Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,720,192	T→C	G48G (GGT→GGC)	cspA →	cold shock protein CspA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,720,192	0	T	C	100.0%	266.2 / NA	73	G48G (GGT→GGC)	cspA	cold shock protein CspA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (32/41);  total (32/41)

GGTAAAATGACTGGTATCGTAAAATGGTTCAACGCTGACAAAGGCTTCGGCTTCATCACTCCTGACGATGGCTCTAAAGATGTGTTCGTACACTTCTCTGCTATCCAGAACGATGGTTACAAATCTCTGGACGAAGGTCAGAAAGTGTCCTTCACCATCGAAAGCGGCGCTAAAGGCCCGGCAGCTGGTAACGTAACCAGCCTGTAATCTCTGCTTAAAAGCACAGAATCTAAGATCCCTGCCATTTGGCGGGGATTTTTTTATTTGTTTT  >  NC_000913/3720055‑3720325                                                                                                                                          |                                                                                                                                      ggTAAAATGACTGGTATCGTAAAATGGTTCAACGCTGACAAAGGCTTCGGCTTCATCACTCCTGACGATGGCTCTAAAGATGTGTTCGTACACTTCTCTGCTATCCAGAACGATGGTTACAAATCTCTGGACGAAGGCCa                                                                                                                                     <  2:436019/140‑1 (MQ=255)  gTAAAATGACTGGTATCGTAAAATGGTTCAACGCTGACAAAGGCTTCGGCTTCATCACTCCTGACGATGGCTCTAAAGATGTGTTCGTACACTTCTCTGCTATCCAGAACGATGGTTACAAATCTCTGGACGAAGGCCAg                                                                                                                                    >  1:274665/1‑140 (MQ=255)  gTAAAATGACTGGTATCGTAAAATGGTTCAACGCTGACAAAGGCTTCGGCTTCATCACTCCTGACGATGGCTCTAAAGATGTGTTCGTACACTTCTCTGCTATCCAGAACGATGGTTACAAATCTCTGGACGAAGGCCAg                                                                                                                                    >  1:268988/1‑140 (MQ=255)  gTAAAATGACTGGTATCGTAAAATGGTTCAACGCTGACAAAGGCTTCGGCTTCATCACTCCTGACGATGGCTCTAAAGATGTGTTCGTACACTTCTCTGCTATCCAGAACGATGGTTACAAATCTCTGGACGAAGGCCAg                                                                                                                                    >  1:68992/1‑140 (MQ=255)  gTAAAATGACTGGTATCGTAAAATGGTTCAACGCTGACAAAGGCTTCGGCTTCATCACTCCTGACGATGGCTCTAAAGATGTGTTAGTACACTTCTCTGCTATCCAGAACGATGGTTACAAATCTCTGGACGAAGGCCAg                                                                                                                                    >  2:727180/1‑140 (MQ=255)      aaTGACTGGTATCGTAAAATGGTTCAACGCTGACAAAGGCTTCGGCTTCATCACTCCTGACGATGGCTCTAAAGATGTGTTCGTACACTTCTCTGCTATCCAGAACGATGGTTACAAATCTCTGGACGAAGGCCAGAAAg                                                                                                                                >  1:200557/1‑140 (MQ=255)          aCTGGTATCGTAAAATGGTTCAACGCTGACAAAGGCTTCGGCTTCATCACTCCTGACGATGGCTCTAAAGATGTGTTCGTACACTTCTCTGCTATCCAGAACGATGGTTACAAATCTCTGGACGAAGGCCAGAAAGTGTc                                                                                                                            <  1:97906/140‑1 (MQ=255)               tATCGTAAAATGGTTCAACGCTGACAAAGGCTTCGGCTTCATCACTCCTGACGATGGCTCTAAAGATGTGTTCGTACACTTCTCTGCTATCCAGAACGATGGTTACAAATCTCTGGACGAAGGCCAGAAAGTGTCCTTCa                                                                                                                       <  1:1121408/140‑1 (MQ=255)                    