Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,579,816	T→C	E330E (GAA→GAG)	yhhX ←	putative oxidoreductase YhhX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,579,816	0	T	C	97.0%	231.8 / ‑2.3	67	E330E (GAA→GAG)	yhhX	putative oxidoreductase YhhX
Reads supporting (aligned to +/- strand):  ref base T (0/2);  new base C (33/32);  total (33/34)
Fisher's exact test for biased strand distribution p-value = 4.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

AGTGTGGACCCGATCTGCCTGGGATGATAGAGGGTGAAAATTGACCCCTCTGTTCAAAAAAATTGAACTATTCGAGGGGCCATCAAACTTACTTAGCGAGAGTTACTGTGG‑AGGGAGAGGCTTGCTCAAATCCGCGTTCAAGGATTTCCAGATTGGTAAGAACTTCAGATTCCTTGACGTAATTTGGCGCACCGTGGGTGATGGTTTGATACAACGCATCATAAACGCGCCCGTAATCGCCCATCTCCGGCTTCATCTCTTCTCTGACCG  >  NC_000913/3579680‑3579949                                                                                                                                          |                                                                                                                                      aGTGTGGACCCGATCTGCCTGGGATGATAGAGGGTGAAAATTGACCCCTCTGTTCAAAAAAATTGAACTATTCGAGGGGCCATCAAACTTACTTAGCGAGAGTTACTGTGG‑AGGGAGAGGCTTGCTCAAATCCGCGCTCa                                                                                                                                    <  1:387757/140‑1 (MQ=255)       gACCCGATCTGCCTGGGATGATAGAGGGTGAAAATTGACCCCTCTGTTCAAAAAAATTGAACTATTCGAGGGGCCATCAAACTTACTTAGCGAGAGTTACTGTGG‑AGGGAGAGGCTTGCTCAAATCCGCGCTCAAGGAtt                                                                                                                              >  2:520621/1‑140 (MQ=255)       gACCCGATCTGCCTGGGATGATAGAGGGTGAAAATTGACCCCTCTGTTCAAAAAAATTGAACTATTCGAGGGGCCATCAAACTTACTTAGCGAGAGTTACTGTGG‑AGGGAGAGGCTTGCTCAAATCCGCGCTCAAGGAtt                                                                                                                              >  2:539590/1‑140 (MQ=255)          ccGATCTGCCTGGGATGATAGAGGGTGAAAATTGACCCCTCTGTTCAAAAAAATTGAACTATTCGAGGGGCCATCAAACTTACTTAGCGAGAGTTACTGTGG‑AGGGAGAGGCTTGCTCAAATCCGCGCTCAAGGATTTcc                                                                                                                           <  1:753716/140‑1 (MQ=255)             aTCTGCCTGGGATGATAGAGGGTGAAAATTGACCCCTCTGTTCAAAAAAATTGAACTATTCGAGGGGCCATCAAACTTACTTAGCGAGAGTTACTGTGG‑AGGGAGAGGCTTGCTCAAATCCGCGCTCAAGGATTTccggt                                                                                                                        >  1:38028/1‑137 (MQ=255)                          gatAGAGGGTGAAAATTGACCCCTCTGTTCAAAAAAATTGAACTATTCGAGGGGCCATCAAACTTACTTAGCGAGAGTTACTGTGG‑AGGGAGAGGCTTGCTCAAATCCGCGCTCAAGGATTTCCAGATTGGTAAg                                                                                                                >  2:714969/1‑135 (MQ=255)                          gatAGAGGGTGAAAATTGACCCCTCTGTTCAAAAAAATTGAACTATTCGAGGGGCCATCAAACTTACTTAGCGAGAGTTACTGTGG‑AGGGAGAGGCTTGCTCAAATCCGCGCTCAAGGATTTCCAGATTGGTAAg                                                                                                                <  1:714969/135‑1 (MQ=255)                          