Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	893,874	(A)6→7	coding (91/1113 nt)	potF →	putrescine ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	893,868	1	.	A	100.0%	159.8 / NA	45	Q29K (CAA→AAA)	potF	putrescine ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (20/25);  total (20/25)

TTCAGTGTGACAACTTTTGTTCGTTTGTTAACGAACTTTCAGAAGGAAAGAGATATGACCGCCTTAAATAAAAAATGGCTATCGGGTCTGGTTGCGGGTGCTCTGATGGCCGTCTCTGTCGGCACGCTCGCGGCTGAAC‑AAAAAACACTCCACATTTATAACTGGTCTGATTATATCGCCCCGGACACGGTGGCCAATTTTGAAAAAGAAACCGGTATTAAAGTCGTCTACGATGTTTTCGACTCTAACGAAGTACTGGAAGGCAAATTA  >  NC_000913/893730‑893999                                                                                                                                            |                                                                                                                                    ttCAGTGTGACAACTTTTGTTCGTTTGTTAACGAACTTTCAGAAGGAAAGAGATATGACCGCCTTAAATAAAAAATGGCTATCGGGTCTGGTTGCGGGTGCTCTGATGGCCGTCTCTGTCGGCACGCTCGCGGCTGAAC‑a                                                                                                                                    <  1:159529/140‑1 (MQ=255)  tCAGTGTGACAACTTTTGTTCGTTTGTTAACGAACTTTCAGAAGGAAAGAGATATGACCGCCTTAAATAAAAAATGGCTATCGGGTCTGGTTGCGGGTGCTCTGATGGCCGTCTCTGTCGGCACGCTCGCGGCTGAAC‑aa                                                                                                                                   >  1:2508/1‑140 (MQ=255)         gACAACTTTTGTTCGTTTGTTAACGAACTTTCAGAAGGAAAGAGATATGACCGCCTTAAATAAAAAATGGCTATCGGGTCTGGTTGCGGGTGCTCTGATGGCCGTCTCTGTCGGCACGCTCGCGGCTGAAC‑aaaaaaaca                                                                                                                            <  2:838755/140‑4 (MQ=255)         gACAACTTTTGTTCGTTTGTTAACGAACTTTCAGAAGGAAAGAGATATGACCGCCTTAAATAAAAAATGGCTATCGGGTCTGGTTGCGGGTGCTCTGATGGCCGTCTCTGTCGGCACGCTCGCGGCTGAAC‑aaaaaaaca                                                                                                                            >  1:838755/1‑137 (MQ=255)          aCAACTTTTGTTCGTTTGTTAACGAACTTTCAGAAGGAAAGAGATATGACCGCCTTAAATAAAAAATGGCTATCGGGTCTGGTTGCGGGTGCTCTGATGGCCGTCTCTGTCGGCACGCTCGCGGCTGAAC‑aaaaaaacac                                                                                                                           >  1:141532/1‑136 (MQ=255)             aCTTTTGTTCGTTTGTTAACGAACTTTCAGAAGGAAAGAGATATGACCGCCTTAAATAAAAAATGGCTATCGGGTCTGGTTGCGGGTGCTCTGATGGCCGTCTCTGTCGGCACGCTCGCGGCTGAACAAAAAAACACTcc                                                                                                                         <  2:945420/140‑1 (MQ=255)             aCTTTTGTTCGTTTGTTAACGAACTTTCAGAAGGAAAGAGATATGACCGCCTTAAATAAAAAATGGCTATCGGGTCTGGTTGCGGGTGCTCTGATGGCCGTCTCTGTCGGCACGCTCGCGGCTGAACAAAAAAACACTcc                                                                                                                         <  2:620553/140‑1 (MQ=255)             aCTTTTGTTCGTTTGTTAACGAACTTTCAGAAGGAAAGAGATATGACCGCCTTAAATAAAAAATGGCTATCGGGTCTGGTTGCGGGTGCTCTGATGGCCGTCTCTGTCGGCACGCTCGCGGCTGAACAAAAAAACACTcc                                                                                                                         <  1:868377/140‑1 (MQ=255)             aCTTTTGTTCGTTTGTTAACGAACTTTCAGAAGGAAAGAGATATGACCGCCTTAAATAAAAAATGGCTATCGGGTCTGGTTGCGGGTGCTCTGATGGCCGTCTCTGTCGGCACGCTCGCGGCTGAACAAAAAAACACTcc                                                                                                                         <  1:608095/140‑1 (MQ=255)              cTTTTGTTCGTTTGTTAACGAACTTTCAGAAGGAAAGAGATATGACCGCCTTAAATAAAAAATGGCTATCGGGTCTGGTTGCGGGTGCTCTGATGGCCGTCTCTGTCGGCACGCTCGCGGCTGAACAAAAAAACACTCca                                                                                                                        <  1:13892/140‑1 (MQ=255)              cTTTTGTTCGTTTGTTAACGAACTTTCAGAAGGAAAGAGATATGACCGCCTTAAATAAAAAATGGCTATCGGGTCTGGTTGCGGGTGCTCTGATGGCCGTCTCTGTCGGCACGCTCGCGGCTGAACAAAAAAACACTCca                                                                                                                        <  2:72599/140‑1 (MQ=255)                  tGTTCGTTTGTTAACGAACTTTCAGAAGGAAAGAGATATGACCGCCTTAAATAAAAAATGGCTATCGGGTCTGGTTGCGGGTGCTCTGATGGCCGTCTCTGTCGGCACGCTCGCGGCTGAACAAAAAAACACTc                                                                                                                          <  1:1155523/134‑1 (MQ=255)                  tGTTCGTTTGTTAACGAACTTTCAGAAGGAAAGAGATATGACCGCCTTAAATAAAAAATGGCTATCGGGTCTGGTTGCGGGTGCTCTGATGGCCGTCTCTGTCGGCACGCTCGCGGCTGAACAAAAAAACACTc                                                                                                                          >  2:1155523/1‑134 (MQ=255)                        tttGTTAACGAACTTTCAGAAGGAAAGAGATATGACCGCCTTAAATAAAAAATGGCTATCGGGTCTGGTTGCGGGTGCTCTGATGGCCGTCTCTGTCGGCACGCTCGCGGCTGAACAAAAAAACACTCCACAtt                                                                                                                    <  1:516713/134‑1 (MQ=255)                        tttGTTAACGAACTTTCAGAAGGAAAGAGATATGACCGCCTTAAATAAAAAATGGCTATCGGGTCTGGTTGCGGGTGCTCTGATGGCCGTCTCTGTCGGCACGCTCGCGGCTGAACAAAAAAACACTCCACAtt                                                                                                                    >  2:516713/1‑134 (MQ=255)                        tttGTTAACGAACTTTCAGAAGGAAAGAGATATGACCGCCTTAAATAAAAAATGGCTATCGGGTCTGGTTGCGGGTGCTCTGATGGCCGTCTCTGTCGGCACGCTCGCGGCTGAACAAAAAAACACTCCACATTTATAAc                                                                                                              >  1:673841/1‑140 (MQ=255)                         ttGTTAACGAACTTTCAGAAGGAAAGAGATATGACCGCCTTAAATAAAAAATGGCTATCGGGTCTGGTTGCGGGTGCTCTGATGGCCGTCTCTGTCGGCACGCTCGCGGCTGAAC‑aaaaaaacac                                                                                                                           >  2:777405/1‑121 (MQ=255)                         ttGTTAACGAACTTTCAGAAGGAAAGAGATATGACCGCCTTAAATAAAAAATGGCTATCGGGTCTGGTTGCGGGTGCTCTGATGGCCGTCTCTGTCGGCACGCTCGCGGCTGAAC‑aaaaaaacac                                                                                                                           <  1:777405/125‑5 (MQ=255)                                   aCTTTCAGAAGGAAAGAGATATGACCGCCTTAAATAAAAAATGGCTATCGGGTCTGGTTGCGGGTGCTCTGATGGCCGTCTCTGTCGGCACGCTCGCGGCTGAACAAAAAAACACTCCACATTTATAACTGGTCTGATta                                                                                                   >  1:142192/1‑140 (MQ=255)                                     tttCAGAAGGAAAGAGATATGACCGCCTTAAATAAAAAATGGCTATCGGGTCTGGTTGCGGGTGCTCTGATGGCCGTCTCTGTCGGCACGCTCGCGGCTGAACAAAAAAACACTCCACATTTATAACTGGTCTGATtata                                                                                                 <  2:673841/140‑1 (MQ=255)                                                 