Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,585,101	(C)6→7	coding (1386/2280 nt)	ydeP ←	putative oxidoreductase YdeP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,585,095	1	.	C	100.0%	179.3 / NA	50	G464G (GGC→GGG)	ydeP	putative oxidoreductase YdeP
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (22/28);  total (22/28)

CCAGGACCGGCAGAATATAGCTATGCCGTGCGGTCAACAGATGAGAGCGGTTAAGCTTAGTGGCTACGTGTACCGCCAAATCTAATTGCGTTAACGGTACAGCGCTCGCTTCCCGATCTGGCATTGCCAGCGCAAAATTG‑‑CCCCCCATGCAGATCAATGCTCGAGCCTGCCCCGTACATATTGCTTGCATGCTGGCAATTGCAGCATGTCCAGGTGCATGAGGTGGGGTGAAGCCATAGCGCTCACCCAGACGAGCCAGAA  >  NC_000913/1584956‑1585216                                                                                                                                             |                                                                                                                           ccAGGACCGGCAGAATATAGCTATGCCGTGCGGTCAACAGATGAGAGCGGTTAAGCTTAGTGGCTACGTGTACCGCCAAATCTAATTGCGTTAACGGTACAGCGCTCGCTTCCCGATCTGGCATTGCCAGCGCAAAATTg                                                                                                                             >  1:1090140/1‑140 (MQ=255)     gACCGGCAGAATATAGCTATGCCGTGCGGTCAACAGATGAGAGCGGTTAAGCTTAGTGGCTACGTGTACCGCCAAATCTAATTGCGTTAACGGTACAGCGCTCGCTTCCCGATCTGGCATTGCCAGCGCAAAATTG‑‑cccc                                                                                                                       <  1:287647/140‑1 (MQ=255)      gccGGCAGAATCTAGCTATGCCGTGCGGTCAAAAGATGAGAGCGGTTAAGCTTAGTGGCTCCGTGTACCGCCAAAACTAATTGCCTTAACGGTACAGCGCTCGCTTCCCGATCTGGCACTGCCAGCGCAAAATTG‑‑ccccc                                                                                                                      <  2:463116/139‑1 (MQ=255)      aCCGGCAGAATATAGCTATGCCGTGCGGTCAACAGATGAGAGCGGTTAAGCTTAGTGGCTACGTGTACCGCCAAATCTAATTGCGTTAACGGTACAGCGCTCGCTTCCCGATCTGGCATTGCCAGCGCAAAATTG‑‑ccccc                                                                                                                      <  1:622471/140‑1 (MQ=255)      aCCGGCAGAATATAGCTATGCCGTGCGGTCAACAGATGAGAGCGGTTAAGCTTAGTGGCTACGTGTACCGCCAAATCTAATTGCGTTAACGGTACAGCGCTCGCTTCCCGATCTGGCATTGCCAGCGCAAAATTG‑‑ccccc                                                                                                                      <  2:639524/140‑1 (MQ=255)      aCCGGCAAAATATAGCTATGCCGTGCGGTCAACAGATGAGAGCGGTTAAGCTTAGTGGCTACGTGTACCGCCAAATCTAATTGCGTTAACGGTACAGCGCTCGCTTCCCGATCTGGCATTGCCAGCGCAAAATTG‑‑ccccc                                                                                                                      <  2:557500/140‑1 (MQ=255)        cGGCAGAATATAGCTATGCCGTGCGGTCAACAGATGAGAGCGGTTAAGCTTAGTGGCTACGTGTACCGCCAAATCTAATTGCGTTAACGGTACAGCGCTCGCTTCCCGATCTGGCATTGCCAGCGCAAAATTG‑‑ccccccc                                                                                                                    <  1:742034/140‑2 (MQ=255)          gCAGAATATAGCTATGCCGTGCGGTCAACAGATGAGAGCGGTTAAGCTTAGTGGCTACGTGTACCGCCAAATCTAATTGCGTTAACGGTACAGCGCTCGCTTCCCGATCTGGCATTGCCAGCGCAAAATTG‑‑cccccccat                                                                                                                  >  2:846888/1‑137 (MQ=255)          gCAGAATATAGCTATGCCGTGCGGTCAACAGATGAGAGCGGTTAAGCTTAGTGGCTACGTGTACCGCCAAATCTAATTGCGTTAACGGTACAGCGCTCGCTTCCCGATCTGGCATTGCCAGCGCAAAATTG‑‑cccccccat                                                                                                                  >  2:640343/1‑137 (MQ=255)                        tGCCGTGCGGTCAACAGATGAGAGCGGTTAAGCTTAGTGGCTACGTGTACCGCCAAATCTAATTGCGTTAACGGTACAGGGCTCGCTTCGCGATCTGGCATTGCCAGCGCAAAATTGC‑CCCCCCATGCAGATCAATGCTc                                                                                                     >  2:1036081/1‑140 (MQ=255)                        tGCCGTGCGGTCAACAGATGAGAGCGGTTAAGCTTAGTGGCTACGTGTACCGCCAAATCTAATTGCGTTAACGGTACAGCGCTCGCTTCCCGATCTGGCATTGCCAGCGCAAAATTGC‑CCCCCCATGCAGATCAATGCTc                                                                                                     >  1:764762/1‑140 (MQ=255)                        tGCCGTGCGGTCAACAGATGAGAGCGGTTAAGCTTAGTGGCTACGTGTACCGCCAAATCTAATTGCGTTAACGGTACAGCGCTCGCTTCCCGATCTGGCATTGCCAGCGCAAAATTGC‑CCCCCCATGCAGATCAATGCTc                                                                                                     >  2:822191/1‑140 (MQ=255)                            gTGCGGTCAACAGATGAGAGCGGTTAAGCTTAGTGGCTACGTGTACCGCCAAATCTAATTGCGTTAACGGTACAGCGCTCGCTTCCCGATCTGGCATTGCCAGCGCAAAATTGC‑CCCCCCATGCAGATCAATGCTCGAGc                                                                                                 <  1:950250/140‑1 (MQ=255)                             tGCGGTCAACAGATGAGAGCGGTTAAGCTTAGTGGCTACGTGTACCGCCAAATCTAATTGCGTTAACGGTACAGCGCTCGCTTCCCGATCTGGCATTGCCAGCGCAAAATTGC‑CCCCCCATGCAGATCAATGCTCGAGcc                                                                                                >  2:457223/1‑140 (MQ=255)                             tGCGGTCAACAGATGAGAGCGGTTAAGCTTAGTGGCTACGTGTACCGCCAAATCTAATTGCGTTAACGGTACAGCGCTCGCTTCCCGATCTGGCATTGCCAGCGCAAAATTGC‑CCCCCCATGCAGATCAATGCTCGAGcc                                                                                                >  2:1060209/1‑140 (MQ=255)                              gCGGTCAACAGATGAGAGCGGTTAAGCTTAGTGGCTACGTGTACCGCCAAATCTAATTGCGTTAACGGTACAGCGCTCGCTTCCCGATCTGGCATTGCCAGCGCAAAATTGC‑CCCCCCATGCAGATCAATGCTCGAGCCt                                                                                               <  2:240073/140‑1 (MQ=255)                               cGGTCAACAGATGAGAGCGGTTAAGCTTAGTGGCTACGTGTACCGCCAAATCTAATTGCGTTAACGGTACAGCGCTCGCTTCCCGATCTGGCATTGCCAGCGCAAAATTGC‑CCCCCCATGCAGATCAATGCTCGAGCCTg                                                                                              >  1:15171/1‑140 (MQ=255)                                  tCAACAGATGAGAGCGGTTAAGCTTAGTGGCTACATGTACCGCCAAATCTAATTGCGTTAACGGTACAGCGCTCGCTTCCCGATCTGGCATTGCCAGCGCAAAATTGC‑CCCCCCATGAAGATCAATGCt                                                                                                      <  1:227759/129‑1 (MQ=255)                                  