Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,246,199	T→C	Y326H (TAT→CAT)	exuT →	hexuronate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,246,199	0	T	C	100.0%	233.3 / NA	64	Y326H (TAT→CAT)	exuT	hexuronate transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (40/24);  total (40/24)

GGTGGTTACCTGCCGCCGCTGTTCCAGCGTTGGTTTGGTGTGAACCTGATCGTTTCCCGTAAGATGGTCGTAACGCTGGGTGCAGTGCTGATGATTGGCCCGGGTATGATCGGCCTGTTCACCAACCCGTATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGGGATGTCCGCAT  >  NC_000913/3246070‑3246334                                                                                                                                  |                                                                                                                                        ggtggtTACCTGCCGCCGCTGTTCCAGCGTTGGTTTGGTGTGAACCTGATCGTTTCCCGTAAGATGGTCGTAACGCTGGGTGCAGTGCTTATGATTGGCCCGGGTATGATCGGCCTGTTCACCAACCCGCATGTCGCAAt                                                                                                                               >  1:294991/1‑140 (MQ=255)     gtTACCTGCCGCCGCTGTTCCAGCGTTGGTTTGGTGTGAACCTGATCGTTTCCCGTAAGATGGTCGTAACGCTGGGTGCAGTGCTGATGATTGGCCCGGGTATGATCGGCCTGTTCACCAACCCGCATGTCGCAATTAtg                                                                                                                           <  2:189366/140‑1 (MQ=255)     gtTACCTGCCGCCGCTGTTCCAGCGTTGGTTTGGTGTGAACCTGATCGTTTCCCGTAAGATGGTCGTAACGCTGGGTGCAGTGCTGATGATTGGCCCGGGTATGATCGGCCTGTTCACCAACCCGCATGTCGCAATTAtg                                                                                                                           <  1:607230/140‑1 (MQ=255)        aCCTGCCGCCGCTGTTCCAGCGTTGGTTTGGTGTGAACCTGATCGTTTCCCGTAAGATGGTCGTAACGCTGGGTGCAGTGCTGATGATTGGCCCGGGTATGATCGGCCTGTTCACCAACCCGCATGTCGCAATTAtgctg                                                                                                                        <  1:1021062/140‑1 (MQ=255)        aCCTGCCGCCGCTGTTCCAGCGTTGGTTTGGTGTGAACCTGATCGTTTCCCGTAAGATGGTCGTAACGCTGGGTGCAGTGCTGATGATTGGCCCGGGTATGATCGGCCTGTTCACCAACCCGCATGTCGCAATTAtgctg                                                                                                                        <  1:115926/140‑1 (MQ=255)        aCCTGCCACCGCTGTTCCAGCGTTGGTTTGGTGTGAACCTGATCGTTTCCCGTAAGATGGTCGTAACGCTGGGTGCAGTGCTGATGATTGGCCCGGGTATGATCGGCCTGTTCACCAACCCGCATGTCGCAATTAtg                                                                                                                           <  1:552383/137‑1 (MQ=255)        aCCTGCCACCGCTGTTCCAGCGTTGGTTTGGTGTGAACCTGATCGTTTCCCGTAAGATGGTCGTAACGCTGGGTGCAGTGCTGATGATTGGCCCGGGTATGATCGGCCTGTTCACCAACCCGCATGTCGCAATTAtg                                                                                                                           >  2:552383/1‑137 (MQ=255)             ccgccgCTGTTCCAGCGTTGGTTTGGTGTGAACCTGATCGTTTCCCGTAAGATGGTCGTAACGCTGGGTGCAGTGCTGATGATTGGCCCGGGTATGATCGGCCTGTTCACCAACCCGCATGTCGCAATTATGCTGCtgtg                                                                                                                   >  1:1040807/1‑140 (MQ=255)             ccgccgCTGTTCCAGCGTTGGTTTGGTGTGAACCTGATCGTTTCCCGTAAGATGGTCGTAACGCTGGGTGCAGTGCTGATGATTGGCCCGGGTATGATCGGCCTGTTCACCAACCCGCATGTCGCAATTATGCTGCtgtg                                                                                                                   >  2:79347/1‑140 (MQ=255)                ccgcTGTTCCAGCGTTGGTTTGGTGTGAACCTGATCGTTTCCCGTAAGATGGTCGTAACGCTGGGTGCAGTGCTGATGATTGGCCCGGGTATGATCGGCCTGTTCACCAACCCGCATGTCGCAATTATGCTGCTGTGTAt                                                                                                                >  1:683077/1‑140 (MQ=255)                  gcTGTTCCAGCGTTGGTTTGGTGTGAACCTGATCGTTTCCCGTAAGATGGTCGTAACGCTGGGTGCAGTGCTGATGATTGGCCCGGGTATGATCGGCCTGTTCACCAACCCGCATGTCGCAATTATGCTGCTGTGTATCg                                                                                                              <  2:706409/140‑1 (MQ=255)                            cGTTGGTTTGGTGTGAACCTGATCGTTTCCCGTAAGATGGTCGTAACGCTGGGTGCAGTGCTGATGATTGGCCCGGGTATGATCGGCCTGTTCACCAACCCGCATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGc                                                                                                    <  1:625736/140‑1 (MQ=255)                              ttggtttggtGTGAACCTGATCGTTTCCCGTAAGATGGTCGTAACGCTGGGTGCAGTGCTGATGATTGGCCCGGGTATGATCGGCCTGTTCACCAACCCGCATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGccc                                                                                                  >  1:41984/1‑140 (MQ=255)                              ttggtttggtGTGAACCTGATCGTTTCCCGTAAGATGGTCGTAACGCTGGGTGCAGTGCTGATGATTGGCCCGGGTATGATCGGCCTGTTCACCAACCCGCATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGccc                                                                                                  >  2:450100/1‑140 (MQ=255)                                ggtttggtGTGAACCTGATCGTTTCCCGTAAGATGGTCGTAACGCTGGGTGCAGTGCTGATGATTGGCCCGGGTATGATCGGCCTGTTCACCAACCCGCATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCccac                                                                                                <  1:87481/140‑1 (MQ=255)                                ggtttggtGTGAACCTGATCGTTTCCCGTAAGATGGTCGTAACGCTGGGTGCAGTGCTGATGATTGGCCCGGGTATGATCGGCCTGTTCACCAACCCGCATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCccac                                                                                                >  1:625764/1‑140 (MQ=255)                                    tggtGTGAACCTGATCGTTTCCCGTAAGATGGTCGTAACGCTGGGTGCAGTGCTGATGATTGGCCCGGGTATGATCGGCCTGTTCACCAACCCGCATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAgg                                                                                            <  2:333431/140‑1 (MQ=255)                                         tgAACCTGATCGTTTCCCGTAAGATGGTCGTAACGCTGGGTGCAGTGCTGATGATTGGCCCGGGTATGATCGGCCTGTTCACCAACCCGCATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCACTg                                                                                       >  2:586369/1‑140 (MQ=255)                                           aaCCTGATCGTTTCCCGTAAGATGGTCGTAACGCTGGGTGCAGTGCTGATGATTGGCCCGGGTATGATCGGCCTGTTCACCAACCCGCATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCACTGTc                                                                                     >  2:1045487/1‑140 (MQ=255)                                           aaCCTGATCGTTTCCCGTAAGATGGTCGTAACGCTGGGTGCAGTGCTGATGATTGGCCCGGGTATGAACGGCCTGTTCACCAACCTGCATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCACTGTc                                                                                     >  1:657748/1‑140 (MQ=255)                                            aCCTGATCGTTTCCCGTAAGATGGTCGTAACGCTGGGTGCAGTGCTGATGATTGGCCCGGGTATGATCGGCCTGTTCACCAACCCGCATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCACTGTCt                                                                                    <  2:560763/140‑1 (MQ=255)                                                 aTCGTTTCCCGTAAGATGGTCGTAACGCTGGGTGCAGTGCTGATGATTGGCCCGGGTATGATCGGCCTGTTCACCAACCCGCATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCccac                                                                                                <  1:567259/123‑1 (MQ=255)                                                 aTCGTTTCCCGTAAGATGGTCGTAACGCTGGGTGCAGTGCTGATGATTGGCCCGGGTATGATCGGCCTGTTCACCAACCCGCATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCccac                                                                                                >  2:567259/1‑123 (MQ=255)                                                 aTCGTTTCCCGTAAGATGGTCGTAACGCTGGGTGCAGTGCTGATGATTGGCCCGGGTATGATCGGCCTGTTCACCAACCCGCATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCACTGTCTGgcgc                                                                               >  1:452861/1‑140 (MQ=255)                                                 aTCGTTTCCCGTAAGATGGTCGTAACGCTGGGTGCAGTGCTGATGATTGGCCCGGGTATGATCGGCCTGTTCACCAACCCGCATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCACTGTCTGgcgc                                                                               >  2:1069249/1‑140 (MQ=255)                                                        cccGTAAGATGGTCGTAACGCTGGGTGCAGTGCTGATGATTGGCCCGGGTATGATCGGCCTGTTCACCAACCCGCATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGAtt                                                                        <  2:683077/140‑1 (MQ=255)                                                           gTAAGATGGGCCTGACGCTGGGTGCAGGGCTGATGATTGGCCTGGGTATGGTTGGCCTGTTCACCACCCCGCATCTTGCAATTATGCTGCTGTATATCGGTGGTTTTGACCACCAGGCACTGTCTGGCGCGCTGATTACg                                                                     <  2:657748/140‑1 (MQ=255)                                                             aaGATGGTCGTAACGCTGGGTGCAGTGCTTATGATTGGCCCGGGTATGATCGGCCTGTTCACCAACCCGCATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGct                                                                   <  2:294991/140‑1 (MQ=255)                                                             aaGATGGTCGTAACGCTGGGTGCAGTGCTGATGATTGGCCCGGGTATGATCGGCCTGTTCACCAACCCGCATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGct                                                                   <  1:1069249/140‑1 (MQ=255)                                                             aaGATGGTCGTAACGCTGGGTGCAGTGCTGATGATTGGCCCGGGTATGATCGGCCTGTTCACCAACCCGCATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGct                                                                   <  2:1040807/140‑1 (MQ=255)                                                                aTGGTCGTAACGCTGGGTGCAGTGCTGATGATTGGCCCGGGTATGATCGGCCTGTTCACCAACCCGCATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGctctc                                                                >  2:682889/1‑140 (MQ=255)                                                                aTGGTCGTAACGCTGGGTGCAGTGCTGATGATTGGCCCGGGTATGATCGGCCTGTTCACCAACCCGCATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGctctc                                                                >  2:78010/1‑140 (MQ=255)                                                                aTGGTCGTAACGCTGGGTGCAGTGCTGATGATTGGCCCGGGTATGATCGGCCTGTTCACCAACCCGCATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGct                                                                   >  1:702557/1‑137 (MQ=255)                                                                aTGGTCGTAACGCTGGGTGCAGTGCTGATGATTGGCCCGGGTATGATCGGCCTGTTCACCAACCCGCATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGct                                                                   <  2:702557/137‑1 (MQ=255)                                                                aTGGTCGTAACGCTGGGTGCAGTGCTGATGA‑TGGCCCGGGTATGATCGGCCTGTTCACCAACCCGCATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGctctct                                                               >  2:991620/1‑140 (MQ=255)                                                                     cGTAACGCTGGGTGCAGTGCTGATGATTGGCCCGGGTATGATCGGCCTGTTCACCAACCCGCATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCg                                                           <  1:79347/140‑1 (MQ=255)                                                                       tAACGCTGGGTGCAGTGCTGATGATTGGCCCGGGTATGATCGGCCTGTTCACCAACCCGCATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGAt                                                         <  1:79070/140‑1 (MQ=255)                                                                       tAACGCTGGGTGCAGTGCTGATGATTGGCCCGGGTATGATCGGCCTGTTCACCAACCCGCATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGAt                                                         <  2:579972/140‑1 (MQ=255)                                                                              gggTGCAGTGCTGATGATTGGCCCGGGTATGATCGGCCTGTTCACCAACCCGCATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTg                                                  >  1:88383/1‑140 (MQ=255)                                                                              gggTGCAGTGCTGATGATTGGCCCGGGTATGATCGGCCTGTTCACCAACCCGCATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTg                                                  >  2:464609/1‑140 (MQ=255)                                                                               ggTGCAGTGCTGATGATTGGCCCGGGTATGATCGGCCTGTTCACCAACCCGCATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGct                                                                   <  1:330782/122‑1 (MQ=255)                                                                               ggTGCAGTGCTGATGATTGGCCCGGGTATGATCGGCCTGTTCACCAACCCGCATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGct                                                                   >  2:330782/1‑122 (MQ=255)                                                                                    aGTGCTGATGATTGGCCCGGGTATGATCGGCCTGTTCACCAACCCGCATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTa                                            >  1:605279/1‑140 (MQ=255)                                                                                         tgatgaTTGGCCCGGGTATGATCGGCCTGTTCACCAACCCGCATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGaa                                       >  1:920167/1‑140 (MQ=255)                                                                                           atgatTGGCCCGGGTATGATCGGCCTGTTCACCAACCCGCATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGt                                     >  1:340570/1‑140 (MQ=255)                                                                                                tGGCCCGGGTATGATCGGCCTGTTCACCAACCCGCATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCaa                                >  1:549007/1‑140 (MQ=255)                                                                                                tGGCCCGGGTATGATCGGCCTGTTCACCAACCCGCATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCaa                                >  1:530131/1‑140 (MQ=255)                                                                                                