agagATATGACCGCCTTAAATAAAAAATGGCTATCGGGTCTGGTTGCGGGTGCTCTGATGGCCGTCTCTGTCGGCACGCTCGCGGCTGAACAAAAAAACACTCCACATTTATAACTGGTCTGATTATATCGCCCCGGaca                                                                                     <  2:142192/140‑1 (MQ=255)                                                   agaTATGACCGCCTTAAATAAAAAATGGCTATCGGGTCTGGTTGCGGGTGCTCTGATGGCCGTCTCTGTCGGCACGCTCGCGGCTGAACAAAAAAACACTCCACATTTATAACTGGTCTGATTATATCGCCCCGGACACg                                                                                   <  2:739734/140‑1 (MQ=255)                                                        tGACCGCCTTAAATAAAAAATGGCTATCGGGTCTGGTTGCGGGTGCTCTGATGGCCGTCTCTGTCGGCACGCTCGCGGCTGAACAAAAAAACACTCCACATTTATAACTGGTCTGATTATATCGcc                                                                                            >  2:377118/1‑126 (MQ=255)                                                        tGACCGCCTTAAATAAAAAATGGCTATCGGGTCTGGTTGCGGGTGCTCTGATGGCCGTCTCTGTCGGCACGCTCGCGGCTGAACAAAAAAACACTCCACATTTATAACTGGTCTGATTATATCGcc                                                                                            <  1:377118/126‑1 (MQ=255)                                                          aCCGCCTTAAATAAAAAATGGCTATCGGGTCTGGTTGCGGGTGCTCTGATGGCCGTCTCTGTCGGCACGCTCGCGGCTGAACAAAAAAACACTCCACATTTATAACTGGTCTGATTATATCGCCCCGGACACGGTGGCCa                                                                            >  2:283137/1‑140 (MQ=255)                                                                         aaaTGGCTATCGGGTCTGGTTGCGGGTGCTCTGATGGCCGTCTCTGTCGGCACGCTCGCGGCTGAACAAAAAAACACTCCACATTTATAACTGGTCTGATTATATCGCCCCGGACACGGTGGCCAATTTTGAAAAAGaaa                                                             >  1:683057/1‑140 (MQ=255)                                                                          aaTGGCTATCGGGTCTGGTTGCGGGTGCTCTGATGGCCGTCTCTGTCGGCACGCTCGCGGCTGAACAAAAAAACACTCCACATTTATAACTGGTCTGATTATATCGCCCCGGACACGGTGGCCAATTTTGAAAAAGAAAc                                                            <  1:816745/140‑1 (MQ=255)                                                                          aaTGGCTATCGGGTCTGGTTGCGGGTGCTCTGATGGCCGTCTCTGTCGGCACGCTCGCGGCTGAACAAAAAAACACTCCACATTTATAACTGGTCTGATTATATCGCCCCGGACACGGTGGCCAATTTTGAAAAAGAAAc                                                            <  2:2508/140‑1 (MQ=255)                                                                           aTGGCTATCGGGTCTGGTTGCGGGTGCTCTGATGGCCGTCTCTGTCGGCACGCTCGCGGCTGAACAAAAAAACACTCCACATTTATAACTGGTCTGATTATATCGCCCCGGACACGGTGGCCAATTTTGAAAAAGAAAcc                                                           <  1:459932/140‑1 (MQ=255)                                                                                 aTCGGGTCTGGTTGCGGGTGCTCTGATGGCCGTCTCTGTCGGCACGCTCGCGGCTGAACAAAAAAACACTCCACATTTATAACTGGTCTGATTATATCGCCCCGGACACGGTGGCCAATTTTGAAAAAGAAACCGGTAtt                                                     >  1:652945/1‑140 (MQ=255)                                                                                    gggTCTGGTTGCGGGTGCTCTGATGGCCGTCTCTGTCGGCACGCTCGCGGCTGAACAAAAAAACACTCCACATTTATAACTGGTCTGATTATATCGCCCCGGACACGGTGGCCAATTTTGAAAAAGAAACCGGTATTaaa                                                  <  2:216275/140‑1 (MQ=255)                                                                                    gggTCTGGTTGCGGGTGCTCTGATGGCCGTCTCTGTCGGCACGCTCGCGGCTGAACAAAAAAACACTCCACATTTATAACTGGTCTGATTATATCGCCCCGGACACGGTGGCCAATTTTGAAAAAGAAACCGGTATTaaa                                                  <  2:406278/140‑1 (MQ=255)                                                                                       tCTGGTTGCGGGTGCTCTGATGGCCGTCTCTGTCGGCACGCTCGCGGCTGAACAAAAAAACACTCCACATTTATAACTGGTCTGATTATATCGCCCCGGACACGGTGGCCAATTTTGAAAAAGAAACCGGTATTAAAgtc                                               >  2:690722/1‑140 (MQ=255)                                                                                               cGGGTGCTCTGATGGCCGTCTCTGTCGGCACGCTCGCGGCTGAACAAAAAAACACTCCACATTTATAACTGGTCTGATTATATCGCCCCGGACACGGTGGCCAATTTTGAAAAAGAAACCGGTATTAAAGTCGTCTACGa                                       >  2:1147284/1‑140 (MQ=255)                                                                                                gggTGCTCTGATGGCCGTCTCTGTCGGCACGCTCGCGGCTGAACAAAAAAACACTCCACATTTATAACTGGTCTGATTATATCGCCCCGGACACGGTGGCCAATTTTGAAAAAGAAACCGGTATTAAAGTCGTCTACGAt                                      >  1:33031/1‑140 (MQ=255)                                                                                                 ggTGCTCTGATGGCCGTCTCTGTCGGCACGCTCGCGGCTGAACAAAAAAACACTCCACATTTATAACTGGTCTGATTATATCGCCCCGGACACGGTGGCCAATTTTGAAAAAGAAACCGGTATTAAAGTCGTCTACGATg                                     >  1:698615/1‑140 (MQ=255)                                                                                                     ctctGATGGCCGTCTCTGTCGGCACGCTCGCGGCTGAACAAAAAAACACTCCACATTTATAACTGGTCTGATTATATCGCCCCGGACACGGTGGCCAATTTTGAAAAAGAAACCGGTATTAAAGTCGTCTACGATGtttt                                 >  1:237623/1‑140 (MQ=255)                                                                                                     ctctGATGGCCGTCTCTGTCGGCACGCTCGCGGCTGAACAAAAAAACACTCCACATTTATAACTGGTCTGATTATATCGCCCCGGACACGGTGGCCAATTTTGAAAAAGAAACCGGTATTAAAGTCGTCTACGATGtttt                                 >  1:344106/1‑140 (MQ=255)                                                                                                     ctctGATGGCCGTCTCTGTCGGCACGCTCGCGGCTGAACAAAAAAACACTCCACATTTATAACTGGTCTGATTATATCGCCCCGGACACGGTGGCCAATTTTGAAAAAGAAACCGGTATTAAAGTCGTCTACGATGtttt                                 >  2:886081/1‑140 (MQ=255)                                                                                                      tctGATGGCCGTCTCTGTCGGCACGCTCGCGGCTGAACAAAAAAACACTCCACATTTATAACTGGTCTGATTATATCGCCCCGGACACGGTGGCCAATTTTGAAAAAGAAACCGGTATTAAAg                                                 <  2:821822/123‑1 (MQ=255)                                                                                                      tctGATGGCCGTCTCTGTCGGCACGCTCGCGGCTGAACAAAAAAACACTCCACATTTATAACTGGTCTGATTATATCGCCCCGGACACGGTGGCCAATTTTGAAAAAGAAACCGGTATTAAAg                                                 >  1:821822/1‑123 (MQ=255)                                                                                                      tctGATGGCCGTCTCTGTCGGCACGCTCGCGGCTGAACAAAAAAACACTCCACATTTATAACTGGTCTGATTATATCGCCCCGGACACGGTGGCCAATTTTGAAAAAGAAACCGGTATTAAAGTCGTCTACGATGTTTTc                                <  1:690722/140‑1 (MQ=255)                                                                                                        tGATGGCCGTCTCTGTCGGCACGCTCGCGGCTGAACAAAAAAACACTCCACATTTATAACTGGTCTGATTATATCGCCCCGGACACGGTGGCCAATTTTGAAAAAGAAACCGGTATTAAAGTCGTCTACGATGTTTTCGa                              <  2:652945/140‑1 (MQ=255)                                                                                                               