gatAGAGGGTGAAAATTGACCCCTCTGTTCAAAAAAATTGAACTATTCGAGGGGCCATCAAACTTACTTAGCGAGAGTTACTGTGG‑AGGGAGAGGCTTGCTCAAATCCGCGCTCAAGGATTTCCAGATTGGTAAGAACtt                                                                                                           >  1:765597/1‑140 (MQ=255)                            tAGAGGGTGAAAATTGACCCCTCTGTTCAAAAAAATTGAACTATTCGAGGGGCCATCAAACTTACTTAGCGAGAGTTACTGTGG‑AGGGAGAGGCTTGCTCAAATCCGCGCTCAAGGATTTCCAGATTGGTAAGAACTTCa                                                                                                         >  1:112219/1‑140 (MQ=255)                            tAGAGGGTGAAAATTGACCCCTCTGTTCAAAAAAATTGAACTATTCGAGGGGCCATCAAACTTACTTAGCGAGAGTTACTGTGG‑AGGGAGAGGCTTGCTCAAATCCGCGCTCAAGGATTTCCAGATTGGTAAGAACTTCa                                                                                                         <  2:913628/140‑1 (MQ=255)                              gagGGTGAAAATTGACCCCTCTGTTCAAAAAAATTGAACTATTCGAGGGGCCATCAAACTTACTTAGCGAGAGTTACTGTGG‑AGGGAGAGGCTTGCTCAAATCCGCGTTCAAGGATTTCCAGATTGGTAAGAACTTCAGa                                                                                                       <  2:437835/140‑1 (MQ=255)                                 ggTGAAAATTGACCCCTCTGTTCAAAAAAATTGAACTATTCGAGGGGCCATCAAACTTACTTAGCGAGAGTTACTGTGG‑AGGGAGAGGCTTGCTCAAATCCGCGCTCAAGGATTTCCAGATTGGTAAGAACTTCAGATTc                                                                                                    >  2:20733/1‑140 (MQ=255)                                 ggTGAAAATTGACCCCTCTGTTCAAAAAAATTGAACTATTCGAGGGGCCATCAAACTTACTTAGCGAGAGTTACTGTGG‑AGGGAGAGGCTTGCTCAAATCCGCGCTCAAGGATTTCCAGATTGGTAAGAACTTCAGATTc                                                                                                    >  1:762434/1‑140 (MQ=255)                                 ggTGAAAATTGACCCCTCTGTTCAAAAAAATTGAACTATTCGAGGGGCCATCAAACTTACTTAGCGAGAGTTACTGTGG‑AGGGAGAGGCTTGCTCAAATCCGCGCTCAAGGATTTCCAGATTGGTAAGAACTTCAGATTc                                                                                                    >  2:60165/1‑140 (MQ=255)                                   tGAAAATTGACCCCTCTGTTCAAAAAAATTGAACTATTCGAGGGGCCATCAAACTTACTTAGCGAGAGTTACTGTGG‑AGGGAGAGGCTTGCTCAAATCCGCGCTCAAGGATTTCCAGATTGGTAAGAACTTCAGATTCCt                                                                                                  <  2:53042/140‑1 (MQ=255)                                     aaaaTTGACCCCTCTGTTCAAAAAAATTGAACTATTCGAGGGGCCATCAAACTTACTTAGCGAGAGTTACTGTGG‑AGGGAGAGGCTTGCTCAAATCCGCGCTCAAGGATTTCCAGATTGGTAAGAACTTCAGATTCCTTg                                                                                                >  2:392560/1‑140 (MQ=255)                                      aaaTTGACCCCTCTGTTCAAAAAAATTGAACTATTCGAGGGGCCATCAAACTTACTTAGCGAGAGTTACTGTGG‑AGGGAGAGGCTTGCTCAAATCCGCGCTCAAGGATTTCCAGATTGGTAAGAACTTCAGATTCCTTGa                                                                                               <  1:539590/140‑1 (MQ=255)                                         ttGACCCCTCTGTTCAAAAAAATTGAACTATTCGAGGGGCCATCAAACTTACTTAGCGAGAGTTACTGTGG‑AGGGAGAGGCTTGCTCAAATCCGCGCTCAAGGATTTCCAGATTGGTAAGAACTTCAGATTCCTTGACGt                                                                                            >  2:270340/1‑140 (MQ=255)                                            aCCCCTCTGTTCAAAAAAATTGAACTATTCGAGGGGCCATCAAACTTACTTAGCGAGAGTTACTGTGG‑AGGGAGAGGCTTGCTCAAATCCGCGCTCAAGGATTTCCAGATTGGTAAGAACTTCa                                                                                                         <  1:773193/124‑1 (MQ=255)                                            aCCCCTCTGTTCAAAAAAATTGAACTATTCGAGGGGCCATCAAACTTACTTAGCGAGAGTTACTGTGG‑AGGGAGAGGCTTGCTCAAATCCGCGCTCAAGGATTTCCAGATTGGTAAGAACTTCa                                                                                                         >  2:773193/1‑124 (MQ=255)                                            aCCCCTCTGTTCAAAAAAATTGAACTATTCGAGGGGCCATCAAACTTACTTAGCGAGAGTTACTGTGG‑AGGGAGAGGCTTGCTCAAATCCGCGCTCAAGGATTTCCAGATTGGTAAGAACTTCAGATTCCTTGACGTAAt                                                                                         >  1:528570/1‑140 (MQ=255)                                             ccccTCTGTTCAAAAAAATTGAACTATTCGAGGGGCCATCAAACTTACTTAGCGAGAGTTACTGTGG‑AGGGAGAGGCTTGCTCAAATCCGCGCTCAAGGATTTCCAGATTGGTAAGAACTTCAGATTCCTTGACGTAAtt                                                                                        >  1:1121010/1‑140 (MQ=255)                                                 tctGTTCAAAAAAATTGAACTATTCGAGGGGCCATCAAACTTACTTAGCGAGAGTTACTGTGG‑AGGGAGAGGCTTGCTCAAATCCGCGCTCAAGGATTTCCAGATTGGTAAGAACTTCAGATTCCTTGACGTAATTTGgc                                                                                    >  1:587509/1‑140 (MQ=255)                                                 ctgtttaaaaaaaaTTGACTAATTCGGGGGGCCTTCAACCTTCCTAAGC‑AGAATTTCTTTGGAAGGGGGAGCCTTGCCCAATTCCCCG‑TCAAAGAATTTCAGATTGGTAAGAACTTCAGATTCCTTGACGTAATTTGgcg                                                                                   <  2:38028/133‑1 (MQ=255)                                                  ctGTTCAAAAAAATTGAACTATTCGAGGGGCCATCAAACTTACTTAGCGAGAGTTACTGTGG‑AGGGAGAGGCTTGCTCAAATCCGCGCTCAAGGATTTCCAGATTGGTAAGAACTTCAGATTCCTTGACGTAATTTGgcg                                                                                   <  1:1110079/140‑1 (MQ=255)                                                  ctGTTCAAAAAAATTGAACTATTCGAGGGGCCATCAAACTTACTTAGCGAGAGTTACTGTGG‑AGGGAGAGGCTTGCTCAAATCCGCGCTCAAGGATTTCCAGATTGGTAAGAACTTCAGATTCCTTGACGTAATTTGgcg                                                                                   <  1:270340/140‑1 (MQ=255)                                                  ctGTTCAAAAAAATTGAACTATTCGAGGGGCCATCAAACTTACTTAGCGAGAGTTACTGTGG‑AGGGAGAGGCTTGCTCAAATCCGCGCTCAAGGATTTCCAGATTGGTAAGAACTTCAGATTCCTTGACGTAATTTGgcg                                                                                   <  2:367953/140‑1 (MQ=255)                                                    gTTCAAAAAAATTGAACTATTCGAGGGGCCATCAAACTTACTTAGCGAGAGTTACTGTGG‑AGGGAGAGGCTTGCTCAAATCCGCGCTCAAGGATTTCCAGATTGGTAAGAACTTCAGATTCCTTGACGTAATTTGGCGCa                                                                                 <  1:60165/140‑1 (MQ=255)                                                      