Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,379,381 T→C N206S (AAC→AGC)  zapE ← cell division factor ZapE with ATPase activity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,379,3810TC96.4% 185.3 / ‑1.9 55N206S (AAC→AGC) zapEcell division factor ZapE with ATPase activity
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (24/29);  total (25/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.35e-01

TGAATTTTCTCGTTTCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACGTTCATTACATCACAATGCTGTTTAATGGCATCGATTGCAGGCAGAAAACGCGCACGTTGCAGGCCATTTCGATAAAGTTCATCCGGCGGAATATTTGACGTCGCTACCAGGGTAATACCGCGAGCGAACAGGGC  >  NC_000913/3379245‑3379514
                                                                                                                                        |                                                                                                                                     
tGAATTTTCTCGTTTCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACGCTCa                                                                                                                                    <  1:991337/140‑1 (MQ=255)
tGAATTTTCTCGTTTCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACGCTCa                                                                                                                                    <  2:67198/140‑1 (MQ=255)
  aaTTTTCTCGTTT‑CCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACGCTCATTa                                                                                                                                 <  1:1072562/140‑1 (MQ=255)
           gTTTCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACGCTCATTACATCACaa                                                                                                                         <  2:434186/140‑1 (MQ=255)
            tttCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACGCTCATTACATCACAAt                                                                                                                        <  1:1037554/140‑1 (MQ=255)
                                 ccATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACGCTCATTACATCACAATGCTGTTTAATGGCATCGATTg                                                                                                   <  2:248520/140‑1 (MQ=255)
                                 ccATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACCCAGACGATAATCAACACCAGCGTCCACGCTCATTACATCACAATGCTGTTTAATGGCATCGATTg                                                                                                   <  1:1011022/140‑1 (MQ=255)
                                       tttATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACGCTCATTACATCACAATGCTGTTTAATGGCATCGATTgcaggca                                                                                             >  1:177229/1‑140 (MQ=255)
                                       tttATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACGCTCATTACATCACAATGCTGTTTAATGGCATCGATTgcaggca                                                                                             >  1:272489/1‑140 (MQ=255)
                                          aTCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACGCTCATTACATCACAATGCTGTTTAATGGCATCGATTGCAGGCAGaa                                                                                          >  1:355381/1‑140 (MQ=255)
                                            ccATTTGCGCCCGGGTTTCATCGTGAAGTGGCTAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACGCTCATTACATCACAATGCTGTTTAATGGCATCGATTGCAGGCAGaaaa                                                                                        >  1:418055/1‑140 (MQ=255)
                                            ccATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACGCTCATTACATCACAATGCTGTTTAATGGCATCGATTGCAGGCAGaaaa                                                                                        >  1:1129568/1‑140 (MQ=255)
                                            ccATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACGCTCATTACATCACAATGCTGTTTAATGGCATCGATTGCAGGCAGaaaa                                                                                        >  1:115012/1‑140 (MQ=255)