tAAAATGGTTCAACGCTGACAAAGGCTTCGGCTTCATCACTCCTGACGATGGCTCTAAAGATGTGTTCGTACACTTCTCTGCTATCCAGAACGATGGTTACAAATCTCTGGACGAAGGCCAGAAAGTGTCCTTCACCATc                                                                                                                  >  1:769335/1‑140 (MQ=255)                         tGGTTCAACGCTGACAAAGGCTTCGGCTTCATCACTCCTGACGATGGCTCTAAAGATGTGTTAGTACACTTCTCTGCTATCCAGAACGATGGTTACAAATCTCTGGACGAAGGCCAGAAAGTGTCCTTCACCATCGAAAg                                                                                                             <  1:727180/140‑1 (MQ=255)                          ggTTCAACGCTGACAAAGGCTTCGGCTTCATCACTCCTGACGATGGCTCTAAAGATGTGTTCGTACACTTCTCTGCTATCCAGAACGATGGTTACAAATCTCTGGACGAAGGCCAGAAAGTGTCCTTCACCATCGAAAgc                                                                                                            <  1:146135/140‑1 (MQ=255)                           gTTCAACGCTGACAAAGGCTACGGCTTCATCACTCCTGACGATGGCTCTAAAGATGTGTTCGTACACTTCTCTGCTATCCAGAACGATGGTTACAAATCTCTGGACGAAGGCCAGAAAGTGTCCTTCACCATCGAAAgcg                                                                                                           <  1:776587/140‑1 (MQ=255)                            ttCAACGCTGACAAAGGCTTCGGCTTCATCACTCCTGACGATGGCTCTAAAGATGTGTTCGTACACTTCTCTGCTATCCAGAACGATGGTTACAAATCTCTGGACGAAGGCCAGAAAGTGTCCTTCACCATCGAAAgcgg                                                                                                          <  1:775543/140‑1 (MQ=255)                             tCAACGCTGACAAAGGCTTCGGCTTCATCACTCCTGACGATGGCTCTAAAGATGTGTTCGTACACTTCTCTGCTATCCAGAACGATGGTTACAAATCTCTGGACGAAGGCTAGAAAGTGTCCTTCACCATCGAAAgcggc                                                                                                         >  2:349366/1‑140 (MQ=255)                                aCGCTGACAAAGGCTTCGGCTTCATCACTCCTGACGATGGCTCTAAAGATGTGTTCGTACACTTCTCTGCTATCCAGAACGATGGTTACAAATCTCTGGACGAAGGCCAGAAAGTGTCCTTCACCATCGAAAGCGGCGCt                                                                                                      <  2:68992/140‑1 (MQ=255)                                           ggcttcggcttcATCACTCCTGACGATGGCTCTAAAGATGTGTTCGTACACTTCTCTGCTATCCAGAACGATGGTTACAAATCTCTGGACGAAGGCCAGAAAGTGTCCTTCACCATCGAAAGCGGCGCTaa                                                                                                    >  1:696900/1‑131 (MQ=255)                                           ggcttcggcttcATCACTCCTGACGATGGCTCTAAAGATGTGTTCGTACACTTCTCTGCTATCCAGAACGATGGTTACAAATCTCTGGACGAAGGCCAGAAAGTGTCCTTCACCATCGAAAGCGGCGCTaa                                                                                                    <  2:696900/131‑1 (MQ=255)                                           ggcttcggcttcATCACTCCTGACGATGGCTCTAAAGATGTGTTCGTACACTTCTCTGCTATCCAGAACGATGGTTACAAATCTCTGGACGAAGGCCAGAAAGTGTCCTTCACCATCGAAAGCGGCGCTAAAGGCCCGGc                                                                                           >  2:1077184/1‑140 (MQ=255)                                              ttcggcttcATCACTCCTGACGATGGCTCTAAAGATGTGTTCGTACACTTCTCTGCTATCCAGAACGATGGTTACAAATCTCTGGACGAAGGCCAGAAAGTGTCCTTCACCATCGAAAGCGGCGCTAAAGGCCCGGCAGc                                                                                        >  1:212662/1‑140 (MQ=255)                                                