cGTCTCTGTCGGCACGCTCGCGGCTGAACAAAAAAACACTCCACATTTATAACTGGTCTGATTATATCGCCCCGGACACGGTGGCCAATTTTGAAAAAGAAACCGGTATTAAAGTCGTCTACGATGTTTTCGACTCTAAc                       >  1:365776/1‑140 (MQ=255)                                                                                                               cGTCTCTGTCGGCACGCTCGCGGCTGAACAAAAAAACACTCCACATTTATAACTGGTCTGATTATATCGCCCCGGACACGGTGGCCAATTTTGAAAAAGAAACCGGTATTAAAGTCGTCTACGATGTTTTCGACTCTAAc                       >  1:233779/1‑140 (MQ=255)                                                                                                                gTCTCTGTCGGCACGCTCGCGGCTGAACAAAAAAACACTCCACATTTATAACTGGTCTGATTATATCGCCCCGGACACGGTGGCCAATTTTGAAAAAGAAACCGGTATTAAAGTCGTCTACGATGTTTTCGACTCTAACg                      <  2:365776/140‑1 (MQ=255)                                                                                                                gTCTCTGTCGGCACGCTCGCGGCTGAACAAAAAAACACTCCACATTTATAACTGGTCTGATTATATCGCCCCGGACACGGTGGCCAATTTTGAAAAAGAAACCGGTATTAAAGTCGTCTACGATGTTTTCGACTCTAACg                      <  2:344106/140‑1 (MQ=255)                                                                                                                          gCACGCTCGCGGCTGAACAAAAAAACACTCCACATTTATAACTGGTCTGATTATATCGCCCCGGACACGGTGGCCAATTTTGAAAAAGAAACCGGTATTAAAGTCGTCTACGATGTTTTCGACTCTAACGAAGTACTGGa            <  1:283137/140‑1 (MQ=255)                                                                                                                          gCACGCTCGCGGCTGAACAAAAAAACACTCCACATTTATAACTGGTCTGATTATATCGCCCCGGACACGGTGGCCAATTTTGAAAAAGAAACCGGTATTAAAGTCGTCTACGATGTTTTCGACTCTAACGAAGTACTGGa            <  1:1147284/140‑1 (MQ=255)                                                                                                                           cACGCTCGCGGCTGAACAAAAAAACACTCCACATTTATAACTGGTCTGATTATATCGCCCCGGACACGGTGGCCAATTTTGAAAAAGAAACCGGTATTAAAGTCGTCTACGATGTTTTCGACTCTAACGAAGTACTGGaa           >  1:293/1‑140 (MQ=255)                                                                                                                               cTCGCGGCTGAACAAAAAAACACTCCACATTTATAACTGGTCTGATTATATCGCCCCGGACACGGTGGCCAATTTTGAAAAAGAAACCGGTATTAAAGTCGTCTACGATGTTTTCGACTCTAACGAAGTACTGGAAGGCa       <  2:33031/140‑1 (MQ=255)                                                                                                                               cTCGCGGCTGAACAAAAAAACACTCCACATTTATAACTGGTCTGATTATATCGCCCCGGACACGGTGGCCAATTTTGAAAAAGAAACCGGTATTAAAGTCGTCTACGATGTTTTCGACTCTAACGAAGTACTGGAAGGCa       >  2:896708/1‑140 (MQ=255)                                                                                                                                    ggCTGAACAAAAAAACACTCCACATTTATAACTGGTCTGATTATATCGCCCCGGACACGGTGGCCAATTTTGAAAAAGAAACCGGTATTAAAGTCGTCTACGATGTTTTCGACTCTAACGAAGTACTGGAAGGCAAATTa  <  1:347112/140‑1 (MQ=255)                                                                                                                                            |                                                                                                                                    TTCAGTGTGACAACTTTTGTTCGTTTGTTAACGAACTTTCAGAAGGAAAGAGATATGACCGCCTTAAATAAAAAATGGCTATCGGGTCTGGTTGCGGGTGCTCTGATGGCCGTCTCTGTCGGCACGCTCGCGGCTGAAC‑AAAAAACACTCCACATTTATAACTGGTCTGATTATATCGCCCCGGACACGGTGGCCAATTTTGAAAAAGAAACCGGTATTAAAGTCGTCTACGATGTTTTCGACTCTAACGAAGTACTGGAAGGCAAATTA  >  NC_000913/893730‑893999

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