                                               tttGCGCCCGGGTTTCATCGTGAAGTGGCTAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACGCTCATTACATCACAATGCTGTTTAATGGCATCGATTGCAGGCAGAAAAcgc                                                                                     <  2:418055/140‑1 (MQ=255)
                                                  gcgcCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACGCTCATTACATCACAATGCTGTTTAATGGCATCGATTGCAGGCAGAAAACGCGCa                                                                                  <  2:684168/140‑1 (MQ=255)
                                                  gcgcCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACGCTCATTACATCACAATGCTGTTTAATGGCATCGATTGCAGGCAGAAAACGCGCa                                                                                  <  1:57862/140‑1 (MQ=255)
                                                     cccGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACGTTCATTACATCACAATGCTGTTTAATGGCATCGATTGCAGGCAGAAAACGCGCa                                                                                  >  2:1076029/1‑137 (MQ=255)
                                                     cccGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACGTTCATTACATCACAATGCTGTTTAATGGCATCGATTGCAGGCAGAAAACGCGCa                                                                                  <  1:1076029/137‑1 (MQ=255)
                                                         ggTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACGCTCATTACATCACAATGCTGTTTAATGGCATCGATTGCAGGCAGAAAACGCGCACGTTGCa                                                                           >  2:396131/1‑140 (MQ=255)
                                                                tCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACGCTCATTACATCACAATGCTGTTTAATGGCATCGATTGCAGGCAGAAAACGCGCACGTTGCAGGCCAtt                                                                    <  2:177229/140‑1 (MQ=255)
                                                                  gTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACGCTCATTACATCACAATGCTGTTTAATGGCATCGATTGCAGGCAGAAAACGCGCACGTTGCAGGCCATTTc                                                                  >  2:215693/1‑140 (MQ=255)
                                                                    gAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACGCTCATTACATCACAATGCTGTTTAATGGCATCGATTGCAGGCAGAAAACGCGCACGTTGCAGGCCATTTCGa                                                                <  1:47089/140‑1 (MQ=255)
                                                                         ggCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACGCTCATTACATCACAATGCTGTTTAATGGCATCGATTGCAGGCAGAAAACGCGCACGTTGCAGGCCATTTCGATAAAg                                                           >  2:1144807/1‑140 (MQ=255)
                                                                                  cacaGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACGCTCATTACATCACAATGCTGTTTAATGGCATCGATTGCAGGCAGAAAACGCGCACGTTGCAGGCCATTTCGATAAAGTTCATCcgg                                                  <  2:355381/140‑1 (MQ=255)
                                                                                      gATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACGCTCATTACATCACAATGCTGTTTAATGGCATCGATTGCAGGCAGAAAACGCGCACGTTGCAGGCCATTTCGATAAAGTTCATCCGGCGGa                                              <  1:215693/140‑1 (MQ=255)
                                                                                         gcgcCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACGCTCATTACATCACAATGCTGTTTAATGGCATCGATTGCAGGCAGAAAACGCGCACGTTGCAGGCCATTTCGATAAAGTTCATCCGGCGGAata                                           >  2:1003484/1‑140 (MQ=255)
                                                                                            ccTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACGCTCATTACATCACAATGCTGTTTAATGGCATCGATTGCAGGCAGAAAACGCGCACGTTGCAGGCCATTTCGATAAAGTTCATCCGGCGGAATAttt                                        >  2:300420/1‑140 (MQ=255)
                                                                                                 gtgagtgTACGCAGACGATAATCAACACCAGCGTCCACGCTCATTACATCACAATGCTGTTTAATGGCATCGATTGCAGGCAGAAAACGCGCACGTTGCAGGCCATTTCGATAAAGTTCATCCGGCGGAATATTTGACGt                                   <  2:270843/140‑1 (MQ=255)
