| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,770,856 | (T)6→5 | coding (242/357 nt) | ydiL → | DUF1870 domain‑containing protein YdiL |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,770,851 | 0 | T | . | 94.6% | 209.5 / 8.0 | 56 | coding (237/357 nt) | ydiL | DUF1870 domain‑containing protein YdiL |
| Reads supporting (aligned to +/- strand): ref base T (1/2); new base . (24/29); total (25/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01 | |||||||||||
GGCGTCAGTGGGAAAATGGCAAGTGTGCCATTCCTGATCGTGTAGTCGAGCAACTGTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCAATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGCACACGATCTGGAGCGGCTTTGTTAATTTTTCCACAGAA > NC_000913/1770712‑1770984 | ggCGTCAGTGGGAAAATGGCAAGTGTGCCATTCCTGATCGTGTAGTCGAGCAACTGTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCGt > 1:326577/1‑140 (MQ=255) cGTCAGTGGGAAAATGGCAAGTGTGCCATTCCTGATCGTGTAGTCGAGCAACTGTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCGttt > 2:191167/1‑140 (MQ=255) tGGGAAAATGGCAAGTGTGCCATTCCTGATCGTGTAGTCGAGCAACTGTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCGTTTTTcccc < 2:326577/140‑1 (MQ=255) tGGCAAGTGTGCCATTCCTGATCGTGTAGTCGAGCAACTGTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCG‑TTTTTCCCCGACTTAAc < 1:134062/140‑1 (MQ=255) gCCATTCCTGATCGTGTAGTCGAGCAACTGTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCG‑TTTTTCCCCGACTTAACCGCATTTCaa < 2:152617/140‑1 (MQ=255) gCCATTCCTGATCGTGTAGTCGAGCAACTGTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCG‑TTTTTCCCCGACTTAACCGCATTTCaa < 1:191167/140‑1 (MQ=255) gCCATTCCTGATCGTGTAGTCGAGCAACTGTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCG‑TTTTTCCCCGACTTAACCGCATTTCaa < 1:674864/140‑1 (MQ=255) gCCATTCCTGATCGTGTAGTCGAGCAACTGTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCG‑TTTTTCCCCGACTTAACCGCATTTCaa < 2:107382/140‑1 (MQ=255) gCCATTCCTGATCGTGTAGTCGAGCAACTGTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCG‑TTTTTCCCCGACTTAACCGCATTTCaa < 2:155970/140‑1 (MQ=255) ccTGATCGTGTAGTCGAGCAACTGTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCG‑TTTTTCCCCGACTTAACCGCATTTCAACGAGTc > 2:41623/1‑140 (MQ=255) cTGATCGTGTAGTCGAGCAACTGTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCG‑TTTTTCCCCGACTTAACCGCATTTCAACGAGTCt > 1:727084/1‑140 (MQ=255) cTGATCGTGTAGTCGAGCAACTGTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCG‑TTTTTCCCCGACTTAACCGCATTTCAACGAGTCt > 2:900178/1‑140 (MQ=255) cTGATCGTGTAGTCGAGCAACTGTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCAATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCG‑TTTTTCCCCGACTTAACCGCATTTCAACGAGTCt > 2:1033845/1‑140 (MQ=255) gATCGTGTAGTCGAGCAACTGTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTa > 1:251126/1‑140 (MQ=255) gATCGTGTAGTCGAGCAACTGTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCG‑TTTTTCCCCGACTTAACCGCATTTCAACGAGTCTAc > 2:964977/1‑140 (MQ=255) aGTCGAGCAACTGTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCG‑TTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATgg < 2:159241/140‑1 (MQ=255) aGCAACTGTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCG‑TTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCAAtt > 2:1107380/1‑140 (MQ=255) aGCAACTGTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCG‑TTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCAAtt > 1:279770/1‑140 (MQ=255) aGCAACTGTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCG‑TTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCAAtt > 1:712567/1‑140 (MQ=255) cAACTGTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCG‑TTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCAAtttt > 2:713230/1‑140 (MQ=255) tGTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCAATTTTAtt < 2:251126/140‑1 (MQ=255) tGTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCG‑TTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCAATTTTATTg < 2:659596/140‑1 (MQ=255) gTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCG‑TTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCAATTTTATTGa > 1:886033/1‑140 (MQ=255) gTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCG‑TTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCAATTTTATTGa < 2:727084/140‑1 (MQ=255) ggCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCG‑TTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCAATTTTATTGACTg > 1:479763/1‑140 (MQ=255) ggCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCG‑CTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCAATTTTATTGACTg > 1:642215/1‑140 (MQ=255) cTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCG‑TTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCAATTTTATTGACTGGa < 2:733983/140‑1 (MQ=255) tATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCG‑TTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCAATTTTATTGACTGGaa > 2:278506/1‑140 (MQ=255) tATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCG‑TTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCAATTTTATTGACTGGaa < 1:860006/140‑1 (MQ=255) tGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCG‑TTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCAATTTTAtt > 2:1004725/1‑130 (MQ=255) tGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCG‑TTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCAATTTTAtt < 1:1004725/130‑1 (MQ=255) tGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCG‑TTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCAATTTTATTGACTGGAAGa > 1:998668/1‑140 (MQ=255) cAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCG‑TTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCAATTTTATTGACTGGAAGATCTAt < 1:964977/140‑1 (MQ=255) aGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCG‑TTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCAATTTTATTGACTGGAAGATCTATc > 2:803269/1‑140 (MQ=255) cAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCG‑TTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCAATTTTATTGACTGGAAGATCTATCaa > 2:316844/1‑140 (MQ=255) aaaaaaaCATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCG‑TTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCAATTTTATTGACTGGAAGATCTATCAATCGGt < 2:73910/140‑1 (MQ=255) aaaaaCATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCG‑TTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCAATTTTATTGACTGGAAGATCTATCAATCGGTAg < 2:886033/140‑1 (MQ=255) aaaaaCATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCG‑TTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCAATTTTATTGACTGGAAGATCTATCAATCGGTAg < 2:998668/140‑1 (MQ=255) cTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCG‑TTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCAATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGaa > 2:995932/1‑140 (MQ=255) cTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCG‑TTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCAATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGaa < 2:279770/140‑1 (MQ=255) cATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCG‑TTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCAATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATta > 2:150057/1‑140 (MQ=255) aTGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCG‑TTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCAATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATtat < 1:1107380/140‑1 (MQ=255) catcatAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCG‑TTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCAATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGc < 1:900178/140‑1 (MQ=255) catcatAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCG‑TTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCAATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGc < 1:484888/140‑1 (MQ=255) tcatAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCG‑TTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCAATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGcac < 2:479763/140‑1 (MQ=255) tcatAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCG‑TTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCAATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGcac < 1:470458/140‑1 (MQ=255) catAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCG‑TTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCAATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGcaca < 1:803269/140‑1 (MQ=255) catAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCG‑TTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCAATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGcaca < 1:316844/140‑1 (MQ=255) catAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCG‑TTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCAATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGcaca < 2:712567/140‑1 (MQ=255) catAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCG‑TTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCAATTTTATCGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGcaca > 2:708729/1‑140 (MQ=255) gagaAAATAAATAACCGCATCGGCAACAACACCATGCG‑CTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCAATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGCACACGAt < 2:642215/140‑1 (MQ=255) agaAAATAAATAACCGCATCGGCAACAACACCATGCG‑TTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCAATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGCACACGATc > 1:236356/1‑140 (MQ=255) agaAAATAAATAACCGCATCGGCAACAACACCATGCG‑TTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCAATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGCACACGATc > 1:1048892/1‑140 (MQ=255) aataaataACCGCATCGGCAACAACACCATGCG‑TTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCAATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGCACACGATCTGGa > 2:1062923/1‑140 (MQ=255) aaataaCCGCATCGGCAACAACACCATGCG‑TTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCAATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGCACACGATCTGGAGCg < 2:677118/140‑1 (MQ=255) taaCCGCATCGGCAACAACACCATGCG‑TTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCAATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGCACACGATCTGGAGCGGCt < 1:401376/140‑1 (MQ=255) acacCATGCG‑TTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCAATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGCACACGATCTGGAGCGGCTTTGTTAATTTTTCcaca > 1:803868/1‑140 (MQ=255) ccATGCG‑TTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCAATTTTATCGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGCACACGATCTGGAGCGGCTTTGTTAATTTTTCCACAGaa < 1:708729/140‑1 (MQ=255) | GGCGTCAGTGGGAAAATGGCAAGTGTGCCATTCCTGATCGTGTAGTCGAGCAACTGTTGGCTATGCGTCAGCAAAGAAAAAAACATCTTCATGCCATCATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCAATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGCACACGATCTGGAGCGGCTTTGTTAATTTTTCCACAGAA > NC_000913/1770712‑1770984 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |