Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,240,079	T→C	L46L (TTA→CTA)	sstT →	serine/threonine:Na(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,240,079	0	T	C	100.0%	164.9 / NA	46	L46L (TTA→CTA)	sstT	serine/threonine:Na(+) symporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/25);  total (21/25)

AATGACTACGCAACGTTCACCGGGGCTATTCCGGCGTCTGGCTCATGGCAGCCTGGTAAAACAAATCCTGGTCGGCCTTGTTCTGGGGATTCTTCTGGCATGGATCTCAAAACCCGCGGCGGAAGCTGTTGGTCTGTTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCTATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCT  >  NC_000913/3239943‑3240203                                                                                                                                         |                                                                                                                             aaTGACTACGCAACGTTCACCGGGGCTATTCCGGCGTCTGGCTCATGGCAGCCTGGTAAAACAAATCCTGGTCGGCCTTGTTCTGGGGATTCTTCTGGCATGGATCTCAAAACCCGCGGCGGAAGCTGTTGGTCTGCTAg                                                                                                                           <  2:628400/140‑1 (MQ=255) aaTGACTACGCAACGTTCACCGGGGCTATTCCGGCGTCTGGCTCATGGCAGCCTGGTAAAACAAATCCTGGTCGGCCTTGTTCTGGGGATTCTTCTGGCATGGATCTCAAAACCCGCGGCGGAAGCTGTTGGTCTGCTAg                                                                                                                           <  1:920950/140‑1 (MQ=255)    gACTACGCAACGTTCACCGGGGCTATTCCGGCGTCTGGCTCATGGCAGCCTGGTAAAACAAATCCTGGTCGGCCTTGTTCTGGGGATTCTTCTGGCATGGATCTCAAAACCCGCGGCGGAAGCTGTTGGTCTGCTAGGTa                                                                                                                        <  1:399952/140‑1 (MQ=255)         cGCAACGTTCACCGGGGCTATTCCGGCGTCTGGCTCATGGCAGCCTGGTAAAACAAATCCTGGTCGGCCTTGTTCTGGGGATTCTTCTGGCATGGATCTCAAAACCCGCGGCGGAAGCTGTTGGTCTGCTAGGTACTTTg                                                                                                                   >  1:570138/1‑140 (MQ=255)                   aCCGGGGCTATTCCGGCGTCTGGCTCATGGCAGCCTGGTAAAACAAATCCTGGTCGGCCTTGTTCTGGGGATTCTTCTGGCATGGATCTCAAAACCCGCGGCGGAAGCTGTTGGTCTGCTAGGTACTTTGTTCGTCGgcg                                                                                                         <  2:552553/140‑1 (MQ=255)                        ggCTATTCCGGCGTCTGGCTCATGGCAGCCTGGTAAAACAAATCCTGGTCGGCCTTGTTCTGGGGATTCTTCTGGCATGGATCTCAAAACCCGCGGCGGAAGCTGTTGGTCTGCTAGGTACTTTGTTCGTCGGCGCACTg                                                                                                    >  1:426182/1‑140 (MQ=255)                          cTATTCCGGCGTCTGGCTCATGGCAGCCTGGTAAAACAAATCCTGGTCGGCCTTGTTCTGGGGATTCTTCTGGCATGGATCTCAAAACCCGCGGCGGAAGCTGTTGGTCTGCTAGGTACTTTGTTCGTCGGCGCACTGaa                                                                                                  >  1:508171/1‑140 (MQ=255)                                      cTGGCTCATGGCAGCCTGGTAAAACAAATCCTGGTCGGCCTTGTTCTGGGGATTCTTCTGGCATGGATCTCAAAACCCGCGGCGGAAGCTGTTGGTCTGCTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGcccc                                                                                      <  2:70354/140‑1 (MQ=255)                                      cTGGCTCATGGCAGCCTGGTAAAACAAATCCTGGTCGGCCTTGTTCTGGGGATTCTTCTGGCATGGATCTCAAAACCCGCGGCGGAAGCTGTTGGTCTGCTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGcccc                                                                                      <  2:291813/140‑1 (MQ=255)                                       tGGCTCATGGCAGCCTGGTAAAACAAATCCTGGTCGGCCTTGTTCTGGGGATTCTTCTGGCATGGATCTCAAAACCCGCGGCGGAAGCTGTTGGTCTGCTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGccccc                                                                                     >  1:748470/1‑140 (MQ=255)                                       tGGCTCATGGCAGCCTGGTAAAACAAATCCTGGTCGGCCTTGTTCTGGGGATTCTTCTGGCATGGATCTCAAAACCCGCGGCGGAAGCTGTTGGTCTGCTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTAccccc                                                                                     >  1:491473/1‑140 (MQ=255)                                                    ccTGGTAAAACAAATCCTGGTCGGCCTTGTTCTGGGGATTCTTCTGGCATGGATCTCAAAACCCGCGGCGGAAGCTGTTGGTCTGCTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGa                                                                        >  2:100897/1‑140 (MQ=255)                                                    ccTGGTAAAACAAATCCTGGTCGGCCTTGTTCTGGGGATTCTTCTGGCATGGATCTCAAAACCCGCGGCGGAAGCTGTTGGTCTGCTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGa                                                                        <  2:825533/140‑1 (MQ=255)                                                    ccTGGTAAAACAAATCCTGGTCGGCCTTGTTCTGGGGATTCTTCTGGCATGGATCTCAAAACCCGCGGCGGAAGCTGTTGGTCTGCTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGa                                                                        >  