cggcttcATCACTCCTGACGATGGCTCTAAAGATGTGTTCGTACACTTCTCTGCTATCCAGAACGATGGTTACAAATCTCTGGACGAAGGCCAGAAAGTGTCCTTCACCATCGAAAGCGGCGCTAAAGGCCCGGCAGCTg                                                                                      >  1:695990/1‑140 (MQ=255)                                                    ttcATCACTCCTGACGATGGTTCTAAAGATGTGTTCGTACACTTCTCTGCTATCCAGAACGATGGTTACAAATCTCTGGACGAAGGCCAGAAAGTGTCCTTCACCATCGAAAGCGGCGCTAAAGGCCCGGCAGCTGgtaa                                                                                  <  2:578349/140‑1 (MQ=255)                                                      catcaCTCCTGACGATGGCTCTAAAGATGTGTTCGTACACTTCTCTGCTATCCAGAACGATGGTTACAAATCTCTGGACGAAGGCCAGAAAGTGTCCTTCACCATCGAAAGCGGCGCTAAAGGCCCGGCAGCTGgt                                                                                    >  1:777224/1‑136 (MQ=255)                                                      catcaCTCCTGACGATGGCTCTAAAGATGTGTTCGTACACTTCTCTGCTATCCAGAACGATGGTTACAAATCTCTGGACGAAGGCCAGAAAGTGTCCTTCACCATCGAAAGCGGCGCTAAAGGCCCGGCAGCTGgt                                                                                    <  2:777224/136‑1 (MQ=255)                                                         caCTCCTGACGATGGCTCTAAAGATGTGTTCGTGCACTTCTCTGCTATCCAGAACGATGGTTACAAATCTCTGGACGAAGGCCAGAAAGTGTCCTTCGCCATCGAAAGCGGCGCTAAAGGCCCGGCAGCTGgtaacgtaa                                                                             <  1:349366/140‑1 (MQ=255)                                                         caCTCCTGACGATGGCTCTAAAGATGTGTTCGTACACTTCTCTGCTATCCAGAACGATGGTTACAAATCTCTGGACGAAGGCCAGAAAGTGTCCTTCACCATCGAAAGCGGCGCTAAAGGCCCGGCAGCTGgtaacgtaa                                                                             <  2:422766/140‑1 (MQ=255)                                                         caCTCCTGACGATGGCTCTAAAGATGTGTTCGTACACTTCTCTGCTATCCAGAACGATGGTTACAAATCTCTGGACGAAGGCCAGAAAGTGTCCTTCACCATCGAAAGCGGCGCTAAAGGCCCGGCAGCTGgtaacgtaa                                                                             <  2:616645/140‑1 (MQ=255)                                                          aCTCCTGACGATGGCTCTAAAGATGTGTTCGTACACTTCTCTGCTATCCAGAACGATGGTTACAAATCTCTGGACGAAGGCCAGAAAGTGTCCTTCACCATCGAAAGCGGCGCTAAAGGCCCGGCAGCTGgtaacgtaac                                                                            <  2:1065244/140‑1 (MQ=255)                                                           ctctTGACGATGGCTCTAAAGATGTGTTCGTACACTTCTCTGCTATCCAGAACGATGGTTACAAATCTCTGGACGAAGGCCAGAAAGTGTCCTTCACCATCGAAAGCGGCGCTAAAGGCCCGGCAGCTGGTAACGTAAcc                                                                           <  2:825250/140‑1 (MQ=255)                                                            tCCTGACGATGGCTCTAAAGATGTGTTCGTACACTTCTCTGCTATCCAGAACGATGGTTACAAATCTCTGGACGAAGGCCAGAAAGTGTCCTTCACCATCGAAAGCGGCGCTAAAGGCCCGGCAGCTGGTAACGTAACCa                                                                          <  1:926401/140‑1 (MQ=255)                                                             ccTGACGATGGCTCTAAAGATGTGTTCGTACACTTCTCTGCTATCCAGAACGATGGTTACAAATCTCTGGACGAAGGCCAGAAAGTGTCCTTCACCATCGAAAGCGGCGCTAAAGGCCCGGCAGCTGGTAACGTAACCAg                                                                         <  1:429009/140‑1 (MQ=255)                                                                   gATGGCTCTAAAGATGTGTTCGTACACTTCTCTGCTATCCAGAACGATGGTTACAAATCTCTGGACGAAGGCCAGAAAGTGTCCTTCACCATCGAAAGCGGCGCTAAAGGCCCGGCAGCTGGTAACGTAACCAGCCTGTa                                                                   <  1:72904/140‑1 (MQ=255)                                                                     tGGCTCTAAAGATGTGTTCGTACACTTCTCTGCTATCCAGAACGATGGTTACAAATCTCTGGACGAAGGCCAGAAAGTGTCCTTCACCATCGAAAGCGGCGCTAAAGGCCCGGCAGCTGGTAACGTAACCAGCCTGTAAt                                                                 <  1:213973/140‑1 (MQ=255)                                                                      ggCTCTAAAGATGTGTTCGTACACTTCTCTGCTATCCAGAACGATGGTTACAAATCTCTGGACGAAGGCCAGAAAGTGTCCTTCACCATCGAAAGCGGCGCTAAAGGCCCGGCAGCTGgtaacgtaa                                                                             <  1:820135/127‑1 (MQ=255)                                                                      ggCTCTAAAGATGTGTTCGTACACTTCTCTGCTATCCAGAACGATGGTTACAAATCTCTGGACGAAGGCCAGAAAGTGTCCTTCACCATCGAAAGCGGCGCTAAAGGCCCGGCAGCTGgtaacgtaa                                                                             >  2:820135/1‑127 (MQ=255)                                                                      ggCTCTAAAGATGTGTTCGTACACTTCTCTGCTATCCAGAACGATGGTTACAAATCTCTGGACGAAGGCCAGAAAGTGTCCTTCACCATCGAAAGCGGCGCTAAAGGCCCGGCAGCTGGTAACGTAACCAGCCTGTAAtc                                                                >  1:367234/1‑140 (MQ=255)                                                                      ggCTCTAAAGATGTGTTCGTACACTTCTCTGCTATCCAGAACGATGGTTACAAATCTCTGGACGAAGGCCAGAAAGTGTCCTTCACCATCGAAAGCGGCGCTAAAGGCCCGGCAGCTGGTAACGTAACCAGCCTGTAAtc                                                                >  1:1097012/1‑140 (MQ=255)                                                                          ctAAAGATGTGTTCGTACACTTCTCTGCTATCCAGAACGATGGTTACAAATCTCTGGACGAAGGCCAGAAAGTGTCCTTCACCATCGAAAGCGGCGCTAAAGGCCCGGCAGCTGGTAACGTAACCAGCCTGTAATCTCTg                                                            <  2:695990/140‑1 (MQ=255)                                                                                  gtgtTCGTACACTTCTCTGCTATCCAGAACGATGGTTACAAATCTCTGGACGAAGGCCAGAAAGTGTCCTTCACCATCGAAAGCGGCGCTAAAGGCCCGGCAGCTGGTAACGTAACCAGCCTGTAATCTCTGCTTAAAAg                                                    <  1:1077184/140‑1 (MQ=255)                                                                                  gtgtTCGTACACTTCTCTGCTATCCAGAACGATGGTTACAAATCTCTGGACGAAGGCCAGAAAGTGTCCTTCACCATCGAAAGCGGCGCTAAAGGCCCGGCAGCTGGTAACGTAACCAGCCTGTAATCTCTGCTTAAAAg                                                    >  2:884087/1‑140 (MQ=255)                                                                                   tgtTCGTACACTTCTCTGCTATCCAGAACGATGGTTACAAATCTCTGGACGAAGGCCAGAAAGTGTCCTTCACCATCGAAAGCGGCGCTAAAGGCCCGGCAGCTGGTAACGTAACCAGCCTGTAATCTCTGCTTAAAAGc                                                   >  1:958173/1‑140 (MQ=255)                                                                                   tgtTCGTACACTTCTCTGCTATCCAGAACGATGGTTACAAATCTCTGGACGAAGGCCAGAAAGTGTCCTTCACCATCGAAAGCGGCGCTAAAGGCCCGGCAGCTGGTAACGTAACCAGCCTGTAATCTCTGCTTAAAAGc                                                   >  2:211401/1‑140 (MQ=255)                                                                                    gtTCGTACACTTCTCTGCTATCCAGAACGATGGTTACAAATCTCTGGACGAAGGCCAGAAAGTGTCCTTCACCATCGAAAGCGGCGCTAAAGGCCCGGCAGCTGGTAACGTAACCAGCCTGTAATCTCTGCTTAAAAGca                                                  <  2:1147380/140‑1 (MQ=255)                                                                                    gtTCGTACACTTCTCTGCTATCCAGAACGATGGTTACAAATCTCTGGACGAAGGCCAGAAAGTGTCCTTCACCATCGAAAGCGGCGCTAAAGGCCCGGCAGCTGGTAACGTAACCAGCCTGTAATCTCTGCTTAAAAGca                                                  <  1:669343/140‑1 (MQ=255)                                                                                    gtTCGTACACTTCTCTGCTATCCAGAACGATGGTTACAAATCTCTGGACGAAGGCCAGAAAGTGTCCTTCACCATCGAAAGCGGCGCTAAAGGCCCGGCAGCTGGTAACGTAACCAGCCTGTAATCTCTGCTTAAAAGca                                                  <  2:274665/140‑1 (MQ=255)                                                                                    gtTCGTACACTTCTCTGCTATCCAGAACGATGGTTACAAATCTCTGGACGAAGGCCAGAAAGTGTCCTTCACCATCGAAAGCGGCGCTAAAGGCCCGGCAGCTGGTAACGTAACCAGCCTGTAATCTCTGCTTAAAAGca                                                  <  1:211401/140‑1 (MQ=255)                                                                                     ttCGTACACTTCTCTGCTATCCAGAACGATGGTTACAAATCTCTGGACGAAGGCCAGAAAGTGTCCTTCACCATCGAAAGCGGCGCTAAAGGCCCGGCAGCTGGTAACGTAACCAGCCTGTAATCTCTGCTTaa                                                       >  1:403675/1‑134 (MQ=255)                                                                                     ttCGTACACTTCTCTGCTATCCAGAACGATGGTTACAAATCTCTGGACGAAGGCCAGAAAGTGTCCTTCACCATCGAAAGCGGCGCTAAAGGCCCGGCAGCTGGTAACGTAACCAGCCTGTAATCTCTGCTTaa                                                       <  2:403675/134‑1 (MQ=255)                                                                                     ttCGTACACTTCTCTGCTATCCAGAACGATGGTTACAAATCTCTGGACGAAGGCCAGAAAGTGTCCTTCACCATCGAAAGCGGCGCTAAAGGCCCGGCAGCTGGTAACGTAACCAGCCTGTAATCTCTGCTTAAAAGcac                                                 <  2:367234/140‑1 (MQ=255)                                                                                      tCGTACACTTCTCTGCTATCCAGAACGATGGTTACAAATCTCTGGACGAAGGCCAGAAAGTGTCCTTCACCATCGAAAGCGGCGCTAAAGGCCCGGCAGCTGGTAACGTAACCAGCCTGTAATCTCTGCTTAAAAGcaca                                                <  1:884087/140‑1 (MQ=255)                                                                                      tCGTACACTTCTCTGCTATCCAGAACGATGGTTACAAATCTCTGGACGAAGGCCAGAAAGTGTCCTTCACCATCGAAAGCGGCGCTAAAGGCCCGGCAGCTGGTAACGTAACCAGCCTGTAATCTCTGCTTAAAAGcaca                                                <  2:268988/140‑1 (MQ=255)                                                                                         tACACTTCTCTGCTATCCAGAACGATGGTTACAAATCTCTGGACGAAGGCCAGAAAGTGTCCTTCACCATCGAAAGCGGCGCTAAAGGCCCGGCAGCTGGTAACGTAACCAGCCTGTAATCTCTGCTTAAAAGCACAGaa                                             >  2:242940/1‑140 (MQ=255)                                                                                             cTTCTCTGCTATCCAGAACGATGGTTACAAATCTCTGGACGAAGGCCAGAAAGTGTCCTTCACCATCGAAAGCGGCGCTAAAGGCCCGGCAGCTGGTAACGTAACCAGCCTGTAATCTCTGCTTAAAAGca                                                  >  1:523481/1‑131 (MQ=255)                                                                                             cTTCTCTGCTATCCAGAACGATGGTTACAAATCTCTGGACGAAGGCCAGAAAGTGTCCTTCACCATCGAAAGCGGCGCTAAAGGCCCGGCAGCTGGTAACGTAACCAGCCTGTAATCTCTGCTTAAAAGca                                                  <  2:523481/131‑1 (MQ=255)                                                                                               tctcTGCTATCCAGAACGATGGTTACAAATCTCTGGACGAAGGCCAGAAAGTGTCCTTCACCATCGAAAGCGGCGCTAAAGGCCCGGCAGCTGGTAACGTAACCAGCCTGTAATCTCTGCTTAAAAGCACAGaa                                             <  1:825206/134‑1 (MQ=255)                                                                                               tctcTGCTATCCAGAACGATGGTTACAAATCTCTGGACGAAGGCCAGAAAGTGTCCTTCACCATCGAAAGCGGCGCTAAAGGCCCGGCAGCTGGTAACGTAACCAGCCTGTAATCTCTGCTTAAAAGCACAGaa                                             >  2:825206/1‑134 (MQ=255)                                                                                                ctctGCTATCCAGAACGATGGTTACAAATCTCTGGACGAAGGCCAGAAAGTGTCCTTCACCATCGAAAGCGGCGCTAAAGGCCCGGCAGCTGGTAACGTAACCAGCCTGTAATCTCTGCTTAAAAGCACAGAATCTAAGa                                      >  1:173615/1‑140 (MQ=255)                                                                                                ctctGCTATCCAGAACGATGGTTACAAATCTCTGGACGAAGGCCAGAAAGTGTCCTTCACCATCGAAAGCGGCGCTAAAGGCCCGGCAGCTGGTAACGTAACCAGCCTGTAATCTCTGCTTAAAAGCACAGAATCTAAGa                                      >  2:880843/1‑140 (MQ=255)                                                                                                 tctGCTATCCAGAACGATGGTTACAAATCTCTGGACGAAGGCCAGAAAGTGTCCTTCACCATCGAAAGCGGCGCTAAAGGCCCGGCAGCTGGTAACGTAACCAGCCTGTAATCTCTGCTTAAAAGCACAGAATCTAAGAt                                     <  2:244149/140‑1 (MQ=255)                                                                                                   tGCTATCCAGAACGATGGTTACAAATCTCTGGACGAAGGCCAGAAAGTGTCCTTCACCATCGAAAGCGGCGCTAAAGGCCCGGCAGCTGGTAACGTAACCAGCCTGTAATCTCTGCTTAAAAGCACAGAATCTAAGATcc                                   >  2:873505/1‑140 (MQ=255)                                                                                                     cTATCCAGAACGATGGTTACAAATCTCTGGACGAAGGCCAGAAAGTGTCCTTCACCATCGAAAGCGGCGCTAAAGGCCCGGCAGCTGGTAACGTAACCAGCCTGTAATCTCTGCTTAAAAGCACAGAATCTAAGATCCCt                                 <  2:769335/140‑1 (MQ=255)                                                                                                      tATCCAGAACGATGGTTACAAATCTCTGGACGAAGGCCAGAAAGTGTCCTTCACCATCGAAAGCGGCGCTAAAGGCCCGGCAGCTGGTAACGTAACCAGCCTGTAATCTCTGCTTAAAAGCACAGAATCTAAGATCCCTg                                <  1:242940/140‑1 (MQ=255)                                                                                                        tCCAGAACGATGGTTACAAATCTCTGGACGAAGGCCAGAAAGTGTCCTTCACCATCGAAAGCGGCGCTAAAGGCCCGGCAGCTGGTAACGTAACCAGCCTGTAATCTCTGCTTAAAAGCACAGAATCt                                          >  2:405285/1‑128 (MQ=255)                                                                                                        tCCAGAACGATGGTTACAAATCTCTGGACGAAGGCCAGAAAGTGTCCTTCACCATCGAAAGCGGCGCTAAAGGCCCGGCAGCTGGTAACGTAACCAGCCTGTAATCTCTGCTTAAAAGCACAGAATCt                                          <  1:405285/128‑1 (MQ=255)                                                                                                         ccAGAACGATGGTTACAAATCTCTGGACGAAGGCCAGAAAGTGTCCTTCACCATCGAAAGCGGCGCTAAAGGCCCGGCAGCTGGTAACGTAACCAGCCTGTAATCTCTGCTTAAAAGCACAGAATCTAAGATCCCTGCCa                             <  2:173615/140‑1 (MQ=255)                                                                                                          