tCAACAGATGAGAGCGGTTAAGCTTAGTGGCTACATGTACCGCCAAATCTAATTGCGTTAACGGTACAGCGCTCGCTTCCCGATCTGGCATTGCCAGCGCAAAATTGC‑CCCCCCATGAAGATCAATGCt                                                                                                      >  2:227759/1‑129 (MQ=255)                                    aaCAGATGAGAGCGGTTAAGCTTAGTGGCTACGTGTACCGCCAAATCTAATTGCGTTAACGGTACAGCGCTCGCTTCCCGATCTGGCATTGCCAGCGCAAAATTGC‑CCCCCCATGCAGATCAATGCTCGAGCCTGCCCCg                                                                                         <  1:457223/140‑1 (MQ=255)                                          tGAGAGCGGTTAAGCTTAGTGGCTACGTGTACCGCCAAATCTAATTGCGTTAACGGTACAGCGCTCGCTTCCCGATCTGGCATTGCCAGCGCAAAATTGC‑CCCCCCATGCAGATCAATGCTCGAGCCTGCCCCGTACata                                                                                   <  1:846888/140‑1 (MQ=255)                                            agagCGGTTAAGCTTAGTGGCTACGTGTACCGCCAAATCTAATTGCGTTAACGGTACAGCGCTCGCTTCCCGATCTGGCATTGCCAGCGCAAAATTGC‑CCCCCCATGCAGATCAATGCTCGAGCCTGCCCCGTACATAtt                                                                                 >  2:944477/1‑140 (MQ=255)                                            agagCGGTTAAGCTTAGTGGCTACGTGTACCGCCAAATCTAATTGCGTTAACGGTACAGCGCTCGCTTCCCGATCTGGCATTGCCAGCGCAAAATTGC‑CCCCCCATGCAGATCAATGCTCGAGCCTGCCCCGTACATAtt                                                                                 >  1:902281/1‑140 (MQ=255)                                              agCGGTTAAGCTTAGTGGCTACGTGTACCGCCAAATCTAATTGCGTTAACGGTACAGCGCTCGCTTCCCGATCTGGCATTGCCAGCGCAAAATTGC‑CCCCCCATGCAGATCAATGCTCGAGCCTGCCCCGTACATAttgc                                                                               >  2:837235/1‑140 (MQ=255)                                                   ttAAGCTTAGTGGCTACGTGTACCGCCAAATCTAATTGCGTTAACGGTACAGCGCTCGCTTCCCGATCTGGCATTGCCAGCGCAAAATTGC‑CCCCCCATGCAGATCAATGCTCGAGCCTGCCCCGTACATATTGCTTGCa                                                                          >  1:965122/1‑140 (MQ=255)                                                     aaGCTTAGTGGCTACGTGTACCGCCAAATCTAATTGCGTTAACGGTACAGCGCTCGCTTCCCGATCTGGCATTGCCAGCGCAAAATTGC‑CCCCCCATGCAGATCAATGCTCGAGCCTGCCCCGTACATATTGCTTGCATg                                                                        <  2:902281/140‑1 (MQ=255)                                                             tGGCTACGTGTACCGCCAAATCTAATTGCGTTAACGGTACAGCGCTCGCTTCCCGATCTGGCATTGCCAGCGCAAAATTGC‑CCCCCCATGCAGATCAATGCTCGAGCCTGCCCCGTACATATTGCTTGCATg                                                                        >  2:948425/1‑132 (MQ=255)                                                             tGGCTACGTGTACCGCCAAATCTAATTGCGTTAACGGTACAGCGCTCGCTTCCCGATCTGGCATTGCCAGCGCAAAATTGC‑CCCCCCATGCAGATCAATGCTCGAGCCTGCCCCGTACATATTGCTTGCATg                                                                        <  1:948425/132‑1 (MQ=255)                                                             tGGCTACGTGTACCGCCAAATCTAATTGCGTTAACGGTACAGCGCTCGCTTCCCGATCTGGCATTGCCAGCGCAAAATTGC‑CCCCCCATGCAGATCAATGCTCGAGCCTGCCCCGTACATATTGCTTGCATGCTGGCAAt                                                                >  1:178197/1‑140 (MQ=255)                                                                  aCGTGTACCGCCAAATCTAATTGCGTTAACGGTACAGCGCTCGCTTCCCGATCTGGCATTGCCAGCGCAAAATTGC‑CCCCCCATGCAGATCAATGCTCGAGCCTGCCCCGTACATATTGCTTGCATGCTGGCAATTgcag                                                           <  1:658388/140‑1 (MQ=255)                                                                   cGTGTACCGCCAAATCTAATTGCGTTAACGGTACAGCGCTCGCTTCCCGATCTGGCATTGCCAGCGCAAAATTGC‑CCCCCCATGCAGATCAATGCTCGAGCCTGCCCCGTACATATTGCTTGCATGCTGGCAATTgcagc                                                          <  1:1151491/140‑1 (MQ=255)                                                                   cGTGTACCGCCAAATCTAATTGCGTTAACGGTACAGCGCTCGCTTCCCGATCTGGCATTGCCAGCGCAAAATTGC‑CCCCCCATGCAGATCAATGCTCGAGCCTGCCCCGTACAAATTGCTTGCATGCTGGCAATTgcagc                                                          >  1:613883/1‑140 (MQ=255)                                                                       tACCGCCAAATCTAATTGCGTTAACGGTACAGCGCTCGCTTCCCGATCTGGCATTGCCAGCGCAAAATTGC‑CCCCCCATGCAGATCAATGCTCGAGCCTGCCCCGTACATATTGCTTGCATGCTGGCAATTGCAGCATGt                                                      >  2:838403/1‑140 (MQ=255)                                                                        aCCGCCAAATCTAATTGCGTTAACGGTACAGCGCTCGCTTCCCGATCTGGCATTGCCAGCGCAAAATTGC‑CCCCCCATGCAGATCAATGCTCGAGCCTGCCCCGTACATATTGCTTGCATGCTGGCAATTGCAGCATGTc                                                     <  2:330093/140‑1 (MQ=255)                                                                          cGCCAAATCTAATTGCGTTAACGGTACAGCGCTCGCTTCCCGATCTGGCATTGCCAGCGCAAAATTGC‑CCCCCCATGCAGATCAATGCTCGAGCCTGCCCCGTACATATTGCTTGCATGCTGGCAATTGCAGCATGTCCa                                                   <  1:1036081/140‑1 (MQ=255)                                                                           gCCAAATCTAATTGCGTTAACGGTACAGCGCTCGCTTCCCGATCTGGCATTGCCAGCGCAAAATTGC‑CCCCCCATGCAGATCAATGCTCGAGCCTGCCCCGTACATATTGCTTGCATGCTGGCAATTGCAGCATGTCCAg                                                  >  2:874807/1‑140 (MQ=255)                                                                           gCCAAATCTAATTGCGTTAACGGTACAGCGCTCGCTTCCCGATCTGGCATTGCCAGCGCAAAATTGC‑CCCCCCATGCAGATCAATGCTCGAGCCTGCCCCGTACATATTGCTTGCATGCTGGCAATTGCAGCATGTCCAg                                                  <  2:764762/140‑1 (MQ=255)                                                                           gCCAAATCTAATTGCGTTAACGGTACAGCGCTCGCTTCCCGATCTGGCATTGCCAGCGCAAAATTGC‑CCCCCCATGCAGATCAATGCTCGAGCCTGCCCCGTACATATTGCTTGCATGCTGGCAATTGCAGCATGTCCAg                                                  <  1:435662/140‑1 (MQ=255)                                                                            ccAAATCTAATTGCGTTAACGGTACAGCGCTCGCTTCCCGATCTGGCATTGCCAGCGCAAAATTGC‑CCCCCCATGCAGATCAATGCTCGAGCCTGCCCCGTACATATTGCTTGCATGCTGGCAATTGCAGCATGTCCAgg                                                 >  2:698733/1‑140 (MQ=255)                                                                               aaTCTAATTGCGTTAACGGTACAGCGCTCGCTTCCCGATCTGGCATTGCCAGCGCAAAATTGC‑CCCCCCATGCAGATCAATGCTCGAGCCTGCCCCGTACATATTGCTTGCATGCTGGCAATTGCAGCATGTCCAGGTGc                                              <  1:838403/140‑1 (MQ=255)                                                                                  cTAATTGCGTTAACGGTACAGCGCTCGCTTCCCGATCTGGCATTGCCAGCGCAAAATTGC‑CCCCCCATGCAGATCAATGCTCGAGCCTGCCCCGTACATATTGCTTGCATGCTGGCAATTGCAGCATGTCCAGGTGCATg                                           <  2:178197/140‑1 (MQ=255)                                                                                    aaTTGCGTTAACGGTACAGCGCTCGCTTCCCGATCTGGCATTGCCAGCGCAAAATTGC‑CCCCCCATGCAGATCAATGCTCGAGCCTGCCCCGTACATATTGCTTGCATGCTGGCAATTGCAGCATGTCCAGGTGCATGAg                                         <  1:944477/140‑1 (MQ=255)                                                                                     aTTGCGTTAACGGTACAGCGCTCGCTTCCCGATCTGGCATTGCCAGCGCAAAATTGC‑CCCCCCATGCAGATCAATGCTCGAGCCTGCCCCGTACATATTGCTTGCATGCTGGCAATTGCAGCATGTCCAGGTGCATGAgg                                        >  2:716283/1‑140 (MQ=255)                                                                                      ttGCGTTAACGGTACAGCGCTCGCTTCCCGATCTGGCATTGCCAGCGCAAAATTGC‑CCCCCCATGCAGATCAATGCTCGAGCCTGCCCCGTACATATTGCTTGCATGCTGGCAATTGCAGCATGTCCAGGTGCATGAGGt                                       <  2:1080049/140‑1 (MQ=255)                                                                                      ttGCGTTAACGGTACAGCGCTCGCTTCCCGATCTGGCATTGCCAGCGCAAAATTGC‑CCCCCCATGCAGATCAATGCTCGAGCCTGCCCCGTACATATTGCTTGCATGCTGGCAATTGCAGCATGTCCAGGTGCATGAGGt                                       <  2:196021/140‑1 (MQ=255)                                                                                      ttGCGTTAACGGTACAGCGCTCGCTTCCCGATCTGGCATTGCCAGCGCAAAATTGC‑CCCCCCATGCAGATCAATGCTCGAGCCTGCCCCGTACATATTGCTTGCATGCTGGCAATTGCAGCATGTCCAGGTGCATGAGGt                                       <  2:340485/140‑1 (MQ=255)                                                                                         cGTTAACGGTACAGCGCTCGCTTCCCGATCTGGCATTGCCAGCGCAAAATTGC‑CCCCCCATGCAGATCAATGCTCGAGCCTGCCCCGTACATATTGCTTGCATGCTGGCAATTGCAGCATGTCCAGGTGCATGAGGTggg                                    >  1:55766/1‑140 (MQ=255)                                                                                          gTTAACGGTACAGCGCTCGCTTCCCGATCTGGCATTGCCAGCGCAAAATTGC‑CCCCCCATGCAGATCAATGCTCGAGCCTGCCCCGTACATATTGCTTGCATGCTGGCAATTGCAGCATGTCCAGGTGCATGAGGTgggg                                   <  1:837235/140‑1 (MQ=255)                                                                                                   aCAGCGCTCGCTTCCCGATCTGGCATTGCCAGCGCAAAATTGC‑CCCCCCATGCAGATCAATGCTCGAGCCTGCCCCGTACATATTGCTTGCATGCTGGCAATTGCAGCATGTCCAGGTGCATGAGGTGGGGTGAAGCCAt                          <  1:457553/140‑1 (MQ=255)                                                                                                    cAGCGCTCGCTTCCCGATCTGGCATTGCCAGCGCAAAATTGC‑CCCCCCATGCAGATCAATGCTCGAGCCTGCCCCGTACATATTGCTTGCATGCTGGCAATTGCAGCATGTCCAGGTGCATGAGGTGGGGTGAAGCCATa                         >  2:283839/1‑140 (MQ=255)                                                                                                       