Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,781,338	A→G	K42E (AAG→GAG)	trmL →	tRNA (cytidine/uridine‑2'‑O)‑ribose methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,781,338	0	A	G	100.0%	152.9 / NA	43	K42E (AAG→GAG)	trmL	tRNA (cytidine/uridine‑2'‑O)‑ribose methyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/13);  total (30/13)

GGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAGGTACACCTGCTCACA  >  NC_000913/3781209‑3781473                                                                                                                                  |                                                                                                                                        ggCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATGAGCGCCTgcg                                                                                                                               <  2:565066/140‑1 (MQ=255)      tATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATGAGCGCCTGCGCcgcg                                                                                                                          <  2:247319/140‑1 (MQ=255)      tATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATGAGCGCCTGCGCcgcg                                                                                                                          <  1:500285/140‑1 (MQ=255)      tATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATGAGCGCCTGCGCcgcg                                                                                                                          <  1:555699/140‑1 (MQ=255)      tATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATGAGCGCCTGCGCcgcg                                                                                                                          <  2:207423/140‑1 (MQ=255)      tATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATGAGCGCCTGCGCcgcg                                                                                                                          <  2:356007/140‑1 (MQ=255)       aTGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATGAGCGCCTGCGCcgcgc                                                                                                                         >  1:21759/1‑140 (MQ=255)           tAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATGAGCGCCTGCGCCGCGCgggg                                                                                                                     <  2:1078238/140‑1 (MQ=255)           tAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATGAGCGCCTGCGCCGCGCgggg                                                                                                                     >  1:257417/1‑140 (MQ=255)           tAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATGAGCGCCTGCGCCGCGCgggg                                                                                                                     >  1:165979/1‑140 (MQ=255)                aTCGTACTTTACGAACTAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATGAGCGCCTGCGCCGCGCgggg                                                                                                                     >  2:537877/1‑135 (MQ=255)                aTCGTACTTTACGAACTAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATGAGCGCCTGCGCCGCGCgggg                                                                                                                     <  1:537877/135‑1 (MQ=255)                aTCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATGAGCGCCTGCGCCGCGCGGGGCTGGa                                                                                                                >  2:806923/1‑140 (MQ=255)                aTCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATGAGCGCCTGCGCCGCGCGGGGCTGGa                                                                                                                >  1:708523/1‑140 (MQ=255)                  cGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATGAGCGCCTGCGCCGCGCGGGGCTGGACt                                                                                                              >  1:292963/1‑140 (MQ=255)                  cGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATGAGCGCCTGCGCCGCGCGGGGCTGGACt                                                                                                              >  1:507908/1‑140 (MQ=255)                  cGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGACTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATGAGCGCCTGCGCCGCGCGGGGCTGGACt                                                                                                              >  1:1010534/1‑140 (MQ=255)                         tACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATGAGCGCCTGCGCCGCGCGGGGCTGGACt                                                                                                              <  2:229401/133‑1 (MQ=255)                         tACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATGAGCGCCTGCGCCGCGCGGGGCTGGACt                                                                                                              >  1:229401/1‑133 (MQ=255)                                  gAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATGAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTAccgc                                                                                              >  1:712248/1‑140 (MQ=255)                                   aaaTTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATGAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTAccgcc                                                                                             >  1:920198/1‑140 (MQ=255)                                      tcccGCAAAATACGGCCAACATCATCCTTTTTGGCACAAACCCCGGCTTTCGTCTGCATATCATCGAACCGATGGGTTTTGCCGGGGAGGATGCGCGCCCGCGCCGGGGGGGGCTGGACTACCACGTTTTTACCGCCGtt                                                                                          <  2:292963/138‑1 (MQ=255)                                          gCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATGAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGATTTTACCGCCGTTAcgc                                                                                      <  2:165979/140‑1 (MQ=255)                                           ccAAATACTGGCAACATCATCCGTCTTTGCGCTAATCCCGGCTTTCGTCTGCATATCACCGAACCGATGGGTTTTGCCTGGGACGAGGAGCGCCGGCGCCGGGGGGGGCTGGACTATCACGAGTTTGCCGCCGTTAcgcg                                                                                     <  2:844972/140‑1 (MQ=255)                                           caaaaTCCTGGCAACATCATCCGTCTTTGCGCTAATCCCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATGAGCGCCTGCGCCGCGGGGGGCTGGACTATCACGAGTTTACCGCCGTTAcgcg                                                                                     <  2:920198/138‑1 (MQ=255)                                                     gCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATGAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGAc                                                                           >  1:745988/1‑140 (MQ=255)                                                     gCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATGAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGAc                                                                           >  1:50777/1‑140 (MQ=255)                                                         