Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	766,957	(G)6→7	coding (974/1977 nt)	mngA →	2‑O‑alpha‑mannosyl‑D‑glycerate specific PTS enzyme II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	766,951	1	.	G	100.0%	154.9 / NA	43	A323G (GCG→GGG)	mngA	2‑O‑alpha‑mannosyl‑D‑glycerate specific PTS enzyme II
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (22/21);  total (22/21)

GCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGC‑GGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAGCTGGGCGGCGGGCTGCTCGGAATTTTGA  >  NC_000913/766821‑767079                                                                                                                                    |                                                                                                                                 gcacTTACCCTCAAGCGTAGCGATGAGACGTGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGC‑gggggggca                                                                                                                         <  2:710203/137‑4 (MQ=255)  cgcTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGC‑gggggggcac                                                                                                                        >  1:1047882/1‑136 (MQ=255)  cgcTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGC‑gggggggcac                                                                                                                        >  2:176278/1‑136 (MQ=255)    cTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACgg                                                                                                                       >  2:208337/1‑140 (MQ=255)            cAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCg                                                                                                               >  1:1142551/1‑140 (MQ=255)                   aGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCgg                                                                                                        >  2:166677/1‑140 (MQ=255)                         gagaCGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTAc                                                                                                  <  1:93796/140‑1 (MQ=255)                            aCGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGGCCCGGGATTACttg                                                                                               >  2:93796/1‑138 (MQ=255)                               cgtaccgtacAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGc                                                                                            >  1:392453/1‑140 (MQ=255)                               cgtaccgtacAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGc                                                                                            >  1:596792/1‑140 (MQ=255)                               cgtaccgtacAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGc                                                                                            >  2:176699/1‑140 (MQ=255)                                      tacAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCtt                                                                                     <  2:417668/140‑1 (MQ=255)                                      tacAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCtt                                                                                     >  2:373623/1‑140 (MQ=255)                                      tacAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCtt                                                                                     <  2:1047882/140‑1 (MQ=255)                                        cAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCg                                                                                   <  1:1141742/140‑1 (MQ=255)                                         aGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCgg                                                                                  <  1:408254/140‑1 (MQ=255)                                         aGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCgg                                                                                  <  1:1121633/140‑1 (MQ=255)                                         aGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCgg                                                                                  <  2:169604/140‑1 (MQ=255)                                           cAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGc                                                                                >  1:187736/1‑140 (MQ=255)                                              gATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTAc                                                                             <  1:208337/140‑1 (MQ=255)                                              gATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTAc                                                                             <  2:187736/140‑1 (MQ=255)                                                tACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACaa                                                                           >  2:583950/1‑140 (MQ=255)                                                  cGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGa                                                                         <  1:176278/140‑1 (MQ=255)                                                   gCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACaa                                                                           <  2:5059/137‑1 (MQ=255)                                                   gCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACaa                                                                           >  1:5059/1‑137 (MQ=255)                                                   gCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGAt                                                                        <  2:596792/140‑1 (MQ=255)                                                   gCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGGATTACTGTCGCAAATCTTCGGGCTACAAGAt                                                                        >  2:520608/1‑140 (MQ=255)                                                     aaCCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGGATTACTGTCGCAAATCTTCGGGCTACAAGATCt                                                                      >  2:638143/1‑140 (MQ=255)                                                          gTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTa                                                                 <  2:392453/140‑1 (MQ=255)                                                          gTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTa                                                                 >  1:1044799/1‑140 (MQ=255)                                                                    tCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGaaaa                                                       >  2:9291/1‑140 (MQ=255)                                                                       aaaCGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTc                                                    <  2:1044799/140‑1 (MQ=255)                                                                         aCGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCt                                                  <  2:1142551/140‑1 (MQ=255)                                                                            gAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGc                                               >  1:1116849/1‑140 (MQ=255)                                                                            gAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGc                                               <  2:625548/140‑1 (MQ=255)                                                                            gAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGc                                               >  1:743920/1‑140 (MQ=255)                                                                            gAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGT‑‑CAAATCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCtg                                             >  2:1018592/1‑140 (MQ=255)                                                                             aGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCt                                              <  1:507255/140‑1 (MQ=255)                                                                               cTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCtgt                                            <  1:583950/140‑1 (MQ=255)                                                                                       ggCACTGTTGAGCGGAATCTCTTTTGCCGTACCGCTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTAc                                    >  2:183596/1‑140 (MQ=255)                                                                                        gCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTAcc                                   <  2:273329/140‑1 (MQ=255)                                                                                          aCTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGc                                 >  2:523131/1‑140 (MQ=255)                                                                                                 aGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAGCTgg                          >  1:414413/1‑140 (MQ=255)                                                                                                               tGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAGCTGGGCGGCGGGCTGCTc            <  1:9291/140‑1 (MQ=255)                                                                                                                gCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAGCTGGGCGGCGGGCTGCTCg           >  1:164373/1‑140 (MQ=255)                                                                                                                         ttGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAGCTGGGCGGCGGGCTGCTCGGAATTTTGa  <  1:69838/140‑1 (MQ=255)                                                                                                                         ttGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGCATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAGCTGGGCGGCGGGCTGCTCGGAATTTTGa  <  2:752638/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                 GCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGC‑GGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAGCTGGGCGGCGGGCTGCTCGGAATTTTGA  >  NC_000913/766821‑767079

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