cgctcgctTCCCGATCTGGCATTGCCAGCGCAAAATTGC‑CCCCCCATGCAGATCAATGCTCGAGCCTGCCCCGTACATATTGCTTGCATGCTGGCAATTGCAGCATGTCCAGGTGCATGAGGTGGGGTGAAGCCATAgcg                      <  1:1060209/140‑1 (MQ=255)                                                                                                        gctcgctTCCCGATCTGGCATTGCCAGCGCAAAATTGC‑CCCCCCATGCAGATCAATGCTCGAGCCTGCCCCGTACATATTGCTTGCATGCTGGCAATTGCAGCATGTCCAGGTGCATGAGGTGGGGTGAAGCCATAgcgc                     <  1:283839/140‑1 (MQ=255)                                                                                                        gctcgctTCCCGATCTGGCATTGCCAGCGCAAAATTGC‑CCCCCCATGCAGATCAATGCTCGAGCCTGCCCCGTACATATTGCTTGCATGCTGGCAATTGCAGCATGTCCAGGTGCAAGAGGTGGGGTGAAGCCATAgcgc                     >  2:398158/1‑140 (MQ=255)                                                                                                         ctcgctTCCCGATCTGGCATTGCCAGCGCAAAATTGCCCCCCCCATGCAGATCAATGCTCGAGCCTGCCCCGTACATATTGCTTGCATGCTGGCAATTGCAGCATGTCCAGGTGCATGAGGTGGGGTGAAGCCATAgcgc                     <  1:410351/140‑1 (MQ=255)                                                                                                            gctTCCCGATCTGGCATTGCCAGCGCAAAATTGC‑CCCCCCATGCAGATCAATGCTCGAGCCTGCCCCGTACATATTGCTTGCATGCTGGCAATTGCAGCATGTCCAGGTGCATGAGGTGGGGTGAAGCCATAGCGCTCAc                 <  1:698733/140‑1 (MQ=255)                                                                                                               tCCCGATCTGGCATTGCCAGCGCAAAATTGC‑CCCCCCATGCAGATCAATGCTCGAGCCTGCCCCGTACATATTGCTTGCATGCTGGCAATTGCAGCATGTCCAGGTGCATGAGGTGGGGTGAAGCCATAGCGCTCACCCa              >  1:657655/1‑140 (MQ=255)                                                                                                                  cGATCTGGCATTGCCAGCGCAAAATTGC‑CCCCCCATGCAGATCAATGCTCGAGCCTGCCCCGTACATATTGCTTGCATGCTGGCAATTGCAGCATGTCCAGGTGCATGAGGTGGGGTGAAGCCATAGCGCTCACCCAGAc           <  1:716283/140‑1 (MQ=255)                                                                                                                       tGGCATTGCCAGCGCAAAATTGC‑CCCCCCATGCAGATCAATGCTCGAGCCTGCCCCGTACATATTGCTTGCATGCTGGCAATTGCAGCATGTCCAGGTGCATGAGGTGGGGTGAAGCCATAGCGCTCACCCAGACGAGcc      <  2:657655/140‑1 (MQ=255)                                                                                                                           aTTGCCAGCGCAAAATTGC‑CCCCCCATGCAGATCAATGCTCGAGCCTGCCCCGTACATATTGCTTGCATGCTGGCAATTGCAGCATGTCCAGGTGCATGAGGTGGGGTGAAGCCATAGCGCTCACCCAGACGAGCCAGaa  <  2:937599/140‑1 (MQ=255)                                                                                                                                             |                                                                                                                           CCAGGACCGGCAGAATATAGCTATGCCGTGCGGTCAACAGATGAGAGCGGTTAAGCTTAGTGGCTACGTGTACCGCCAAATCTAATTGCGTTAACGGTACAGCGCTCGCTTCCCGATCTGGCATTGCCAGCGCAAAATTG‑‑CCCCCCATGCAGATCAATGCTCGAGCCTGCCCCGTACATATTGCTTGCATGCTGGCAATTGCAGCATGTCCAGGTGCATGAGGTGGGGTGAAGCCATAGCGCTCACCCAGACGAGCCAGAA  >  NC_000913/1584956‑1585216

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