catcatCTGTCTTTGCGCTAATCCCGCCTTTCGTCTGCATATCATCGACCCGATGGGTTTTGCCTGGGACGATGAGCGCCTGCGCCGCGGGGGGCTGGACTATCACGATTTTACCGCCGTTACGCGTCATCATGACTATc                                                                       <  2:1010534/140‑1 (MQ=255)                                                             atcCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATGAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATcgcgc                                                                   >  2:225502/1‑140 (MQ=255)                                                              tcCGTCTTTGCGCTAATCCCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCGGGGACGATGAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATcgcgcg                                                                  <  1:60300/140‑1 (MQ=255)                                                               ccGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATGAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGt                                                                 >  2:371658/1‑140 (MQ=255)                                                               ccGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATGAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGt                                                                 >  1:596138/1‑140 (MQ=255)                                                                      tGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATGAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGa                                                          >  1:171381/1‑140 (MQ=255)                                                                      tGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATGAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGa                                                          >  1:872608/1‑140 (MQ=255)                                                                      tGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATGAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGa                                                          >  1:743539/1‑140 (MQ=255)                                                                       gcgtTAATACCGGTTTTCTTCTCCATATCATCGAACCGATGGGATTTCCTTGGGACGATGAGCGCCCGCGCCGCGGGGGGCGGGACTACCAGGAGTTTGCCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGaa                                                         <  2:719972/140‑1 (MQ=255)                                                                              tcccGGCTTGCGTCTGCATATCCTCGACCCGATGGGTTTTGCCTGGGACGATGCGCGCCTGCGCCGCGGGGGGCTGGACTATCACGATTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGaaa                                                  <  2:745988/138‑1 (MQ=255)                                                                                   gCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATGAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATccc                                             >  2:182110/1‑140 (MQ=255)                                                                                       tCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATGAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAgc                                         <  1:464358/140‑1 (MQ=255)                                                                                       tCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATGAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAgc                                         >  1:379372/1‑140 (MQ=255)                                                                                       tCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATGAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAgc                                         >  1:792515/1‑140 (MQ=255)                                                                                        cGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATGAGCGCCTGCGCCGCGGGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAgcg                                        <  2:792515/140‑1 (MQ=255)                                                                                        cGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATGAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAgcg                                        <  2:379372/140‑1 (MQ=255)                                                                                             gCATATCATCGACCCGAGGGGATTTGCCGGGGCCGAGGAGCGCCTGCGCCGCGGGGGGCTGGACTACCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGt                                   <  2:593222/140‑1 (MQ=255)                                                                                                   catcGAACCGATGGGATTTGCCTGGGACGATGAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGccc                             >  1:847486/1‑140 (MQ=255)                                                                                                          ccGATGGGATTTGCCTGGGACGATGAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTcaccac                      >  1:1122840/1‑140 (MQ=255)                                                                                                           ggttGGGTTTTGCCGGGGACGATGGGCGCCTGCGCCGCGCGGGGCTGGCCTTTCGCGATTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACg                     <  2:1122840/137‑1 (MQ=255)                                                                                                             aTGGGATTTGCCTGGGACGATGAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGaa                   >  2:956359/1‑140 (MQ=255)                                                                                                             aTGGGATTTGCCTGGGACGATGAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCACCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGaa                   >  1:617249/1‑140 (MQ=255)                                                                                                             aTGGGATTTGCCTGGGACGATGAGCGCCGGCGCCGCGCGGGGCTGGACTATCCCGAGTTTACCGCCGTTACGAGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGaa                   <  1:480803/140‑1 (MQ=255)                                                                                                             aTGGGATTTGCCCGGGACGATGAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGaa                   >  1:210149/1‑140 (MQ=255)                                                                                                                          gggACGATGAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATGATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAGGTACACCTGCt      >  1:1011030/1‑140 (MQ=255)                                                                                                                           ggACGATGAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAGGTACACCTGCTc     >  1:771901/1‑140 (MQ=255)                                                                                                                              cGATGAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAGGTACACCTGCTcaca  <  2:596138/140‑1 (MQ=255)                                                                                                                                  |                                                                                                                                        GGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAGGTACACCTGCTCACA  >  NC_000913/3781209‑3781473

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