tGGCCCGGGTATGATCGGCCTGTTCACCAACCCGCATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCaa                                >  1:369127/1‑140 (MQ=255)                                                                                                tGGCCCGGGTATGATCGGCCTGTTCACCAACCCGCATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCaa                                >  2:1018217/1‑140 (MQ=255)                                                                                                  gCCCGGGTATGATCGGCCTGTTCACCAACCCGCATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACg                              >  1:188316/1‑140 (MQ=255)                                                                                                  gCCCGGGTATGATCGGCCTGTTCACCAACCCGCATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCACTGTCTGGAGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTgg                                   <  1:123278/135‑1 (MQ=255)                                                                                                  gCCCGGGTATGATCGGCCTGTTCACCAACCCGCATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCACTGTCTGGAGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTgg                                   >  2:123278/1‑135 (MQ=255)                                                                                                      gggTATGATCGGCCTGTTCACCAACCCGCATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCaa                          <  1:1018217/140‑1 (MQ=255)                                                                                                       ggTATGATCGGCCTGTTCACCAACCCGCATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCaaa                         >  2:1092324/1‑140 (MQ=255)                                                                                                             aTCGGCCTGTTCACCAACCCGCATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGttt                   >  2:344923/1‑140 (MQ=255)                                                                                                             aTCGGCCTGTTCACCAACCCGCATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGttt                   >  2:324177/1‑140 (MQ=255)                                                                                                                 gCCTGTTCACCAACCCGCATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAAcc               >  2:548664/1‑140 (MQ=255)                                                                                                                 gCCTGTTCACCAACCCGCATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAAcc               >  2:486661/1‑140 (MQ=255)                                                                                                                 gCCTGTTCACCAACCCGCATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAAcc               >  2:328296/1‑140 (MQ=255)                                                                                                                  ccTGTTCACCAACCCGCATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCg              >  2:72243/1‑140 (MQ=255)                                                                                                                  ccTGTTCACCAACCCGCATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCg              >  2:14386/1‑140 (MQ=255)                                                                                                                          ccAACCCGCATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGGGATGTcc      >  1:220813/1‑140 (MQ=255)                                                                                                                           cAACCCGCATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGGGATGTCCg     <  2:188316/140‑1 (MQ=255)                                                                                                                             aCCCGCATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGGGATGTCCGCa   <  1:486661/140‑1 (MQ=255)                                                                                                                              cccGCATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGGGATGTCCGCAt  >  1:830983/1‑140 (MQ=255)                                                                                                                                  |                                                                                                                                        GGTGGTTACCTGCCGCCGCTGTTCCAGCGTTGGTTTGGTGTGAACCTGATCGTTTCCCGTAAGATGGTCGTAACGCTGGGTGCAGTGCTGATGATTGGCCCGGGTATGATCGGCCTGTTCACCAACCCGTATGTCGCAATTATGCTGCTGTGTATCGGTGGTTTTGCCCACCAGGCACTGTCTGGCGCGCTGATTACGCTCTCTTCCGATGTGTTTGGTCGTAACGAAGTGGCAACGGCAAACGGTTTAACCGGGATGTCCGCAT  >  NC_000913/3246070‑3246334

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