tCAAAAAAATTGAACTATTCGAGGGGCCATCAAACTTACTTAGCGAGAGTTACTGTGG‑AGGGAGAGGCTTGCTCAAATCCGCGCTCAAGGATTTCCAGATTGGTAAGAACTTCAGATTCCTTGACGTAATTTGGCGCAcc                                                                               <  1:241884/140‑1 (MQ=255)                                                      tCAAAAAAATTGAACTATTCGAGGGGCCATCAAACTTACTTAGCGAGAGTTACTGTGG‑AGGGAGAGGCTTGCTCAAATCCGCGCTCAAGGATTTCCAGATTGGTAAGAACTTCAGATTCCTTGACGTAATTTGGCGCAcc                                                                               <  1:1116065/140‑1 (MQ=255)                                                          aaaaaTTGAACTATTCGAGGGGCCATCAAACTTACTTAGCGAGAGTTACTGTGG‑AGGGAGAGGCTTGCTCAAATCCGCGCTCAAGGATTTCCAGATTGGTAAGAACTTCAGATTCCTTGACGTAATTTGGCGCACCGTgg                                                                           >  2:226274/1‑140 (MQ=255)                                                          aaaaaTTGAACTATTCGAGGGGCCATCAAACTTACTTAGCGAGAGTTACTGTGG‑AGGGAGAGGCTTGCTCAAATCCGCGCTCAAGGATTTCCAGATTGGTAAGAACTTCAGATTCCTTGACGTAATTTGGCGCACCGTgg                                                                           >  2:286767/1‑140 (MQ=255)                                                             aaTTGAACTATTCGAGGGGCCATCAAACTTACTTAGCGAGAGTTACTGTGG‑AGGGAGAGGCTTGCTCAAATCCGCGCTCAAGGATTTCCAGATTGGTAAGAACTTCAGATTCCTTGACGTAATTTGGCGCACCGTGGGTg                                                                        >  2:370462/1‑140 (MQ=255)                                                              aTTGAACTATTCGAGGGGCCATCAAACTTACTTAGCGAGAGTTACTGTGG‑AGGGAGAGGCTTGCTCAAATCCGCGCTCAAGGATTTCCAGATTGGTAAGAACTTCAGATTCCTTGACGTAATTTGGCGCACCGTGGGTGa                                                                       <  1:95514/140‑1 (MQ=255)                                                              aTTGAACTATTCGAGGGGCCATCAAACTTACTTAGCGAGAGTTACTGTGG‑AGGGAGAGGCTTGCTCAAATCCGCGCTCAAGGATTTCCAGATTGGTAAGAACTTCAGATTCCTTGACGTAATTTGGCGCACCGTGGGTGa                                                                       <  1:392560/140‑1 (MQ=255)                                                                        tCGAGGGGCCATCAAACTTACTTAGCGAGAGTTACTGTGG‑AGGGAGAGGCTTGCTCAAATCCGCGCTCAAGGATTTCCAGATTGGTAAGAACTTCAGATTCCTTGACGTAATTTGGCGCACCGTGGGTGATGGTTTGATa                                                             <  2:765597/140‑1 (MQ=255)                                                                         cGAGGGGCCATCAAACTTACTTAGCGAGAGTTACTGTGG‑AGGGAGAGGCTTGCTCAAATCCGCGCTCAAGGATTTCCAGATTGGTAAGAACTTCAGATTCCTTGACGTAATTTGGCGCACCGTGGGTGATGGTTTGATAc                                                            <  2:1121010/140‑1 (MQ=255)                                                                           aGGGGCCATCAAACTTACTTAGCGAGAGTTACTGTGG‑AGGGAGAGGCTTGCTCAAATCCGCGCTCAAGGATTTCCAGATTGGTAAGAACTTCAGATTCCTTGACGTAATTTGGCGCACCGTGGGTGATGGTTTGATACaa                                                          <  2:587509/140‑1 (MQ=255)                                                                             gggCCATCAAACTTACTTAGCGAGAGTTACTGTGG‑AGGGAGAGGCTTGCTCAAATCCGCGCTCAAGGATTTCCAGATTGGTAAGAACTTCAGATTCCTTGACGTAATTTGGCGCACCGTGGGTGATGGTTTGATACAACg                                                        <  1:370462/140‑1 (MQ=255)                                                                             gggCCATCAAACTTACTTAGCGAGAGTTACTGTGG‑AGGGAGAGGCTTGCTCAAATCCGCGCTCAAGGATTTCCAGATTGGTAAGAACTTCAGATTCCTTGACGTAATTTGGCGCACCGTGGGTGATGGTTTGATACAACg                                                        <  1:20733/140‑1 (MQ=255)                                                                               gCCATCAAACTTACTTAGCGAGAGTTACTGTGG‑AGGGAGAGGCTTGCTCAAATCCGCGCTCAAGGATTTCCAGATTGGTAAGAACTTCAGATTCCTTGACGTAATTTGGCGCACCGTGGGTGATGGTTTGATACaa                                                          >  2:33963/1‑136 (MQ=255)                                                                               gCCATCAAACTTACTTAGCGAGAGTTACTGTGG‑AGGGAGAGGCTTGCTCAAATCCGCGCTCAAGGATTTCCAGATTGGTAAGAACTTCAGATTCCTTGACGTAATTTGGCGCACCGTGGGTGATGGTTTGATACaa                                                          <  1:33963/136‑1 (MQ=255)                                                                                 cATCAAACTTACTTAGCGAGAGTTACTGTGG‑AGGGAGAGGCTTGCTCAAATCCGCGCTCAAGGATTTCCAGATTGGTAAGAACTTCAGATTCCTTGACGTAATTTGGCGCACCGTGGGTGATGGtt                                                                  <  2:767948/126‑1 (MQ=255)                                                                                 cATCAAACTTACTTAGCGAGAGTTACTGTGG‑AGGGAGAGGCTTGCTCAAATCCGCGCTCAAGGATTTCCAGATTGGTAAGAACTTCAGATTCCTTGACGTAATTTGGCGCACCGTGGGTGATGGtt                                                                  >  1:767948/1‑126 (MQ=255)                                                                                 cATCAAACTTACTTAGCGAGAGTTACTGTGG‑AGGGAGAGGCTTGCTCAAATCCGCGCTCAAGGATTTCCAGATTGGTAAGAACTTCAGATTCCTTGACGTAATTTGGCGCACCGTGGGTGATGGTTTGATACAACGcatc                                                    >  2:816933/1‑140 (MQ=255)                                                                                 cATCAAACTTACTTAGCGAGAGTTACTGTGG‑AGGGAGAGGCTTGCTCAAATCCGCGCTCAAGGATTTCCAGATTGGTAAGAACTTCAGATTCCTTGACGTAATTTGGCGCACCGTGGGTGATGGTTTGATACAACGcatc                                                    >  2:367051/1‑140 (MQ=255)                                                                                     aaaCTTACTTAGCGAGAGTTACTGTGG‑AGGGAGAGGCTTGCTCAAATCCGCGCTCAAGGATTTCCAGATTGGTAAGAACTTCAGATTCCTTGACGTAATTTGGCGCACCGTGGGTGATGGTTTGATACAACGCATCATaa                                                >  1:979295/1‑140 (MQ=255)                                                                                      aaCTTACTTAGCGAGAGTTACTGTGG‑AGGGAGAGGCTTGCTCAAATCCGCGCTCAAGGATTTCCAGATTGGTAAGAACTTCAGATTCCTTGACGTAATTTGGCGCACCGTGGGTGATGGTTTGATACAACGCATCATaaa                                               >  1:602908/1‑140 (MQ=255)                                                                                           acttaGCGAGAGTTACTGTGG‑AGGGAGAGGCTTGCTCAAATCCGCGCTCAAGGATTTCCAGATTGGTAAGAACTTCAGATTCCTTGACGTAATTTGGCGCACCGTGGGTGATGGTTTGATACAACGCATCATAAACgcgc                                          <  1:226274/140‑1 (MQ=255)                                                                                            