1:494566/1‑140 (MQ=255)                                                        gTAAAACAAATCCTGGTCGGCCTTGTTCTGGGGATTCTTCTGGCATGGATCTCAAAACCCGCGGCGGAAGCTGTTGGTCTGCTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCt                                                                    >  1:793095/1‑140 (MQ=255)                                                             aCAAATCCTGGTCGGCCTTGTTCTGGGGATTCTTCTGGCATGGATCTCAAAACCCGCGGCGGAAGCTGTTGGTCTGCTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGa                                                               <  2:1059647/140‑1 (MQ=255)                                                                aaTCCTGGTCGGCCTTGTTCTGGGGATTCTTCTGGCATGGATCTCAAAACCCGCGGCGGAAGCTGTTGGTCTGCTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATgg                                                            <  2:508171/140‑1 (MQ=255)                                                                 aTCCTGGTCAGCCTTGTTCTGGGGATTCTTCTGGCATGGATCTCAAAACCCGCGGCGGAAGCTGTTGGTCTGCTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGc                                                           <  2:801395/140‑1 (MQ=255)                                                                   ccTGGTCGGCCTTGTTCTGGGGATTCTTCTGGCATGGATCTCAAAACCCGCGGCGGAAGCTGTTGGTCTGCTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCAt                                                         <  2:748470/140‑1 (MQ=255)                                                                    cTGGTCGGCCTTGTTCTGGGGATTCTTCTGGCATGGATCTCAAAACCCGCGGCGGAAGCTGTTGGTCTGCTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATc                                                        <  2:303081/140‑1 (MQ=255)                                                                       gTCGGCCTTGTTCTGGGGATTCTTCTGGCATGGATCTCAAAACCCGCGGCGGAAGCTGTTGGTCTGCTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCTAt                                                     >  1:233856/1‑140 (MQ=255)                                                                       gTCGGCCTTGTTCTGGGGATTCTTCTGGCATGGATCTCAAAACCCGCGGCGGAAGCTGTTGGTCTGCTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCTAt                                                     >  1:1008802/1‑140 (MQ=255)                                                                           gCCTTGTTCTGGGGATTCTTCTGGCATGGATCTCAAAACCCGCGGCGGAAGCTGTTGGTCTGCTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCTATTGCt                                                 <  2:793095/140‑1 (MQ=255)                                                                           gCCTTGTTCTGGGGATTCTTCTGGCATGGATCTCAAAACCCGCGGCGGAAGCTGTTGGTCTGCTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCTATTGCt                                                 >  1:325539/1‑140 (MQ=255)                                                                           gCCTTGTTCTGGGGATTCTTCTGGCATGGATCTCAAAACCCGCGGCGGAAGCTGTTGGTCTGCTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCTATTGCt                                                 <  2:570138/140‑1 (MQ=255)                                                                            ccTTGTTCTGGGGATTCTTCTGGCATGGATCTCAAAACCCGCGGCGGAAGCTGTTGGTCTGCTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGGTGCTGGTGATGGCATCTATTGCTa                                                <  2:1118245/140‑1 (MQ=255)                                                                            ccTTGTTCTGGGGATTCTTCTGGCATGGATCTCAAAACCCGCGGCGGAAGCTGTTGGTCTGCTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCTATTGCTa                                                <  2:491473/140‑1 (MQ=255)                                                                                      gggATTCTTCTGGCATGGATCTCAAAACCCGCGGCGGAAGCTGTTGGTCTGCTAGGGACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCTATTGCTAACCACCAGCa                                      >  2:266138/1‑140 (MQ=255)                                                                                                  gCATGGATCTCAAAACCCGCGGCGGAAGCTGTTGGTCTGCTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCTATTGCTAACCACCAGCACGGGCAGAAAAc                          >  1:220355/1‑140 (MQ=255)                                                                                                  gCATGGATCTCAAAACCCGCGGCGGAAGCTGTTGGTCTGCTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCTATTGCTAACCACCAGCACGGGCAGAAAAc                          >  2:829446/1‑140 (MQ=255)                                                                                                       gATCTCAAAACCCGCGGCGGAAGCTGTTGGTCTGCTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCTATTGCTAACCACCAGCACGGGCAGAAAACCAata                     >  1:442312/1‑140 (MQ=255)                                                                                                       gATCTCAAAACCCGCGGCGGAAGCTGTTGGTCTGCTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCTATTGCTAACCACCAGCACGGGCAGAAAACCAata                     >  2:699319/1‑140 (MQ=255)                                                                                                        aTCTCAAAACCCGCGGCGGAAGCTGTTGGTCTGCTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCTATTGCTAACCACCAGCACGGGCAGa                              <  1:115712/130‑1 (MQ=255)                                                                                                        aTCTCAAAACCCGCGGCGGAAGCTGTTGGTCTGCTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCTATTGCTAACCACCAGCACGGGCAGa                              >  2:115712/1‑130 (MQ=255)                                                                                                        aTCTCAAAACCCGCGGCGGAAGCTGTTGGTCTGCTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCTATTGCTAACCACCAGCACGGGCAGAAAACCAatat                    <  2:675389/140‑1 (MQ=255)                                                                                                           tcAAAACCCGCGGCGGAAGCTGTTGGTCTGCTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCTATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCg                 <  2:912146/140‑1 (MQ=255)                                                                                                                   cgcgGCGGAAGCTGTTGGTCTGCTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCTATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTAttt         >  1:269402/1‑140 (MQ=255)                                                                                                                    gcggcgGAAGCTGTTGGTCTGCTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCTATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCg                 >  1:884214/1‑131 (MQ=255)                                                                                                                    gcggcgGAAGCTGTTGGTCTGCTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCTATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCg                 <  2:884214/131‑1 (MQ=255)                                                                                                                     cggcggAAGCTGTTGGTCTGCTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCTATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTg       <  1:829446/140‑1 (MQ=255)                                                                                                                     cggcggAAGCTGTTGGTCTGCTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCTATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTg       >  1:455728/1‑140 (MQ=255)                                                                                                                     cggcggAAGCTGTTGGTCTGCTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCTATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTg       >  1:155628/1‑140 (MQ=255)                                                                                                                       gcggAAGCTGTTGGTCTGCTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCTATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGtt     <  2:494566/140‑1 (MQ=255)                                                                                                                       gcggAAGCTGTTGGTCTGCTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCTATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGtt     <  2:344389/140‑1 (MQ=255)                                                                                                                       gcggAAGCTGTTGGTCTGCTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCTATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGtt     <  2:155628/140‑1 (MQ=255)                                                                                                                       gcggAAGCTGTTGGTCTGCTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCTATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGtt     <  1:503994/140‑1 (MQ=255)                                                                                                                          gAAGCTGTTGGTCTGCTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCTATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCct  <  2:325539/140‑1 (MQ=255)                                                                                                                          gAAGCTGTTGGTCTGCTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCTATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCct  <  2:69451/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                             AATGACTACGCAACGTTCACCGGGGCTATTCCGGCGTCTGGCTCATGGCAGCCTGGTAAAACAAATCCTGGTCGGCCTTGTTCTGGGGATTCTTCTGGCATGGATCTCAAAACCCGCGGCGGAAGCTGTTGGTCTGTTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCTATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCT  >  NC_000913/3239943‑3240203

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