cAGAACGATGGTTACAAATCTCTGGACGAAGGCCAGAAAGTGTCCTTCACCATCGAAAGCGGCGCTAAAGGCCCGGCAGCTGGTAACGTAACCAGCCTGTAATCTCTGCTTAAAAGCACAGAATCTAAGATCCCTGCCAt                            >  1:12602/1‑140 (MQ=255)                                                                                                               cGATGGTTACAAATCTCTGGACGAAGGCCAGAAAGTGTCCTTCACCATCGAAAGCGGCGCTAAAGGCCCGGCAGCTGGTAACGTAACCAGCCTGTAATCTCTGCTTAAAAGCACAGAATCTAAGATCCCTGCCATTTGGc                       <  1:873505/140‑1 (MQ=255)                                                                                                                  tGGTTACAAATCTCTGGACGAAGGCCAGAAAGTGTCCTTCACCATCGAAAGCGGCGCTAAAGGCCCGGCAGCTGGTAACGTAACCAGCCTGTAATCTCTGCTTAAAAGCACAGAATCTAAGATCCCTGCCATTTGGCggg                    >  2:955973/1‑140 (MQ=255)                                                                                                                              tctGGACGAAGGCCAGAAAGTGTCCTTCACCATCGAAAGCGGCGCTAAAGGCCCGGCAGCTGGTAACGTAACCAGCCTGTAATCTCTGCTTAAAAGCACAGAATCTAAGATCCCTGCCATTTGGCGGGGATTTTTTTAtt        >  2:1124199/1‑140 (MQ=255)                                                                                                                              tctGGACGAAGGCCAGAAAGTGTCCTTCACCATCGAAAGCGGCGCTAAAGGCCCGGCAGCTGGTAACGTAACCAGCCTGTAATCTCTGCTTAAAAGCACAGAATCTAAGATCCCTGCCATTTGGCGGGGATTTTTTTAtt        >  1:868457/1‑140 (MQ=255)                                                                                                                               ctGGACGAAGGCCAGAAAGTGTCCTTCACCATCGAAAGCGGCGCTAAAGGCCCGGCAGCTGGTAACGTAACCAGCCTGTAATCTCTGCTTAAAAGCACAGAATCTAAGATCCCTGCCATTTgg                        >  2:459859/1‑123 (MQ=255)                                                                                                                               ctGGACGAAGGCCAGAAAGTGTCCTTCACCATCGAAAGCGGCGCTAAAGGCCCGGCAGCTGGTAACGTAACCAGCCTGTAATCTCTGCTTAAAAGCACAGAATCTAAGATCCCTGCCATTTgg                        <  1:459859/123‑1 (MQ=255)                                                                                                                               ctGGACGAAGGCCAGAAAGTGTCCTTCACCATCGAAAGCGGCGCTAAAGGCCCGGCAGCTGGTAACGTAACCAGCCTGTAATCTCTGCTTAAAAGCACAGAATCTAAGATCCCTGCCATTTGGCGGGGATTTTTTTAttt       >  2:132469/1‑140 (MQ=255)                                                                                                                                tGGACGAAGGCCAGAAAGTGTCCTTCACCATCGAAAGCGGCGCTAAAGGCCCGGCAGCTGGTAACGTAACCAGCATGTAATCTCTGCTTAAAAGCACAGAATCTAAGATCCCTGCCATTTGGCGGGGATTTTTTTATTTg      >  2:1140770/1‑140 (MQ=255)                                                                                                                                    cGAAGGCCAGAAAGTGTCCTTCACCATCGAAAGCGGCGCTAAAGGCCCGGCAGCTGGTAACGTAACCAGCCTGTAATCTCTGCTTAAAAGCACAGAATCTAAGATCCCTGCCATTTGGCGGGGATTTTTTTATTTGtttt  <  1:436367/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                      GGTAAAATGACTGGTATCGTAAAATGGTTCAACGCTGACAAAGGCTTCGGCTTCATCACTCCTGACGATGGCTCTAAAGATGTGTTCGTACACTTCTCTGCTATCCAGAACGATGGTTACAAATCTCTGGACGAAGGTCAGAAAGTGTCCTTCACCATCGAAAGCGGCGCTAAAGGCCCGGCAGCTGGTAACGTAACCAGCCTGTAATCTCTGCTTAAAAGCACAGAATCTAAGATCCCTGCCATTTGGCGGGGATTTTTTTATTTGTTTT  >  NC_000913/3720055‑3720325

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