cttaGCGAGAGTTACTGTGG‑AGGGAGAGGCTTGCTCAAATCCGCGCTCAAGGATTTCCAGATTGGTAAGAACTTCAGATTCCTTGACGTAATTTGGCGCACCGTGGGTGATGGTTTGATACAACGCATCATAAACGCGcc                                         <  2:762434/140‑1 (MQ=255)                                                                                              taGCGAGAGTTACTGTGG‑AGGGAGAGGCTTGCTCAAATCCGCGTTCAAGGATTTCCAGATTGGTAAGAACTTCAGATTCCTTGACGTAATTTGGCGCACCGTGGGTGATGGTTTGATACAACGCATCATAAACGCGCCCg                                       <  2:1072979/140‑1 (MQ=255)                                                                                               aGCGAGAGTTACTGTGG‑AGGGAGAGGCTTGCTCAAATCCGCGCTCAAGGATTTCCAGATTGGTAAGAACTTCAGATTCCTTGACGTAATTTGGCGCACCGTGGGTGATGGTTTGATACAACGCATCATAAACGCGCCCGt                                      >  1:743387/1‑140 (MQ=255)                                                                                                  gagaGTTACTGTGG‑AGGGAGAGGCTTGCTCAAATCCGCGCTCAAGGATTTCCAGATTGGTAAGAACTTCAGATTCCTTGACGTAATTTGGCGCACCGTGGGTGATGGTTTGATACAACGCATCATAAACGCGCCCGTAAt                                   >  1:780864/1‑140 (MQ=255)                                                                                                  gagaGTTACTGTGG‑AGGGAGAGGCTTGCTCAAATCCGCGCTCAAGGATTTCCAGATTGGTAAGAACTTCAGATTCCTTGACGTAATTTGGCGCACCGTGGGTGATGGTTTGATACAACGCATCATAAACGCGCCCGTAAt                                   >  1:357713/1‑140 (MQ=255)                                                                                                      gTTACTGTGG‑AGGGAGAGGCTTGCTCAAATCCGCGCTCAAGGATTTCCAGATTGGTAAGAACTTCAGATTCCTTGACGTAATTTGGCGCACCGTGGGTGATGGTTTGATACAACGCATCATAAACGCGCCCGTAATCGcc                               <  1:196900/140‑1 (MQ=255)                                                                                                       ttACTGTGG‑AGGGAGAGGCTTGCTCAAATCCGCGCTCAAGGATTTCCAGATTGGTAAGAACTTCAGATTCCTTGACGTAATTTGGCGCACCGTGGGTGATGGTTTGATACAACGCATCATAAACGCGCCCGTAATCGccc                              <  2:979295/140‑1 (MQ=255)                                                                                                           tgtgG‑AGGGAGAGGCTTGCTCAAATCCGCGCTCAAGGATTTCCAGATTGGTAAGAACTTCAGATTCCTTGACGTAATTTGGCGCACCGTGGGTGATGGTTTGATACAACGCATCATAAACGCGCCCGTAATCGCCCAtct                          <  2:112219/140‑1 (MQ=255)                                                                                                              gg‑agggagAGGCTTGCTCAAATCCGCGCTCAAGGATTTCCAGATTGGTAAGAACTTCAGATTCCTTGACGTAATTTGGCGCACCGTGGGTGATGGTTTGATACAACGCATCATAAACGCGCCCGTAATCGCCCATCTCCg                       >  2:748183/1‑140 (MQ=255)                                                                                                              gg‑agggagAGGCTTGCTCAAATCCGCGCTCAAGGATTTCCAGATTGGTAAGAACTTCAGATTCCTTGACGTAATTTGGCGCACCGTGGGTGATGGTTTGATACAACGCATCATAAACGCGCCCGTAATCGCCCATCTCCg                       <  2:602908/140‑1 (MQ=255)                                                                                                                 agggagAGGCTTGCTCAAATCCGCGCTCAAGGATTTCCAGATTGGTAAGAACTTCAGATTCCTTGACGTAATTTGGCGCACCGTGGGTGATGGTTTGATACAACGCATCATAAACGCGCCCGTAATCGCCCATCTCCGGc                     >  1:1077192/1‑140 (MQ=255)                                                                                                                   ggagAGGCTTGCTCAAATCCGCGCTCAAGGATTTCCAGATTGGTAAGAACTTCAGATTCCTTGACGTAATTTGGCGCACCGTGGGTGATGGTTTGATACAACGCATCATAAACGCGCCCGTAATCGCCCATCTCCGGCtt                   <  2:780864/140‑1 (MQ=255)                                                                                                                   ggagAGGCTTGCTCAAATCCGCGCTCAAGGATTTCCAGAGTGGTAAGAACTTCAGATTCCTTGAATTAATTTGGCGCACCGTGGGTGATGGTTTGATACAACGCATCATAAACGCGCCCGTAATCGCCCATCTCCGGCtt                   <  2:439121/140‑1 (MQ=255)                                                                                                                     agagGCTTGCTCAAATCCGCGCTCAAGGATTTCTAGATTGGTAAGAACTTCAGATTCCTTGACGTAATTTGGCGCACCGTGGGTGATGGTTTGATACAACGCATCATAAACGCGCCCGTAATCGCCCATCTCCGGCTTCa                 >  1:1096276/1‑140 (MQ=255)                                                                                                                             gCTCAAATCCGCGCTCAAGGATTTCCAGATTGGTAAGAACTTCAGATTCCTTGACGTAATTTGGCGCACCGTGGGTGATGGTTTGATACAACGCATCATAAACGCGCCCGTAATCGCCCATCTCCGGCTTCAtctcttct         >  1:1083049/1‑140 (MQ=255)                                                                                                                               tCAAATCCGCGCTCAAGGATTTCCAGATTGGTAAGAACTTCAGATTCCTTGACGTAATTTGGCGCACCGTGGGTGATGGTTTGATACAACGCATCATAAACGCGCCCGTAATCGCCCATCTCCGGCTTCAtctcttctct       <  2:1077192/140‑1 (MQ=255)                                                                                                                                cAAATCCGCGCTCAAGGATTTCTAGATTGGTAAGAACTTCAGATTCCTTGACGTAATTTGGCGCACCGTGGGTGATGGTTTGATACAACGCATCATAAACGCGCCCGTAATCGCCCATCTCCGGCTTCATCTCTTCTCTg      <  2:1096276/140‑1 (MQ=255)                                                                                                                                   aTCCGCGCTCAAGGATTTCCAGATTGGTAAGAACTTCAGATTCCTTGACGTAATTTGGCGCACCGTGGGTGATGGTTTGATACAACGCATCATAAACGCGCCCGTAATCGCCCATCTCCGGCTTCAtct              <  2:1129352/129‑1 (MQ=255)                                                                                                                                   aTCCGCGCTCAAGGATTTCCAGATTGGTAAGAACTTCAGATTCCTTGACGTAATTTGGCGCACCGTGGGTGATGGTTTGATACAACGCATCATAAACGCGCCCGTAATCGCCCATCTCCGGCTTCAtct              >  1:1129352/1‑129 (MQ=255)                                                                                                                                    tCCGCGCTCAAGGATTTCCAGATTGGTAAGAACTTCAGATTCCTTGACGTAATTTGGCGCAAGGTGGGTGATGGTTTGATACAACGCATCATAAACGCGCCCGTAATCGCCCATCTCCGGCTTCATCTCTTCTCTGACCg  >  2:1032827/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                      AGTGTGGACCCGATCTGCCTGGGATGATAGAGGGTGAAAATTGACCCCTCTGTTCAAAAAAATTGAACTATTCGAGGGGCCATCAAACTTACTTAGCGAGAGTTACTGTGG‑AGGGAGAGGCTTGCTCAAATCCGCGTTCAAGGATTTCCAGATTGGTAAGAACTTCAGATTCCTTGACGTAATTTGGCGCACCGTGGGTGATGGTTTGATACAACGCATCATAAACGCGCCCGTAATCGCCCATCTCCGGCTTCATCTCTTCTCTGACCG  >  NC_000913/3579680‑3579949

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