                                                                                                 gtgagtgTACGCAGACGATAATCAACACCAGCGTCCACGCTCATTACATCACAATGCTGTTTAATGGCATCGATTGCAGGCAGAAAACGCGCACGTTGCAGGCCATTTCGATAAAGTTCATCCGGCGGAATATTTGACGt                                   <  2:855416/140‑1 (MQ=255)
                                                                                                  tgagtgTACGCAGACGATAATCAACACCAGCGTCCACGCTCATTACATCACAATGCTGTTTAATGGCATCGATTGCAGGCAGAAAACGCGCACGTTGCAGGCCATTTCGATAAAGTTCATCCGGCGGAATATTTGACGTc                                  <  2:749022/140‑1 (MQ=255)
                                                                                                      tgtACGCAGACGATAATCAACACCAGCGTCCACGCTCATTACATCACAATGCTGTTTAATGGCATCGATTGCAGGCAGAAAACGCGCACGTTGCAGGCCATTTCGATAAAGTTCATCCGGCGGAATATTTGACGTCGCTa                              >  2:258075/1‑140 (MQ=255)
                                                                                                       gtACGCAGACGATAATCAACACCAGCGTCCACGCTCATTACATCACAATGCTGTTTAATGGCATCGATTGCAGGCAGAAAACGCGCACGTTGCAGGCCATTTCGATAAAGTTCATCCGGCGGAATATTTGACGTCGCTAc                             <  2:1099641/140‑1 (MQ=255)
                                                                                                       gtACGCAGACGATAATCAACACCAGCGTCCACGCTCATTACATCACAATGCTGTTTAATGGCATCGATTGCAGGCAGAAAACGCGCACGTTGCAGGCCATTTCGATAAAGTTCATCCGGCGGAATATTTGACGTCGCTAc                             <  1:258075/140‑1 (MQ=255)
                                                                                                       gtACGCAGACGATAATCAACACCAGCGTCCACGCTCATTACATCACAATGCTGTTTAATGGCATCGATTGCAGGCAGAAAACGCGCACGTTGCAGGCCATTTCGATAAAGTTCATCCGGCGGAATATTTGACGTCGCTAc                             <  1:396131/140‑1 (MQ=255)
                                                                                                              gACGATAATCAACACCAGCGTCCACGCTCATTACATCACAATGCTGTTTAATGGCATCGATTGCAGGCAGAAAACGCGCACGTTGCAGGCCATTTCGATAAAGTTCATCCGGCGGAATATTTGAc                                     <  1:632090/125‑1 (MQ=255)
                                                                                                              gACGATAATCAACACCAGCGTCCACGCTCATTACATCACAATGCTGTTTAATGGCATCGATTGCAGGCAGAAAACGCGCACGTTGCAGGCCATTTCGATAAAGTTCATCCGGCGGAATATTTGAc                                     >  2:632090/1‑125 (MQ=255)
                                                                                                               aCGATAATCAACACCAGCGTCCACGCTCATTACATCACAATGCTGTTTAATGGCATCGATTGCAGGCAGAAAACGCGCACGTTGCAGGCCATTTCGATAAAGTTCATCCGGCGGAATATTTGACGTCGc                                >  2:433031/1‑129 (MQ=255)
                                                                                                               aCGATAATCAACACCAGCGTCCACGCTCATTACATCACAATGCTGTTTAATGGCATCGATTGCAGGCAGAAAACGCGCACGTTGCAGGCCATTTCGATAAAGTTCATCCGGCGGAATATTTGACGTCGc                                <  1:433031/129‑1 (MQ=255)
                                                                                                               aCGATAATCAACACCAGCGTCCACGCTCATTACATCACAATGCTGTTTAATGGCATCGATTGCAGGCAGAAAACGCGCACGTTGCAGGCCATTTCGATAAAGTTCATCCGGCGGAATATTTGACGTCGCTACCAGGGTaa                     >  2:161743/1‑140 (MQ=255)
                                                                                                               aCGATAATCAACACCAGCGTCCACGCTCATTACATCACAATGCTGTTTAATGGCATCGATTGCAGGCAGAAAACGCGCACGTTGCAGGCCATTTCGATAAAGTTCATCCGGCGGAATATTTGACGTCGCTACCAGGGTaa                     >  1:1087562/1‑140 (MQ=255)
                                                                                                                cGATAATCAACACCAGCGTCCACGCTCATTACATCACAATGCTGTTTAATGGCATCGATTGCAGGCAGAAAACGCGCACGTTGCAGGCCATTTCGATAAAGTTCATCCGGCGGAATATTTGACGTCGCTAc                             >  1:985797/1‑131 (MQ=255)
                                                                                                                cGATAATCAACACCAGCGTCCACGCTCATTACATCACAATGCTGTTTAATGGCATCGATTGCAGGCAGAAAACGCGCACGTTGCAGGCCATTTCGATAAAGTTCATCCGGCGGAATATTTGACGTCGCTAc                             >  1:893948/1‑131 (MQ=255)
                                                                                                                cGATAATCAACACCAGCGTCCACGCTCATTACATCACAATGCTGTTTAATGGCATCGATTGCAGGCAGAAAACGCGCACGTTGCAGGCCATTTCGATAAAGTTCATCCGGCGGAATATTTGACGTCGCTAc                             <  2:893948/131‑1 (MQ=255)
                                                                                                                cGATAATCAACACCAGCGTCCACGCTCATTACATCACAATGCTGTTTAATGGCATCGATTGCAGGCAGAAAACGCGCACGTTGCAGGCCATTTCGATAAAGTTCATCCGGCGGAATATTTGACGTCGCTAc                             <  2:985797/131‑1 (MQ=255)
                                                                                                                cGATAATCAACACCAGCGTCCACGCTCATTACATCACAATGCTGTTTAATGGCATCGATTGCAGGCAGAAAACGCGCACGTTGCAGGCCATTTCGATAAAGTTCATCCGGCGGAATATTTGACGTCGCTACCAGGGTAAt                    >  1:104596/1‑140 (MQ=255)
                                                                                                                       cAACACCAGCGTCCACGCTCATTACATCACAATGCTGTTTAATGGCATCGATTGCAGGCAGAAAACGCGCACGTTGCAGGCCATTTCGATAAAGTTCATCCGGCGGAATATTTGACGTCGCTACCAGGGTAATACCgcga             <  2:104596/140‑1 (MQ=255)
                                                                                                                       cAACACCAGCGTCCACGCTCATTACATCACAATGCTGTTTAATGGCATCGATTGCAGGCAGAAAACGCGCACGTTGCAGGCCATTTCGATAAAGTTCATCCGGCGGAATATTTGACGTCGCTACCAGGGTAATACCgcga             <  2:253369/140‑1 (MQ=255)
                                                                                                                         acacCAGCGTCCACGCTCATTACATCACAATGCTGTTTAATGGCATCGATTGCAGGCAGAAAACGCGCACGTTGCAGGCCATTTCGATAAAGTTCATCCGGCGGAATATTTGACGTCGCTACCAGGGTAATACCgcgagc           >  2:336393/1‑140 (MQ=255)
                                                                                                                         acacCAGCGTCCACGCTCATTACATCACAATGCTGTTTAATGGCATCGATTGCAGGCAGAAAACGCGCACGTTGCAGGCCATTTCGATAAAGTTCATCCGGCGGAATATTTGACGTCGCTACCAGGGTAATACCgcgagc           >  1:660590/1‑140 (MQ=255)
                                                                                                                         acacCAGCGTCCACGCTCATTACATCACAATGCTGTTTAATGGCATCGATTGCAGGCAGAAAACGCGCACGTTGCAGGCCATTTCGATAAAGTTCATCCGGCGGAATATTTGACGTCGCTACCAGGGTAATACCgcgagc           >  2:606038/1‑140 (MQ=255)
                                                                                                                           acCAGCGTCCACGCTCATTACATCACAATGCTGTTTAATGGCATCGATTGCAGGCAGAAAACGCGCACGTTGCAGGCCATTTCGATAAAGTTCATCCGGCGGAATATTTGACGTCGCTACCAGGGTAATACCgcgagcga         >  1:479190/1‑140 (MQ=255)
                                                                                                                            ccAGCGTCCACGCTCATTACATCACAATGCTGTTTAATGGCATCGATTGCAGGCAGAAAACGCGCACGTTGCAGGCCATTTCGATAAAGTTCATCCGGCGGAATATTTGACGTCGCTACCAGGGTAATACCGCGAGCGaa        <  2:115012/140‑1 (MQ=255)
                                                                                                                               gCGTCCACGCTCATTACATCACAATGCTGTTTAATGGCATCGATTGCAGGCAGAAAACGCGCACGTTGCAGGCCATTTCGATAAAGTTCATCCGGCGGAATATTTGACGTCGCTACCAGGGTAATACCGCGAGCGAACAg     >  2:408016/1‑140 (MQ=255)
                                                                                                                                  tCCACGCTCATTACATCACAATGCTGTTTAATGGCATCGATTGCAGGCAGAAAACGCGCACGTTGCAGGCCATTTCGATAAAGTTCATCCGGCGGAATATTTGACGTCGCTACCAGGGTAATACCGCGAGCGAACAGGGc  <  2:479190/140‑1 (MQ=255)
                                                                                                                                  tCCACGCTCATTACATCACAATGCTGTTTAATGGCATCGATTGCAGGCAGAAAACGCGCACGTTGCAGGCCATTTCGATAAAGTTCATCCGGCGGAATATTTGACGTCGCTACCAGGGTAATACCGCGAGCGAACAGGGc  <  2:1129568/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                     
TGAATTTTCTCGTTTCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACGTTCATTACATCACAATGCTGTTTAATGGCATCGATTGCAGGCAGAAAACGCGCACGTTGCAGGCCATTTCGATAAAGTTCATCCGGCGGAATATTTGACGTCGCTACCAGGGTAATACCGCGAGCGAACAGGGC  >  NC_000913/3379245‑3379514

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