Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,476,531	T→C	intergenic (‑91/+76)	yheO ← / ← fkpA	putative DNA‑binding transcriptional regulator YheO/peptidyl‑prolyl cis‑trans isomerase FkpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,476,531	0	T	C	100.0%	100.5 / NA	28	intergenic (‑91/+76)	yheO/fkpA	putative DNA‑binding transcriptional regulator YheO/peptidyl‑prolyl cis‑trans isomerase FkpA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/14);  total (14/14)

GTAAATCCAACTCACTGGTTTCGTTGGTTAAAAGCGACCTGGACATGAAAAAAAACACTCCTTTTCAGGAGCCTGTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGC  >  NC_000913/3476395‑3476667                                                                                                                                         |                                                                                                                                         gTAAATCCAACTCACTGGTTTCGTTGGTTAAAAGCGACCTGGACATGAAAAAAAACACTCCTTTTCAGGAGCCTGTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGCTcc                                                                                                                                       >  1:1132763/1‑140 (MQ=255)  tAAATCCAACTCACTGGTTTCGTTGGTTAAAAGCGACCTGGACATGAAAAAAAACACTCCTTTTCAGGAGCCTGTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGCTCCg                                                                                                                                      <  2:201591/140‑1 (MQ=255)      tCCAACTCACTGGTTTCGTTGGTTAAAAGCGACCTGGACATGAAAAAAAACACTCCTTTTCAGGAGCCTGTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGCTCCGCttt                                                                                                                                  >  2:756683/1‑140 (MQ=255)        cAACTCACTGGTTTCGTTGGTTAAAAGCGACCTGGACATGAAAAAAAACACTCCTTTTCAGGAGCCTGTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGCTCCGCTTTcc                                                                                                                                <  2:851673/140‑1 (MQ=255)            tCACTGGTTTCGTTGGTTAAAAGCGACCTGGACATGAAAAAAAACACTCCTTTTCAGGAGCCTGTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGCTCCg                                                                                                                                      <  1:444285/130‑1 (MQ=255)            tCACTGGTTTCGTTGGTTAAAAGCGACCTGGACATGAAAAAAAACACTCCTTTTCAGGAGCCTGTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGCTCCg                                                                                                                                      >  2:444285/1‑130 (MQ=255)            tCACTGGTTTCGTTGGTTAAAAGCGACCTGGACATGAAAAAAAACACTCCTTTTCAGGAGCCTGTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGCTCCGCTTTCCAGCa                                                                                                                            >  2:719182/1‑140 (MQ=255)            tCACTGGTTTCGTTGGTTAAAAGCGACCTGGACATGAAAAAAAACACTCCTTTTCAGGAGCCTGTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGCTCCGCTTTCCAGCa                                                                                                                            <  1:806699/140‑1 (MQ=255)              aCTGGTTTCGTTGGTTAAAAGCGACCTGGACATGAAAAAAAACACTCCTTTTCAGGAGCCTGTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGCTCCGCTTTCCAGCACt                                                                                                                          >  2:513369/1‑140 (MQ=255)                 ggTTTCGTTGGTTAAAAGCGACCTGGACATGAAAAAAAACACTCCTTTTCAGGAGCCTGTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGCTCCGCTTTCCAGCACTAAt                                                                                                                       <  2:356937/140‑1 (MQ=255)                 ggTTTCGTTGGTTAAAAGCGACCTGGACATGAAAAAAAACACTCCTTTTCAGGAGCCTGTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGCTCCGCTTTCCAGCACTAAt                                                                                                                       <  2:338581/140‑1 (MQ=255)                           gTTAAAAGCGACCTGGACATGAAAAAAAACACTCCTTTTCAGGAGCCTGTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGCTCCGCTTTCCAGCACTAATTATATCCGGc                                                                                                             <  1:149064/140‑1 (MQ=255)                                               gAAAAAAAACACTCCTTTTCAGGAGCCTGTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGCTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAAccgccg                                                                                         <  1:30058/140‑1 (MQ=255)                                                          cTCCTTTTCAGGAGCCTGTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGCTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAAccgccg                                                                                         <  2:902884/129‑1 (MQ=255)                                                          cTCCTTTTCAGGAGCCTGTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGCTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAAccgccg                                                                                         >  1:902884/1‑129 (MQ=255)                                                            ccTTTTCAGGAGCCTGTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGCTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAggcgg                                                                            <  1:513369/140‑1 (MQ=255)                                                                     gAGCCTGTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGCTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTa                                                                   <  2:1132763/140‑1 (MQ=255)                                                                     gAGCCTGTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGCTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTa                                                                   <  1:1120698/140‑1 (MQ=255)                                                                                                              cGGGTAACTAAATTAATACAGCGGAGGCTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCt                          >  2:1084485/1‑140 (MQ=255)                                                                                                              cGGGTAACTAAATTAATACAGCGGAGGCTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCt                          >  1:176599/1‑140 (MQ=255)                                                                                                              cGGGTAACTAAATTAATACAGCGGAGGCTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCt                          >  2:896829/1‑140 (MQ=255)                                                                                                                             aTACAGCGGAGGCTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCa           >  1:272259/1‑140 (MQ=255)                                                                                                                              tACAGCGGAGGCTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAg          >  1:31586/1‑140 (MQ=255)                                                                                                                                  gCGGAGGCTCCGCTGTCAAGCACTAATAATATCCGGCCTGGAATAAAAAAACCGCCGCAAGGTCAGGAGGCGGTGCTTAATGCTTATTTTTTAGCAGAATCTGCGGCGTTCGCATCAGCTTCCGGCTTTGCATCAGCCta      >  2:418797/1‑139 (MQ=255)                                                                                                                                   cGGAGGCTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTc     >  2:775216/1‑140 (MQ=255)                                                                                                                                    ggAGGCTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCg    >  1:149941/1‑140 (MQ=255)                                                                                                                                      aGGCTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGgc  <  1:896829/140‑1 (MQ=255)                                                                                                                                      aGGCTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGgc  <  1:756683/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         GTAAATCCAACTCACTGGTTTCGTTGGTTAAAAGCGACCTGGACATGAAAAAAAACACTCCTTTTCAGGAGCCTGTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGGAGGTTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGC  >  NC_000913/3476395‑3476667

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