Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,411,831	T→C	L60P (CTG→CCG)	yhdJ →	DNA adenine methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,411,831	0	T	C	100.0%	311.8 / NA	84	L60P (CTG→CCG)	yhdJ	DNA adenine methyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (41/43);  total (41/43)

AAGACCATTATTCACGGTGATGCGCTTGCCGAACTTAAAAAGATCCCCGCCGAAAGTGTCGATCTGATCTTTGCCGACCCACCATATAACATCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCTGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGAACAGTACGGAAAACATGCCCTTTATCGATCTCCAGTGCCGCAAGCTTTTTACCAT  >  NC_000913/3411695‑3411963                                                                                                                                         |                                                                                                                                     aaGACCATTATTCACGGTGATGCGCTTGCCGAACTTAAAAAGATCCCCGCCGAAAGTGTCGATCTGATCTTTGCCGACCCACCATATAACATCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCCGtt                                                                                                                                   >  2:33158/1‑140 (MQ=255)          attCACGGTGATGCGCTTGCCGAACTTAAAAAGATCCCCGCCGAAAGTGTCGATCTGATCTTTGCCGACCCACCATATAACATCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCCGTTTATCGACTg                                                                                                                          <  2:881700/140‑1 (MQ=255)           ttCACGGTGATGCGCTTGCCGAACTTAAAAAGATCCCCGCCGAAAGTGTCGATCTGATCTTTGCCGACCCACTATATAACATCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCCGTTTATCGACTgg                                                                                                                         <  2:1052850/140‑1 (MQ=255)              aCGGTGATGCGCTTGCCGAACTTAAAAAGATCCCCGCCGAAAGTGTCGATCTGATCTTTGCCGACCCACCATATAACATCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCCGTTTATCGACTGGCTg                                                                                                                      >  1:538030/1‑140 (MQ=255)                ggTGATGCGCTTGCCGAACTTAAAAAGATCCCCGCCGAAAGTGTCGATCTGATCTTTGCCGACCCACCATATAACATCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCCGTTTATCGACTg                                                                                                                          >  1:723057/1‑134 (MQ=255)                ggTGATGCGCTTGCCGAACTTAAAAAGATCCCCGCCGAAAGTGTCGATCTGATCTTTGCCGACCCACCATATAACATCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCCGTTTATCGACTg                                                                                                                          <  2:723057/134‑1 (MQ=255)                 gTGATGCGCTTGCCGAACTTAAAAAGATCCCCGCCGAAAGTGTCGATCTGATCTTTGCCGACCCACCATATAACATCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCCGTTTATCGACTGGCTGttt                                                                                                                   <  1:283117/140‑1 (MQ=255)                 gTGATGCACTTGCCGAACTTAAAAAGATCCCCGCCGAAAGTGTCGATCTGATCTTTGCCGACCCACCATATAACATCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCCGTTTATCGACTGGCTGttt                                                                                                                   <  2:400581/140‑1 (MQ=255)                   gATGCGCTTGCCGAACTTAAAAAGATCCCCGCCGAAAGTGTCGATCTGATCTTTGCCGACCCACCATATAACATCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCCGTTTATCGACTGGCTGTTTGa                                                                                                                 <  1:575257/140‑1 (MQ=255)                   gATGCGCTTGCCGAACTTAAAAAGATCCCCGCCGAAAGTGTCGATCTGATCTTTGCCGACCCACCATATAACATCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCCGTTTATCGACTGGCTGTTTGa                                                                                                                 <  1:166891/140‑1 (MQ=255)                    aTGCGCTTGCCGAACTTAAAAAGATCCCCGCCGAAAGTGTCGATCTGATCTTTGCCGACCCACCATATAACATCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCCGTTTATCGACTGGCTGTTTGaa                                                                                                                >  1:1110471/1‑140 (MQ=255)                     tGCGCTTGCCGAACTTAAAAAGATCCCCGCCGAAAGTGTCGATCTGATCTTTGCCGACCCACCATATAACATCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCCGTTTATCGACTGGCTGTTTGAAg                                                                                                               <  1:33158/140‑1 (MQ=255)                     tGCGCTTGCCGAACTTAAAAAGATCCCCGCCGAAAGTGTCGATCTGATCTTTGCCGACCCACCATATAACATCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCCGTTTATCGACTGGCTGTTTGAAg                                                                                                               >  1:830698/1‑140 (MQ=255)                     tGCGCTTGCCGAACTTAAAAAGATCCCCGCCGAAAGTGTCGATCTGATCTTTGCCGACCCACCATATAACATCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCCGTTTATCGACTGGCTGTTTGAAg                                                                                                               >  1:405717/1‑140 (MQ=255)                     tGCGCTTGCCGAACTTAAAAAGATCCCCGCCGAAAGTGTCGATCTGATCTTTGCCGACCCACCATATAACATCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCCGTTTATCGACTGGCTGTTTGAAg                                                                                                               >  1:35497/1‑140 (MQ=255)                     tGCGCTTGCCGAACTTAAAAAGATCCCCGCCGAAAGTGTCGATCTGATCTTTGCCGACCCACCATATAACATCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCCGTTTATCGACTGGCTGTTTGAAg                                                                                                               >  2:274422/1‑140 (MQ=255)                            gCCGAACTTAAAAAGATCCCCGCCGAAAGTGTCGATCTGATCTTTGCCGACCCACCATATAACATCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCCGTTTATCGACTGGCTGTTTGAAGTGATTGc                                                                                                        >  2:831902/1‑140 (MQ=255)                             ccGAACTTAAAAAGATCCCCGCCGAAAGTGTCGATCTGATCTTTGCCGACCCACCATATAACATCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCCGTTTATCGACTGGCTGTTTGAAGTGATTGCa                                                                                                       <  1:775680/140‑1 (MQ=255)                                               