                                                                                       cgcgATGCAACTGGCAGGTCCTGGAGTTTTTGG‑CCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGTGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCACGTCACCAATCa                              >  2:573353/1‑140 (MQ=255)                                                                                                        gcgATGCAACTGGCAGGTCCTGGAGTTTTTGG‑CCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATGCCAGCGACTTTTATGGTCCGCACGTCACCAATCAg                             <  2:937572/140‑1 (MQ=255)                                                                                                         cgATGCAACTGGCAGGTCCTGGAGTTTTTGG‑CCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCACGTCACCAATCAGa                            <  1:600524/140‑1 (MQ=255)                                                                                                          gATGCAACTGGCAGGTCCTGGAGTTTTTGG‑CCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCACGTCACCAATCAGAt                           >  1:654331/1‑140 (MQ=255)                                                                                                                  tGGCAGGTCCTGGAGTTTTTGG‑CCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCACGTCACCAATCAGATTATCcgcg                   <  2:951740/140‑1 (MQ=255)                                                                                                                    gCAGGTCCTGGAGTTTTTGG‑CCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCACGTCACCAATCAGATTATCCGCGaa                 <  2:492090/140‑1 (MQ=255)                                                                                                                      aGGTCCTGGAGTTTTTGG‑CCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCACGTCACCAATCAGATTATCCGCGAAgc               >  2:322788/1‑140 (MQ=255)                                                                                                                        gTCCTGGAGTTTTTGG‑CCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCACGTCACCAATCAGATTATCCGCGAAgcgc             >  1:900554/1‑140 (MQ=255)                                                                                                                         tCCTGGAGTTTTTGG‑CCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCACGTCACCAATCAGATTATCCGCGAAGCGCt            >  2:1033731/1‑140 (MQ=255)                                                                                                                         tCCTGGAGTTTTTGG‑CCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCACGTCACCAATCAGATTATCCGCGAAGCGCt            <  1:322788/140‑1 (MQ=255)                                                                                                                          ccTGGAGTTTTTGG‑CCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCACGTCACCAATCAGATTATCCGCGAAGCGCtt           <  2:858424/140‑1 (MQ=255)                                                                                                                          ccTGGAGTTTTTGG‑CCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCACGTCACCAATCAGATTATCCGCGAAGCGCtt           <  2:900554/140‑1 (MQ=255)                                                                                                                          ccTGGAGTTTTTGG‑CCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCACGTCACCAATCAGATTATCCGCGAAGCGCtt           <  1:1033731/140‑1 (MQ=255)                                                                                                                               aGTTTTTGG‑CCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCACGTCACCAATCAGATTATCCGCGAAGCGCTTTATcc      <  2:288647/140‑1 (MQ=255)                                                                                                                                  ttttGG‑CCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCACGTCACCAATCAGATTATCCGCGAAGCGCTTTATCCTTa   <  2:203899/140‑1 (MQ=255)                                                                                                                                  ttttGG‑CCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCACGTCACCAATCAGATTATCCGCGAAGCGCTTTATCCTTa   >  2:7014/1‑140 (MQ=255)                                                                                                                                   tttGG‑CCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCACGTCACCAATCAGATTATCCGCGAAGCGCTTTATCCTTAc  >  1:1117888/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                        TCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCAGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGGCCCCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCACGTCACCAATCAGATTATCCGCGAAGCGCTTTATCCTTAC  >  NC_000913/1474175‑1474445

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