ccgccgAAAGTGTCGATCTGATCTTTGCCGACCCACCATATAACATCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCCGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGCCACCGCGTTCTg                                                                                     >  2:1115571/1‑140 (MQ=255)                                                         tgtCGATCTGATCTTTGCCGACCCACCATATAACATCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCCGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAgg                                                                           <  2:1033860/140‑1 (MQ=255)                                                         tgtCGATCTGATCTTTGCCGACCCACCATATAACATCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCCGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAgg                                                                           <  1:183644/140‑1 (MQ=255)                                                         tgtCGATCTGATCTTTGCCGACCCACCATATAACATCGGT‑AAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCCGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAggg                                                                          <  2:223799/140‑1 (MQ=255)                                                          gtCGATCTGATCTTTGCCGACCCACCATATAACATCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCCGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAggg                                                                          <  2:538030/140‑1 (MQ=255)                                                          gtCGATCTGATCTTTGCCGACCCACCATATAACATCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCCGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAggg                                                                          <  1:1102705/140‑1 (MQ=255)                                                          gtCGATCTGATCTTTGCCGACCCACCATATAACATCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCCGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAggg                                                                          <  1:635940/140‑1 (MQ=255)                                                          gtCGATCTGATCTTTGCCGACCCACCATATAACATCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCCGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAggg                                                                          <  1:1115571/140‑1 (MQ=255)                                                          gtCGATCTGATCTTTGCCGACCCACCATATAACATCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCCGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAggg                                                                          <  2:35497/140‑1 (MQ=255)                                                          gtCGATCTGATCTTTGCCGACCCACCATATAACATCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCCGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAggg                                                                          <  2:314256/140‑1 (MQ=255)                                                           tCGATCTGATCTTTGCCGACCCACCATATAACATCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCCGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGgc                                                                         <  1:831902/140‑1 (MQ=255)                                                           tCGATCTGATCTTTGCCGACCCACCATATAACATCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCCGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGgc                                                                         >  2:336021/1‑140 (MQ=255)                                                           tCGATCTGATCTTTGCCGACCCACCATATAACATCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCCGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGgc                                                                         >  2:361944/1‑140 (MQ=255)                                                           tCGATCTGATCTTTGCCGACCCACCATATAACATCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCCGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGgc                                                                         >  1:1073456/1‑140 (MQ=255)                                                           tCGATCTGATCTTTGCCGACCCACCATATAACATCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCCGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGgc                                                                         <  1:269414/140‑1 (MQ=255)                                                            cGATCTGATCTTTGCCGACCCACCATATAACATCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCCGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGCAACCGCGTTCTGAAAAAGCAGGgca                                                                        <  1:332642/140‑1 (MQ=255)                                                             gatctgatctTTGCCGACCCACCATATAACATCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCCGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGgcag                                                                       <  2:740936/140‑1 (MQ=255)                                                                  gatctTTGCCGACCCACCATATAACATCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCCGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGt                                                                  <  2:57595/140‑1 (MQ=255)                                                                     ctTTGCCGACCCACCATATAACATCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCCGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTAca                                                               >  1:16971/1‑140 (MQ=255)                                                                       ttGCCGACCCACCATATAACATCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCCGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTAcatc                                                             <  2:830698/140‑1 (MQ=255)                                                                          ccGACCCACCATATAACATCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCCGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATATACATCATg                                                          <  1:336021/140‑1 (MQ=255)                                                                            gACCCACCATATAACATCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCCGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGaa                                                        <  2:1142301/140‑1 (MQ=255)                                                                            gACCCACCATATAACATCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCCGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGaa                                                        <  1:274422/140‑1 (MQ=255)                                                                             aCCCACCATATAACATCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCCGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGAAc                                                       >  1:445735/1‑140 (MQ=255)                                                                              cccACCATATAACATCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCCGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGAACa                                                      >  1:1134205/1‑140 (MQ=255)                                                                              cccACCATATAACATCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCCGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGAACa                                                      <  2:1073456/140‑1 (MQ=255)                                                                                        aaCATCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCCGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGAACAGTACGGaaaa                                            >  1:647563/1‑140 (MQ=255)                                                                                         aCATCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCCGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGAACAGTACGGAAAAc                                           <  1:842385/140‑1 (MQ=255)                                                                                           aTCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCCGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGAACAGTACGGAAAACAt                                         >  1:692617/1‑140 (MQ=255)                                                                                            tCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCCGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGAACAGTACGGAAAACATg                                        <  2:647563/140‑1 (MQ=255)                                                                                             cGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCCGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGaa                                                        <  1:162181/123‑1 (MQ=255)                                                                                             cGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCCGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGaa                                                        >  2:162181/1‑123 (MQ=255)                                                                                             cGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCCGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGAACAGTACGGAAAACATg                                        <  2:746413/139‑1 (MQ=255)                                                                                             cGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCCGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGAACAGTACGGAAAACATg                                        >  2:740161/1‑139 (MQ=255)                                                                                             cGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCCGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGAACAGTACGGAAAACATg                                        >  1:746413/1‑139 (MQ=255)                                                                                             cGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCCGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGAACAGTACGGAAAACATg                                        <  1:740161/139‑1 (MQ=255)                                                                                             cGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCCGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGAACAGTACGGAAAACATGc                                       >  2:913231/1‑140 (MQ=255)                                                                                             cGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCCGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGAACAGTACGGAAAACATGc                                       >  2:771000/1‑140 (MQ=255)                                                                                             cGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCCGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGAACAGTACGGAAAACATGc                                       >  2:593310/1‑140 (MQ=255)                                                                                             cGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCCGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGAACAGTACGGAAAACATGc                                       >  2:734248/1‑140 (MQ=255)                                                                                             cGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCCGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGAACAGTACGGAAAACATGc                                       >  1:65389/1‑140 (MQ=255)                                                                                               gTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCCGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGAACAGTACGGAAAACATg                                        >  1:1032522/1‑137 (MQ=255)                                                                                               gTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCCGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGAACAGTACGGAAAACATg                                        <  2:1032522/137‑1 (MQ=255)                                                                                               gTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCCGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGAACAGTACGGAAAACATGccc                                     >  1:338131/1‑140 (MQ=255)                                                                                               gTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCCGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGAACAGTACGGAAAACATGccc                                     >  1:990757/1‑140 (MQ=255)                                                                                               gTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCCGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGAACAGCACGGAAAACATGccc                                     >  2:1100452/1‑140 (MQ=255)                                                                                                tAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCCGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGAACAg                                                     <  2:163041/123‑1 (MQ=255)                                                                                                tAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCCGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGAACAg                                                     >  1:163041/1‑123 (MQ=255)                                                                                                    aaTTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCCGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGAACAGTACGGAAAACATGCCCTTTAt                                <  2:1110471/140‑1 (MQ=255)                                                                                                      ttttGATGGTCTGATCGAAGCCTGGAAAGAAGATCCGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGAACAGTACGGAAAACATGCCCTTTATCg                              <  1:840358/140‑1 (MQ=255)                                                                                                                    tCGAAGCCTGGAAAGAAGATCCGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGAACAGTACGGAAAACATGCCCTTTATCGATCTCCAGTGCCGc                <  1:1134087/140‑1 (MQ=255)                                                                                                                        aGCCTGGAAAGAAGATCCGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGAACAGTACGGAAAACATGCCCTTTATCGATCTCCAGTGCCGc                <  2:351298/136‑1 (MQ=255)                                                                                                                        aGCCTGGAAAGAAGATCCGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGAACAGTACGGAAAACATGCCCTTTATCGATCTCCAGTGCCGc                >  1:351298/1‑136 (MQ=255)                                                                                                                        aGCCTGGAAAGAAGATCCGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGAACAGTACGGAAAACATGCCCTTTATCGATCTCCAGTGCCGCAAGc            >  2:170551/1‑140 (MQ=255)                                                                                                                        aGCCTGGAAAGAAGATCCGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGAACAGTACGGAAAACATGCCCTTTATCGATCTCCAGTGCCGCAAGc            >  2:151486/1‑140 (MQ=255)                                                                                                                             ggAAAGAAGATCCGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGAACAGTACGGAAAACATGCCCTTTATCGATCTCCAGTGCCGc                >  1:14085/1‑131 (MQ=255)                                                                                                                             ggAAAGAAGATCCGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGAACAGTACGGAAAACATGCCCTTTATCGATCTCCAGTGCCGc                <  2:14085/131‑1 (MQ=255)                                                                                                                             ggAAAGAAGATCCGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGAACAGTACGGAAAACATGCCCTTTATCGATCTCCAGTGCCGCAAg             >  1:614595/1‑134 (MQ=255)                                                                                                                             ggAAAGAAGATCCGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGAACAGTACGGAAAACATGCCCTTTATCGATCTCCAGTGCCGCAAg             <  2:614595/134‑1 (MQ=255)                                                                                                                             ggAAAGAAGATCCGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGAACAGTACGGAAAACATGCCCTTTATCGATCTCCAGTGCCGCAAGCttttt       >  1:446336/1‑140 (MQ=255)                                                                                                                             ggAAAGAAGATCCGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGAACAGTACGGAAAACATGCCCTTTATCGATCTCCAGTGCCGCAAGCttttt       >  2:1117398/1‑140 (MQ=255)                                                                                                                             ggAAAGAAGATCCGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGAACAGTACGGAAAACATGCCCTTTATCGATCTCCAGTGCCGCAAGCttttt       <  2:761785/140‑1 (MQ=255)                                                                                                                             ggAAAGAAGATCCGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGAACAGTACGGAAAACATGCCCTTTATCGATCTCCAGTGCCGCAAGCttttt       >  1:761785/1‑140 (MQ=255)                                                                                                                             ggAAAGAAGATCCGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGAACAGTACGGAAAACATGCCCTTTATCGATCTCCAGTGCCGCAAGCttttt       >  2:432394/1‑140 (MQ=255)                                                                                                                              gAAAGAAGATCCGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGAACAGTACGGAAAACATGCCCTTTATCGATCTCCAGTGCCGCAAGCTTTTTa      <  1:802471/140‑1 (MQ=255)                                                                                                                                  gaagaTCCGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGAACAGTACGGAAAACATGCCCTTTATCGATCTCCAGTGCCGCAAGCTTTTTACCAt  >  1:942452/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                     AAGACCATTATTCACGGTGATGCGCTTGCCGAACTTAAAAAGATCCCCGCCGAAAGTGTCGATCTGATCTTTGCCGACCCACCATATAACATCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCTGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGAACAGTACGGAAAACATGCCCTTTATCGATCTCCAGTGCCGCAAGCTTTTTACCAT  >  NC_000913/3411695‑3411963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